##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543820_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26357951
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45480322047795	32.0	32.0	32.0	32.0	32.0
2	31.747541074038722	32.0	32.0	32.0	32.0	32.0
3	35.875462284606265	37.0	37.0	37.0	32.0	37.0
4	36.4152787141914	37.0	37.0	37.0	37.0	37.0
5	36.5891550143636	37.0	37.0	37.0	37.0	37.0
6	40.0776555810427	41.0	41.0	41.0	37.0	41.0
7	40.17284427761475	41.0	41.0	41.0	37.0	41.0
8	40.16626508638703	41.0	41.0	41.0	37.0	41.0
9	40.145102326049546	41.0	41.0	41.0	37.0	41.0
10-11	39.85315345642763	41.0	41.0	41.0	37.0	41.0
12-13	39.757219178379984	41.0	41.0	41.0	37.0	41.0
14-15	39.69600573276732	41.0	41.0	41.0	37.0	41.0
16-17	39.67464568850591	41.0	41.0	41.0	37.0	41.0
18-19	39.652063925606356	41.0	41.0	41.0	37.0	41.0
20-21	39.6233084847908	41.0	41.0	41.0	37.0	41.0
22-23	39.61504443194389	41.0	41.0	41.0	37.0	41.0
24-25	39.59197006626198	41.0	41.0	41.0	37.0	41.0
26-27	39.47594803556619	41.0	41.0	41.0	37.0	41.0
28-29	39.4779082220769	41.0	41.0	41.0	37.0	41.0
30-31	39.5121996964028	41.0	41.0	41.0	37.0	41.0
32-33	39.56966182614119	41.0	41.0	41.0	37.0	41.0
34-35	39.57640920191406	41.0	41.0	41.0	37.0	41.0
36-37	39.72419175020005	41.0	41.0	41.0	37.0	41.0
38-39	39.79157258865286	41.0	41.0	41.0	37.0	41.0
40-41	39.86755620686674	41.0	41.0	41.0	37.0	41.0
42-43	39.89241639719873	41.0	41.0	41.0	37.0	41.0
44-45	39.90811929041774	41.0	41.0	41.0	37.0	41.0
46-47	39.82638621406287	41.0	41.0	41.0	37.0	41.0
48-49	39.89333645921265	41.0	41.0	41.0	37.0	41.0
50-51	39.87711116032724	41.0	41.0	41.0	37.0	41.0
52-53	39.82587715140012	41.0	41.0	41.0	37.0	41.0
54-55	39.870839230884044	41.0	41.0	41.0	37.0	41.0
56-57	39.873296331959665	41.0	41.0	41.0	37.0	41.0
58-59	39.80371250128941	41.0	41.0	41.0	37.0	41.0
60-61	39.836626589833116	41.0	41.0	41.0	37.0	41.0
62-63	39.82276242415145	41.0	41.0	41.0	37.0	41.0
64-65	39.765450993674406	41.0	41.0	41.0	37.0	41.0
66-67	39.76551470414068	41.0	41.0	41.0	37.0	41.0
68-69	39.75306130866986	41.0	41.0	41.0	37.0	41.0
70-71	39.73483091893503	41.0	41.0	41.0	37.0	41.0
72-73	39.68503177250357	41.0	41.0	41.0	37.0	41.0
74-75	39.671721135321604	41.0	41.0	41.0	37.0	41.0
76	39.17394552951999	41.0	41.0	41.0	37.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13984.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.0
15	2.0
16	8.0
17	23.0
18	67.0
19	189.0
20	413.0
21	1009.0
22	2288.0
23	4337.0
24	7442.0
25	12701.0
26	21247.0
27	34152.0
28	52608.0
29	78198.0
30	111140.0
31	155266.0
32	210357.0
33	281797.0
34	374906.0
35	500122.0
36	684865.0
37	994725.0
38	1704646.0
39	4983083.0
40	1.6128374E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.47009713441794	11.076067642386443	12.052107615183754	35.40172760801185
2	22.93780033732467	15.68282836485195	33.97317939573482	27.406191902088565
3	22.246342018269306	20.2552485736108	27.69154698683004	29.806862421289853
4	26.917162551629126	27.17199549631134	21.12013823723008	24.790703714829455
5	26.127222980502516	30.335696973808083	24.53659693697612	19.00048310871328
6	21.658073854393027	32.86154826558253	26.759972710208586	18.720405169815862
7	18.364170437960237	22.88577874395303	42.13842964501132	16.611621173075413
8	20.96723040124015	22.900907720607343	33.75750112008856	22.374360758063943
9	21.237701216373374	22.222693339996972	35.26077906186263	21.27882638176703
10-11	23.873369185438168	30.35565790072543	26.394156962009557	19.376815951826845
12-13	23.77458235960095	24.34749752271549	30.434620538128698	21.443299579554868
14-15	22.627505091924725	26.359267699678014	30.57743478822256	20.435792420174696
16-17	23.061470366362276	26.54670833248399	29.939217041427092	20.452604259726645
18-19	23.08990313341215	26.724365523564884	29.75541077434623	20.430320568676734
20-21	22.614155380272717	26.70065914914393	30.075468526715916	20.60971694386744
22-23	23.110719993665324	26.39139323816301	29.543549023499434	20.954337744672234
24-25	23.076005175463354	26.55922031724292	29.41151026238506	20.953264244908667
26-27	22.80667027963325	26.86087662292365	29.46131093438312	20.871142163059975
28-29	23.283115936283483	26.50200656166935	29.503160042543282	20.711717459503884
30-31	22.974019851984238	26.71703787156698	29.279742489638622	21.029199786810164
32-33	22.88800243845477	26.458088451237444	29.425189566528292	21.228719543779494
34-35	22.904129683723806	26.774421432919926	29.356626310834617	20.964822572521648
36-37	23.52373404702434	26.280635908043305	29.052159949721872	21.143470095210482
38-39	22.8088791363255	26.849478624300737	29.42074383447001	20.920898404903753
40-41	23.258258482642127	26.285220453971096	29.519138311567712	20.937382751819065
42-43	22.784545980515592	26.02389720666869	29.920655682848345	21.270901129967378
44-45	23.064495973613706	26.272020274987153	29.68053671398701	20.982947037412135
46-47	22.986344816753604	26.459692624538373	29.633868924511887	20.920093634196142
48-49	23.04587674697782	26.308451939402477	29.49005052833647	21.155620785283226
50-51	22.748347454743886	26.545415391469522	29.506600867697923	21.199636286088666
52-53	23.11985412009767	26.198983244002616	29.362271656258216	21.318890979641495
54-55	22.916185435754755	26.582853541557423	28.990590199753914	21.51037082293391
56-57	23.387299292544693	26.164142722510874	29.419373289085904	21.02918469585853
58-59	23.25465745055601	26.14390798945543	29.38519303619241	21.21624152379615
60-61	22.58734639652702	26.280116710090066	29.609864005448877	21.522672887934032
62-63	22.673729083762222	26.494513579681666	29.69819037568432	21.13356696087179
64-65	23.057366986044283	26.337979567989656	29.340040054807986	21.26461339115807
66-67	23.28976000636108	26.213726078016748	29.23698481705694	21.25952909856523
68-69	22.896235410857887	26.6824760200546	29.332587700667684	21.088700868419828
70-71	23.14892706814669	26.40142799943529	29.19205453116625	21.257590401251772
72-73	23.074842737655302	25.994888475881034	29.352148344196944	21.57812044226672
74-75	23.110347745400663	23.038235333331368	30.807911623365868	23.0435052979021
76	24.196050827143768	0.0	43.26708801636251	32.53686115649373
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	401179.0
1	277416.5
2	96737.0
3	36444.0
4	33068.0
5	36692.5
6	50342.0
7	71198.0
8	82028.0
9	81684.0
10	91594.0
11	90328.0
12	78812.5
13	59194.0
14	26798.5
15	11869.5
16	9505.0
17	9693.0
18	11235.0
19	16210.5
20	23174.5
21	28500.0
22	37008.0
23	50012.5
24	64874.0
25	79508.0
26	109499.5
27	163620.5
28	225884.0
29	277908.5
30	338740.5
31	403272.0
32	475299.5
33	546465.5
34	640400.5
35	768216.5
36	879701.5
37	932009.0
38	942970.5
39	1004780.0
40	1082851.0
41	1107259.0
42	1112949.5
43	1174443.5
44	1256484.0
45	1309413.5
46	1324303.0
47	1377366.5
48	1413342.5
49	1365912.5
50	1323805.0
51	1304611.5
52	1298938.5
53	1220072.0
54	1143526.0
55	1068841.5
56	937094.5
57	797256.5
58	698181.0
59	615311.5
60	508604.5
61	408572.0
62	337816.0
63	285076.5
64	222281.5
65	175335.0
66	130048.0
67	102196.0
68	80115.5
69	56245.5
70	42755.0
71	35485.5
72	31006.5
73	25891.0
74	21594.0
75	18327.0
76	16139.0
77	12846.5
78	11599.0
79	12403.0
80	10004.5
81	7627.0
82	6807.5
83	5682.0
84	5443.5
85	4985.5
86	5039.0
87	5511.0
88	3651.0
89	1500.0
90	686.0
91	195.5
92	124.5
93	102.0
94	63.5
95	45.0
96	42.0
97	28.0
98	10.5
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8657008278071388
2	0.11007684170897807
3	0.05305419985036015
4	0.060657218764842535
5	0.05305419985036015
6	0.053243895931060804
7	0.05305419985036015
8	0.053312186520113033
9	0.05305419985036015
10-11	0.05305419985036015
12-13	0.053439282894182476
14-15	0.05323820504863978
16-17	0.05331977436334106
18-19	0.05306178769358817
20-21	0.05307316945843021
22-23	0.05334064093221813
24-25	0.05469317398761383
26-27	0.06342109066065113
28-29	0.06542807519446409
30-31	0.06490641097253728
32-33	0.06950274700791424
34-35	0.07446140255742945
36-37	4.974022691871217E-4
38-39	0.0016119418117485494
40-41	3.9685850604305375E-4
42-43	1.8991207564668956E-6
44-45	0.0
46-47	2.0897432645912287E-5
48-49	6.194581534925335E-4
50-51	5.074786867979725E-4
52-53	2.0723694611149245E-4
54-55	1.711744735191473E-4
56-57	0.0017847320850762377
58-59	0.0021015293765824527
60-61	1.7141063044925106E-5
62-63	0.0027633256902920974
64-65	0.0
66-67	0.001320754634154879
68-69	0.001184378913226135
70-71	1.9121987187503707E-6
72-73	2.1116443247658293E-5
74-75	6.12688148693755E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	20678.0
36	882.0
37	1073.0
38	1241.0
39	1460.0
40	1628.0
41	1920.0
42	2192.0
43	2154.0
44	2113.0
45	2261.0
46	2653.0
47	2829.0
48	3096.0
49	3413.0
50	3669.0
51	4104.0
52	4371.0
53	4781.0
54	4933.0
55	5232.0
56	5628.0
57	5878.0
58	6354.0
59	6868.0
60	7585.0
61	8234.0
62	8438.0
63	9241.0
64	9932.0
65	10412.0
66	11107.0
67	11549.0
68	11972.0
69	13122.0
70	14076.0
71	39718.0
72	110202.0
73	486381.0
74	1881376.0
75	6336841.0
76	1.7286354E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.704626327907544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.00746540053947	15.667887157552574
2	13.85013164748614	6.289241272969988
3	5.255352229177941	3.5796242575506323
4	2.741202772478988	2.4895193855263833
5	1.7674184933875605	2.0064288228698945
6	1.1620418997096902	1.5830236266168196
7	0.8366745085464495	1.3297467452242975
8	0.6414934777175199	1.1651895762692936
9	0.5273151029539207	1.0775243132667782
>10	3.4144332476956687	15.205205207349623
>50	0.39904561544191675	6.321619379139251
>100	0.32097328549481224	14.743122383192746
>500	0.042686328271020584	6.731698945327349
>1k	0.03089435860881114	13.367898045653586
>5k	0.0023078764182163348	3.5283244259561455
>10k+	5.637560716253624E-4	4.913946455534797
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	357456	1.356160044458691	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	112507	0.4268427390277795	No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	93448	0.3545343869863025	No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	75427	0.28616412558017124	No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	71499	0.2712616014803275	No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	67081	0.2545000557896173	No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53980	0.20479588872443083	No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32149	0.12197078596890934	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.587843228026336E-6	7.587843228026336E-6
3	3.793921614013168E-6	0.0	0.0	7.587843228026336E-6	1.1381764842039505E-5
4	3.793921614013168E-6	0.0	0.0	1.5175686456052672E-5	1.1381764842039505E-5
5	3.793921614013168E-6	0.0	0.0	1.5175686456052672E-5	1.1381764842039505E-5
6	3.793921614013168E-6	0.0	0.0	1.5175686456052672E-5	1.5175686456052672E-5
7	3.793921614013168E-6	0.0	0.0	1.5175686456052672E-5	1.5175686456052672E-5
8	3.793921614013168E-6	0.0	0.0	1.896960807006584E-5	1.5175686456052672E-5
9	3.793921614013168E-6	0.0	0.0	2.276352968407901E-5	1.5175686456052672E-5
10	3.793921614013168E-6	0.0	0.0	2.276352968407901E-5	2.276352968407901E-5
11	1.1381764842039505E-5	0.0	0.0	2.276352968407901E-5	2.276352968407901E-5
12	1.1381764842039505E-5	0.0	0.0	2.276352968407901E-5	2.276352968407901E-5
13	1.1381764842039505E-5	0.0	0.0	2.276352968407901E-5	2.655745129809218E-5
14	1.5175686456052672E-5	0.0	0.0	2.276352968407901E-5	2.655745129809218E-5
15	1.896960807006584E-5	0.0	0.0	2.655745129809218E-5	2.655745129809218E-5
16	1.896960807006584E-5	0.0	0.0	2.655745129809218E-5	2.655745129809218E-5
17	1.896960807006584E-5	0.0	0.0	3.414529452611851E-5	3.414529452611851E-5
18	1.896960807006584E-5	0.0	0.0	3.793921614013168E-5	3.414529452611851E-5
19	1.896960807006584E-5	0.0	0.0	3.793921614013168E-5	3.414529452611851E-5
20	1.896960807006584E-5	0.0	0.0	3.793921614013168E-5	3.414529452611851E-5
21	2.276352968407901E-5	0.0	0.0	4.173313775414485E-5	3.414529452611851E-5
22	2.276352968407901E-5	0.0	0.0	4.552705936815802E-5	3.414529452611851E-5
23	2.655745129809218E-5	3.793921614013168E-6	0.0	4.552705936815802E-5	3.414529452611851E-5
24	3.0351372912105344E-5	3.793921614013168E-6	0.0	4.552705936815802E-5	3.414529452611851E-5
25	3.0351372912105344E-5	3.793921614013168E-6	0.0	5.311490259618436E-5	3.793921614013168E-5
26	3.0351372912105344E-5	1.1381764842039505E-5	0.0	5.690882421019752E-5	3.793921614013168E-5
27	3.414529452611851E-5	1.1381764842039505E-5	0.0	5.690882421019752E-5	4.173313775414485E-5
28	3.793921614013168E-5	1.1381764842039505E-5	0.0	5.690882421019752E-5	4.173313775414485E-5
29	3.793921614013168E-5	1.1381764842039505E-5	0.0	6.070274582421069E-5	4.173313775414485E-5
30	3.793921614013168E-5	1.1381764842039505E-5	0.0	6.070274582421069E-5	4.552705936815802E-5
31	3.793921614013168E-5	1.1381764842039505E-5	0.0	6.449666743822386E-5	4.552705936815802E-5
32	3.793921614013168E-5	1.1381764842039505E-5	0.0	6.449666743822386E-5	4.932098098217119E-5
33	4.173313775414485E-5	1.1381764842039505E-5	0.0	6.449666743822386E-5	5.690882421019752E-5
34	4.552705936815802E-5	1.5175686456052672E-5	0.0	6.449666743822386E-5	5.690882421019752E-5
35	4.932098098217119E-5	1.5175686456052672E-5	0.0	6.449666743822386E-5	6.070274582421069E-5
36	5.311490259618436E-5	1.5175686456052672E-5	0.0	7.587843228026336E-5	6.070274582421069E-5
37	5.311490259618436E-5	1.5175686456052672E-5	0.0	8.726019712230287E-5	6.070274582421069E-5
38	6.070274582421069E-5	1.5175686456052672E-5	0.0	9.484804035032921E-5	6.070274582421069E-5
39	6.829058905223703E-5	1.5175686456052672E-5	0.0	9.864196196434238E-5	6.449666743822386E-5
40	6.829058905223703E-5	1.5175686456052672E-5	0.0	1.1002372680638187E-4	6.829058905223703E-5
41	7.20845106662502E-5	1.896960807006584E-5	0.0	1.1761157003440821E-4	6.829058905223703E-5
42	7.20845106662502E-5	1.896960807006584E-5	0.0	1.2140549164842138E-4	6.829058905223703E-5
43	7.587843228026336E-5	1.896960807006584E-5	0.0	1.2140549164842138E-4	6.829058905223703E-5
44	7.967235389427653E-5	1.896960807006584E-5	3.793921614013168E-6	1.2899333487644771E-4	7.20845106662502E-5
45	8.726019712230287E-5	1.896960807006584E-5	1.1381764842039505E-5	1.3278725649046088E-4	7.20845106662502E-5
46	9.105411873631604E-5	1.896960807006584E-5	1.1381764842039505E-5	1.441690213325004E-4	7.587843228026336E-5
47	9.484804035032921E-5	1.896960807006584E-5	1.1381764842039505E-5	1.555507861745399E-4	8.34662755082897E-5
48	1.0243588357835555E-4	1.896960807006584E-5	1.1381764842039505E-5	1.555507861745399E-4	8.34662755082897E-5
49	1.0622980519236872E-4	1.896960807006584E-5	1.1381764842039505E-5	1.555507861745399E-4	8.34662755082897E-5
50	1.1002372680638187E-4	1.896960807006584E-5	1.1381764842039505E-5	1.555507861745399E-4	8.726019712230287E-5
51	1.1761157003440821E-4	1.896960807006584E-5	1.1381764842039505E-5	1.669325510165794E-4	8.726019712230287E-5
52	1.1761157003440821E-4	1.896960807006584E-5	1.1381764842039505E-5	1.7452039424460574E-4	8.726019712230287E-5
53	1.2140549164842138E-4	1.896960807006584E-5	1.1381764842039505E-5	1.8590215908664525E-4	8.726019712230287E-5
54	1.2140549164842138E-4	1.896960807006584E-5	1.1381764842039505E-5	1.8969608070065842E-4	8.726019712230287E-5
55	1.2140549164842138E-4	1.896960807006584E-5	1.1381764842039505E-5	1.9349000231467159E-4	9.105411873631604E-5
56	1.2140549164842138E-4	1.896960807006584E-5	1.1381764842039505E-5	1.9349000231467159E-4	9.484804035032921E-5
57	1.2519941326243455E-4	2.276352968407901E-5	1.1381764842039505E-5	1.9728392392868475E-4	9.484804035032921E-5
58	1.669325510165794E-4	2.655745129809218E-5	1.1381764842039505E-5	1.9728392392868475E-4	9.484804035032921E-5
59	1.9349000231467159E-4	2.655745129809218E-5	1.1381764842039505E-5	1.9728392392868475E-4	9.484804035032921E-5
60	2.0107784554269792E-4	2.655745129809218E-5	1.1381764842039505E-5	2.0107784554269792E-4	9.484804035032921E-5
61	2.0866568877072426E-4	2.655745129809218E-5	1.1381764842039505E-5	2.162535319987506E-4	9.484804035032921E-5
62	2.0866568877072426E-4	2.655745129809218E-5	1.1381764842039505E-5	2.2004745361276374E-4	9.484804035032921E-5
63	2.0866568877072426E-4	2.655745129809218E-5	1.1381764842039505E-5	2.238413752267769E-4	9.484804035032921E-5
64	2.0866568877072426E-4	2.655745129809218E-5	1.1381764842039505E-5	2.3142921845480325E-4	9.484804035032921E-5
65	2.1245961038473743E-4	3.0351372912105344E-5	1.5175686456052672E-5	2.3522314006881642E-4	9.864196196434238E-5
>>END_MODULE
