##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543819_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11395159
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.737479836832467	32.0	32.0	32.0	32.0	32.0
2	30.981301621153335	32.0	32.0	32.0	32.0	32.0
3	33.613627330693674	37.0	32.0	37.0	27.0	37.0
4	34.97360484395172	37.0	37.0	37.0	27.0	37.0
5	35.42816067770533	37.0	37.0	37.0	32.0	37.0
6	38.548171376985614	41.0	41.0	41.0	32.0	41.0
7	38.49340820957391	41.0	41.0	41.0	32.0	41.0
8	38.49560089508185	41.0	41.0	41.0	32.0	41.0
9	38.61356862155236	41.0	41.0	41.0	32.0	41.0
10-11	38.687292033397696	41.0	41.0	41.0	34.5	41.0
12-13	38.740276506892094	41.0	41.0	41.0	34.5	41.0
14-15	38.62514845119756	41.0	41.0	41.0	32.0	41.0
16-17	38.4261853652064	41.0	41.0	41.0	32.0	41.0
18-19	38.55883840673043	41.0	41.0	41.0	34.5	41.0
20-21	38.66286916224688	41.0	41.0	41.0	34.5	41.0
22-23	38.42567150664594	41.0	39.0	41.0	32.0	41.0
24-25	38.428649788914754	41.0	39.0	41.0	32.0	41.0
26-27	38.6287991242597	41.0	41.0	41.0	32.0	41.0
28-29	38.234861839137125	41.0	37.0	41.0	32.0	41.0
30-31	38.47708303148732	41.0	39.0	41.0	32.0	41.0
32-33	38.65105041535621	41.0	41.0	41.0	34.5	41.0
34-35	38.56820229537824	41.0	39.0	41.0	32.0	41.0
36-37	38.331305892124504	41.0	37.0	41.0	32.0	41.0
38-39	38.27850230040738	41.0	37.0	41.0	32.0	41.0
40-41	38.31431408852739	41.0	37.0	41.0	32.0	41.0
42-43	38.293945393409615	41.0	37.0	41.0	32.0	41.0
44-45	38.264718706226176	41.0	37.0	41.0	32.0	41.0
46-47	38.28387111593985	41.0	37.0	41.0	32.0	41.0
48-49	38.26332552692604	41.0	37.0	41.0	32.0	41.0
50-51	38.20649556321838	41.0	37.0	41.0	32.0	41.0
52-53	38.24169409965421	41.0	37.0	41.0	32.0	41.0
54-55	38.24602409690169	41.0	37.0	41.0	32.0	41.0
56-57	38.23890170593778	41.0	37.0	41.0	32.0	41.0
58-59	38.25875296412596	41.0	37.0	41.0	32.0	41.0
60-61	38.23045586618949	41.0	37.0	41.0	32.0	41.0
62-63	38.268799514577765	41.0	37.0	41.0	32.0	41.0
64-65	38.2559967728184	41.0	37.0	41.0	32.0	41.0
66-67	38.176135098104574	41.0	37.0	41.0	32.0	41.0
68-69	38.18864486652313	41.0	37.0	41.0	32.0	41.0
70-71	38.01141525919503	41.0	37.0	41.0	32.0	41.0
72-73	38.1246448198459	41.0	37.0	41.0	32.0	41.0
74-75	38.15641391835989	41.0	37.0	41.0	32.0	41.0
76	37.245325004815314	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	11205.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	25.0
13	122.0
14	481.0
15	1562.0
16	4700.0
17	10399.0
18	17041.0
19	23326.0
20	28762.0
21	33672.0
22	38910.0
23	44538.0
24	50893.0
25	58690.0
26	67372.0
27	77772.0
28	89845.0
29	104874.0
30	123249.0
31	145472.0
32	174089.0
33	210994.0
34	260432.0
35	335244.0
36	455010.0
37	672959.0
38	1199399.0
39	3011863.0
40	4142259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.94337018022771	19.41214918576141	13.898372162577955	31.746108471432922
2	22.204808135260922	27.740578954030592	29.15346865653471	20.901144254173772
3	21.265985438802723	28.688555839210174	27.86775139815217	22.177707323834937
4	25.002088487387862	33.067558855594996	19.477931737778746	22.452420919238392
5	24.44875315891707	35.17498959911621	20.152801632657653	20.22345560930907
6	19.866906374337155	37.37254110334749	20.907905978513803	21.85264654380155
7	20.00035839992354	21.487252514092013	34.023642623975846	24.4887464620086
8	20.987515179540228	23.78486374957132	26.491916894828275	28.735704176060178
9	21.90368459389915	23.421087861933408	27.748142874862392	26.927084669305053
10-11	23.75847175819997	31.00090540522616	21.34689370988891	23.893729126684963
12-13	23.480529702253854	25.203329162985494	25.218060439078993	26.09808069568166
14-15	22.65278951951072	28.062264447953496	25.704872918726597	23.580073113809192
16-17	22.982428770056067	28.079243235759897	25.139214935984672	23.79911305819936
18-19	22.77359120646824	28.3113583909183	25.30480133169526	23.6102490709182
20-21	22.85130261397721	28.251593720087758	25.312686078220747	23.584417587714285
22-23	23.072471788587624	27.857052660937086	25.30265846649823	23.76781708397706
24-25	22.956943922784152	27.772462711580985	25.42376372953634	23.84682963609852
26-27	22.675051985854594	28.18797051958535	25.339102204302133	23.797875290257924
28-29	23.390529436429336	27.471426765523827	25.1813029849974	23.95674081304944
30-31	23.006169329166386	27.697198153075586	25.523353673444877	23.773278844313147
32-33	22.783984131789232	28.055796694523984	25.553325523461922	23.606893650224862
34-35	23.112143115973225	27.9579773704431	25.0207892754753	23.909090238108377
36-37	22.779609982004455	27.70052018275157	25.53490370287835	23.984966132365628
38-39	22.500438314919712	27.99221886656815	25.74882423945221	23.758518579059928
40-41	22.986367605272147	27.670471072585652	25.308905189181786	24.034256132960422
42-43	22.588786514452778	27.657277094149325	25.601353175131493	24.152583216266407
44-45	22.686121534247818	27.613382513285966	25.53130887330857	24.16918707915764
46-47	22.98741931647071	27.44065985985211	25.28213704751103	24.289783776166153
48-49	22.79865359755456	27.429675261273527	25.626874600273304	24.144796540898607
50-51	22.698579124399288	27.554447700830654	25.711728001563465	24.035245173206597
52-53	22.866307836990813	27.451737737218235	25.34815832493885	24.3337961008521
54-55	22.807963126575554	27.1106171028547	25.825849231545323	24.255570539024422
56-57	22.551954548660422	27.65674999767619	25.5266600889268	24.264635364736588
58-59	22.729591168835704	27.712680209245082	25.497246211529852	24.06048241038937
60-61	22.761730789323188	27.344017754906037	25.866100533953517	24.02815092181726
62-63	22.578235777248178	27.729052661303204	25.657700800935608	24.03501076051301
64-65	23.051771603431206	27.40809006149379	25.528278039162178	24.01186029591283
66-67	22.898212645334606	27.38699769148501	25.80783211224567	23.906957550934713
68-69	22.685479978227157	27.47762156828928	25.974594158517494	23.862304294966073
70-71	23.004309057353204	27.59723878863449	25.65692857852345	23.741523575488863
72-73	22.70186088280087	27.430397739480327	25.711465357316026	24.156276020402778
74-75	22.667220188185578	24.17866612386911	27.486555266574342	25.667558421370966
76	23.872719485939555	0.0	38.61602868106717	37.51125183299327
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20688.0
1	11674.0
2	2651.0
3	2805.0
4	2968.5
5	2758.0
6	3001.5
7	4069.0
8	4682.0
9	5042.0
10	6556.0
11	15163.0
12	22623.0
13	28955.0
14	25608.5
15	13144.5
16	8034.0
17	5952.0
18	6425.5
19	7809.0
20	10757.0
21	13650.0
22	17100.0
23	23571.0
24	33248.5
25	42390.0
26	49901.0
27	63464.0
28	81895.0
29	94038.0
30	115925.5
31	153041.5
32	186572.5
33	206757.0
34	244286.0
35	292968.5
36	322059.5
37	338712.0
38	351658.0
39	382500.0
40	415260.0
41	435536.5
42	450270.5
43	483850.5
44	538545.0
45	573383.0
46	588527.5
47	629732.5
48	658747.5
49	648679.5
50	637228.5
51	627151.0
52	619202.5
53	597570.0
54	571923.5
55	527653.5
56	462944.0
57	396767.0
58	350793.5
59	311148.5
60	259827.0
61	214555.5
62	182073.5
63	152996.0
64	118606.0
65	91305.0
66	67393.5
67	55759.0
68	47489.0
69	36838.5
70	28685.5
71	23519.0
72	20907.0
73	19448.0
74	15568.0
75	11070.5
76	8716.5
77	6438.5
78	5127.0
79	4418.5
80	4077.5
81	3588.0
82	3053.5
83	2721.0
84	2373.0
85	1806.0
86	1388.5
87	1250.5
88	1161.0
89	788.5
90	391.0
91	255.0
92	232.0
93	220.5
94	188.0
95	175.5
96	184.0
97	172.5
98	142.0
99	104.5
100	86.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.2359722229413386
2	0.2366619017777637
3	0.09833122995475535
4	0.09935798175347971
5	0.10101658081295752
6	0.09910348771789844
7	0.09853307005194048
8	0.10017411779861957
9	0.10034963092660663
10-11	0.09921757125109004
12-13	0.0984321500033479
14-15	0.09884021802591784
16-17	0.09936675740987906
18-19	0.10068749369798176
20-21	0.09949400442766969
22-23	0.09869541969532852
24-25	0.10442592332410633
26-27	0.10121403308194295
28-29	0.10413193883472796
30-31	0.11022224437587927
32-33	0.11767716448713
34-35	0.1254129056031601
36-37	0.002864582990088631
38-39	0.003739268661444199
40-41	0.0010327165481357093
42-43	0.0021932398107748293
44-45	0.0014858922008947357
46-47	1.5389729510114128E-4
48-49	0.004173851056235012
50-51	0.004139911589790283
52-53	0.0016372054768572205
54-55	0.009770298477555018
56-57	0.004136430490435286
58-59	0.003878611108148283
60-61	0.001040825635817668
62-63	3.1777628628889334E-4
64-65	6.670090941168384E-4
66-67	0.005381204386057419
68-69	0.003930856586769941
70-71	0.003895857652896014
72-73	0.0013803884297266737
74-75	2.522346082634723E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	14543.0
36	502.0
37	560.0
38	647.0
39	725.0
40	846.0
41	930.0
42	1082.0
43	1148.0
44	1076.0
45	1234.0
46	1292.0
47	1383.0
48	1586.0
49	1686.0
50	1884.0
51	2084.0
52	2258.0
53	2518.0
54	2659.0
55	2722.0
56	2856.0
57	3044.0
58	3254.0
59	3504.0
60	3965.0
61	4236.0
62	4421.0
63	4754.0
64	5148.0
65	5569.0
66	6331.0
67	6247.0
68	6434.0
69	6853.0
70	7930.0
71	18539.0
72	59971.0
73	272124.0
74	841044.0
75	2837862.0
76	7247708.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.1018029815363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.60884124477583	26.12499610773138
2	10.831966208023736	7.387791550573686
3	4.037645127533439	4.130729359455161
4	2.0049630181288727	2.734914153179888
5	1.2716765242651946	2.168323114336827
6	0.8258354214049258	1.6897486101554875
7	0.6262702861550025	1.4949862138153782
8	0.45366806756245914	1.2376719247223424
9	0.35596185713630374	1.0925047008903601
>10	2.4303248608733576	16.2015954403133
>50	0.2771651317331515	6.5783555272665355
>100	0.23813121435010492	16.461485035290025
>500	0.026579672418445686	6.1976257148979075
>1k	0.010810022086406409	5.808464793098638
>5k	8.067177632423667E-5	0.19386670648709634
>10k+	8.067177632423667E-5	0.49694104778592485
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31516	0.2765735870820232	No Hit
CCCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11470	0.10065677890058401	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.775656399353445E-6	0.0
2	0.0	0.0	0.0	8.775656399353445E-6	0.0
3	0.0	0.0	0.0	8.775656399353445E-6	0.0
4	0.0	0.0	0.0	8.775656399353445E-6	0.0
5	0.0	0.0	0.0	8.775656399353445E-6	0.0
6	0.0	0.0	0.0	1.755131279870689E-5	0.0
7	0.0	8.775656399353445E-6	0.0	4.387828199676722E-5	0.0
8	0.0	8.775656399353445E-6	0.0	4.387828199676722E-5	0.0
9	0.0	8.775656399353445E-6	0.0	7.898090759418101E-5	0.0
10	0.0	8.775656399353445E-6	0.0	7.898090759418101E-5	0.0
11	0.0	8.775656399353445E-6	0.0	7.898090759418101E-5	0.0
12	8.775656399353445E-6	8.775656399353445E-6	0.0	9.653222039288789E-5	0.0
13	8.775656399353445E-6	8.775656399353445E-6	0.0	9.653222039288789E-5	0.0
14	1.755131279870689E-5	8.775656399353445E-6	0.0	9.653222039288789E-5	0.0
15	1.755131279870689E-5	8.775656399353445E-6	0.0	9.653222039288789E-5	0.0
16	2.6326969198060334E-5	8.775656399353445E-6	0.0	9.653222039288789E-5	0.0
17	2.6326969198060334E-5	8.775656399353445E-6	0.0	9.653222039288789E-5	0.0
18	2.6326969198060334E-5	8.775656399353445E-6	0.0	9.653222039288789E-5	0.0
19	2.6326969198060334E-5	8.775656399353445E-6	0.0	9.653222039288789E-5	0.0
20	2.6326969198060334E-5	8.775656399353445E-6	0.0	1.0530787679224134E-4	0.0
21	2.6326969198060334E-5	8.775656399353445E-6	0.0	1.0530787679224134E-4	0.0
22	4.387828199676722E-5	8.775656399353445E-6	0.0	1.0530787679224134E-4	0.0
23	5.265393839612067E-5	8.775656399353445E-6	0.0	1.0530787679224134E-4	0.0
24	6.142959479547412E-5	8.775656399353445E-6	0.0	1.0530787679224134E-4	0.0
25	7.898090759418101E-5	8.775656399353445E-6	0.0	1.0530787679224134E-4	0.0
26	8.775656399353444E-5	8.775656399353445E-6	0.0	1.0530787679224134E-4	0.0
27	9.653222039288789E-5	8.775656399353445E-6	0.0	1.0530787679224134E-4	0.0
28	1.2285918959094823E-4	8.775656399353445E-6	0.0	1.0530787679224134E-4	0.0
29	1.4041050238965512E-4	8.775656399353445E-6	0.0	1.0530787679224134E-4	0.0
30	1.4918615878900857E-4	8.775656399353445E-6	0.0	1.0530787679224134E-4	0.0
31	1.7551312798706889E-4	8.775656399353445E-6	0.0	1.0530787679224134E-4	0.0
32	2.0184009718512923E-4	8.775656399353445E-6	0.0	1.0530787679224134E-4	0.0
33	2.3694272278254301E-4	8.775656399353445E-6	0.0	1.0530787679224134E-4	0.0
34	2.544940355812499E-4	8.775656399353445E-6	0.0	1.3163484599030168E-4	0.0
35	2.8959666117866367E-4	8.775656399353445E-6	0.0	1.4041050238965512E-4	0.0
36	3.0714797397737056E-4	8.775656399353445E-6	0.0	1.5796181518836202E-4	0.0
37	3.4225059957478435E-4	8.775656399353445E-6	0.0	1.5796181518836202E-4	0.0
38	3.7735322517219814E-4	1.755131279870689E-5	0.0	1.5796181518836202E-4	0.0
39	3.8612888157155156E-4	1.755131279870689E-5	0.0	1.5796181518836202E-4	0.0
40	3.9490453797090503E-4	1.755131279870689E-5	0.0	1.6673747158771544E-4	0.0
41	4.124558507696119E-4	1.755131279870689E-5	0.0	1.6673747158771544E-4	0.0
42	4.3000716356831877E-4	1.755131279870689E-5	0.0	1.6673747158771544E-4	0.0
43	4.3878281996767224E-4	1.755131279870689E-5	0.0	1.7551312798706889E-4	0.0
44	4.5633413276637913E-4	1.755131279870689E-5	0.0	1.7551312798706889E-4	0.0
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46	4.7388544556508603E-4	1.755131279870689E-5	0.0	1.7551312798706889E-4	0.0
47	5.002124147631463E-4	1.755131279870689E-5	0.0	1.7551312798706889E-4	0.0
48	5.177637275618533E-4	1.755131279870689E-5	0.0	1.9306444078577578E-4	0.0
49	5.704176659579739E-4	1.755131279870689E-5	0.0	2.0184009718512923E-4	0.0
50	5.967446351560343E-4	1.755131279870689E-5	0.0	2.1061575358448267E-4	0.0
51	6.055202915553877E-4	1.755131279870689E-5	0.0	2.1939140998383612E-4	0.0
52	6.142959479547411E-4	1.755131279870689E-5	0.0	2.1939140998383612E-4	0.0
53	6.142959479547411E-4	1.755131279870689E-5	0.0	2.1939140998383612E-4	0.0
54	6.230716043540945E-4	1.755131279870689E-5	0.0	2.1939140998383612E-4	0.0
55	6.581742299515083E-4	1.755131279870689E-5	0.0	2.2816706638318957E-4	0.0
56	6.757255427502153E-4	1.755131279870689E-5	0.0	2.2816706638318957E-4	0.0
57	7.108281683476291E-4	1.755131279870689E-5	0.0	2.4571837918189646E-4	0.0
58	7.459307939450429E-4	1.755131279870689E-5	0.0	2.544940355812499E-4	0.0
59	8.249117015392239E-4	1.755131279870689E-5	0.0	2.6326969198060335E-4	0.0
60	8.687899835359911E-4	1.755131279870689E-5	0.0	2.6326969198060335E-4	0.0
61	8.863412963346979E-4	1.755131279870689E-5	0.0	2.6326969198060335E-4	0.0
62	8.863412963346979E-4	1.755131279870689E-5	0.0	2.720453483799568E-4	0.0
63	8.951169527340513E-4	1.755131279870689E-5	0.0	2.8082100477931025E-4	0.0
64	8.951169527340513E-4	1.755131279870689E-5	0.0	2.8082100477931025E-4	0.0
65	9.038926091334048E-4	2.6326969198060334E-5	0.0	2.8959666117866367E-4	0.0
>>END_MODULE
