##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8450745_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24238539
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1620123226074	33.0	33.0	33.0	27.0	33.0
2	28.616471314545816	33.0	33.0	33.0	15.0	33.0
3	29.224522113317143	33.0	33.0	33.0	15.0	33.0
4	34.87026932605138	37.0	33.0	37.0	33.0	37.0
5	35.66191122327959	37.0	37.0	37.0	33.0	37.0
6	35.80284748185524	37.0	37.0	37.0	33.0	37.0
7	35.87468106060353	37.0	37.0	37.0	33.0	37.0
8	35.86967865513677	37.0	37.0	37.0	33.0	37.0
9	35.85763935689358	37.0	37.0	37.0	33.0	37.0
10	35.81170077949005	37.0	37.0	37.0	33.0	37.0
11	35.82071559676101	37.0	37.0	37.0	33.0	37.0
12	35.80771943391473	37.0	37.0	37.0	33.0	37.0
13	35.738189541869666	37.0	37.0	37.0	33.0	37.0
14	37.6248726459957	40.0	37.0	40.0	33.0	40.0
15	37.634083885996596	40.0	37.0	40.0	33.0	40.0
16	37.60947522455871	40.0	37.0	40.0	33.0	40.0
17	37.55123924754706	40.0	37.0	40.0	33.0	40.0
18	37.5437979574594	40.0	37.0	40.0	33.0	40.0
19	37.56531183665814	40.0	37.0	40.0	33.0	40.0
20	37.532814828484504	40.0	37.0	40.0	33.0	40.0
21	37.50252117093361	40.0	37.0	40.0	33.0	40.0
22	37.44646370806425	40.0	37.0	40.0	33.0	40.0
23	37.42734267110736	40.0	37.0	40.0	33.0	40.0
24	37.38023236466521	40.0	37.0	40.0	33.0	40.0
25	37.3852639798133	40.0	37.0	40.0	33.0	40.0
26	37.34381663845333	40.0	37.0	40.0	33.0	40.0
27	37.244832000806646	40.0	37.0	40.0	33.0	40.0
28	37.220246855637626	40.0	37.0	40.0	33.0	40.0
29	36.909194939513476	40.0	37.0	40.0	33.0	40.0
30	37.03815989074259	40.0	37.0	40.0	33.0	40.0
31	37.07212464414625	40.0	37.0	40.0	33.0	40.0
32	37.04689960892445	40.0	37.0	40.0	33.0	40.0
33	37.01081513205066	40.0	37.0	40.0	33.0	40.0
34	36.943331155396784	40.0	37.0	40.0	33.0	40.0
35	36.91170016476653	40.0	37.0	40.0	33.0	40.0
36	36.677584238885025	40.0	37.0	40.0	33.0	40.0
37	36.75431732910965	40.0	37.0	40.0	33.0	40.0
38	36.73323301375549	40.0	37.0	40.0	33.0	40.0
39	36.710969708198995	40.0	37.0	40.0	33.0	40.0
40	36.66887311153531	40.0	37.0	40.0	33.0	40.0
41	36.648407851644855	40.0	37.0	40.0	33.0	40.0
42	36.625658006862544	40.0	37.0	40.0	33.0	40.0
43	36.560811111593814	37.0	37.0	40.0	33.0	40.0
44	36.49972752070577	37.0	37.0	40.0	33.0	40.0
45	36.42570606256425	37.0	37.0	40.0	33.0	40.0
46	36.353550228419294	37.0	37.0	40.0	33.0	40.0
47	36.285591305647586	37.0	37.0	40.0	33.0	40.0
48	36.22235597615846	37.0	37.0	40.0	33.0	40.0
49	36.156679492934785	37.0	37.0	40.0	33.0	40.0
50	36.03220148706158	37.0	37.0	40.0	27.0	40.0
51	34.966094161038335	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	39.0
7	494.0
8	1601.0
9	2858.0
10	4747.0
11	8602.0
12	15640.0
13	24664.0
14	34037.0
15	43230.0
16	50768.0
17	56961.0
18	61623.0
19	65922.0
20	70825.0
21	75595.0
22	82020.0
23	90688.0
24	102390.0
25	117166.0
26	135046.0
27	157459.0
28	187087.0
29	224129.0
30	276929.0
31	354358.0
32	471630.0
33	668210.0
34	996616.0
35	1616997.0
36	2899497.0
37	5906612.0
38	9207218.0
39	226880.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	31.50849974909791	14.696702635418744	11.941218899373432	41.853578716109915
2	24.958947188860297	25.572163768567503	29.551306975070773	19.917582067501428
3	22.827419589934856	24.66993575809169	24.866370865009642	27.636273786963812
4	26.713821323966762	29.991848931158767	20.570852063319492	22.723477681554982
5	28.053695810626213	30.601724798677015	20.983405806760878	20.361173583935894
6	22.946769726017852	34.120971448075366	20.83179324517022	22.10046558073656
7	22.135653473173445	19.29559368244101	33.41751332454485	25.151239519840697
8	23.951732231341538	22.234974184702168	25.921178817119106	27.89211476683719
9	25.011023906364127	21.300629517862525	28.198781165226904	25.489565410546444
10	24.469946022068683	33.0163342312121	21.37157324011864	21.142146506600582
11	28.162426894480674	24.8399348269834	19.406052878730694	27.591585399805236
12	27.162408548699513	21.70333767670841	23.25129988732364	27.882953887268435
13	25.637142088348224	24.314607855975463	23.92890446092909	26.119345594747223
14	25.62328841215889	25.46808877033349	24.898411441849973	24.01021137565764
15	25.95659988641781	25.740084095378506	24.683959517211747	23.619356500991938
16	26.545707869507584	25.109905094247686	23.818018436545007	24.526368599699726
17	25.847551382081186	25.396529000734997	24.257924401767205	24.497995215416616
18	25.638197511975523	26.275254262014492	23.84180895385488	24.2447392721551
19	25.545002128930683	24.783088368777005	24.35155081873063	25.320358683561683
20	26.353587677264052	25.129464096740016	24.55850100466235	23.958447221333582
21	26.445772653741713	25.196281377046738	24.44535131697035	23.912594652241204
22	25.675863287065898	25.844805643470153	24.28815786612783	24.19117320333612
23	25.391604555478743	25.19213444039034	24.31120985162133	25.10505115250958
24	25.0694639382443	26.506175553281487	24.6335495680504	23.79081094042381
25	26.36842273548193	25.080433569563905	24.001666781842392	24.549476913111775
26	26.570135627128234	25.573264785733397	23.767524288678032	24.08907529846034
27	25.023108171741853	27.06461504032434	24.056036909040714	23.85623987889309
28	25.727368374452865	25.68266951148483	25.00979773166267	23.58016438239964
29	26.374477644983507	25.475283963173922	23.73910514911692	24.411133242725647
30	26.20240654380563	26.091116630797416	24.404295728757084	23.30218109663987
31	25.285065295440347	25.748608810392348	24.282168524962525	24.684157369204783
32	25.30219734539093	25.599805549986606	24.917596608066912	24.180400496555553
33	25.338518773013263	26.35923356176934	24.15771297568961	24.144534689527788
34	25.846665227521864	26.302805639203097	23.598294415358975	24.252234717916068
35	26.012386862675434	26.06831007071187	23.799372582370275	24.119930484242417
36	24.82802394395231	25.775270371281785	24.443772164404486	24.95293352036142
37	25.493597240396483	25.045353525205353	24.4386937805153	25.022355453882867
38	25.358934628829328	25.979104482784827	23.971977736468332	24.68998315191752
39	24.965566886385083	26.07038052509962	24.575382691918605	24.38866989659669
40	25.360365880074013	24.78760760723591	24.677022541562746	25.175003971127325
41	25.627821293818887	25.922537550206187	23.87660881566	24.573032340314924
42	25.611342162278333	25.19333765633705	24.107821439235124	25.087498742149496
43	25.25622982642911	24.827913530251756	24.570883320827004	25.34497332249213
44	25.942611572850232	24.341494514821445	24.7392426249857	24.976651287342623
45	24.72549368846416	25.01639704223227	25.015509990818813	25.242599278484757
46	25.548908009700234	24.123764525567545	24.997898903877648	25.329428560854574
47	24.846266299897906	24.863788125441914	25.34605708591784	24.943888488742342
48	24.963283118973255	25.64796213007049	24.916970565721556	24.4717841852347
49	25.310348569293744	25.462571333146713	23.866590228655962	25.360489868903585
50	25.7467191541489	25.44162638028229	24.529001039324765	24.282653426244043
51	25.50376071499607	24.696448928047598	24.50858130029763	25.291209056658698
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4190.0
1	2918.5
2	1647.0
3	1454.5
4	1262.0
5	1490.0
6	1718.0
7	2166.5
8	2615.0
9	3329.5
10	4044.0
11	5504.5
12	6965.0
13	9436.0
14	11907.0
15	17862.5
16	23818.0
17	28355.5
18	32893.0
19	42300.0
20	51707.0
21	67716.0
22	83725.0
23	100613.0
24	117501.0
25	141296.0
26	199907.5
27	234724.0
28	275553.5
29	316383.0
30	358086.5
31	399790.0
32	448952.5
33	498115.0
34	563294.5
35	628474.0
36	710887.0
37	793300.0
38	877701.5
39	962103.0
40	1085760.0
41	1209417.0
42	1354101.0
43	1498785.0
44	1487519.0
45	1476253.0
46	1587123.0
47	1697993.0
48	1731473.5
49	1764954.0
50	1772831.5
51	1780709.0
52	1758346.0
53	1735983.0
54	1624464.0
55	1512945.0
56	1526115.0
57	1539285.0
58	1322772.5
59	1106260.0
60	1084219.0
61	1062178.0
62	893018.0
63	723858.0
64	624990.5
65	526123.0
66	451528.0
67	376933.0
68	374838.0
69	372743.0
70	349688.5
71	326634.0
72	271323.5
73	216013.0
74	204867.5
75	165623.5
76	137525.0
77	135893.0
78	134261.0
79	144174.5
80	154088.0
81	139529.5
82	124971.0
83	102424.0
84	79877.0
85	66751.5
86	53626.0
87	55000.5
88	56375.0
89	39325.0
90	22275.0
91	15208.5
92	8142.0
93	5707.5
94	3273.0
95	2112.5
96	952.0
97	646.5
98	341.0
99	207.0
100	73.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	4.490699707602013
3	0.0
4	0.0
5	0.0
6	8.58137530483995E-4
7	0.0
8	1.6502644816999904E-5
9	0.0012665779897047425
10	0.0012376983612749925
11	0.001184064765619743
12	3.7956083079099777E-4
13	9.282737709562446E-4
14	3.34178557544248E-4
15	0.0024093861432819856
16	0.00333765991423823
17	0.0011304311699644933
18	0.0014563584051002414
19	0.004204048767130725
20	0.0022484853563162367
21	0.005755297379928716
22	0.0028302035861154833
23	0.00339541917109773
24	2.5991665586774847E-4
25	8.828914977094947E-4
26	0.0016255105144744903
27	0.0059492034565284645
28	0.0032427697065404806
29	0.0029415964386302327
30	1.8152909298699892E-4
31	0.006386523544178962
32	0.008152306539597953
33	0.007013624047224958
34	0.007933646495772703
35	0.007145645205760959
36	0.010755598759479686
37	0.007112639916126959
38	0.008057416331900201
39	0.0010025356726327441
40	0.004492845051428223
41	0.004080278931003226
42	0.003143753837638481
43	5.899695522077465E-4
44	0.0024176374656904856
45	0.003861618887177977
46	0.004909536833057471
47	8.127552572372451E-4
48	0.004331944264462474
49	0.0042659336851944745
50	0.00680321532580821
51	8.086295960329953E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.4238539E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.87476595590909
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.70285014275596	31.323510930435045
2	8.990018220108915	6.809896720519671
3	2.803378742888475	3.1853194131801486
4	1.4131114045480864	2.1408505486753895
5	0.8292283200849886	1.5703414273615306
6	0.5563025937815843	1.2641898324085603
7	0.3731340920968383	0.9892656485836864
8	0.2869521201494148	0.8694595512968792
9	0.22310368190399302	0.7604999762413791
>10	1.463214018813678	10.882385763157652
>50	0.17999466296858474	4.772069081262412
>100	0.14326554217408927	10.87464556076247
>500	0.01800001460314614	4.73121254738251
>1k	0.015290861768115485	11.385130802754661
>5k	0.0016688371774322517	4.326721737571352
>10k+	4.867441767510726E-4	4.114500458406549
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC	158797	0.6551426222512834	No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT	119044	0.4911352123987341	No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCG	42072	0.17357481818520498	No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG	40519	0.16716766633500477	No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA	34541	0.14250446365599842	No Hit
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCG	32659	0.13473996926959994	No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT	25506	0.10522911467559988	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.66388852892495E-5	0.0	0.0	0.0	0.0
2	9.076454649349946E-5	0.0	0.0	0.0	0.0
3	9.076454649349946E-5	0.0	0.0	8.251322408499952E-6	0.0
4	9.489020769774944E-5	0.0	0.0	8.251322408499952E-6	0.0
5	9.489020769774944E-5	0.0	0.0	8.251322408499952E-6	0.0
6	9.489020769774944E-5	0.0	0.0	8.251322408499952E-6	0.0
7	9.489020769774944E-5	4.125661204249976E-6	0.0	1.2376983612749926E-5	0.0
8	9.901586890199941E-5	8.251322408499952E-6	0.0	1.2376983612749926E-5	0.0
9	1.0314153010624939E-4	8.251322408499952E-6	0.0	1.2376983612749926E-5	0.0
10	1.0726719131049937E-4	8.251322408499952E-6	0.0	1.2376983612749926E-5	0.0
11	1.1139285251474934E-4	8.251322408499952E-6	0.0	1.2376983612749926E-5	0.0
12	1.1139285251474934E-4	8.251322408499952E-6	0.0	1.2376983612749926E-5	0.0
13	1.1551851371899932E-4	8.251322408499952E-6	0.0	1.2376983612749926E-5	0.0
14	1.2376983612749927E-4	8.251322408499952E-6	0.0	1.2376983612749926E-5	0.0
15	1.2789549733174924E-4	8.251322408499952E-6	0.0	1.2376983612749926E-5	0.0
16	1.3202115853599923E-4	8.251322408499952E-6	0.0	1.2376983612749926E-5	0.0
17	1.361468197402492E-4	8.251322408499952E-6	0.0	1.2376983612749926E-5	0.0
18	1.4852380335299913E-4	8.251322408499952E-6	0.0	2.4753967225499852E-5	0.0
19	1.7740343178274895E-4	8.251322408499952E-6	0.0	2.887962842974983E-5	0.0
20	2.0628306021249878E-4	8.251322408499952E-6	0.0	4.538227324674973E-5	0.0
21	2.3103702743799864E-4	1.2376983612749926E-5	0.0	4.538227324674973E-5	0.0
22	3.011732679102482E-4	1.2376983612749926E-5	0.0	4.9507934450999705E-5	0.0
23	3.9606347560799764E-4	1.2376983612749926E-5	0.0	5.3633595655249686E-5	0.0
24	5.11581989326997E-4	1.2376983612749926E-5	0.0	5.3633595655249686E-5	0.0
25	6.972367435182458E-4	1.2376983612749926E-5	0.0	5.3633595655249686E-5	4.125661204249976E-6
26	9.282737709562446E-4	1.2376983612749926E-5	0.0	5.775925685949966E-5	4.125661204249976E-6
27	0.0012954576181344925	1.2376983612749926E-5	0.0	6.601057926799962E-5	4.125661204249976E-6
28	0.0016213848532702405	1.2376983612749926E-5	0.0	7.013624047224958E-5	4.125661204249976E-6
29	0.0021040872141674877	1.2376983612749926E-5	0.0	7.838756288074953E-5	4.125661204249976E-6
30	0.0026569258155369844	1.2376983612749926E-5	0.0	7.838756288074953E-5	4.125661204249976E-6
31	0.0033376599142382305	1.2376983612749926E-5	0.0	8.251322408499951E-5	4.125661204249976E-6
32	0.0041504151714754755	1.6502644816999904E-5	0.0	8.251322408499951E-5	4.125661204249976E-6
33	0.00504155799159347	1.6502644816999904E-5	0.0	8.251322408499951E-5	4.125661204249976E-6
34	0.006089475937472964	1.6502644816999904E-5	0.0	8.66388852892495E-5	4.125661204249976E-6
35	0.007166273511782208	2.0628306021249878E-5	0.0	8.66388852892495E-5	4.125661204249976E-6
36	0.008329709971380701	2.0628306021249878E-5	0.0	9.076454649349946E-5	4.125661204249976E-6
37	0.009505523414591944	2.0628306021249878E-5	0.0	9.489020769774944E-5	8.251322408499952E-6
38	0.011036143721368685	2.0628306021249878E-5	0.0	9.489020769774944E-5	1.2376983612749926E-5
39	0.012562638366941176	2.0628306021249878E-5	0.0	9.901586890199941E-5	1.2376983612749926E-5
40	0.014464568182100415	2.4753967225499852E-5	0.0	1.0314153010624939E-4	1.6502644816999904E-5
>>END_MODULE
