FastQCFastQC Report
Fri 5 Jul 2019
SRR8315054_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8315054_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71616439
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1821120.25428798547216236No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT1363160.1903417733462006No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTCGGTTATCTCGTAT914370.1276759934964094TruSeq Adapter, Index 27 (97% over 37bp)
CGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGATCAAAAC827980.11561312061327148No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC820570.11457844196916857No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC794720.11096893549817521No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA794210.1108977227979738No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG740950.10346088277301808No Hit
GTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGTCGCGCCCGCCCGC736580.10285068767521378No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)