FastQCFastQC Report
Mon 24 Jun 2019
SRR8261615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8261615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30999879
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG1050630.3389142260845599TruSeq Adapter, Index 13 (97% over 37bp)
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT451980.14580056909254388No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC431460.13918118841689672No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT416950.1345005249859201No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG405050.1306618003250916No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCATC390420.1259424270656024No Hit
GGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCA333420.1075552585221381No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC320620.10342621014746542No Hit
CCCATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTT319250.10298427293861372No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)