FastQCFastQC Report
Mon 24 Jun 2019
SRR8261616_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8261616_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31225712
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC1344760.43065791422145955Illumina Single End PCR Primer 1 (96% over 31bp)
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT692770.22185883223415373No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC411240.131699158693323No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA341890.10948989730001993No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)