##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8315054_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	71616439
Sequences flagged as poor quality	0
Sequence length	151
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.626660870418313	32.0	32.0	32.0	12.0	32.0
2	30.620532640557567	32.0	32.0	32.0	27.0	32.0
3	33.81225332915533	37.0	32.0	37.0	32.0	37.0
4	34.59110146484664	37.0	37.0	37.0	32.0	37.0
5	35.199652764081165	37.0	37.0	37.0	32.0	37.0
6	38.08150972153195	41.0	37.0	41.0	32.0	41.0
7	37.05985519609541	41.0	37.0	41.0	27.0	41.0
8	37.689052676858175	41.0	37.0	41.0	32.0	41.0
9	38.25076681067597	41.0	37.0	41.0	32.0	41.0
10-14	38.51434529158871	41.0	41.0	41.0	32.0	41.0
15-19	38.5433046119481	41.0	41.0	41.0	32.0	41.0
20-24	38.563103976728016	41.0	41.0	41.0	32.0	41.0
25-29	38.53273920531011	41.0	41.0	41.0	32.0	41.0
30-34	38.475857293044136	41.0	41.0	41.0	32.0	41.0
35-39	38.37183520951105	41.0	41.0	41.0	32.0	41.0
40-44	38.29791110948703	41.0	41.0	41.0	32.0	41.0
45-49	38.10072708865069	41.0	38.6	41.0	32.0	41.0
50-54	38.006334562376104	41.0	37.0	41.0	31.0	41.0
55-59	37.96194816388455	41.0	37.0	41.0	27.0	41.0
60-64	37.90314179123036	41.0	37.0	41.0	27.0	41.0
65-69	37.790074991022664	41.0	37.0	41.0	27.0	41.0
70-74	37.699157161388605	41.0	37.0	41.0	27.0	41.0
75-79	37.11532242478573	41.0	37.0	41.0	26.0	41.0
80-84	37.4152458850963	41.0	37.0	41.0	27.0	41.0
85-89	37.268939291996915	41.0	37.0	41.0	27.0	41.0
90-94	37.16904497583299	41.0	37.0	41.0	27.0	41.0
95-99	36.969019018105605	41.0	37.0	41.0	24.0	41.0
100-104	36.71005219904888	41.0	37.0	41.0	22.0	41.0
105-109	36.46442498767637	41.0	37.0	41.0	22.0	41.0
110-114	36.10519076493038	41.0	37.0	41.0	22.0	41.0
115-119	35.803991483016915	41.0	36.0	41.0	22.0	41.0
120-124	35.34063594253828	41.0	32.0	41.0	22.0	41.0
125-129	34.869862680550206	41.0	32.0	41.0	12.0	41.0
130-134	34.371535068924615	41.0	32.0	41.0	12.0	41.0
135-139	33.8231450603122	41.0	29.0	41.0	12.0	41.0
140-144	33.162079418665314	37.0	27.0	41.0	12.0	41.0
145-149	32.58169408562746	37.0	27.0	41.0	12.0	41.0
150-151	30.047539099228324	34.5	24.5	39.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	48.0
5	208.0
6	373.0
7	190.0
8	153.0
9	128.0
10	163.0
11	191.0
12	278.0
13	939.0
14	3759.0
15	14291.0
16	44797.0
17	106429.0
18	189192.0
19	277295.0
20	364725.0
21	452857.0
22	540020.0
23	635721.0
24	767950.0
25	858648.0
26	947785.0
27	1065966.0
28	1197732.0
29	1347438.0
30	1521740.0
31	1709151.0
32	1931911.0
33	2202086.0
34	2553601.0
35	3019172.0
36	3667680.0
37	4675393.0
38	6486186.0
39	1.1110448E7
40	2.3921794E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.74265866691882	11.636163421140763	13.382550733050033	42.23862717889039
2	27.8267002748046	20.981506755475174	28.480549898108677	22.711243071611545
3	30.00494014318399	24.68941087696242	18.887764762151672	26.41788421770192
4	31.5134031013356	31.361900384557927	16.030241100400826	21.094455413705646
5	29.190752655777196	31.028839179179318	19.268040063044843	20.512368101998646
6	21.130874771062256	32.18563400505631	22.56614744432257	24.11734377955887
7	19.135709446269356	14.537045053277382	38.69303827693737	27.63420722351589
8	21.497046630343817	17.509728446886186	26.361138117799022	34.63208680497098
9	23.456982181713283	18.15905021156146	28.791826624788687	29.59214098193657
10-14	25.434704756581176	24.565105192436647	24.27762019651484	25.722569854467338
15-19	26.07220131874815	22.977556068077472	24.92236300221564	26.02787961095874
20-24	26.276792706180725	23.929979789831936	24.388464535767774	25.40476296821957
25-29	26.4192312094117	23.686838622989313	24.198582425701886	25.695347741897102
30-34	26.163315079136872	23.763886010981743	24.402947755676575	25.669851154204814
35-39	26.109817101864902	23.566369902845754	24.248135946219186	26.075677049070155
40-44	26.609743377291394	23.625678986911165	24.10604494346444	25.658532692333004
45-49	26.642077589348617	23.35920304424549	23.792463829194652	26.20625553721124
50-54	26.529170378965965	23.458321060167382	23.926444601369422	26.08606395949723
55-59	26.74566746209098	23.234850373522818	23.681273829386846	26.338208334999358
60-64	26.716699183499788	23.499554155322166	23.776959312095	26.006787349083048
65-69	26.69014983534794	23.195823278896544	23.839551143856294	26.274475741899227
70-74	26.488528809369495	23.234453623613387	24.159856860129672	26.11716070688745
75-79	26.891836792073775	23.031630552801268	24.127798210234793	25.948734444890164
80-84	26.55019959772295	23.00745752330517	24.043231351797232	26.399111527174647
85-89	26.942439290081783	23.386979682507825	23.758935105358095	25.9116459220523
90-94	26.779125781389197	23.137338152415197	23.968140016596227	26.115396049599383
95-99	26.991088696135623	23.379491039590153	23.45803547641879	26.171384787855434
100-104	26.936264076446015	23.691716296284536	23.37343683234236	25.99858279492709
105-109	27.572383551101844	23.5339033636147	23.399101754774275	25.49461133050918
110-114	27.644515987812373	24.065807876453846	23.000725685928966	25.288950449804815
115-119	28.53220113660866	24.113776543642924	22.884198586557858	24.46982373319056
120-124	28.827258016892397	24.424731053556965	22.700954575461736	24.047056354088898
125-129	29.59802919621321	24.489052051980135	22.37667722570256	23.536241526104092
130-134	29.553616116371238	24.566195789374593	22.658290541109945	23.221897553144224
135-139	30.118772549844756	25.02007982503785	22.522170426658583	22.338977198458814
140-144	30.709988730465295	25.000122612734256	22.455106227148296	21.834782429652158
145-149	30.759107489566407	25.072105972279328	22.71770691549714	21.451079622657126
150-151	30.4423776546258	25.425240012291066	22.666737158580137	21.465645174502995
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	356697.0
1	227313.0
2	82523.5
3	58921.0
4	45350.5
5	37525.0
6	58739.5
7	68655.5
8	44676.0
9	30403.5
10	24415.5
11	21744.0
12	19756.0
13	17570.5
14	16517.0
15	16588.5
16	16782.0
17	17645.0
18	19617.0
19	22509.5
20	28750.0
21	39501.5
22	52628.0
23	69647.0
24	94115.0
25	133434.5
26	197970.5
27	334850.5
28	392476.5
29	377131.5
30	483406.5
31	567006.0
32	615101.0
33	690581.0
34	742067.0
35	827686.0
36	887602.0
37	884931.0
38	852687.5
39	844775.0
40	970817.5
41	1088427.5
42	1137663.0
43	1238901.5
44	1459386.5
45	1639163.5
46	1857317.5
47	2131770.0
48	2316488.0
49	2463588.0
50	2592305.5
51	2560489.5
52	2514776.5
53	2521041.0
54	2425100.0
55	2489799.5
56	2446491.0
57	2226271.0
58	2306149.5
59	2470447.0
60	2234077.5
61	1853136.0
62	1568020.5
63	1255420.0
64	1077873.5
65	1045728.5
66	970646.0
67	911147.5
68	825467.0
69	761255.0
70	770965.5
71	732298.5
72	792503.0
73	758623.0
74	572809.5
75	408548.5
76	394553.5
77	419527.5
78	325131.5
79	313043.0
80	308308.0
81	322625.0
82	405218.5
83	428678.0
84	358559.0
85	299643.5
86	283878.5
87	168744.0
88	34103.0
89	7595.0
90	3072.5
91	1344.5
92	768.5
93	529.5
94	407.5
95	366.5
96	357.5
97	341.5
98	366.5
99	545.0
100	1108.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.325048889962262
2	0.04993266978828701
3	0.015676568336496038
4	0.02566868760397316
5	1.6057765731691852E-4
6	0.02336614363079404
7	0.0035732019571651694
8	0.00204143073910726
9	0.0015080336513241044
10-14	0.005690872175311593
15-19	0.005131503396866744
20-24	0.0051312241313757586
25-29	0.005972371790225426
30-34	0.005345420762961978
35-39	0.00527644218668845
40-44	0.005346258559434936
45-49	0.004816771188525584
50-54	0.008772566868341498
55-59	0.005249074168571828
60-64	0.006289338122494474
65-69	0.007586247062633204
70-74	0.008426277659518927
75-79	0.008871147586659538
80-84	0.006003370259724866
85-89	0.01014655308399235
90-94	0.007615290673695742
95-99	0.008406449809658925
100-104	0.009045409253034768
105-109	0.011510206476476721
110-114	0.010086511003430372
115-119	0.0148706081295106
120-124	0.008418737491262306
125-129	0.015324414552362763
130-134	0.014139491074109396
135-139	0.014990133759652585
140-144	0.01238346966678977
145-149	0.013709701483482025
150-151	0.02072917923215925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	7.1616439E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.485040792059031
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.09792323958088	8.25235267916554
2	8.403011223096696	2.098238757929842
3	5.7166158205817466	2.1411654513747935
4	4.52152740412353	2.2580581633158023
5	3.369787410909687	2.1035966642887214
6	2.3548932431291663	1.7640562920847096
7	1.678362627317577	1.4668098106149123
8	1.2016153308511648	1.2001773137632241
9	0.8963261823447927	1.007160205460875
>10	4.695593867356738	11.089033073408935
>50	0.4892367334383121	4.245043143057272
>100	0.4162667062773974	10.759552750615988
>500	0.06673093075684045	5.834851431672228
>1k	0.07394648664191818	19.830337252566103
>5k	0.011578200621076788	10.014071191626996
>10k+	0.006584592973688106	15.9354958190543
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	377761	0.5274780557017084	No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT	118348	0.16525256163602325	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	92568	0.1292552398479349	Illumina Single End PCR Primer 1 (100% over 50bp)
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG	78531	0.10965499136308635	No Hit
CGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGATCAAAAC	73886	0.10316905033493776	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.016067302648209E-4	6.981637274648632E-6	0.0	0.0	0.0
2	3.337222617282046E-4	6.981637274648632E-6	0.0	1.3963274549297264E-6	4.188982364789179E-6
3	3.742157579211667E-4	6.981637274648632E-6	0.0	1.3963274549297264E-6	9.774292184508084E-6
4	4.412394757577935E-4	6.981637274648632E-6	0.0	1.3963274549297264E-6	9.774292184508084E-6
5	5.236227955986474E-4	6.981637274648632E-6	0.0	5.5853098197189055E-6	9.774292184508084E-6
6	6.004208056197823E-4	6.981637274648632E-6	0.0	5.5853098197189055E-6	1.2566947094367538E-5
7	7.051453647395118E-4	6.981637274648632E-6	0.0	1.535960200422699E-5	1.2566947094367538E-5
8	9.104055006141816E-4	1.2566947094367538E-5	0.0	1.535960200422699E-5	1.2566947094367538E-5
9	9.84410855725457E-4	1.2566947094367538E-5	0.0	1.535960200422699E-5	1.2566947094367538E-5
10-14	0.001443802588397337	1.368400905831132E-5	0.0	3.127773499042587E-5	1.2566947094367538E-5
15-19	0.0032417138193648527	1.3963274549297263E-5	0.0	4.524100953972314E-5	1.2846212585353482E-5
20-24	0.005894456718240347	1.48010710222551E-5	0.0	7.45638860932474E-5	1.9827849860002114E-5
25-29	0.009069146819768573	1.899005338704428E-5	0.0	1.0472455911972947E-4	2.373756673380535E-5
30-34	0.019419284446689677	2.2341239278875622E-5	0.0	1.0891354148451866E-4	2.373756673380535E-5
35-39	0.045508545880087674	3.6304513828172885E-5	0.0	1.203634266149424E-4	2.4575363206763183E-5
40-44	0.05631723744320769	5.7249425652118783E-5	0.0	1.3265110821832402E-4	2.5133894188735075E-5
45-49	0.06369599024603834	5.7249425652118783E-5	0.0	1.4801071022255103E-4	2.7926549098594525E-5
50-54	0.07438739030294426	5.92042840890204E-5	1.117061963943781E-6	1.5331675455128394E-4	2.9322876553524255E-5
55-59	0.08946353783382052	6.702371783662686E-5	1.3963274549297264E-6	1.6141545378987635E-4	2.9322876553524255E-5
60-64	0.11409698826270878	6.897857627352849E-5	2.7926549098594528E-6	1.7593725932114554E-4	2.9322876553524255E-5
65-69	0.15662716768143134	7.177123118338793E-5	1.2846212585353482E-5	2.083320562755152E-4	2.9322876553524255E-5
70-74	0.22986202930307664	7.791507198507873E-5	1.6197398477184826E-5	2.3318668497326428E-4	3.071920400845398E-5
75-79	0.3546138338433722	9.634659439015113E-5	1.6755929459156716E-5	2.443573046127021E-4	3.071920400845398E-5
80-84	0.5787821983162275	9.774292184508083E-5	1.6755929459156716E-5	2.6362662349073234E-4	3.127773499042587E-5
85-89	0.9593610204495089	9.774292184508083E-5	1.7872991423100494E-5	3.454514123496143E-4	3.51874518642291E-5
90-94	1.5830803874512667	1.0221116970085596E-4	1.8152256914086442E-5	3.9236801483525307E-4	3.7142310301130715E-5
95-99	2.566876021300082	1.0528309010170137E-4	2.066564633295995E-5	4.412394757577935E-4	3.853863775606045E-5
100-104	4.0365693133667255	1.1198546188536407E-4	2.708875262563669E-5	4.973718394459686E-4	3.909716873803234E-5
105-109	6.068353105353367	1.3376817018226778E-4	3.546671735521504E-5	5.35910477202029E-4	4.579954052169502E-5
110-114	8.618165725888716	1.4102907294790237E-4	3.6304513828172885E-5	5.984659471828808E-4	4.74751334676107E-5
115-119	11.75548675353713	1.4102907294790237E-4	3.6304513828172885E-5	6.657689305104936E-4	4.74751334676107E-5
120-124	15.212598325364933	1.4186686942086022E-4	3.7142310301130715E-5	7.442425334775443E-4	4.7754398958596645E-5
125-129	18.980126336636204	1.4493878982170562E-4	4.3286151102821524E-5	7.869701535983938E-4	5.166411583239988E-5
130-134	22.849142219986668	1.5080336513241044E-4	4.524100953972313E-5	8.338867560840327E-4	5.5015301724231226E-5
135-139	26.825501614231335	1.5192042709635424E-4	4.74751334676107E-5	8.514804820161472E-4	5.6411629179160946E-5
140	29.065318369152646	1.5499234749719962E-4	4.8871460922540424E-5	8.657230220564304E-4	5.864575310704851E-5
>>END_MODULE
