##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8543819_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11395159
Sequences flagged as poor quality	0
Sequence length	35-76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52657747030998	32.0	32.0	32.0	32.0	32.0
2	31.722632654796655	32.0	32.0	32.0	32.0	32.0
3	35.89155035046023	37.0	37.0	37.0	32.0	37.0
4	36.41956536104498	37.0	37.0	37.0	37.0	37.0
5	36.584314883188554	37.0	37.0	37.0	37.0	37.0
6	40.126125839929045	41.0	41.0	41.0	37.0	41.0
7	40.157268187306556	41.0	41.0	41.0	37.0	41.0
8	40.13475801434627	41.0	41.0	41.0	37.0	41.0
9	40.064682116326765	41.0	41.0	41.0	37.0	41.0
10-11	39.811776342918954	41.0	41.0	41.0	37.0	41.0
12-13	39.670233122679555	41.0	41.0	41.0	37.0	41.0
14-15	39.66133574792594	41.0	41.0	41.0	37.0	41.0
16-17	39.67465508818262	41.0	41.0	41.0	37.0	41.0
18-19	39.637291414713914	41.0	41.0	41.0	37.0	41.0
20-21	39.631456085869445	41.0	41.0	41.0	37.0	41.0
22-23	39.61421595784666	41.0	41.0	41.0	37.0	41.0
24-25	39.5812450708235	41.0	41.0	41.0	37.0	41.0
26-27	39.469471378152775	41.0	41.0	41.0	37.0	41.0
28-29	39.47351673636146	41.0	41.0	41.0	37.0	41.0
30-31	39.52447179543523	41.0	41.0	41.0	37.0	41.0
32-33	39.60074993249326	41.0	41.0	41.0	37.0	41.0
34-35	39.616615661089064	41.0	41.0	41.0	37.0	41.0
36-37	39.76975416921016	41.0	41.0	41.0	37.0	41.0
38-39	39.85967489157344	41.0	41.0	41.0	37.0	41.0
40-41	39.9373076238697	41.0	41.0	41.0	37.0	41.0
42-43	39.950277558065594	41.0	41.0	41.0	37.0	41.0
44-45	39.96654079889781	41.0	41.0	41.0	37.0	41.0
46-47	39.888625111879264	41.0	41.0	41.0	37.0	41.0
48-49	39.960439482741016	41.0	41.0	41.0	37.0	41.0
50-51	39.946717375711025	41.0	41.0	41.0	37.0	41.0
52-53	39.89264277815283	41.0	41.0	41.0	37.0	41.0
54-55	39.93128019711692	41.0	41.0	41.0	37.0	41.0
56-57	39.928245181727036	41.0	41.0	41.0	37.0	41.0
58-59	39.857289100891634	41.0	41.0	41.0	37.0	41.0
60-61	39.89101550878612	41.0	41.0	41.0	37.0	41.0
62-63	39.876129874978304	41.0	41.0	41.0	37.0	41.0
64-65	39.820404045781	41.0	41.0	41.0	37.0	41.0
66-67	39.82295295061951	41.0	41.0	41.0	37.0	41.0
68-69	39.81449686126126	41.0	41.0	41.0	37.0	41.0
70-71	39.7969416010964	41.0	41.0	41.0	37.0	41.0
72-73	39.74738384937906	41.0	41.0	41.0	37.0	41.0
74-75	39.72885285963943	41.0	41.0	41.0	37.0	41.0
76	39.228289710747866	41.0	41.0	41.0	37.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	11529.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	5.0
16	6.0
17	14.0
18	27.0
19	84.0
20	146.0
21	366.0
22	877.0
23	1586.0
24	2671.0
25	4656.0
26	7689.0
27	13120.0
28	19892.0
29	30321.0
30	44164.0
31	61968.0
32	84851.0
33	115242.0
34	155597.0
35	209900.0
36	291051.0
37	429364.0
38	739338.0
39	2137350.0
40	7033344.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.477534062819295	10.084978390116358	11.954100936567027	36.48338661049732
2	23.149652323949173	14.861874470970744	34.46919522509069	27.519277979989397
3	22.627114549576895	19.370798242739795	27.690631195848773	30.311456011834537
4	27.414386788412454	26.435395359726815	20.93264052971452	25.217577322146216
5	26.64744901230978	29.610546020908973	24.461546975788917	19.28045799099233
6	22.158848435197072	31.951562624356466	26.73706380913043	19.152525131316033
7	18.755713247883143	22.006908165497297	42.12050110553488	17.11687748108468
8	21.366121317850126	21.888381365439635	33.62418447378822	23.121312842922023
9	21.616698715611804	21.161141042005056	35.14125107720472	22.08090916517842
10-11	24.521035908581005	29.306280158438035	25.900402595657095	20.272281337323857
12-13	24.21985956872689	23.41682540779916	29.941423061673373	22.421891961800576
14-15	23.28454663491163	25.373779993967638	30.045074047962768	21.296599323157967
16-17	23.713889542729312	25.591200461119644	29.40228512140254	21.292624874748505
18-19	23.704360885267782	25.81494881816686	29.201114161397918	21.279576135167442
20-21	23.24234009713949	25.77929447812341	29.529688684540872	21.44867674019623
22-23	23.72302876602203	25.47844214123554	28.99057340144953	21.8079556912929
24-25	23.633427867033088	25.663081788656015	28.8963284623156	21.80716188199529
26-27	23.385627227363504	26.0040352538032	28.90891985600792	21.70141766282538
28-29	23.86951489822596	25.670601812824618	28.891889011605652	21.567994277343768
30-31	23.58540806261055	25.84662374696426	28.682359571810405	21.885608618614786
32-33	23.458820866686718	25.641515300800094	28.905158524834405	21.994505307678782
34-35	23.50851961324178	25.830985311563094	28.8085159140476	21.85197916114753
36-37	24.102704700041873	25.460085474479143	28.50569925057151	21.93151057490748
38-39	23.458933867860164	25.87181858100598	28.903389654072516	21.765857897061345
40-41	23.90442890289231	25.394876262546028	28.902843970442778	21.797850864118885
42-43	23.43886936151335	25.11580571987009	29.358012005581585	22.087312913034978
44-45	23.6848641737327	25.279959618148478	29.21503160085624	21.820144607262577
46-47	23.634388443415162	25.463438219948376	29.108869627679645	21.793303708956813
48-49	23.653323599717755	25.271865592010528	29.09525799835655	21.979552809915162
50-51	23.413171819106367	25.62710419860379	29.005186022991385	21.954537959298456
52-53	23.68322455388558	25.3164676740038	28.90365218943569	22.096655582674927
54-55	23.548592549442564	25.60794541785192	28.578772253913275	22.264689778792242
56-57	23.926198644280593	25.255530531219545	28.954052015132074	21.864218809367788
58-59	23.778763689761863	25.20712739228827	28.941430278383816	22.07267863956605
60-61	23.198963010842675	25.271221301622326	29.18356112329731	22.346254564237686
62-63	23.206453324004485	25.555397691348524	29.225060791328687	22.0130881933183
64-65	23.652580144252564	25.464119050750078	28.822242181592884	22.061058623404474
66-67	23.82006605667758	25.353332301911657	28.671274931372803	22.15532671003796
68-69	23.47166200995096	25.789257649386464	28.784841762924955	21.954238577737613
70-71	23.792879077663855	25.490665152532316	28.616997458104997	22.099458311698832
72-73	23.731672780375632	25.108625772387384	28.754862091975237	22.40483935526175
74-75	23.78403849078702	21.98760835999362	30.39798763538606	23.830365513833307
76	24.568096550621078	0.0	42.04401784628435	33.387885603094574
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	97744.0
1	72382.0
2	30171.5
3	12382.5
4	11442.5
5	12794.5
6	17782.0
7	25322.5
8	29227.0
9	28853.0
10	31404.5
11	28182.0
12	22035.5
13	19065.0
14	10625.0
15	4481.5
16	3725.5
17	3792.0
18	4337.0
19	6222.0
20	8864.5
21	10809.5
22	13964.5
23	19051.0
24	25363.5
25	31516.5
26	43254.0
27	63495.0
28	84963.0
29	103226.0
30	128495.0
31	156954.0
32	184027.5
33	208922.5
34	243965.0
35	293009.5
36	335706.5
37	357203.0
38	364831.5
39	390874.5
40	427418.0
41	448467.0
42	458750.0
43	490671.5
44	532820.5
45	559535.5
46	570135.5
47	598221.5
48	622764.5
49	618560.0
50	611536.0
51	609951.0
52	615552.0
53	583418.0
54	549638.5
55	515455.5
56	455298.0
57	392944.0
58	348987.5
59	310511.0
60	259549.0
61	208817.5
62	172661.5
63	146476.5
64	114918.5
65	89601.0
66	65710.0
67	52315.5
68	40124.0
69	27484.0
70	21955.5
71	18973.0
72	15910.0
73	12820.0
74	9705.0
75	6849.5
76	5633.5
77	4300.5
78	3603.5
79	3574.0
80	3016.0
81	2546.0
82	2181.5
83	1684.5
84	1553.5
85	1386.0
86	1322.5
87	1355.0
88	914.0
89	394.0
90	180.5
91	73.0
92	54.0
93	43.0
94	28.0
95	19.0
96	14.0
97	10.0
98	5.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9007421484860368
2	0.15962041424783982
3	0.10117454262814586
4	0.10902875510556718
5	0.10117454262814586
6	0.10138515838173034
7	0.10117454262814586
8	0.1014290366637271
9	0.10117454262814586
10-11	0.10117454262814586
12-13	0.10165281590191064
14-15	0.10142464883552743
16-17	0.1014685271175242
18-19	0.10118331828454523
20-21	0.10117454262814586
22-23	0.10155628368151776
24-25	0.10303059395660912
26-27	0.11169216682277096
28-29	0.1143643541963741
30-31	0.11453547949616148
32-33	0.11961219672318746
34-35	0.12550943782355298
36-37	5.492058812480681E-4
38-39	0.0013973175282366893
40-41	3.47176566794662E-4
42-43	8.790724625144821E-6
44-45	0.0
46-47	8.794268810193472E-6
48-49	6.509364358371296E-4
50-51	5.455422161684634E-4
52-53	2.0685341451611E-4
54-55	1.4089786371099348E-4
56-57	0.001845770744524889
58-59	0.0021905519247634887
60-61	4.4103174083387196E-6
62-63	0.0029438674650564696
64-65	0.0
66-67	9.727517639637301E-4
68-69	0.0013721855423255722
70-71	0.0
72-73	1.7801299227824144E-5
74-75	6.189497660369884E-4
76	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35	14843.0
36	497.0
37	555.0
38	637.0
39	712.0
40	838.0
41	916.0
42	1069.0
43	1136.0
44	1060.0
45	1216.0
46	1276.0
47	1378.0
48	1577.0
49	1674.0
50	1870.0
51	2081.0
52	2244.0
53	2508.0
54	2657.0
55	2714.0
56	2858.0
57	3041.0
58	3258.0
59	3508.0
60	3967.0
61	4233.0
62	4402.0
63	4752.0
64	5153.0
65	5404.0
66	5997.0
67	6066.0
68	6427.0
69	6769.0
70	7464.0
71	18751.0
72	49032.0
73	212597.0
74	831160.0
75	2626693.0
76	7540169.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.133408359189755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.85895124519406	20.934961708893457
2	12.939760599151803	7.539586592104867
3	4.947172798432983	4.323840160806709
4	2.355327478958893	2.744748690565214
5	1.4824212155359473	2.1593991316267602
6	1.0441832676835667	1.8252370523555095
7	0.7421045945727406	1.5134025339243022
8	0.5697616280006497	1.3279278540751753
9	0.4473451126928647	1.172941906101209
>10	2.9640196157976586	16.850030057594697
>50	0.33072539233876	6.728428348042296
>100	0.27166285194643286	16.087949458442555
>500	0.030750984533319165	6.174866663514549
>1k	0.015213512473065483	7.545532500747195
>5k	2.8406969410329336E-4	0.5799671660618682
>10k+	3.156329934481037E-4	2.491180175143647
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	78667	0.6903545619679374	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39136	0.3434440888450964	No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31153	0.27338802380905786	No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27123	0.23802212851966345	No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24834	0.21793465102154347	No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19311	0.16946670072791437	No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17042	0.1495547363577814	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	11529	0.10117454262814586	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.775656399353445E-6
2	0.0	8.775656399353445E-6	0.0	0.0	1.755131279870689E-5
3	0.0	8.775656399353445E-6	0.0	0.0	1.755131279870689E-5
4	8.775656399353445E-6	8.775656399353445E-6	0.0	8.775656399353445E-6	1.755131279870689E-5
5	8.775656399353445E-6	8.775656399353445E-6	0.0	8.775656399353445E-6	1.755131279870689E-5
6	8.775656399353445E-6	8.775656399353445E-6	0.0	8.775656399353445E-6	1.755131279870689E-5
7	8.775656399353445E-6	8.775656399353445E-6	0.0	8.775656399353445E-6	1.755131279870689E-5
8	8.775656399353445E-6	8.775656399353445E-6	0.0	8.775656399353445E-6	1.755131279870689E-5
9	8.775656399353445E-6	8.775656399353445E-6	8.775656399353445E-6	2.6326969198060334E-5	1.755131279870689E-5
10	8.775656399353445E-6	8.775656399353445E-6	8.775656399353445E-6	2.6326969198060334E-5	1.755131279870689E-5
11	8.775656399353445E-6	8.775656399353445E-6	8.775656399353445E-6	2.6326969198060334E-5	2.6326969198060334E-5
12	8.775656399353445E-6	8.775656399353445E-6	8.775656399353445E-6	2.6326969198060334E-5	2.6326969198060334E-5
13	8.775656399353445E-6	8.775656399353445E-6	8.775656399353445E-6	2.6326969198060334E-5	2.6326969198060334E-5
14	8.775656399353445E-6	8.775656399353445E-6	8.775656399353445E-6	3.510262559741378E-5	2.6326969198060334E-5
15	8.775656399353445E-6	8.775656399353445E-6	8.775656399353445E-6	3.510262559741378E-5	3.510262559741378E-5
16	8.775656399353445E-6	8.775656399353445E-6	1.755131279870689E-5	3.510262559741378E-5	3.510262559741378E-5
17	8.775656399353445E-6	8.775656399353445E-6	1.755131279870689E-5	3.510262559741378E-5	3.510262559741378E-5
18	8.775656399353445E-6	8.775656399353445E-6	1.755131279870689E-5	3.510262559741378E-5	5.265393839612067E-5
19	1.755131279870689E-5	8.775656399353445E-6	1.755131279870689E-5	3.510262559741378E-5	5.265393839612067E-5
20	1.755131279870689E-5	8.775656399353445E-6	1.755131279870689E-5	3.510262559741378E-5	5.265393839612067E-5
21	1.755131279870689E-5	8.775656399353445E-6	1.755131279870689E-5	3.510262559741378E-5	5.265393839612067E-5
22	1.755131279870689E-5	8.775656399353445E-6	1.755131279870689E-5	3.510262559741378E-5	5.265393839612067E-5
23	1.755131279870689E-5	8.775656399353445E-6	1.755131279870689E-5	3.510262559741378E-5	5.265393839612067E-5
24	1.755131279870689E-5	8.775656399353445E-6	1.755131279870689E-5	4.387828199676722E-5	5.265393839612067E-5
25	2.6326969198060334E-5	8.775656399353445E-6	1.755131279870689E-5	4.387828199676722E-5	5.265393839612067E-5
26	2.6326969198060334E-5	8.775656399353445E-6	1.755131279870689E-5	4.387828199676722E-5	5.265393839612067E-5
27	2.6326969198060334E-5	8.775656399353445E-6	3.510262559741378E-5	4.387828199676722E-5	5.265393839612067E-5
28	2.6326969198060334E-5	8.775656399353445E-6	3.510262559741378E-5	4.387828199676722E-5	5.265393839612067E-5
29	3.510262559741378E-5	8.775656399353445E-6	3.510262559741378E-5	4.387828199676722E-5	6.142959479547412E-5
30	3.510262559741378E-5	8.775656399353445E-6	3.510262559741378E-5	5.265393839612067E-5	6.142959479547412E-5
31	3.510262559741378E-5	8.775656399353445E-6	3.510262559741378E-5	6.142959479547412E-5	6.142959479547412E-5
32	3.510262559741378E-5	1.755131279870689E-5	3.510262559741378E-5	6.142959479547412E-5	6.142959479547412E-5
33	4.387828199676722E-5	1.755131279870689E-5	3.510262559741378E-5	6.142959479547412E-5	6.142959479547412E-5
34	4.387828199676722E-5	1.755131279870689E-5	3.510262559741378E-5	7.020525119482756E-5	6.142959479547412E-5
35	5.265393839612067E-5	1.755131279870689E-5	3.510262559741378E-5	7.898090759418101E-5	6.142959479547412E-5
36	5.265393839612067E-5	1.755131279870689E-5	3.510262559741378E-5	7.898090759418101E-5	6.142959479547412E-5
37	6.142959479547412E-5	1.755131279870689E-5	3.510262559741378E-5	7.898090759418101E-5	6.142959479547412E-5
38	7.020525119482756E-5	1.755131279870689E-5	3.510262559741378E-5	9.653222039288789E-5	7.020525119482756E-5
39	7.020525119482756E-5	1.755131279870689E-5	3.510262559741378E-5	9.653222039288789E-5	7.020525119482756E-5
40	7.020525119482756E-5	1.755131279870689E-5	3.510262559741378E-5	1.0530787679224134E-4	7.020525119482756E-5
41	7.020525119482756E-5	1.755131279870689E-5	3.510262559741378E-5	1.1408353319159478E-4	7.898090759418101E-5
42	7.020525119482756E-5	1.755131279870689E-5	3.510262559741378E-5	1.3163484599030168E-4	7.898090759418101E-5
43	7.020525119482756E-5	2.6326969198060334E-5	3.510262559741378E-5	1.3163484599030168E-4	9.653222039288789E-5
44	7.020525119482756E-5	2.6326969198060334E-5	3.510262559741378E-5	1.3163484599030168E-4	9.653222039288789E-5
45	7.898090759418101E-5	2.6326969198060334E-5	3.510262559741378E-5	1.3163484599030168E-4	9.653222039288789E-5
46	9.653222039288789E-5	2.6326969198060334E-5	3.510262559741378E-5	1.3163484599030168E-4	9.653222039288789E-5
47	1.0530787679224134E-4	2.6326969198060334E-5	3.510262559741378E-5	1.3163484599030168E-4	9.653222039288789E-5
48	1.1408353319159478E-4	2.6326969198060334E-5	3.510262559741378E-5	1.3163484599030168E-4	9.653222039288789E-5
49	1.2285918959094823E-4	2.6326969198060334E-5	3.510262559741378E-5	1.3163484599030168E-4	9.653222039288789E-5
50	1.4918615878900857E-4	2.6326969198060334E-5	3.510262559741378E-5	1.3163484599030168E-4	1.2285918959094823E-4
51	1.4918615878900857E-4	2.6326969198060334E-5	3.510262559741378E-5	1.3163484599030168E-4	1.2285918959094823E-4
52	1.4918615878900857E-4	2.6326969198060334E-5	3.510262559741378E-5	1.3163484599030168E-4	1.3163484599030168E-4
53	1.6673747158771544E-4	2.6326969198060334E-5	3.510262559741378E-5	1.3163484599030168E-4	1.3163484599030168E-4
54	1.7551312798706889E-4	2.6326969198060334E-5	3.510262559741378E-5	1.3163484599030168E-4	1.3163484599030168E-4
55	1.8428878438642233E-4	2.6326969198060334E-5	3.510262559741378E-5	1.4918615878900857E-4	1.3163484599030168E-4
56	1.8428878438642233E-4	3.510262559741378E-5	3.510262559741378E-5	1.4918615878900857E-4	1.3163484599030168E-4
57	1.8428878438642233E-4	3.510262559741378E-5	3.510262559741378E-5	1.4918615878900857E-4	1.4041050238965512E-4
58	2.3694272278254301E-4	3.510262559741378E-5	3.510262559741378E-5	1.4918615878900857E-4	1.4041050238965512E-4
59	2.3694272278254301E-4	3.510262559741378E-5	3.510262559741378E-5	1.4918615878900857E-4	1.4041050238965512E-4
60	2.8082100477931025E-4	3.510262559741378E-5	3.510262559741378E-5	1.4918615878900857E-4	1.4041050238965512E-4
61	2.8082100477931025E-4	3.510262559741378E-5	4.387828199676722E-5	1.4918615878900857E-4	1.4041050238965512E-4
62	2.8082100477931025E-4	3.510262559741378E-5	4.387828199676722E-5	1.4918615878900857E-4	1.4041050238965512E-4
63	2.8959666117866367E-4	3.510262559741378E-5	4.387828199676722E-5	1.5796181518836202E-4	1.4041050238965512E-4
64	2.8959666117866367E-4	3.510262559741378E-5	4.387828199676722E-5	1.5796181518836202E-4	1.4041050238965512E-4
65	2.9837231757801714E-4	4.387828199676722E-5	5.265393839612067E-5	1.6673747158771544E-4	1.5796181518836202E-4
>>END_MODULE
