[2019-07-17 22:17:11] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2019-07-17 22:17:11] Checking for Bowtie Bowtie version: 2.3.4.1 [2019-07-17 22:17:13] Checking for Bowtie index files (genome).. [2019-07-17 22:17:13] Checking for reference FASTA file [2019-07-17 22:17:13] Generating SAM header for Bowtie2Index/genome [2019-07-17 22:17:18] Reading known junctions from GTF file [2019-07-17 22:17:22] Preparing reads left reads: min. length=51, max. length=51, 18516200 kept reads (43568 discarded) right reads: min. length=51, max. length=51, 18546207 kept reads (13561 discarded) [2019-07-17 22:28:13] Building transcriptome data files /scratch/7776691.1.linga/tophat2/tmp/RefSeq_GeneBody [2019-07-17 22:28:30] Building Bowtie index from RefSeq_GeneBody.fa [2019-07-17 22:35:01] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-07-17 22:43:18] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2019-07-17 22:51:17] Resuming TopHat pipeline with unmapped reads [2019-07-17 22:51:18] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2019-07-17 22:59:21] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2019-07-17 23:00:17] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2019-07-17 23:01:29] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2019-07-17 23:08:42] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/2) [2019-07-17 23:09:29] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/2) [2019-07-17 23:10:34] Searching for junctions via segment mapping [2019-07-17 23:15:17] Retrieving sequences for splices [2019-07-17 23:17:45] Indexing splices [2019-07-17 23:18:06] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2019-07-17 23:18:14] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2019-07-17 23:18:24] Joining segment hits [2019-07-17 23:21:26] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/2) [2019-07-17 23:21:35] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/2) [2019-07-17 23:21:46] Joining segment hits [2019-07-17 23:24:39] Reporting output tracks ----------------------------------------------- [2019-07-18 01:25:19] A summary of the alignment counts can be found in /scratch/7776691.1.linga/tophat2/align_summary.txt [2019-07-18 01:25:19] Run complete: 03:08:08 elapsed