FastQCFastQC Report
Fri 5 Jul 2019
SRR8315054_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8315054_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71616439
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3777610.5274780557017084No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1183480.16525256163602325No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG925680.1292552398479349Illumina Single End PCR Primer 1 (100% over 50bp)
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG785310.10965499136308635No Hit
CGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGATCAAAAC738860.10316905033493776No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)