##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261617_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29868177
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.338331395317496	32.0	32.0	32.0	32.0	32.0
2	31.67855537350003	32.0	32.0	32.0	32.0	32.0
3	35.70914502080257	37.0	37.0	37.0	32.0	37.0
4	36.29806244954287	37.0	37.0	37.0	37.0	37.0
5	36.487912168191585	37.0	37.0	37.0	37.0	37.0
6	40.04583952344999	41.0	41.0	41.0	37.0	41.0
7	40.11322254451619	41.0	41.0	41.0	37.0	41.0
8	40.13587863095896	41.0	41.0	41.0	37.0	41.0
9	40.19235512766648	41.0	41.0	41.0	37.0	41.0
10-14	40.25884783661219	41.0	41.0	41.0	41.0	41.0
15-19	40.25163677716253	41.0	41.0	41.0	41.0	41.0
20-24	40.24472239467444	41.0	41.0	41.0	41.0	41.0
25-29	40.18426761700254	41.0	41.0	41.0	41.0	41.0
30-34	40.16504808445457	41.0	41.0	41.0	41.0	41.0
35-39	40.15191873277033	41.0	41.0	41.0	41.0	41.0
40-44	40.074017460121524	41.0	41.0	41.0	38.6	41.0
45-49	40.03829778429397	41.0	41.0	41.0	37.0	41.0
50-54	40.07156955712429	41.0	41.0	41.0	37.0	41.0
55-59	40.04373190235212	41.0	41.0	41.0	37.0	41.0
60-64	40.004070586564424	41.0	41.0	41.0	37.0	41.0
65-69	39.961439300430015	41.0	41.0	41.0	37.0	41.0
70-74	39.914609117255466	41.0	41.0	41.0	37.0	41.0
75-79	39.49854997176426	41.0	40.2	41.0	36.0	41.0
80-84	39.864305404377376	41.0	41.0	41.0	37.0	41.0
85-89	39.85195116528203	41.0	41.0	41.0	37.0	41.0
90-94	39.805548346656714	41.0	41.0	41.0	37.0	41.0
95-99	39.75754143280991	41.0	41.0	41.0	37.0	41.0
100-104	39.69715758681891	41.0	41.0	41.0	37.0	41.0
105-109	39.594545847240695	41.0	41.0	41.0	37.0	41.0
110-114	39.5045951549035	41.0	41.0	41.0	37.0	41.0
115-119	39.409624732035034	41.0	41.0	41.0	37.0	41.0
120-124	39.28186867916311	41.0	41.0	41.0	37.0	41.0
125-129	39.1257755369536	41.0	41.0	41.0	37.0	41.0
130-134	38.929753041171544	41.0	41.0	41.0	33.0	41.0
135-139	38.704056588388376	41.0	41.0	41.0	32.0	41.0
140-144	38.45981575641527	41.0	41.0	41.0	32.0	41.0
145-149	38.18874380582384	41.0	40.2	41.0	32.0	41.0
150	34.465011473582734	37.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	10.0
13	14.0
14	20.0
15	32.0
16	56.0
17	111.0
18	346.0
19	1016.0
20	2456.0
21	5094.0
22	10056.0
23	19030.0
24	33691.0
25	60585.0
26	118512.0
27	105153.0
28	115747.0
29	149173.0
30	187515.0
31	233084.0
32	283728.0
33	343899.0
34	423502.0
35	526636.0
36	683535.0
37	941554.0
38	1435321.0
39	2854457.0
40	2.1333844E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.65071175880712	10.09422218445797	10.620718922363997	37.63434713437091
2	26.180570239962602	11.593786524410548	37.465045809490185	24.760597426136666
3	25.255026445035462	15.750428959892664	23.95325633700376	35.04128825806811
4	27.438116902519994	24.23382285528115	24.3255314088066	24.002528833392255
5	28.183192584856375	27.571806030057278	26.14422629250787	18.100775092578473
6	22.322983499226737	27.93902111732568	29.15816821221356	20.579827171234026
7	19.64122174541057	21.891082369824574	41.38937110782523	17.07832477693963
8	20.63962322881689	21.16432088106484	34.74963586890269	23.446420021215577
9	20.30414034933121	21.840159499198926	35.83269605681829	22.023004094651576
10-14	25.14249302377643	24.981438889146652	27.43033169394962	22.445736393127305
15-19	25.327417528413566	24.091392424292106	27.31184526027885	23.26934478701548
20-24	24.97016585346752	24.53623569346055	27.29549097498737	23.198107478084562
25-29	24.801627942593576	24.483439991215363	27.34721261498077	23.367719451210295
30-34	24.669608960813946	24.590924905140167	26.871391143358426	23.868074990687465
35-39	24.989204728464202	24.622164826606685	26.756703601794367	23.631926843134746
40-44	24.768326964022123	24.49566768538937	27.267402913839806	23.4686024367487
45-49	24.72364845316273	24.298646041164243	27.352705689174616	23.624999816498413
50-54	24.736354125029163	24.575673288262614	26.862761704493426	23.8252108822148
55-59	24.894492342479758	24.463979542723667	26.80336957287371	23.83815854192287
60-64	24.603420651479606	24.500033266742708	26.91443608960528	23.982109992172404
65-69	24.82782191318509	25.15238679401949	26.641873757854643	23.377917534940778
70-74	24.786193719993268	24.99872757175727	26.601871984511632	23.613206723737832
75-79	24.913721444967734	24.83009338841299	26.397699935823553	23.858485230795726
80-84	24.64985709854429	24.903991479649985	26.56609346423689	23.88005795756883
85-89	25.054975670486897	24.644334196685726	26.525543383946577	23.775146748880807
90-94	25.29051374650551	24.959712812529915	26.10385695058117	23.6459164903834
95-99	25.524344121242017	25.00631740308499	25.915766311591014	23.55357216408198
100-104	25.252437050252585	25.231019482199084	25.73991975603847	23.77662371150986
105-109	25.517745021378264	25.4143157775616	25.227015374839016	23.84092382622111
110-114	25.738756965744813	25.82783543883933	24.897080001665497	23.53632759375036
115-119	25.439337285420393	26.297722339817263	24.430981028604055	23.83195934615829
120-124	25.46385375671732	26.44881262458038	24.06287380391536	24.024459814786944
125-129	24.977106307589004	26.635249322968978	24.006971004371934	24.380673365070084
130-134	24.638211519465482	26.799621434781677	23.937047736359425	24.62511930939342
135-139	24.238446894822403	27.129292442444424	23.705125002192172	24.927135660541
140-144	23.899606830083975	27.35772210788316	23.783202141208264	24.9594689208246
145-149	23.425112082853314	27.349054006841794	24.128309733084734	25.09752417722016
150	23.1009612672377	27.211332650131276	24.225435653471585	25.462270429159435
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	18.5
2	13.5
3	16.5
4	20.0
5	25.0
6	28.0
7	33.5
8	41.0
9	41.5
10	49.5
11	65.0
12	70.0
13	82.0
14	106.0
15	133.0
16	185.5
17	318.5
18	521.0
19	784.5
20	1433.5
21	2591.0
22	3946.0
23	6047.5
24	9784.0
25	15571.0
26	23461.0
27	33930.5
28	51541.5
29	77878.5
30	108545.5
31	143182.0
32	189458.0
33	251417.0
34	326286.0
35	402309.0
36	477413.5
37	607090.0
38	707539.0
39	691820.5
40	675325.5
41	749687.0
42	834417.5
43	927836.0
44	1052671.5
45	1171557.5
46	1231300.0
47	1265909.5
48	1319441.5
49	1389808.0
50	1476785.5
51	1459527.5
52	1414260.5
53	1403602.5
54	1314252.5
55	1222770.5
56	1134549.5
57	1011780.5
58	916486.0
59	808702.5
60	678970.5
61	548689.0
62	438883.5
63	339362.0
64	258476.5
65	195810.0
66	139555.0
67	103688.0
68	76732.5
69	53871.0
70	37909.0
71	25154.0
72	18512.0
73	13192.0
74	8823.0
75	5977.0
76	4085.5
77	2498.5
78	1395.0
79	816.5
80	515.5
81	332.0
82	195.5
83	101.5
84	48.5
85	26.0
86	13.5
87	10.5
88	6.5
89	3.5
90	2.5
91	4.0
92	4.0
93	3.0
94	2.0
95	2.0
96	3.0
97	3.5
98	3.0
99	1.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0199852505226548
2	0.012494903857038211
3	0.0
4	0.020711006232486166
5	1.0044134933310458E-5
6	3.3480449777701532E-6
7	0.0034752706869254192
8	0.0016740224888850766
9	6.361285457763291E-4
10-14	2.7855734215047676E-4
15-19	9.910213134199654E-5
20-24	1.7610716583071006E-4
25-29	0.047708301715233575
30-34	0.001246811949721605
35-39	0.004677218833944904
40-44	0.03874826374572509
45-49	0.10687897021636103
50-54	9.099986249579277E-4
55-59	0.0027413792277982013
60-64	0.0017068333296672241
65-69	0.0016639783539517663
70-74	2.946279580437735E-4
75-79	7.044286633228402E-4
80-84	0.002248547007070435
85-89	0.0014041700636768023
90-94	8.182621925670254E-4
95-99	0.0022860451108214605
100-104	0.0037069553993871137
105-109	7.43265985064974E-4
110-114	0.002691158553131649
115-119	1.4329632504856256E-4
120-124	0.0013793945308413031
125-129	0.0011490490363707166
130-134	1.1450313823973925E-4
135-139	0.0018374070838002598
140-144	0.0016063919803341194
145-149	0.014054423207683549
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.9868177E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.368906655152887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.15469210584226	19.20057219665788
2	13.381106835386081	7.324525278406198
3	5.203587357248019	4.272494899573642
4	2.723145398243918	2.981180488517877
5	1.7203976453958916	2.354270128329249
6	1.1480799164318414	1.8853015239287274
7	0.8223107751877121	1.5754022793347235
8	0.6157774015642365	1.3482523379011349
9	0.4703083765794709	1.1584643451966028
>10	3.137948950378662	16.47723599132881
>50	0.32453061315853277	6.185253557161689
>100	0.24655476885307048	13.47897927221239
>500	0.028297514198714794	5.34887430628697
>1k	0.020870177331925716	10.823562862707904
>5k	0.0019340902039435119	3.5479213208654485
>10k+	4.5807399566863817E-4	2.037709211590803
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG	91940	0.30781925525618786	TruSeq Adapter, Index 13 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.039721439979414E-5	3.3480449777701532E-6	0.0	6.6960899555403065E-6	0.0
2	1.3057375413303598E-4	6.6960899555403065E-6	0.0	1.6740224888850766E-5	3.3480449777701532E-6
3	1.8079442879958828E-4	6.6960899555403065E-6	0.0	2.008826986662092E-5	2.008826986662092E-5
4	3.080201379548541E-4	6.6960899555403065E-6	0.0	2.008826986662092E-5	2.6784359822161226E-5
5	3.950693073768781E-4	6.6960899555403065E-6	0.0	2.343631484439107E-5	4.017653973324184E-5
6	4.921626117322125E-4	6.6960899555403065E-6	0.0	2.343631484439107E-5	4.352458471101199E-5
7	5.390352414209946E-4	6.6960899555403065E-6	0.0	2.343631484439107E-5	4.352458471101199E-5
8	5.792117811542365E-4	1.004413493331046E-5	0.0	2.6784359822161226E-5	4.352458471101199E-5
9	7.600062099538248E-4	1.004413493331046E-5	0.0	2.6784359822161226E-5	4.352458471101199E-5
10-11	0.0010027394708421607	1.004413493331046E-5	0.0	3.0132404799931377E-5	4.687262968878214E-5
12-13	0.0012588649116415775	1.004413493331046E-5	0.0	3.348044977770153E-5	5.6916764622092606E-5
14-15	0.0014095269356412345	1.004413493331046E-5	0.0	3.348044977770153E-5	6.0264809599862754E-5
16-17	0.0015116423074632243	1.004413493331046E-5	0.0	3.6828494755471686E-5	6.0264809599862754E-5
18-19	0.0016304979041740645	1.004413493331046E-5	0.0	3.6828494755471686E-5	6.0264809599862754E-5
20-21	0.001725917186040514	1.004413493331046E-5	0.0	3.6828494755471686E-5	6.0264809599862754E-5
22-23	0.0017845079731514915	1.3392179911080613E-5	0.0	4.352458471101199E-5	6.0264809599862754E-5
24-25	0.0018648610526179753	1.3392179911080613E-5	0.0	5.189469715543737E-5	6.0264809599862754E-5
26-27	0.0019619543569733095	1.5066202399965688E-5	3.3480449777701532E-6	5.6916764622092606E-5	6.0264809599862754E-5
28-29	0.0020690917962619547	1.6740224888850766E-5	3.3480449777701532E-6	6.361285457763292E-5	6.528687706651799E-5
30-31	0.002253234270039313	1.6740224888850766E-5	3.3480449777701532E-6	6.863492204428815E-5	7.030894453317321E-5
32-33	0.00251605580079427	1.6740224888850766E-5	5.02206746665523E-6	7.365698951094337E-5	7.86790569775986E-5
34-35	0.002901080973237838	1.6740224888850766E-5	6.6960899555403065E-6	7.365698951094337E-5	9.541928186644936E-5
36-37	0.0033731553151034295	1.6740224888850766E-5	6.6960899555403065E-6	8.370112444425383E-5	1.1383352924418521E-4
38-39	0.004076244760435162	1.6740224888850766E-5	6.6960899555403065E-6	8.704916942202398E-5	1.6238018142185242E-4
40-41	0.004873079465144458	1.6740224888850766E-5	6.6960899555403065E-6	9.709330435533445E-5	1.8581649626624348E-4
42-43	0.0058574046886088835	1.8414247377735843E-5	6.6960899555403065E-6	1.004413493331046E-4	1.9586063119955395E-4
44-45	0.007149750050028162	2.008826986662092E-5	1.1718157422195537E-5	1.004413493331046E-4	2.0925281111063457E-4
46-47	0.008791966111624423	2.343631484439107E-5	1.6740224888850766E-5	1.0211537182198967E-4	2.1762292355505995E-4
48-49	0.010603258444598074	2.343631484439107E-5	1.6740224888850766E-5	1.0378939431087474E-4	2.209709685328301E-4
50-51	0.012978696356325998	2.343631484439107E-5	1.6740224888850766E-5	1.071374392886449E-4	2.4273326088833608E-4
52-53	0.016095726230630013	2.343631484439107E-5	1.6740224888850766E-5	1.071374392886449E-4	2.494293508438764E-4
54-55	0.019922541640221295	3.850251724435676E-5	1.6740224888850766E-5	1.1215950675530012E-4	2.5277739582164655E-4
56-57	0.024844167757543424	4.687262968878214E-5	1.6740224888850766E-5	1.1550755173307029E-4	2.561254407994167E-4
58-59	0.030793643683040984	4.687262968878214E-5	1.6740224888850766E-5	1.1885559671084043E-4	2.577994632883018E-4
60-61	0.038325070860534945	4.687262968878214E-5	1.6740224888850766E-5	1.2052961919972551E-4	2.6114750826607196E-4
62-63	0.04813316862291261	4.687262968878214E-5	1.6740224888850766E-5	1.2052961919972551E-4	2.6114750826607196E-4
64-65	0.06144666947701562	4.687262968878214E-5	1.6740224888850766E-5	1.2555168666638074E-4	2.7119164319938237E-4
66-67	0.07907747432995324	5.02206746665523E-5	1.6740224888850766E-5	1.3224777662192107E-4	2.8290980062157796E-4
68-69	0.10236647519532244	5.02206746665523E-5	1.6740224888850766E-5	1.3392179911080613E-4	3.06346115465969E-4
70-71	0.13316514094583007	5.02206746665523E-5	1.6740224888850766E-5	1.3392179911080613E-4	3.080201379548541E-4
72-73	0.1741954321484033	5.02206746665523E-5	1.6740224888850766E-5	1.3559582159969119E-4	3.147162279103944E-4
74-75	0.22899623234454516	5.02206746665523E-5	1.6740224888850766E-5	1.3726984408857627E-4	3.2476036284370484E-4
76-77	0.3013006116844694	5.356871964432245E-5	1.6740224888850766E-5	1.3726984408857627E-4	3.297824303103601E-4
78-79	0.39351581450719275	5.356871964432245E-5	1.6740224888850766E-5	1.422919115552315E-4	3.3145645279924514E-4
80-81	0.5119010108986565	5.356871964432245E-5	2.343631484439107E-5	1.439659340441166E-4	3.3145645279924514E-4
82-83	0.6633364332881783	5.356871964432245E-5	3.0132404799931377E-5	1.439659340441166E-4	3.348044977770153E-4
84-85	0.8550521848052528	5.356871964432245E-5	3.0132404799931377E-5	1.506620239996569E-4	3.3647852026590043E-4
86-87	1.099516384947096	5.356871964432245E-5	3.0132404799931377E-5	1.573581139551972E-4	3.381525427547855E-4
88-89	1.3953965118125555	6.0264809599862754E-5	3.0132404799931377E-5	1.6070615893296736E-4	3.381525427547855E-4
90-91	1.7494556162567272	6.361285457763292E-5	3.348044977770153E-5	1.7409833884404795E-4	3.381525427547855E-4
92-93	2.172979957899674	6.696089955540306E-5	3.348044977770153E-5	1.791204063107032E-4	3.415005877325556E-4
94-95	2.6717800688003157	7.030894453317321E-5	3.348044977770153E-5	1.8246845128847336E-4	3.515447226658661E-4
96-97	3.2473307627713606	7.365698951094337E-5	3.6828494755471686E-5	1.8916454124401366E-4	3.515447226658661E-4
98-99	3.905531295063639	8.035307946648368E-5	3.6828494755471686E-5	1.941866087106689E-4	3.515447226658661E-4
100-101	4.648115952975637	8.035307946648368E-5	3.6828494755471686E-5	1.941866087106689E-4	3.515447226658661E-4
102-103	5.474497154613755	8.035307946648368E-5	3.6828494755471686E-5	1.9586063119955395E-4	3.515447226658661E-4
104-105	6.396829977269787	8.035307946648368E-5	3.6828494755471686E-5	2.0255672115509425E-4	3.515447226658661E-4
106-107	7.398899839116394	8.035307946648368E-5	3.6828494755471686E-5	2.1762292355505995E-4	3.515447226658661E-4
108-109	8.487196925342982	8.035307946648368E-5	3.6828494755471686E-5	2.2431901351060025E-4	3.515447226658661E-4
110-111	9.635504369751125	8.035307946648368E-5	3.6828494755471686E-5	2.2599303599948534E-4	3.565667901325213E-4
112-113	10.848932962999381	8.035307946648368E-5	3.6828494755471686E-5	2.3603717093279578E-4	3.599148351102915E-4
114-115	12.127599886661981	8.035307946648368E-5	3.6828494755471686E-5	2.3938521591056596E-4	3.649369025769467E-4
116-117	13.472583211221764	8.035307946648368E-5	3.6828494755471686E-5	2.4105923839945102E-4	3.7498103751025714E-4
118-119	14.870192780764626	8.370112444425383E-5	3.6828494755471686E-5	2.4105923839945102E-4	3.7498103751025714E-4
120-121	16.2861898133254	8.370112444425383E-5	3.6828494755471686E-5	2.4105923839945102E-4	3.7498103751025714E-4
122-123	17.717815519842404	8.370112444425383E-5	3.6828494755471686E-5	2.4105923839945102E-4	3.766550599991422E-4
124-125	19.173167147094382	8.370112444425383E-5	4.017653973324184E-5	2.444072833772212E-4	3.9674332986576314E-4
126-127	20.643502949644365	8.370112444425383E-5	4.1850562222126914E-5	2.477553283549913E-4	4.1013550977684373E-4
128-129	22.13549223308808	8.370112444425383E-5	4.352458471101199E-5	2.477553283549913E-4	4.1180953226572885E-4
130-131	23.63437514114102	8.370112444425383E-5	4.352458471101199E-5	2.511033733327615E-4	4.1180953226572885E-4
132-133	25.12410449422474	8.704916942202398E-5	4.352458471101199E-5	2.511033733327615E-4	4.1180953226572885E-4
134-135	26.60779564819105	8.872319191090906E-5	4.352458471101199E-5	2.5445141831053166E-4	4.2520171217680944E-4
136-137	28.06393574003529	9.374525937756429E-5	4.352458471101199E-5	2.561254407994167E-4	4.452899820434304E-4
138-139	29.50562566975547	9.541928186644936E-5	4.519860719989706E-5	2.611475082660719E-4	4.519860719989707E-4
>>END_MODULE
