##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8315052_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	71519666
Sequences flagged as poor quality	0
Sequence length	151
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.6361553478172	32.0	32.0	32.0	12.0	32.0
2	30.9225592160903	32.0	32.0	32.0	32.0	32.0
3	33.278760026647774	37.0	32.0	37.0	27.0	37.0
4	34.693245938816325	37.0	37.0	37.0	32.0	37.0
5	35.15066001566618	37.0	37.0	37.0	32.0	37.0
6	37.796440590200746	41.0	37.0	41.0	32.0	41.0
7	38.17798989721233	41.0	37.0	41.0	32.0	41.0
8	37.9809784766053	41.0	37.0	41.0	32.0	41.0
9	38.37168540468296	41.0	37.0	41.0	32.0	41.0
10-14	38.54109985077391	41.0	41.0	41.0	32.0	41.0
15-19	38.57467493206694	41.0	41.0	41.0	32.0	41.0
20-24	38.582363323676596	41.0	41.0	41.0	32.0	41.0
25-29	38.55056496768315	41.0	41.0	41.0	32.0	41.0
30-34	38.49488815006491	41.0	41.0	41.0	32.0	41.0
35-39	38.39565771462076	41.0	41.0	41.0	32.0	41.0
40-44	38.33489654160297	41.0	41.0	41.0	32.0	41.0
45-49	38.15164962319595	41.0	41.0	41.0	32.0	41.0
50-54	38.0622858557533	41.0	39.4	41.0	31.0	41.0
55-59	38.0188195118249	41.0	37.0	41.0	31.0	41.0
60-64	37.96686299401902	41.0	37.0	41.0	29.0	41.0
65-69	37.8738879541188	41.0	37.0	41.0	27.0	41.0
70-74	37.79470456978923	41.0	37.0	41.0	27.0	41.0
75-79	37.21756878450747	41.0	37.0	41.0	26.0	41.0
80-84	37.521993752040174	41.0	37.0	41.0	27.0	41.0
85-89	37.38792970313928	41.0	37.0	41.0	27.0	41.0
90-94	37.29764009803961	41.0	37.0	41.0	27.0	41.0
95-99	37.11245906545481	41.0	37.0	41.0	27.0	41.0
100-104	36.864584174652045	41.0	37.0	41.0	22.0	41.0
105-109	36.63068072773159	41.0	37.0	41.0	22.0	41.0
110-114	36.28454065207743	41.0	37.0	41.0	22.0	41.0
115-119	35.999800742917344	41.0	37.0	41.0	22.0	41.0
120-124	35.54424019541702	41.0	33.0	41.0	22.0	41.0
125-129	35.079180134873674	41.0	32.0	41.0	18.0	41.0
130-134	34.5970638789057	41.0	32.0	41.0	12.0	41.0
135-139	34.0485479616194	41.0	31.0	41.0	12.0	41.0
140-144	33.387765286823345	37.8	27.0	41.0	12.0	41.0
145-149	32.82764746971833	37.0	27.0	41.0	12.0	41.0
150-151	30.26120033893894	34.5	24.5	39.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	35.0
5	205.0
6	422.0
7	171.0
8	142.0
9	144.0
10	136.0
11	199.0
12	282.0
13	840.0
14	3502.0
15	12690.0
16	41229.0
17	99032.0
18	177114.0
19	260259.0
20	340632.0
21	422251.0
22	506362.0
23	594858.0
24	710940.0
25	806948.0
26	906337.0
27	1023269.0
28	1158102.0
29	1308798.0
30	1478564.0
31	1670324.0
32	1892231.0
33	2173375.0
34	2525751.0
35	2991884.0
36	3647872.0
37	4661678.0
38	6498823.0
39	1.1158604E7
40	2.4445661E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.75908040796089	9.952932707843958	12.473337647029629	43.814649237165526
2	28.260981050975527	19.591039816268164	27.699604946779644	24.44837418597667
3	31.558554252505715	23.22255794505129	17.14883594153766	28.070051860905338
4	33.97338017208256	30.294462114071187	14.347383228519053	21.384774485327203
5	30.547718577171835	30.002411787869438	18.196436815177698	21.25343281978103
6	22.015568997958738	31.823307395539054	21.759159684664976	24.401963921837236
7	19.40074701287903	13.712969401852343	38.17808653809221	28.70819704717642
8	21.948732750040406	16.204787337311892	25.345717889951136	36.50076202269657
9	24.65919280567472	16.678437032944863	27.57960692457723	31.082763236803185
10-14	26.204632870219623	23.89909606259947	23.114789411927752	26.781481655253152
15-19	26.909715591525785	22.21283567687029	23.776818582095636	27.100630149508287
20-24	27.20554105769113	23.095037762586053	23.308407767743304	26.391013411979515
25-29	27.380304998789285	22.81136800321754	23.147733979951227	26.66059301804195
30-34	27.08839151142659	22.95507054083641	23.34563208143593	26.61090586630107
35-39	26.97350486343155	22.880674730939248	23.088128489941777	27.057691915687425
40-44	27.6753203401909	22.94458173691302	22.875322492438343	26.504775430457737
45-49	27.70501111161704	22.662528754484242	22.54465748292464	27.08780265097408
50-54	27.449771106193904	22.75874587635776	22.66810605349531	27.12337696395302
55-59	27.770072112769885	22.332329625142133	22.424740182246644	27.472858079841338
60-64	27.71943843505112	22.66694762487982	22.57551651087307	27.038097429195997
65-69	27.648110444205294	22.372898058037265	22.592685464875895	27.386306032881553
70-74	27.361442563669662	22.44181089786741	22.910701970215623	27.28604456824731
75-79	27.978793939943102	22.156898443818733	22.892328611429	26.97197900480916
80-84	27.44518046600377	22.134361421893306	22.829216335155024	27.5912417769479
85-89	27.965020845116396	22.581168443579475	22.54389474561229	26.909915965691845
90-94	27.645903937834	22.16101057530162	22.958504105617305	27.234581381247068
95-99	27.801282937232596	22.43057006732292	22.332060971961237	27.43608602348325
100-104	27.704309292904895	22.703776833137827	22.313849545624358	27.278064328332924
105-109	28.316578757752804	22.546931440276445	22.373720085475792	26.76276971649496
110-114	28.106850298383463	23.18603088511793	22.130630927069113	26.576487889429494
115-119	29.093799767407365	23.25445007362193	21.897512686765445	25.75423747220526
120-124	29.276369756772148	23.60876318511855	21.79378235516036	25.321084702948944
125-129	30.10340797163248	23.670261839494845	21.3958809617442	24.830449227128472
130-134	29.835191917134253	23.790001118979408	21.773452501810596	24.601354462075744
135-139	30.42057533893829	24.33245642588623	21.659632961925475	23.587335273250005
140-144	31.11063196817086	24.329073189884003	21.565164043977155	22.995130797967985
145-149	31.09304802296695	24.462705701871066	21.865276211538916	22.578970063623068
150-151	30.82711324122613	24.79297079945266	21.829816506653515	22.550099452667695
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	211529.0
1	133285.5
2	45619.0
3	31769.5
4	24302.0
5	19955.0
6	29464.5
7	34000.5
8	22844.0
9	15755.0
10	12468.0
11	11124.5
12	10326.0
13	9321.5
14	8963.0
15	9236.0
16	9554.0
17	10333.5
18	12147.0
19	14420.0
20	18258.5
21	24971.5
22	33276.0
23	45370.0
24	64135.5
25	93572.0
26	144046.5
27	256479.0
28	300429.5
29	283404.0
30	369156.0
31	435489.0
32	466527.5
33	523365.5
34	563144.5
35	622533.5
36	675510.5
37	677332.5
38	644453.5
39	634372.0
40	717683.0
41	803922.5
42	874112.0
43	1010788.0
44	1275578.0
45	1486460.5
46	1758508.5
47	2080501.0
48	2300702.0
49	2454632.5
50	2545913.5
51	2511764.5
52	2503802.0
53	2549347.5
54	2478557.5
55	2583075.0
56	2569855.0
57	2344022.0
58	2454771.0
59	2678584.0
60	2432275.0
61	2039774.0
62	1756936.0
63	1403691.5
64	1201131.0
65	1199863.5
66	1146541.5
67	1085001.0
68	999062.0
69	941805.0
70	964409.0
71	923736.5
72	1005090.0
73	954098.5
74	713354.5
75	517777.0
76	499497.5
77	525091.5
78	414065.5
79	410243.5
80	396501.5
81	402039.5
82	502769.0
83	517706.5
84	396322.0
85	286700.0
86	255563.5
87	154064.0
88	31362.0
89	5542.0
90	2264.0
91	1074.0
92	653.5
93	478.0
94	398.5
95	371.5
96	355.5
97	353.0
98	365.5
99	514.0
100	1043.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.382549829021853
2	0.048397877025879846
3	0.015762098217852415
4	0.02622076003542858
5	1.5240563343794138E-4
6	0.022948932675384698
7	0.0035780368437402935
8	0.002034405473873438
9	0.001504481298891972
10-14	0.005508135342802076
15-19	0.005019878029072451
20-24	0.00505567237967806
25-29	0.005829165924796126
30-34	0.0051943754882747915
35-39	0.005146276829648505
40-44	0.005209196586572426
45-49	0.004673399900944728
50-54	0.008482142520072731
55-59	0.00502603018308279
60-64	0.00615886545107747
65-69	0.007391813043422211
70-74	0.008149366916786216
75-79	0.008593440578987043
80-84	0.005898517479094491
85-89	0.009844285346634589
90-94	0.0073828644557708085
95-99	0.00819830450550482
100-104	0.008783038779851126
105-109	0.01112561124096972
110-114	0.009820236017321446
115-119	0.014352416019392484
120-124	0.00816810302218134
125-129	0.014840952976486218
130-134	0.013786697493805412
135-139	0.014554038884913138
140-144	0.0120901012037724
145-149	0.01333367524395318
150-151	0.019986111232678294
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	7.1519666E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.289971212451867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.79880175202467	8.877541523106872
2	11.602565789105244	3.0839553065557515
3	6.623767410155536	2.640890345968318
4	3.975004982942804	2.1131080717065034
5	2.4454009015765497	1.6249653792428098
6	1.561394893142332	1.2450535908678573
7	1.0841733674085345	1.0086042989516497
8	0.7798009577160435	0.8290825823590892
9	0.5709326448634331	0.6828910573035628
>10	3.6506591498670775	9.350017499308235
>50	0.4119962064190525	3.823407298656988
>100	0.3556044714619208	9.784735720757771
>500	0.054490213375028115	5.105997268561762
>1k	0.06669807445250465	19.208849302525845
>5k	0.011234203241606474	10.469182583225159
>10k+	0.007474982247895714	20.15171817090188
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	222083	0.3105201861541132	No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG	105650	0.14772160708916063	No Hit
CGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGATCAAAAC	97987	0.13700707159342718	No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC	89167	0.1246747992363387	No Hit
CCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGG	85493	0.1195377506377057	No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCT	84010	0.11746419509285738	No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC	81045	0.11331848221998128	No Hit
CTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGC	72582	0.10148537326782259	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7198066892538342E-4	0.0	0.0	0.0	0.0
2	1.9435213805388856E-4	0.0	0.0	2.7964336410631446E-6	0.0
3	2.6705941272153034E-4	0.0	0.0	4.1946504615947175E-6	1.1185734564252578E-5
4	4.292525639031927E-4	0.0	0.0	4.1946504615947175E-6	1.2583951384784152E-5
5	4.7958836944232935E-4	0.0	1.3982168205315723E-6	5.592867282126289E-6	1.2583951384784152E-5
6	5.78861763700071E-4	0.0	1.3982168205315723E-6	6.991084102657862E-6	1.2583951384784152E-5
7	6.809315915988758E-4	0.0	1.3982168205315723E-6	9.787517743721007E-6	1.2583951384784152E-5
8	7.997800213440594E-4	0.0	1.3982168205315723E-6	1.5380385025847297E-5	1.2583951384784152E-5
9	8.571069109858539E-4	0.0	1.3982168205315723E-6	1.817681866691044E-5	1.2583951384784152E-5
10-14	0.0014418411853321573	1.1185734564252578E-6	1.3982168205315723E-6	2.9642196595269332E-5	1.3422881477103096E-5
15-19	0.0033006306265468298	2.2371469128505156E-6	1.3982168205315723E-6	4.4463294892904E-5	1.4261811569422039E-5
20-24	0.00589096710826362	2.7964336410631446E-6	1.3982168205315723E-6	5.39711692725187E-5	1.5660028389953612E-5
25-29	0.009610783137605817	2.7964336410631446E-6	1.3982168205315723E-6	6.124189673928286E-5	1.677860184637887E-5
30-34	0.01748190490710625	2.7964336410631446E-6	1.3982168205315723E-6	6.57161905649839E-5	1.677860184637887E-5
35-39	0.03317213478038335	4.474293825701032E-6	1.3982168205315723E-6	7.270727466764177E-5	1.8176818666910443E-5
40-44	0.041592476116988575	1.1745021292465207E-5	1.3982168205315723E-6	8.193550568315015E-5	2.4049329313143045E-5
45-49	0.048443179250865075	1.817681866691044E-5	1.3982168205315723E-6	9.116373669865852E-5	2.7684693046525138E-5
50-54	0.05744881414854482	1.9295392123335698E-5	1.3982168205315723E-6	1.0374768808344268E-4	3.3557203692757733E-5
55-59	0.06902688835263857	2.376968594903673E-5	1.3982168205315723E-6	1.1213698900663211E-4	3.635363733382088E-5
60-64	0.08659436412916136	2.376968594903673E-5	1.3982168205315723E-6	1.1856878638107733E-4	3.635363733382088E-5
65-69	0.11481513350467829	2.376968594903673E-5	1.3982168205315723E-6	1.3646596168388146E-4	3.691292406203351E-5
70-74	0.16164253339773707	2.4328972677249357E-5	1.3982168205315723E-6	1.5883743081238663E-4	3.915007097488403E-5
75-79	0.2424273066375897	2.6286476225993558E-5	1.3982168205315723E-6	1.7701424947929705E-4	3.915007097488403E-5
80-84	0.38424312552018913	2.7125406318312508E-5	1.3982168205315723E-6	1.915557044128254E-4	4.0548287795415604E-5
85-89	0.6290281053605591	2.7964336410631447E-5	2.5167902769568305E-6	2.5531439142906513E-4	4.1946504615947174E-5
90-94	1.046557739797051	2.7964336410631447E-5	2.7964336410631446E-6	2.8299908447559024E-4	4.3904008164691374E-5
95-99	1.7406060033893336	2.8523623138844077E-5	2.7964336410631446E-6	3.2075093862994267E-4	4.4742938257010313E-5
100-104	2.824129799487598	2.936255323116302E-5	5.592867282126289E-6	3.4004633075327843E-4	4.586151171343557E-5
105-109	4.38686864113711	2.9921839959375647E-5	5.592867282126289E-6	3.6185851315357096E-4	4.614115507754189E-5
110-114	6.47820447036204	3.355720369275774E-5	6.711440738551548E-6	4.071607381387939E-4	4.781901526217978E-5
115-119	9.176585360451766	3.8870427610777705E-5	6.991084102657862E-6	4.616911941395252E-4	6.627547729319653E-5
120-124	12.294373969811323	4.278543470826611E-5	6.991084102657862E-6	5.246109510634461E-4	6.879226757015337E-5
125-129	15.841214079495282	4.725972853396715E-5	9.507874379614691E-6	5.766246167872204E-4	7.074977111889755E-5
130-134	19.55899458479015	5.089509226734924E-5	1.3982168205315723E-5	6.496115348189684E-4	7.382584812406702E-5
135-139	23.44704322304861	5.22933090878808E-5	1.3982168205315723E-5	6.717033605833674E-4	7.830014194976805E-5
140	25.64379565195397	5.732688964179447E-5	1.5380385025847297E-5	6.963119766247231E-4	7.969835877029962E-5
>>END_MODULE
