##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543820_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26357951
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.662866282739504	32.0	32.0	32.0	32.0	32.0
2	30.9049936013615	32.0	32.0	32.0	32.0	32.0
3	33.62669283359697	37.0	32.0	37.0	27.0	37.0
4	34.82651485314621	37.0	37.0	37.0	27.0	37.0
5	35.34426363414971	37.0	37.0	37.0	32.0	37.0
6	38.493235039400446	41.0	41.0	41.0	32.0	41.0
7	38.466138623597864	41.0	41.0	41.0	32.0	41.0
8	38.50905588222696	41.0	41.0	41.0	32.0	41.0
9	38.60476233528168	41.0	41.0	41.0	32.0	41.0
10-11	38.57176625755166	41.0	41.0	41.0	32.0	41.0
12-13	38.52708632397109	41.0	41.0	41.0	32.0	41.0
14-15	38.567761014503745	41.0	41.0	41.0	32.0	41.0
16-17	38.454754980764626	41.0	41.0	41.0	32.0	41.0
18-19	38.49396834753961	41.0	41.0	41.0	32.0	41.0
20-21	38.52721708147951	41.0	41.0	41.0	32.0	41.0
22-23	38.46113954381356	41.0	41.0	41.0	32.0	41.0
24-25	38.44753471163217	41.0	41.0	41.0	32.0	41.0
26-27	38.51881346922604	41.0	41.0	41.0	32.0	41.0
28-29	38.41793793075949	41.0	41.0	41.0	32.0	41.0
30-31	38.431869704135956	41.0	41.0	41.0	32.0	41.0
32-33	38.59732380183877	41.0	41.0	41.0	32.0	41.0
34-35	38.563059814474954	41.0	41.0	41.0	32.0	41.0
36-37	38.456686770389396	41.0	41.0	41.0	32.0	41.0
38-39	38.40950838550303	41.0	41.0	41.0	32.0	41.0
40-41	38.41917330067065	41.0	41.0	41.0	32.0	41.0
42-43	38.375811185656005	41.0	41.0	41.0	32.0	41.0
44-45	38.329306578025694	41.0	39.0	41.0	32.0	41.0
46-47	38.33603207680328	41.0	41.0	41.0	32.0	41.0
48-49	38.301522670655174	41.0	37.0	41.0	32.0	41.0
50-51	38.24097297595327	41.0	37.0	41.0	32.0	41.0
52-53	38.26956220815528	41.0	37.0	41.0	32.0	41.0
54-55	38.27167900925704	41.0	37.0	41.0	32.0	41.0
56-57	38.25739395659594	41.0	37.0	41.0	32.0	41.0
58-59	38.26887908578333	41.0	37.0	41.0	32.0	41.0
60-61	38.21805054483288	41.0	37.0	41.0	32.0	41.0
62-63	38.251950261002875	41.0	37.0	41.0	32.0	41.0
64-65	38.240772256488455	41.0	37.0	41.0	32.0	41.0
66-67	38.15762356581919	41.0	37.0	41.0	32.0	41.0
68-69	38.16080584984644	41.0	37.0	41.0	32.0	41.0
70-71	37.983212740781866	41.0	37.0	41.0	32.0	41.0
72-73	38.089908396968966	41.0	37.0	41.0	32.0	41.0
74-75	38.10778476245794	41.0	37.0	41.0	32.0	41.0
76	37.2209773423614	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13432.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	44.0
13	249.0
14	1238.0
15	4555.0
16	14490.0
17	33408.0
18	55263.0
19	75338.0
20	90328.0
21	101037.0
22	111494.0
23	123886.0
24	137235.0
25	153038.0
26	171808.0
27	192924.0
28	219016.0
29	251450.0
30	288989.0
31	336224.0
32	392955.0
33	467438.0
34	569417.0
35	718712.0
36	953085.0
37	1373248.0
38	2353372.0
39	5884224.0
40	1.1270054E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.479447041488406	19.852789657872474	14.27174966177412	31.396013638865007
2	21.66764422471867	28.02377976637661	28.95927830558902	21.3492977033157
3	20.62219849221768	28.96008463847831	28.13679763900795	22.28091923029606
4	24.39727390696905	33.10542630562406	19.89195581482725	22.605343972579643
5	23.760166002573367	35.4424103413311	20.450701511496796	20.346722144598736
6	19.291705976182666	37.48426512227939	21.214414844751104	22.00961405678684
7	19.306396002255653	21.76894068338325	34.282833549495194	24.641829764865896
8	20.41433319864113	24.028478574735097	26.698102016719925	28.859086209903843
9	21.33235959670176	23.682286879620122	28.093091378076856	26.892262145601258
10-11	23.04576597748749	31.290236903744518	21.86889407441143	23.795103044356562
12-13	22.83493159917191	25.537340994745357	26.06273357112878	25.56499383495396
14-15	21.921848147451044	28.347115172502658	26.600020349682396	23.131016330363906
16-17	22.30710874737412	28.40733261212611	26.017031531486307	23.26852710901347
18-19	21.96694191598396	28.620490668912247	26.270661634655557	23.141905780448234
20-21	22.083598500642445	28.51493989202526	26.242816900627886	23.15864470670441
22-23	22.36690213128277	28.195857042242256	26.282086965638424	23.155153860836553
24-25	22.291745565858314	28.084729916643163	26.28382496486109	23.33969955263743
26-27	21.91745789864898	28.51301467532346	26.286754856615968	23.28277256941159
28-29	22.70697908555953	27.9403731855624	26.121491822844256	23.231155906033816
30-31	22.41357710866206	27.97463536048184	26.36079457795174	23.25099295290436
32-33	22.079218791661035	28.336251159888732	26.430032090966787	23.154497957483446
34-35	22.347455988382407	28.38777858096911	25.881571672005787	23.383193758642697
36-37	22.04602390178536	28.005579662701287	26.43885208278031	23.50954435273304
38-39	21.67320509235639	28.319028313975895	26.723211190001074	23.28455540366664
40-41	22.27047159359402	28.037068145835825	26.21933455996549	23.47312570060466
42-43	21.857019157041844	28.018931840050588	26.450271634233086	23.673777368674482
44-45	22.00927305303714	27.884358099014438	26.474865206197943	23.631503641750474
46-47	22.223063610060432	27.838823357314784	26.210555512282973	23.727557520341808
48-49	22.101302245810928	27.740936814996086	26.548961473897116	23.60879946529587
50-51	22.02725187372337	27.700684240165117	26.685529904693578	23.586533981417933
52-53	22.11316003465469	27.812948630544792	26.172664999311635	23.901226335488886
54-55	22.073489346844294	27.470815849067108	26.699852628854266	23.755842175234328
56-57	21.780012106047085	28.015553022410266	26.41443686549548	23.789998006047163
58-59	21.911073082528585	28.090188099660008	26.49189619532078	23.50684262249063
60-61	21.99158857169326	27.62071572487526	26.862336418049388	23.52535928538209
62-63	21.86792737044793	27.97122765964794	26.63406353343913	23.526781436464997
64-65	22.27326861543302	27.71294369004095	26.51000715426772	23.503780540258305
66-67	22.204257117168893	27.67799016114155	26.805979804994973	23.311772916694583
68-69	21.977452474420588	27.678980212109245	26.986387222977132	23.357180090493035
70-71	22.32003626232243	27.851149676537858	26.66167519773846	23.167138863401256
72-73	22.046791992225202	27.69352248559562	26.651491852419806	23.608193669759373
74-75	21.925950016944288	24.525935180583755	28.384388111001446	25.16372669147051
76	23.191616727272294	0.0	40.04659820045616	36.76178507227154
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	65886.0
1	40221.5
2	13558.0
3	12422.5
4	12286.0
5	11333.0
6	11996.0
7	16162.0
8	18716.0
9	20212.0
10	26192.5
11	63149.5
12	95629.0
13	119720.5
14	107963.0
15	57942.5
16	31797.5
17	20568.5
18	20969.0
19	23533.5
20	31221.0
21	38590.0
22	47301.5
23	64200.5
24	89341.0
25	112138.5
26	129479.5
27	162450.5
28	208497.0
29	238010.5
30	292285.0
31	386591.5
32	477891.5
33	538866.0
34	645252.5
35	778394.0
36	855549.5
37	892457.0
38	911501.0
39	974166.5
40	1045534.5
41	1080428.5
42	1094380.0
43	1153287.5
44	1262896.5
45	1329178.0
46	1361584.0
47	1457357.5
48	1513294.5
49	1461704.5
50	1410810.5
51	1369040.0
52	1328119.5
53	1265363.0
54	1194052.5
55	1089409.0
56	952016.0
57	809320.0
58	709554.5
59	628076.5
60	517047.0
61	421395.5
62	356886.0
63	299607.5
64	231821.0
65	179387.0
66	134342.0
67	111495.0
68	96226.5
69	76018.0
70	58866.0
71	48613.5
72	43678.0
73	40806.0
74	35116.0
75	28554.5
76	23263.5
77	17216.5
78	14113.5
79	12941.5
80	12045.5
81	10581.0
82	9104.0
83	8317.0
84	7240.5
85	5542.0
86	4267.0
87	3686.0
88	3368.0
89	2208.5
90	1043.5
91	672.0
92	593.5
93	512.5
94	400.5
95	365.0
96	359.0
97	323.5
98	254.0
99	200.5
100	182.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.205180933829037
2	0.18665335556621984
3	0.05095995511942488
4	0.05208674983878678
5	0.054112703980669814
6	0.05163147924510521
7	0.05119897218110771
8	0.052872091612887515
9	0.0533880649523933
10-11	0.05184393885548994
12-13	0.051066184924617244
14-15	0.05144368012521155
16-17	0.05196344738633135
18-19	0.05340703456046336
20-21	0.052107616407663856
22-23	0.05126157188773893
24-25	0.056910721171004534
26-27	0.053761766231373596
28-29	0.05628851802630636
30-31	0.061913006819080886
32-33	0.06882173807819887
34-35	0.07573426325893087
36-37	0.0030489237982437136
38-39	0.003914946626385878
40-41	0.0010956247828806823
42-43	0.0022789340012736962
44-45	0.0017056865900420839
46-47	1.557810479892637E-4
48-49	0.004136705479871137
50-51	0.003962921048006921
52-53	0.0015856625381958822
54-55	0.010027140990503506
56-57	0.004197418960274208
58-59	0.004178383749388043
60-61	0.001262749652796222
62-63	3.1064300873038354E-4
64-65	6.71310034259888E-4
66-67	0.005388286757864422
68-69	0.003809436329130867
70-71	0.004042838405320177
72-73	0.0014790547787337893
74-75	2.825575446355437E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	20334.0
36	910.0
37	1104.0
38	1267.0
39	1498.0
40	1661.0
41	1955.0
42	2246.0
43	2191.0
44	2149.0
45	2299.0
46	2692.0
47	2865.0
48	3140.0
49	3434.0
50	3706.0
51	4139.0
52	4411.0
53	4809.0
54	4982.0
55	5270.0
56	5671.0
57	5897.0
58	6368.0
59	6907.0
60	7623.0
61	8263.0
62	8513.0
63	9277.0
64	9951.0
65	10790.0
66	11837.0
67	11937.0
68	12008.0
69	13329.0
70	15041.0
71	37841.0
72	138996.0
73	606587.0
74	1868497.0
75	6680186.0
76	1.680537E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.921876207856528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.22735361190217	21.00488854996181
2	11.00602648515255	6.146178181176396
3	4.357654664302559	3.6502168227973386
4	2.2354281412467167	2.4966939132579857
5	1.3337950911100034	1.8621030710310116
6	0.9429488308522945	1.5797340315240482
7	0.6807980477018757	1.3306411167337524
8	0.4899389183972813	1.0944011063120007
9	0.3897011362038708	0.9793068194830964
>10	2.7058998098310987	14.857693552773696
>50	0.3090874491321298	6.016572715297632
>100	0.2588239714214872	14.771168077573785
>500	0.03558179081554618	6.898941924814367
>1k	0.02563121206232696	13.328939710579867
>5k	0.0010446377459534027	2.0267895241458627
>10k+	2.8620212217900915E-4	1.9557308825370594
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	130572	0.4953799329849274	No Hit
CCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	56786	0.21544163277335177	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39095	0.14832336549984482	No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34484	0.13082959293763008	No Hit
CCCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29736	0.11281605311429556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	3.793921614013168E-6	0.0	0.0	3.793921614013168E-6	0.0
5	3.793921614013168E-6	0.0	0.0	3.793921614013168E-6	0.0
6	7.587843228026336E-6	0.0	0.0	7.587843228026336E-6	0.0
7	7.587843228026336E-6	0.0	0.0	5.311490259618436E-5	0.0
8	7.587843228026336E-6	0.0	0.0	6.070274582421069E-5	0.0
9	7.587843228026336E-6	0.0	0.0	6.449666743822386E-5	7.587843228026336E-6
10	1.5175686456052672E-5	0.0	0.0	6.449666743822386E-5	7.587843228026336E-6
11	1.5175686456052672E-5	0.0	0.0	6.449666743822386E-5	7.587843228026336E-6
12	1.5175686456052672E-5	0.0	0.0	6.829058905223703E-5	7.587843228026336E-6
13	1.5175686456052672E-5	0.0	0.0	6.829058905223703E-5	7.587843228026336E-6
14	1.896960807006584E-5	0.0	0.0	6.829058905223703E-5	7.587843228026336E-6
15	2.276352968407901E-5	0.0	0.0	6.829058905223703E-5	7.587843228026336E-6
16	3.414529452611851E-5	0.0	0.0	6.829058905223703E-5	7.587843228026336E-6
17	4.173313775414485E-5	0.0	0.0	6.829058905223703E-5	7.587843228026336E-6
18	4.552705936815802E-5	0.0	0.0	6.829058905223703E-5	7.587843228026336E-6
19	4.552705936815802E-5	0.0	0.0	7.20845106662502E-5	7.587843228026336E-6
20	5.311490259618436E-5	0.0	0.0	8.34662755082897E-5	7.587843228026336E-6
21	5.311490259618436E-5	0.0	0.0	8.34662755082897E-5	1.1381764842039505E-5
22	5.690882421019752E-5	0.0	0.0	8.34662755082897E-5	1.1381764842039505E-5
23	6.449666743822386E-5	0.0	0.0	9.105411873631604E-5	1.1381764842039505E-5
24	7.20845106662502E-5	0.0	0.0	9.105411873631604E-5	1.1381764842039505E-5
25	7.20845106662502E-5	0.0	0.0	9.484804035032921E-5	1.1381764842039505E-5
26	7.587843228026336E-5	0.0	0.0	9.484804035032921E-5	1.1381764842039505E-5
27	7.967235389427653E-5	0.0	0.0	9.484804035032921E-5	1.1381764842039505E-5
28	9.105411873631604E-5	0.0	0.0	9.484804035032921E-5	1.1381764842039505E-5
29	9.105411873631604E-5	0.0	0.0	9.484804035032921E-5	1.1381764842039505E-5
30	1.0622980519236872E-4	0.0	0.0	9.484804035032921E-5	1.1381764842039505E-5
31	1.3278725649046088E-4	0.0	0.0	9.484804035032921E-5	1.1381764842039505E-5
32	1.5175686456052673E-4	0.0	0.0	9.484804035032921E-5	1.1381764842039505E-5
33	1.555507861745399E-4	0.0	0.0	9.864196196434238E-5	1.1381764842039505E-5
34	1.6313862940256623E-4	0.0	0.0	1.0622980519236872E-4	1.1381764842039505E-5
35	1.669325510165794E-4	0.0	0.0	1.0622980519236872E-4	1.1381764842039505E-5
36	1.7452039424460574E-4	0.0	0.0	1.2519941326243455E-4	1.1381764842039505E-5
37	1.9728392392868475E-4	0.0	0.0	1.4037509971848722E-4	1.1381764842039505E-5
38	2.1245961038473743E-4	0.0	0.0	1.4796294294651356E-4	1.1381764842039505E-5
39	2.5798666975289543E-4	0.0	0.0	1.4796294294651356E-4	1.1381764842039505E-5
40	2.6936843459493494E-4	0.0	0.0	1.6313862940256623E-4	1.1381764842039505E-5
41	2.9213196427901395E-4	0.0	0.0	1.783143158586189E-4	1.1381764842039505E-5
42	2.959258858930271E-4	0.0	0.0	1.8210823747263208E-4	1.1381764842039505E-5
43	3.0351372912105346E-4	0.0	0.0	1.8969608070065842E-4	1.1381764842039505E-5
44	3.2627725880513247E-4	3.793921614013168E-6	0.0	1.8969608070065842E-4	1.1381764842039505E-5
45	3.60422553331251E-4	3.793921614013168E-6	0.0	1.9728392392868475E-4	1.1381764842039505E-5
46	3.6421647494526416E-4	3.793921614013168E-6	0.0	2.238413752267769E-4	1.1381764842039505E-5
47	3.718043181732905E-4	3.793921614013168E-6	0.0	2.4281098329684275E-4	1.1381764842039505E-5
48	4.1353745592743535E-4	3.793921614013168E-6	0.0	2.466049049108559E-4	1.1381764842039505E-5
49	4.173313775414485E-4	3.793921614013168E-6	0.0	2.5419274813888226E-4	1.1381764842039505E-5
50	4.476827504535538E-4	3.793921614013168E-6	0.0	2.617805913669086E-4	1.1381764842039505E-5
51	4.74240201751646E-4	3.793921614013168E-6	0.0	2.9213196427901395E-4	1.1381764842039505E-5
52	4.932098098217118E-4	3.793921614013168E-6	0.0	3.0351372912105346E-4	1.1381764842039505E-5
53	5.159733395057909E-4	7.587843228026336E-6	0.0	3.1868941557710613E-4	1.1381764842039505E-5
54	5.387368691898699E-4	7.587843228026336E-6	0.0	3.2627725880513247E-4	1.1381764842039505E-5
55	5.501186340319094E-4	7.587843228026336E-6	0.0	3.566286317172378E-4	1.1381764842039505E-5
56	5.690882421019752E-4	7.587843228026336E-6	0.0	3.7559823978730366E-4	1.1381764842039505E-5
57	5.880578501720411E-4	7.587843228026336E-6	0.0	3.945678478573695E-4	1.1381764842039505E-5
58	6.335849095401991E-4	7.587843228026336E-6	0.0	4.0215569108539585E-4	1.1381764842039505E-5
59	7.132572634344756E-4	7.587843228026336E-6	0.0	4.05949612699409E-4	1.1381764842039505E-5
60	7.360207931185547E-4	7.587843228026336E-6	0.0	4.05949612699409E-4	1.1381764842039505E-5
61	7.89135695714739E-4	7.587843228026336E-6	0.0	4.211252991554617E-4	1.1381764842039505E-5
62	8.005174605567785E-4	7.587843228026336E-6	0.0	4.2491922076947486E-4	1.1381764842039505E-5
63	8.005174605567785E-4	7.587843228026336E-6	0.0	4.363009856115143E-4	1.1381764842039505E-5
64	8.005174605567785E-4	1.1381764842039505E-5	0.0	4.400949072255275E-4	1.1381764842039505E-5
65	8.043113821707917E-4	1.5175686456052672E-5	0.0	4.4388882883954065E-4	1.5175686456052672E-5
>>END_MODULE
