[2019-06-26 12:52:26] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2019-06-26 12:52:26] Checking for Bowtie Bowtie version: 2.3.4.1 [2019-06-26 12:52:26] Checking for Bowtie index files (genome).. [2019-06-26 12:52:26] Checking for reference FASTA file [2019-06-26 12:52:26] Generating SAM header for Bowtie2Index/genome [2019-06-26 12:52:31] Reading known junctions from GTF file [2019-06-26 12:52:36] Preparing reads left reads: min. length=51, max. length=51, 24194001 kept reads (44538 discarded) right reads: min. length=51, max. length=51, 24226468 kept reads (12071 discarded) [2019-06-26 13:08:27] Building transcriptome data files /scratch/7076164.1.linga/tophat2/tmp/RefSeq_GeneBody [2019-06-26 13:08:47] Building Bowtie index from RefSeq_GeneBody.fa [2019-06-26 13:16:04] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-06-26 13:26:39] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-06-26 13:36:42] Resuming TopHat pipeline with unmapped reads [2019-06-26 13:36:42] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2019-06-26 13:46:48] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2019-06-26 13:47:51] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2019-06-26 13:49:15] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2019-06-26 13:58:56] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2019-06-26 13:59:54] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2019-06-26 14:01:18] Searching for junctions via segment mapping [2019-06-26 14:06:06] Retrieving sequences for splices [2019-06-26 14:08:31] Indexing splices [2019-06-26 14:08:48] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2019-06-26 14:08:59] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2019-06-26 14:09:10] Joining segment hits [2019-06-26 14:12:19] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2019-06-26 14:12:29] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2019-06-26 14:12:43] Joining segment hits [2019-06-26 14:15:37] Reporting output tracks ----------------------------------------------- [2019-06-26 15:10:23] A summary of the alignment counts can be found in /scratch/7076164.1.linga/tophat2/align_summary.txt [2019-06-26 15:10:23] Run complete: 02:17:57 elapsed