FastQCFastQC Report
Mon 24 Jun 2019
SRR8261614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8261614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31426279
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG954950.3038698918188819TruSeq Adapter, Index 13 (97% over 37bp)
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT434290.13819326176032484No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC364850.11609710459198812No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCATC326620.10393212635832579No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)