##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261616_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31225712
Sequences flagged as poor quality	0
Sequence length	150
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.769017340581378	32.0	32.0	32.0	32.0	32.0
2	31.33483838575082	32.0	32.0	32.0	32.0	32.0
3	35.02262747443517	37.0	32.0	37.0	32.0	37.0
4	35.61754809626118	37.0	37.0	37.0	32.0	37.0
5	35.83838770433802	37.0	37.0	37.0	37.0	37.0
6	39.17938956844283	41.0	41.0	41.0	37.0	41.0
7	39.31656194100554	41.0	41.0	41.0	37.0	41.0
8	39.35218204151758	41.0	41.0	41.0	37.0	41.0
9	39.38803406628486	41.0	41.0	41.0	37.0	41.0
10-14	39.39470991726306	41.0	41.0	41.0	37.0	41.0
15-19	39.35222681871913	41.0	41.0	41.0	37.0	41.0
20-24	39.31186874457819	41.0	41.0	41.0	37.0	41.0
25-29	39.27712083554732	41.0	41.0	41.0	37.0	41.0
30-34	39.254018937982906	41.0	41.0	41.0	37.0	41.0
35-39	39.16817955664229	41.0	41.0	41.0	37.0	41.0
40-44	39.11210164239009	41.0	41.0	41.0	37.0	41.0
45-49	39.03896263438285	41.0	41.0	41.0	37.0	41.0
50-54	38.95481717758749	41.0	41.0	41.0	37.0	41.0
55-59	38.899535446941925	41.0	41.0	41.0	33.0	41.0
60-64	38.84344410145075	41.0	41.0	41.0	32.0	41.0
65-69	38.78113446380342	41.0	41.0	41.0	32.0	41.0
70-74	38.72184946175126	41.0	41.0	41.0	32.0	41.0
75-79	38.25184839980591	41.0	39.4	41.0	31.0	41.0
80-84	38.75724784754308	41.0	41.0	41.0	32.0	41.0
85-89	38.73031080924592	41.0	41.0	41.0	32.0	41.0
90-94	38.56909630115079	41.0	41.0	41.0	32.0	41.0
95-99	38.41437998915766	41.0	41.0	41.0	32.0	41.0
100-104	38.22125356821327	41.0	41.0	41.0	32.0	41.0
105-109	38.02561127829527	41.0	37.8	41.0	32.0	41.0
110-114	37.751951763341694	41.0	37.0	41.0	31.0	41.0
115-119	37.42773173594889	41.0	37.0	41.0	27.0	41.0
120-124	37.13996534650675	41.0	37.0	41.0	27.0	41.0
125-129	36.734995487052466	41.0	37.0	41.0	26.0	41.0
130-134	36.14744641211064	41.0	37.0	41.0	22.0	41.0
135-139	35.857231047285644	41.0	36.0	41.0	22.0	41.0
140-144	35.44832842242316	41.0	32.0	41.0	22.0	41.0
145-149	35.104228425600034	41.0	32.0	41.0	22.0	41.0
150	29.40371944120922	32.0	22.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	13.0
12	112.0
13	577.0
14	5392.0
15	29907.0
16	70868.0
17	94638.0
18	101030.0
19	102533.0
20	108635.0
21	117274.0
22	130006.0
23	145204.0
24	165795.0
25	195021.0
26	245227.0
27	282656.0
28	262764.0
29	291297.0
30	333400.0
31	386697.0
32	454635.0
33	544318.0
34	663311.0
35	831807.0
36	1090765.0
37	1523978.0
38	2416944.0
39	5198463.0
40	1.5432445E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.25988452330198	20.562384398988865	12.3420673149426	37.83566376276654
2	25.427523069773194	26.323185334251075	29.528393653238034	18.7208979427377
3	21.15970488556466	26.943436954994144	25.745598592846086	26.151259566595108
4	24.06156728015045	33.31009381516999	21.298874283931678	21.329464620747878
5	26.26978369620523	34.81738062530007	19.948358583464806	18.964477095029892
6	21.291056549807415	34.31212713420273	21.72313636915629	22.67367994683356
7	21.460737286031716	20.64894329201603	32.697420352033596	25.19289906991866
8	21.228469354462053	23.59860014375434	26.721042537936633	28.45188796384697
9	21.257065331288523	24.34662178399647	28.300241800731396	26.096071083983606
10-14	24.712414529948333	26.76133744754054	23.322289163976826	25.203958858534303
15-19	24.502677152725933	26.587747302607546	24.13970384406287	24.76987170060366
20-24	24.493977784518954	26.764754594137898	24.224188982067595	24.517078639275557
25-29	24.132766565979942	26.85512613732122	24.297397629416704	24.71470966728213
30-34	23.911693373967076	26.84928430694718	24.367317503227397	24.871704815858344
35-39	23.77181202491233	26.971520582286768	24.278096743305568	24.97857064949534
40-44	23.807362864441	26.579020429923737	24.78163518787007	24.831981517765193
45-49	23.84412160555244	26.632955986731083	24.68857140941135	24.83435099830513
50-54	24.08389025375659	26.73305717318945	24.39196162254657	24.791090950507385
55-59	24.137753902866354	26.58697730070086	24.457110192331136	24.818158604101654
60-64	23.994769855603675	26.4264367744459	24.932066799061026	24.6467265708894
65-69	24.245255828309396	26.296497255281547	24.978383826219456	24.479863090189596
70-74	24.178225821932063	26.77306798705626	24.683724304292042	24.36498188671964
75-79	24.109178302840736	26.828298875412198	24.608538144228817	24.45398467751825
80-84	24.022484636380455	27.0758895100539	24.505213286103302	24.39641256746234
85-89	24.2004061787817	27.03046973677673	24.11043053120309	24.65869355323848
90-94	24.3219487101898	27.138185784925806	24.14912710527754	24.39073839960685
95-99	24.413347666930495	27.164590908954366	24.169284914151593	24.252776509963546
100-104	25.129027380975373	27.59559950360055	23.568492181381984	23.706880934042097
105-109	25.342227346462394	27.995695906412372	23.086649881341323	23.57542686578391
110-114	25.97215010295409	28.054881621005972	22.807620062144647	23.165348213895296
115-119	26.55269776784701	27.913583183636664	22.77967877242349	22.754040276092834
120-124	27.25607536732204	27.999877613601786	22.577717588595203	22.166329430480967
125-129	28.04977120321368	27.934874179541442	22.355353757691702	21.660000859553175
130-134	28.847842434007738	27.86122699987	22.104451572238187	21.186478993884077
135-139	29.033409082819876	27.78919784212504	22.370302780155114	20.807090294899965
140-144	29.43415157354939	27.725368119708527	22.2342036588309	20.606276647911184
145-149	29.98929917369791	27.229918785295126	22.419095777295226	20.361686263711736
150	29.587460530497147	27.31012101541644	22.623041392422667	20.479377061663744
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	80.0
1	74.5
2	72.0
3	88.5
4	109.5
5	126.0
6	162.0
7	180.0
8	213.5
9	265.0
10	285.5
11	332.0
12	404.5
13	459.0
14	547.0
15	691.5
16	798.5
17	979.5
18	1239.0
19	1630.0
20	2421.5
21	3612.0
22	5373.5
23	7902.0
24	11602.0
25	17075.0
26	25808.0
27	37868.5
28	53482.0
29	78664.0
30	106737.0
31	140762.0
32	192520.5
33	276917.0
34	386736.0
35	452342.0
36	507082.0
37	637590.0
38	715837.0
39	705863.0
40	718455.0
41	786342.5
42	860874.0
43	935803.0
44	1017308.0
45	1115307.5
46	1229597.0
47	1298804.5
48	1383154.0
49	1527674.5
50	1628227.5
51	1550513.5
52	1432937.5
53	1392585.0
54	1327698.0
55	1285212.5
56	1235561.5
57	1096534.0
58	956782.5
59	819720.0
60	682906.5
61	562864.5
62	454917.0
63	369087.5
64	286014.0
65	215956.0
66	163024.5
67	124495.0
68	98982.0
69	77653.0
70	58134.5
71	42877.0
72	34008.5
73	25030.5
74	16995.0
75	12101.5
76	8358.5
77	5617.5
78	3742.0
79	2596.0
80	1720.0
81	1191.0
82	849.0
83	552.0
84	371.0
85	270.5
86	220.5
87	168.0
88	132.5
89	120.0
90	100.5
91	83.5
92	78.5
93	63.0
94	48.5
95	48.5
96	47.5
97	42.5
98	39.5
99	37.5
100	100.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.784385252768616
2	0.01320065976397912
3	4.1632357334237885E-4
4	5.284106892422501E-4
5	0.0
6	0.0
7	1.7933938543979397E-4
8	7.045475856563335E-4
9	0.0
10-14	4.0159212382411E-4
15-19	0.0
20-24	8.838869710961275E-5
25-29	4.227285513938001E-5
30-34	3.362613476996137E-4
35-39	2.581206154722749E-4
40-44	0.001272028641012253
45-49	2.517156374208537E-4
50-54	0.0017447160212071386
55-59	4.7653036702573827E-4
60-64	3.567572774641616E-4
65-69	8.02543749843078E-4
70-74	1.9022784812721004E-4
75-79	4.227285513938001E-5
80-84	0.00176329045755626
85-89	4.861378341028701E-4
90-94	3.27294378427624E-4
95-99	1.7357490519351489E-4
100-104	1.6973191836266215E-4
105-109	5.854149938998989E-4
110-114	1.4539300176726153E-4
115-119	3.778937050338516E-5
120-124	6.340928270907001E-5
125-129	5.956629587821729E-4
130-134	9.543417296617607E-5
135-139	2.1072377789175793E-4
140-144	0.0
145-149	2.754140562111122E-4
150	6.725226953992274E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	3.1225712E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.658050029172706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5713524709917	22.555542047695624
2	12.037698270647617	7.381047116352009
3	4.605808301416161	4.236153039887869
4	2.4134502552239576	2.959667146703029
5	1.4772520969576923	2.2644834347114613
6	0.9595975756369928	1.7651634289051057
7	0.7234316417461347	1.552530242573768
8	0.5519725964732163	1.353792278192658
9	0.4185843311350011	1.1549681428828193
>10	2.6948405665612785	15.980621494049238
>50	0.2859675304072688	6.102924818281591
>100	0.21745272632903032	13.298811931260039
>500	0.025113974029803667	5.302959899579589
>1k	0.016076704389651336	9.340098322218324
>5k	0.0010099930161480402	2.0976128058275947
>10k+	3.90965038493943E-4	2.653623850879388
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC	134476	0.43065791422145955	Illumina Single End PCR Primer 1 (96% over 31bp)
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT	69277	0.22185883223415373	No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC	41124	0.131699158693323	No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA	34189	0.10948989730001993	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.163235733423789E-5	0.0	0.0	3.2024890257106066E-6	0.0
2	4.163235733423789E-5	3.2024890257106066E-6	0.0	3.2024890257106066E-6	0.0
3	5.444231343708031E-5	3.2024890257106066E-6	0.0	3.2024890257106066E-6	0.0
4	1.0568213784845002E-4	3.2024890257106066E-6	0.0	6.404978051421213E-6	0.0
5	1.921493415426364E-4	3.2024890257106066E-6	0.0	6.404978051421213E-6	0.0
6	2.4659165497971673E-4	3.2024890257106066E-6	0.0	6.404978051421213E-6	0.0
7	2.5940161108255915E-4	3.2024890257106066E-6	0.0	6.404978051421213E-6	0.0
8	2.722115671854016E-4	3.2024890257106066E-6	0.0	9.60746707713182E-6	0.0
9	4.515509526251955E-4	3.2024890257106066E-6	0.0	9.60746707713182E-6	0.0
10-11	5.732455356021987E-4	3.2024890257106066E-6	0.0	1.1208711589987123E-5	0.0
12-13	7.28566253349163E-4	3.2024890257106066E-6	0.0	1.2809956102842426E-5	3.2024890257106066E-6
14-15	8.438558582747448E-4	3.2024890257106066E-6	0.0	1.2809956102842426E-5	3.2024890257106066E-6
16-17	9.287218174560759E-4	3.2024890257106066E-6	0.0	1.4411200615697731E-5	6.404978051421213E-6
18-19	0.0010279989772531048	3.2024890257106066E-6	0.0	1.6012445128553034E-5	6.404978051421213E-6
20-21	0.0011160674254601464	3.2024890257106066E-6	0.0	1.921493415426364E-5	6.404978051421213E-6
22-23	0.0012233508078214519	3.2024890257106066E-6	0.0	1.921493415426364E-5	6.404978051421213E-6
24-25	0.0013498491243370207	3.2024890257106066E-6	0.0	2.722115671854016E-5	8.006222564276517E-6
26-27	0.0015083723311096957	6.404978051421213E-6	0.0	3.202489025710607E-5	1.2809956102842428E-5
28-29	0.0017165341177808851	6.404978051421213E-6	0.0	3.202489025710607E-5	1.6012445128553034E-5
30-31	0.001974334484350589	8.006222564276517E-6	0.0	3.682862379567198E-5	1.6012445128553034E-5
32-33	0.0023506269448715852	1.2809956102842426E-5	0.0	3.842986830852728E-5	1.6012445128553034E-5
34-35	0.002917467502422363	1.2809956102842426E-5	0.0	4.163235733423789E-5	1.6012445128553034E-5
36-37	0.00361400886551442	1.2809956102842426E-5	0.0	4.323360184709319E-5	1.6012445128553034E-5
38-39	0.004419434855480638	1.2809956102842426E-5	0.0	4.483484635994849E-5	1.6012445128553034E-5
40-41	0.0054058014753995045	1.2809956102842426E-5	0.0	4.96385798985144E-5	1.6012445128553034E-5
42-43	0.006814896646712171	1.4411200615697731E-5	0.0	5.444231343708031E-5	1.6012445128553034E-5
44-45	0.008476988451055975	1.6012445128553034E-5	0.0	5.764480246279092E-5	1.7613689641408337E-5
46-47	0.010411291822585184	1.6012445128553034E-5	0.0	7.045475856563335E-5	1.921493415426364E-5
48-49	0.012705875209506832	1.921493415426364E-5	0.0	7.525849210419926E-5	1.921493415426364E-5
50-51	0.015592919066184943	1.921493415426364E-5	0.0	7.685973661705456E-5	1.921493415426364E-5
52-53	0.01923575033293076	1.921493415426364E-5	0.0	8.326471466847577E-5	1.921493415426364E-5
54-55	0.02354469931702438	1.921493415426364E-5	0.0	8.966969271989699E-5	1.921493415426364E-5
56-57	0.028783971363086934	1.921493415426364E-5	0.0	9.60746707713182E-5	1.921493415426364E-5
58-59	0.03503042620773547	1.921493415426364E-5	0.0	1.0247964882273941E-4	2.0816178667118943E-5
60-61	0.04334729020750591	1.921493415426364E-5	0.0	1.0247964882273941E-4	2.2417423179974246E-5
62-63	0.054051609775943615	1.921493415426364E-5	0.0	1.0247964882273941E-4	2.2417423179974246E-5
64-65	0.06794240592496338	1.921493415426364E-5	0.0	1.0247964882273941E-4	2.2417423179974246E-5
66-67	0.08643197631490357	1.921493415426364E-5	0.0	1.0568213784845002E-4	2.2417423179974246E-5
68-69	0.11079331033348415	2.2417423179974246E-5	0.0	1.0728338236130532E-4	2.5619912205684853E-5
70-71	0.14229459363488653	2.2417423179974246E-5	0.0	1.1048587138701593E-4	3.202489025710607E-5
72-73	0.1839749883045101	2.2417423179974246E-5	0.0	1.1689084943843714E-4	3.202489025710607E-5
74-75	0.23958941272500048	2.2417423179974246E-5	0.0	1.2169458297700306E-4	3.202489025710607E-5
76-77	0.31191122239262314	2.2417423179974246E-5	0.0	1.3130205005413488E-4	3.202489025710607E-5
78-79	0.40434626438622123	2.2417423179974246E-5	0.0	1.3450453907984549E-4	3.202489025710607E-5
80-81	0.5211154192416814	2.2417423179974246E-5	0.0	1.361057835927008E-4	3.202489025710607E-5
82-83	0.6703786289965141	2.2417423179974246E-5	0.0	1.377070281055561E-4	3.202489025710607E-5
84-85	0.8559420518577767	2.2417423179974246E-5	0.0	1.377070281055561E-4	3.362613476996137E-5
86-87	1.0912049019090422	2.7221156718540156E-5	0.0	1.377070281055561E-4	3.5227379282816675E-5
88-89	1.3744858724118125	2.882240123139546E-5	0.0	1.457132506698326E-4	3.5227379282816675E-5
90-91	1.7124333305834627	2.882240123139546E-5	0.0	1.5371947323410912E-4	3.5227379282816675E-5
92-93	2.1181470577836623	3.0423645744250765E-5	0.0	1.5532071774696443E-4	3.5227379282816675E-5
94-95	2.594832745527148	3.202489025710607E-5	0.0	1.5692196225981973E-4	3.5227379282816675E-5
96-97	3.143822629248614	3.202489025710607E-5	0.0	1.5692196225981973E-4	3.5227379282816675E-5
98-99	3.774508008015958	3.362613476996137E-5	0.0	1.5692196225981973E-4	3.5227379282816675E-5
100-101	4.488221117263875	3.5227379282816675E-5	0.0	1.5852320677267503E-4	3.5227379282816675E-5
102-103	5.282217423897332	3.842986830852728E-5	0.0	1.6012445128553033E-4	3.5227379282816675E-5
104-105	6.171170732632134	3.842986830852728E-5	0.0	1.6492818482409624E-4	3.5227379282816675E-5
106-107	7.14121266474244	3.842986830852728E-5	0.0	1.6652942933695155E-4	3.5227379282816675E-5
108-109	8.198082080562326	4.0031112821382584E-5	0.0	1.7293440738837276E-4	3.5227379282816675E-5
110-111	9.316799565691248	4.483484635994849E-5	0.0	1.8094062995264927E-4	3.5227379282816675E-5
112-113	10.502029545395153	4.483484635994849E-5	0.0	1.889468525169258E-4	3.842986830852728E-5
114-115	11.751216433431527	4.80373353856591E-5	0.0	1.889468525169258E-4	4.0031112821382584E-5
116-117	13.058030510241046	4.80373353856591E-5	0.0	1.905480970297811E-4	4.163235733423789E-5
118-119	14.41268016562761	4.80373353856591E-5	0.0	1.921493415426364E-4	4.323360184709319E-5
120-121	15.794328404745423	5.1239824411369705E-5	0.0	1.95351830568347E-4	4.80373353856591E-5
122-123	17.196149122236186	5.1239824411369705E-5	0.0	1.95351830568347E-4	4.80373353856591E-5
124-125	18.617783639329026	5.1239824411369705E-5	0.0	1.969530750812023E-4	4.80373353856591E-5
126-127	20.053909419263203	5.1239824411369705E-5	0.0	2.0175680861976821E-4	4.80373353856591E-5
128-129	21.51760542721972	5.1239824411369705E-5	0.0	2.0495929764547882E-4	4.80373353856591E-5
130-131	22.991621456061594	5.284106892422501E-5	0.0	2.0495929764547882E-4	4.96385798985144E-5
132-133	24.44746976466061	5.444231343708031E-5	0.0	2.0656054215833412E-4	5.1239824411369705E-5
134-135	25.882098380975265	5.444231343708031E-5	0.0	2.0976303118404473E-4	5.1239824411369705E-5
136-137	27.290588281862078	5.444231343708031E-5	0.0	2.1937049826117655E-4	5.1239824411369705E-5
138-139	28.679506171068255	5.6043557949935615E-5	0.0	2.2577547631259776E-4	5.284106892422501E-5
>>END_MODULE
