##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8450747_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33031859
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44499000192511	33.0	33.0	33.0	33.0	33.0
2	28.077281875052808	33.0	33.0	33.0	2.0	33.0
3	29.850916353209186	33.0	33.0	33.0	15.0	33.0
4	35.7731983234731	37.0	37.0	37.0	33.0	37.0
5	36.49627733637395	37.0	37.0	37.0	37.0	37.0
6	36.52686750085728	37.0	37.0	37.0	37.0	37.0
7	36.546934037227516	37.0	37.0	37.0	37.0	37.0
8	36.54893371275289	37.0	37.0	37.0	37.0	37.0
9	36.55288895487232	37.0	37.0	37.0	37.0	37.0
10	36.500524781242255	37.0	37.0	37.0	37.0	37.0
11	36.52488771522063	37.0	37.0	37.0	37.0	37.0
12	36.51820189714421	37.0	37.0	37.0	37.0	37.0
13	36.50789142687973	37.0	37.0	37.0	37.0	37.0
14	38.51053799303273	40.0	37.0	40.0	37.0	40.0
15	38.509744486376015	40.0	37.0	40.0	37.0	40.0
16	38.50408649419338	40.0	37.0	40.0	37.0	40.0
17	38.46201880433069	40.0	37.0	40.0	37.0	40.0
18	38.45230978371517	40.0	37.0	40.0	37.0	40.0
19	38.44338309872296	40.0	37.0	40.0	37.0	40.0
20	38.363985660025975	40.0	37.0	40.0	37.0	40.0
21	38.32593100497311	40.0	37.0	40.0	37.0	40.0
22	38.3035985047042	40.0	37.0	40.0	37.0	40.0
23	38.26763068345623	40.0	37.0	40.0	37.0	40.0
24	38.23675546084161	40.0	37.0	40.0	37.0	40.0
25	38.22368071987714	40.0	37.0	40.0	37.0	40.0
26	38.14174479250472	40.0	37.0	40.0	37.0	40.0
27	38.10053924001068	40.0	37.0	40.0	37.0	40.0
28	38.02072562734056	40.0	37.0	40.0	33.0	40.0
29	38.00678451067498	40.0	37.0	40.0	33.0	40.0
30	37.97246161652603	40.0	37.0	40.0	33.0	40.0
31	37.78138962751082	40.0	37.0	40.0	33.0	40.0
32	37.85254054275298	40.0	37.0	40.0	33.0	40.0
33	37.70145658468692	40.0	37.0	40.0	33.0	40.0
34	37.743982710752064	40.0	37.0	40.0	33.0	40.0
35	37.70726957874215	40.0	37.0	40.0	33.0	40.0
36	37.516521640516814	40.0	37.0	40.0	33.0	40.0
37	37.505803927051154	40.0	37.0	40.0	33.0	40.0
38	36.90390307732907	40.0	37.0	40.0	33.0	40.0
39	37.18287111845567	40.0	37.0	40.0	33.0	40.0
40	37.277918599737305	40.0	37.0	40.0	33.0	40.0
41	37.28062556212776	40.0	37.0	40.0	33.0	40.0
42	37.24595848510978	40.0	37.0	40.0	33.0	40.0
43	37.212901611138506	40.0	37.0	40.0	33.0	40.0
44	37.151895538183304	40.0	37.0	40.0	33.0	40.0
45	37.13306810857966	40.0	37.0	40.0	33.0	40.0
46	37.055233161415465	40.0	37.0	40.0	33.0	40.0
47	36.97847453877785	40.0	37.0	40.0	33.0	40.0
48	36.91823475633024	37.0	37.0	40.0	33.0	40.0
49	36.77350920515857	37.0	37.0	40.0	33.0	40.0
50	36.75034465968143	37.0	37.0	40.0	33.0	40.0
51	36.11619197696382	37.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	20.0
9	127.0
10	237.0
11	214.0
12	189.0
13	148.0
14	144.0
15	162.0
16	241.0
17	435.0
18	1057.0
19	2393.0
20	5277.0
21	10478.0
22	19000.0
23	32134.0
24	49486.0
25	73987.0
26	103636.0
27	142057.0
28	191606.0
29	252504.0
30	330105.0
31	433628.0
32	585720.0
33	819616.0
34	1213517.0
35	2001954.0
36	3881438.0
37	8666488.0
38	1.3856932E7
39	356924.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.092393319300776	7.687760587956605	8.87409822522944	48.345747867513175
2	22.030432771929796	12.34311592666974	39.57775734007799	26.048693961322478
3	23.564096104914956	14.136534065491139	22.219109738873613	40.080260090720294
4	27.711434588044227	23.426701476292934	21.291526462376822	27.570337473286017
5	28.55664284592641	25.78356246919073	25.317309570738967	20.342485114143894
6	23.388459608040833	29.992214486020906	26.474101866322446	20.145224039615815
7	18.66369373882348	21.51933380437353	41.16683532707015	18.650137129732844
8	21.016304289746454	21.41867340860228	34.179381184692026	23.385641116959235
9	20.905175212814996	19.51174470682985	36.42777114058279	23.155308939772357
10	22.01316311019613	32.5483497613622	27.068742936932495	18.369744191509174
11	26.48182471352884	25.213064756664167	23.601632593551578	24.703477936255418
12	24.890427753400136	21.873837012927428	28.860482844759055	24.37525238891338
13	23.86544629433651	23.173212873167557	29.419967324192097	23.54137350830384
14	22.75847170064193	24.575855280423937	29.40810994196597	23.257563076968164
15	24.0282599717347	24.01106416777728	28.683863439401776	23.276812421086245
16	24.53456338500105	24.066812309647368	27.841256659030915	23.557367646320667
17	24.35535988205984	25.005425208595035	27.672435956199674	22.966778953145454
18	24.072017641397085	24.720876223376038	27.868211200153453	23.338894935073423
19	24.253261071379605	24.518501971081918	27.51177280091926	23.71646415661922
20	23.94725442369767	24.921185555729362	27.821675173293386	23.309884847279577
21	24.1140863431271	24.439768890997023	27.79151485237328	23.654629913502596
22	24.58911561713799	24.319966369437456	27.26076361611982	23.830154397304735
23	24.676933865575048	24.803308829817905	27.493142302405687	23.02661500220136
24	23.964216300655732	25.13310809583492	27.349578285167624	23.55309731834172
25	24.65389015100394	24.542397771557116	26.95952519044193	23.84418688699702
26	24.182643269930676	25.08911098030131	27.216539894048907	23.511705855719107
27	23.85429995033579	24.87264482884466	27.024747489807936	24.248307731011614
28	24.033767643346007	25.185044086832782	26.76595727675991	24.015230993061305
29	23.90421622954978	25.360034989250835	27.286962565443257	23.44878621575613
30	24.141726432749692	24.503680298223827	26.850041084609472	24.504552184417008
31	24.156038875757098	24.841572334823837	26.565883766410025	24.436505023009044
32	23.949159084165515	24.960835025383552	27.319506965290962	23.77049892515997
33	24.221643710447413	24.47662154236476	26.632333579532673	24.669401167655153
34	24.09265303043165	24.94862525791816	26.688642664177266	24.270079047472926
35	24.459406916812267	24.671582823972198	27.03900141572712	23.83000884348842
36	24.415165158642203	24.5503498487384	26.625495692081646	24.40898930053775
37	24.4296271669118	24.68961253437174	26.551033655114598	24.329726643601862
38	23.920191715519252	25.19584501738155	26.954008250035216	23.929955017063982
39	24.21024805173696	24.545787749941656	26.674650675882333	24.56931352243905
40	24.347256096536466	24.63637118005182	26.395253499283978	24.621119224127735
41	24.761611215761228	24.634371029042182	26.68514231274033	23.91887544245626
42	24.167570141341667	24.423796487571405	26.810749736041693	24.597883635045235
43	24.248983382981866	24.15457150019925	27.075055024907925	24.521390091910963
44	24.70527317278752	24.629195105246726	27.127204678368237	23.538327043597516
45	24.428337502893797	24.31053305234804	27.023277739227453	24.237851705530712
46	24.558763102010094	24.61174225767917	26.387312927195527	24.442181713115207
47	24.882050992043773	24.445045614901662	26.919562716709343	23.75334067634522
48	24.874706567377878	24.372485363297294	26.309824100423775	24.442983968901054
49	25.129951057250516	24.070419409334484	26.461338430876687	24.33829110253831
50	24.648064025172953	24.79956106698288	26.61001747206746	23.94235743577671
51	24.356350316806807	24.862162215838428	26.27895868033211	24.502528787022655
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15489.0
1	15823.5
2	16158.0
3	12112.5
4	8067.0
5	8818.5
6	9570.0
7	10851.5
8	12133.0
9	14526.5
10	16920.0
11	21378.5
12	25837.0
13	31903.0
14	37969.0
15	50405.5
16	62842.0
17	68008.5
18	73175.0
19	74970.5
20	76766.0
21	93151.0
22	109536.0
23	141174.5
24	172813.0
25	212895.0
26	297566.0
27	342155.0
28	424133.0
29	506111.0
30	575351.5
31	644592.0
32	731697.5
33	818803.0
34	936317.5
35	1053832.0
36	1149451.0
37	1245070.0
38	1369077.5
39	1493085.0
40	1625814.5
41	1758544.0
42	1874671.0
43	1990798.0
44	2107446.5
45	2224095.0
46	2298687.0
47	2373279.0
48	2392767.5
49	2412256.0
50	2477892.5
51	2543529.0
52	2458349.0
53	2373169.0
54	2279271.0
55	2185373.0
56	2023950.5
57	1862528.0
58	1688369.0
59	1514210.0
60	1383880.5
61	1253551.0
62	1097810.0
63	942069.0
64	806296.5
65	670524.0
66	570250.5
67	469977.0
68	410930.0
69	351883.0
70	320114.5
71	288346.0
72	247262.0
73	206178.0
74	216605.5
75	160155.0
76	93277.0
77	79377.0
78	65477.0
79	70764.5
80	76052.0
81	73447.5
82	70843.0
83	57880.5
84	44918.0
85	33030.0
86	21142.0
87	16032.0
88	10922.0
89	7979.0
90	5036.0
91	3547.0
92	2058.0
93	1318.0
94	578.0
95	404.5
96	231.0
97	154.5
98	78.0
99	41.5
100	5.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.08660426892715908
2	13.51672638224812
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	2.482451865636748E-4
14	9.687617036631212E-5
15	0.001649922276551253
16	0.0012139795099028487
17	0.0026368482621580576
18	2.421904259157803E-5
19	0.0
20	4.843808518315605E-4
21	0.0
22	0.0
23	0.0
24	1.7861543911288797E-4
25	1.5136901619736266E-5
26	4.54107048592088E-5
27	7.265712777473408E-5
28	3.0273803239472538E-6
29	0.0
30	7.568450809868134E-5
31	1.967797210565715E-4
32	0.0013078282999452137
33	0.0010898569166210112
34	1.9072496040867698E-4
35	0.0023492471313830685
36	3.632856388736704E-5
37	0.0
38	0.0
39	0.0
40	9.08214097184176E-5
41	0.0019011948434388753
42	3.844773011413012E-4
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0025763006556791127
51	0.003036462464919095
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.3031859E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.45568355198792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.28107879676824	25.85491316780921
2	10.514713863259557	7.035538792978239
3	4.0111497675691385	4.02587271910269
4	2.1281319733728963	2.8479243943212476
5	1.2675363839147589	2.120314807544162
6	0.8014879849089827	1.6088597036301233
7	0.5846807742808406	1.3692626474289755
8	0.4304167959702594	1.1519910497153234
9	0.3393825829782831	1.0218848669259948
>10	2.1665564405347317	13.969516540233903
>50	0.2362076367839115	5.539367319830508
>100	0.19829240165847847	13.354710388530911
>500	0.022806864238853263	5.261461094922297
>1k	0.016100268207529154	10.371451767018957
>5k	0.001035567630436167	2.39308866188461
>10k+	4.218979234894128E-4	2.073842078122619
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.296951073810288E-4	0.0	0.0	9.08214097184176E-6	0.0
2	6.296951073810288E-4	0.0	0.0	1.2109521295789013E-5	3.0273803239472534E-6
3	6.32722487704976E-4	0.0	3.0273803239472534E-6	1.5136901619736267E-5	1.816428194368352E-5
4	6.357498680289233E-4	0.0	3.0273803239472534E-6	2.4219042591578027E-5	1.816428194368352E-5
5	6.387772483528705E-4	0.0	3.0273803239472534E-6	2.4219042591578027E-5	2.1191662267630775E-5
6	6.418046286768177E-4	0.0	3.0273803239472534E-6	2.4219042591578027E-5	2.4219042591578027E-5
7	6.539141499726067E-4	3.0273803239472534E-6	3.0273803239472534E-6	2.7246422915525282E-5	2.4219042591578027E-5
8	6.599689106205012E-4	3.0273803239472534E-6	3.0273803239472534E-6	2.7246422915525282E-5	2.7246422915525282E-5
9	6.599689106205012E-4	6.054760647894507E-6	3.0273803239472534E-6	3.0273803239472534E-5	3.0273803239472534E-5
10	6.660236712683958E-4	6.054760647894507E-6	3.0273803239472534E-6	3.0273803239472534E-5	3.3301183563419787E-5
11	6.660236712683958E-4	6.054760647894507E-6	6.054760647894507E-6	3.93559442113143E-5	3.632856388736704E-5
12	6.690510515923431E-4	6.054760647894507E-6	6.054760647894507E-6	4.238332453526155E-5	5.1465465507103306E-5
13	6.720784319162903E-4	6.054760647894507E-6	6.054760647894507E-6	4.238332453526155E-5	5.1465465507103306E-5
14	6.751058122402375E-4	6.054760647894507E-6	6.054760647894507E-6	4.238332453526155E-5	5.4492845831050565E-5
15	6.781331925641848E-4	6.054760647894507E-6	6.054760647894507E-6	4.8438085183156054E-5	5.4492845831050565E-5
16	6.781331925641848E-4	6.054760647894507E-6	6.054760647894507E-6	4.8438085183156054E-5	5.752022615499782E-5
17	6.81160572888132E-4	6.054760647894507E-6	9.08214097184176E-6	5.4492845831050565E-5	5.752022615499782E-5
18	6.962974745078683E-4	6.054760647894507E-6	9.08214097184176E-6	5.4492845831050565E-5	6.054760647894507E-5
19	6.993248548318156E-4	1.2109521295789013E-5	1.2109521295789013E-5	5.752022615499782E-5	6.054760647894507E-5
20	7.114343761276046E-4	1.5136901619736267E-5	1.2109521295789013E-5	5.752022615499782E-5	6.054760647894507E-5
21	7.992284055220749E-4	1.5136901619736267E-5	1.2109521295789013E-5	6.054760647894507E-5	6.054760647894507E-5
22	9.112414775081233E-4	1.5136901619736267E-5	1.2109521295789013E-5	6.357498680289233E-5	6.054760647894507E-5
23	0.001147377142776009	1.5136901619736267E-5	1.2109521295789013E-5	7.265712777473408E-5	6.054760647894507E-5
24	0.0013956223293396838	1.5136901619736267E-5	1.5136901619736267E-5	7.568450809868134E-5	6.054760647894507E-5
25	0.0016590044175230948	1.5136901619736267E-5	1.5136901619736267E-5	8.779402939447034E-5	6.054760647894507E-5
26	0.0020646733809320267	1.5136901619736267E-5	1.5136901619736267E-5	9.08214097184176E-5	6.054760647894507E-5
27	0.0025520816130875346	1.5136901619736267E-5	1.5136901619736267E-5	9.08214097184176E-5	6.054760647894507E-5
28	0.003154530297553038	1.5136901619736267E-5	1.5136901619736267E-5	9.384879004236486E-5	6.357498680289233E-5
29	0.003920457519511694	1.5136901619736267E-5	1.816428194368352E-5	9.990355069025937E-5	6.357498680289233E-5
30	0.004822616856047975	1.816428194368352E-5	1.816428194368352E-5	9.990355069025937E-5	6.357498680289233E-5
31	0.005900364251373197	1.816428194368352E-5	1.816428194368352E-5	9.990355069025937E-5	6.357498680289233E-5
32	0.007229384213586041	1.816428194368352E-5	1.816428194368352E-5	9.990355069025937E-5	6.660236712683957E-5
33	0.008506938710291782	1.816428194368352E-5	1.816428194368352E-5	9.990355069025937E-5	6.962974745078683E-5
34	0.009835958672504627	2.1191662267630775E-5	1.816428194368352E-5	9.990355069025937E-5	6.962974745078683E-5
35	0.011367813116421937	2.1191662267630775E-5	1.816428194368352E-5	1.0293093101420661E-4	7.568450809868134E-5
36	0.013090392520747923	2.1191662267630775E-5	1.816428194368352E-5	1.1201307198604837E-4	8.47666490705231E-5
37	0.01504608021001785	2.1191662267630775E-5	1.816428194368352E-5	1.1201307198604837E-4	8.779402939447034E-5
38	0.01722579404325987	2.1191662267630775E-5	1.816428194368352E-5	1.1504045230999563E-4	9.687617036631211E-5
39	0.019508438807516102	2.1191662267630775E-5	1.816428194368352E-5	1.2109521295789014E-4	9.990355069025937E-5
40	0.022136204928702316	2.4219042591578027E-5	2.1191662267630775E-5	1.301773539297319E-4	1.1201307198604837E-4
>>END_MODULE
