##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261613_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31356297
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.348659696647214	32.0	32.0	32.0	32.0	32.0
2	31.6621407495917	32.0	32.0	32.0	32.0	32.0
3	35.7714733981503	37.0	37.0	37.0	32.0	37.0
4	36.31502418796454	37.0	37.0	37.0	37.0	37.0
5	36.515332279191	37.0	37.0	37.0	37.0	37.0
6	40.07574146908993	41.0	41.0	41.0	37.0	41.0
7	40.142747372242326	41.0	41.0	41.0	37.0	41.0
8	40.270106734860946	41.0	41.0	41.0	41.0	41.0
9	40.275430673462495	41.0	41.0	41.0	41.0	41.0
10-14	40.296444564229	41.0	41.0	41.0	41.0	41.0
15-19	40.27940375102328	41.0	41.0	41.0	41.0	41.0
20-24	40.263480142441566	41.0	41.0	41.0	41.0	41.0
25-29	40.20173290870411	41.0	41.0	41.0	41.0	41.0
30-34	40.18194888254821	41.0	41.0	41.0	41.0	41.0
35-39	40.16829230824035	41.0	41.0	41.0	41.0	41.0
40-44	40.09960625771595	41.0	41.0	41.0	39.4	41.0
45-49	40.05432940630713	41.0	41.0	41.0	38.6	41.0
50-54	40.08847197103663	41.0	41.0	41.0	37.0	41.0
55-59	40.06102980208409	41.0	41.0	41.0	37.0	41.0
60-64	40.02149698352456	41.0	41.0	41.0	37.0	41.0
65-69	39.9753963741318	41.0	41.0	41.0	37.0	41.0
70-74	39.92930579781152	41.0	41.0	41.0	37.0	41.0
75-79	39.54142872801594	41.0	40.2	41.0	36.0	41.0
80-84	39.90739893170421	41.0	41.0	41.0	37.0	41.0
85-89	39.89307760415714	41.0	41.0	41.0	37.0	41.0
90-94	39.84919634483625	41.0	41.0	41.0	37.0	41.0
95-99	39.79839644968282	41.0	41.0	41.0	37.0	41.0
100-104	39.74070785207832	41.0	41.0	41.0	37.0	41.0
105-109	39.63525096729374	41.0	41.0	41.0	37.0	41.0
110-114	39.545939828290315	41.0	41.0	41.0	37.0	41.0
115-119	39.45078595218051	41.0	41.0	41.0	37.0	41.0
120-124	39.32211368580927	41.0	41.0	41.0	37.0	41.0
125-129	39.16776635327826	41.0	41.0	41.0	37.0	41.0
130-134	38.97265409879235	41.0	41.0	41.0	34.0	41.0
135-139	38.74994264788345	41.0	41.0	41.0	32.0	41.0
140-144	38.50660369111825	41.0	41.0	41.0	32.0	41.0
145-149	38.242038790486006	41.0	41.0	41.0	32.0	41.0
150	34.53331335648466	37.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	12.0
13	28.0
14	28.0
15	31.0
16	68.0
17	151.0
18	370.0
19	997.0
20	2562.0
21	5288.0
22	9935.0
23	18374.0
24	32126.0
25	56290.0
26	105521.0
27	104832.0
28	120699.0
29	155948.0
30	195651.0
31	241638.0
32	294822.0
33	358562.0
34	435068.0
35	543152.0
36	702984.0
37	969749.0
38	1479129.0
39	2953806.0
40	2.2568476E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.116995775148894	9.335264335391134	9.474695281192131	42.07304460826784
2	24.419368027993237	11.665113836619952	40.1303592611138	23.78515887427301
3	24.56570684988728	15.095188057441861	24.237134888727454	36.10197020394341
4	26.959756428639942	24.146835004863025	24.26724077297141	24.62616779352562
5	28.10089010834985	27.09879148668553	26.667924255719626	18.132394149244995
6	22.344979064332758	27.531050621187823	29.183324803946075	20.94064551053334
7	19.232643413767306	21.339987582921253	42.29768452130159	17.129684482009857
8	20.162067409842123	20.812350717020735	35.31416821646374	23.711413656673404
9	19.81909823449536	21.08616451250017	36.608945477781155	22.48579177522331
10-14	24.85930648794846	24.90829136551391	27.41368106381793	22.818721082719705
15-19	25.19824743944024	23.965126871217535	27.237004040480734	23.599621648861486
20-24	24.76028856365406	24.359701666808117	27.32103860351534	23.55897116602248
25-29	24.598903075349707	24.434603489202587	27.311166560308784	23.65532687513892
30-34	24.48401205223636	24.51792783106499	26.80098857801172	24.19707153868693
35-39	24.795512365402903	24.532228115386935	26.805665777094728	23.866593742115434
40-44	24.624234631550852	24.309064168917015	27.307734347318313	23.75896685221382
45-49	24.588264523506666	24.188954410705602	27.356869509449233	23.8659115563385
50-54	24.56304185202871	24.581502738759262	26.923707252216765	23.931748156995265
55-59	24.803330905643474	24.425772424633255	26.777646677450797	23.993249992272478
60-64	24.437520573583996	24.472455801269753	26.851994421283422	24.23802920386283
65-69	24.67584555944217	25.08282635873916	26.610403779156627	23.630924302662038
70-74	24.670334440754853	24.902956998389573	26.548862307381967	23.877846253473603
75-79	24.811325607680836	24.742927339365405	26.407208023636393	24.038539029317366
80-84	24.57052934838692	24.853976853010725	26.588160228007666	23.98733357059469
85-89	24.97712770629962	24.580285533277742	26.53567242247256	23.906914337950077
90-94	25.101701607554872	24.867029140972736	26.186404157014305	23.844865094458093
95-99	25.263131736351557	24.932485315007465	26.097821639785813	23.706561308855164
100-104	25.114919814158092	25.204618710515188	25.778766021009936	23.901695454316783
105-109	25.36295188749626	25.33970279069019	25.311462632635617	23.98588268917793
110-114	25.610888214601896	25.78717650002527	24.90853458548066	23.693400699892177
115-119	25.359965566289734	26.23408605899203	24.47474489391416	23.931203480804076
120-124	25.386959178699886	26.39542503970932	24.087986268189642	24.129629513401156
125-129	24.934065885211744	26.69514052187385	23.908452306231933	24.46234128668247
130-134	24.611341864619014	26.825400017590106	23.842334939794124	24.720923177996752
135-139	24.222611582471313	27.191706620315937	23.5890280777896	24.99665371942315
140-144	23.83319984393317	27.432453162250887	23.702855210382463	25.03149178343348
145-149	23.379409540457722	27.436685361793796	24.001332313916155	25.18257278383233
150	22.957175077146385	27.346051097806605	24.138720844492575	25.55805298055443
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	17.0
2	13.5
3	14.5
4	20.0
5	23.5
6	22.5
7	23.0
8	30.0
9	39.0
10	43.0
11	52.5
12	72.5
13	96.5
14	126.5
15	164.0
16	238.0
17	380.5
18	580.0
19	879.0
20	1682.5
21	3100.0
22	4887.0
23	7408.0
24	11868.0
25	19026.5
26	28710.0
27	40423.0
28	58143.0
29	83868.5
30	112479.0
31	144472.0
32	188488.0
33	246301.5
34	314786.5
35	385708.5
36	457403.0
37	577943.5
38	669510.5
39	667462.0
40	672852.0
41	762700.0
42	861840.0
43	978927.0
44	1115538.5
45	1244033.0
46	1323273.0
47	1362084.0
48	1419485.0
49	1506340.5
50	1630803.0
51	1628697.0
52	1566867.0
53	1546275.5
54	1439801.0
55	1319270.5
56	1210319.0
57	1071456.0
58	963747.5
59	838673.5
60	687243.0
61	547875.5
62	429506.5
63	323014.5
64	240596.0
65	179938.5
66	129315.5
67	96013.5
68	70723.0
69	50606.0
70	35693.0
71	23202.5
72	17250.5
73	12367.5
74	8112.0
75	5419.0
76	3805.0
77	2482.0
78	1382.0
79	788.5
80	517.0
81	367.0
82	242.0
83	133.5
84	68.0
85	37.5
86	24.5
87	13.5
88	6.5
89	9.0
90	7.5
91	5.0
92	3.0
93	3.5
94	5.0
95	2.5
96	1.0
97	1.0
98	2.0
99	3.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0301407720433315
2	0.012322883661932403
3	0.0
4	0.020633813999146648
5	6.378304172842859E-6
6	0.0
7	0.0034953106867178867
8	0.0018465190580380074
9	5.19831790086693E-4
10-14	2.8447236610879147E-4
15-19	8.993408883708429E-5
20-24	1.7285204308404147E-4
25-29	0.04865497989127989
30-34	0.0012444071441216416
35-39	0.004834754563014887
40-44	0.039044788994057555
45-49	0.1086703573448102
50-54	8.719141804276187E-4
55-59	0.0026680446355001677
60-64	0.0017687037471293245
65-69	0.0016519807807663005
70-74	3.016937873754672E-4
75-79	7.653965007411431E-4
80-84	0.002296827332640713
85-89	0.001454891181825456
90-94	8.094067995337587E-4
95-99	0.002386123591060513
100-104	0.00374151322778962
105-109	7.660343311584273E-4
110-114	0.0026744229396730105
115-119	1.524414697309443E-4
120-124	0.0013758002100822046
125-129	0.001194018541156183
130-134	1.2246344011858286E-4
135-139	0.0018752214268158004
140-144	0.0015913868911242933
145-149	0.01443601583439524
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	3.1356297E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.938220707246273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.28319241290427	18.933041492286723
2	13.083846035252744	7.04911064394535
3	5.281860309696738	4.2685175630236465
4	2.7150004655371864	2.925491270436684
5	1.6831137050297806	2.267004423074162
6	1.1676233255850774	1.8872216908523813
7	0.8377379884622824	1.5797019579629046
8	0.612499615766477	1.319971986609672
9	0.4878037110500677	1.182650762707246
>10	3.193365328644925	16.724751663057972
>50	0.34105294493041277	6.418713260378649
>100	0.26142897280911376	14.094959930731365
>500	0.029324089941417806	5.476162285275736
>1k	0.019837458950884842	10.362385632248122
>5k	0.001747533150717021	3.263381780399926
>10k+	5.661022882510791E-4	2.246933657009331
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG	65694	0.20950815716536936	TruSeq Adapter, Index 13 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4351184388896433E-4	0.0	0.0	0.0	0.0
2	1.9134912518528575E-4	3.1891520864214293E-6	0.0	6.378304172842859E-6	3.1891520864214293E-6
3	2.8702368777792865E-4	3.1891520864214293E-6	0.0	6.378304172842859E-6	9.567456259264287E-6
4	4.496704441854215E-4	3.1891520864214293E-6	0.0	9.567456259264287E-6	2.2324064604950003E-5
5	5.676690713830144E-4	3.1891520864214293E-6	0.0	1.2756608345685717E-5	2.2324064604950003E-5
6	7.079917631855572E-4	3.1891520864214293E-6	0.0	1.2756608345685717E-5	2.2324064604950003E-5
7	7.845314132596716E-4	6.378304172842859E-6	0.0	1.5945760432107146E-5	2.2324064604950003E-5
8	8.451253029016787E-4	6.378304172842859E-6	0.0	1.5945760432107146E-5	2.2324064604950003E-5
9	0.0011034466219018144	6.378304172842859E-6	0.0	1.5945760432107146E-5	2.5513216691371435E-5
10-11	0.001396848613852586	6.378304172842859E-6	0.0	1.754033647531786E-5	2.8702368777792863E-5
12-13	0.0017588173756614182	6.378304172842859E-6	0.0	1.9134912518528574E-5	3.348609690742501E-5
14-15	0.0019788688696244965	9.567456259264287E-6	0.0	2.0729488561739287E-5	3.508067295063572E-5
16-17	0.0021526776583344646	1.1162032302475001E-5	0.0	2.2324064604950003E-5	3.508067295063572E-5
18-19	0.002300973230353061	1.2756608345685717E-5	0.0	2.2324064604950003E-5	3.508067295063572E-5
20-21	0.0024109989773346005	1.2756608345685717E-5	0.0	2.2324064604950003E-5	3.508067295063572E-5
22-23	0.0024955115076247683	1.9134912518528574E-5	1.5945760432107147E-6	3.826982503705715E-5	3.508067295063572E-5
24-25	0.0026055372546063076	2.2324064604950003E-5	3.1891520864214293E-6	5.581016151237501E-5	3.508067295063572E-5
26-27	0.0026964280890693186	2.2324064604950003E-5	4.783728129632144E-6	6.059388964200715E-5	3.8269825037057155E-5
28-29	0.0028080484120940686	2.2324064604950003E-5	6.378304172842859E-6	6.378304172842858E-5	4.4648129209900005E-5
30-31	0.003016937873754672	2.2324064604950003E-5	6.378304172842859E-6	6.53776177716393E-5	4.4648129209900005E-5
32-33	0.0033167181698782865	2.2324064604950003E-5	6.378304172842859E-6	6.856676985806073E-5	5.581016151237501E-5
34-35	0.003716956756724176	2.5513216691371435E-5	6.378304172842859E-6	7.335049798769288E-5	7.016134590127145E-5
36-37	0.004236788546810869	2.5513216691371435E-5	6.378304172842859E-6	7.972880216053573E-5	1.0843117093832859E-4
38-39	0.00493042912560753	2.5513216691371435E-5	6.378304172842859E-6	8.610710633337859E-5	1.403226918025429E-4
40-41	0.005786716460811683	2.5513216691371435E-5	6.378304172842859E-6	1.0205286676548574E-4	1.5148472410501788E-4
42-43	0.006820001736812227	2.5513216691371435E-5	6.378304172842859E-6	1.0205286676548574E-4	1.5945760432107146E-4
44-45	0.008073338506775848	3.348609690742501E-5	6.378304172842859E-6	1.0205286676548574E-4	1.6902506058033575E-4
46-47	0.009636023029122347	4.4648129209900005E-5	6.378304172842859E-6	1.0205286676548574E-4	1.8018709288281076E-4
48-49	0.011672296636302431	4.4648129209900005E-5	6.378304172842859E-6	1.0205286676548574E-4	1.865653970556536E-4
50-51	0.014298563379470478	5.102643338274287E-5	6.378304172842859E-6	1.0205286676548574E-4	1.9453827727170718E-4
52-53	0.01753555274718823	5.102643338274287E-5	6.378304172842859E-6	1.0843117093832859E-4	2.0410573353097148E-4
54-55	0.021521992855215014	5.2621009425953585E-5	6.378304172842859E-6	1.1162032302475002E-4	2.072948856173929E-4
56-57	0.026351963690100268	6.378304172842858E-5	6.378304172842859E-6	1.1640405115438215E-4	2.072948856173929E-4
58-59	0.032430487566819516	6.378304172842858E-5	6.378304172842859E-6	1.211877792840143E-4	2.1207861374702503E-4
60-61	0.039953697338687665	6.378304172842858E-5	6.378304172842859E-6	1.2437693137043573E-4	2.296189502223429E-4
62-63	0.049848041686810146	6.378304172842858E-5	6.378304172842859E-6	1.2916065950006788E-4	2.296189502223429E-4
64-65	0.06284543101502069	6.378304172842858E-5	6.378304172842859E-6	1.307552355432786E-4	2.3440267835197505E-4
66-67	0.08026298513501132	6.378304172842858E-5	6.378304172842859E-6	1.307552355432786E-4	2.471592866976608E-4
68-69	0.10380849498906072	6.53776177716393E-5	6.378304172842859E-6	1.3394438762970003E-4	2.5832131900013576E-4
70-71	0.13422184386121871	7.016134590127145E-5	7.972880216053573E-6	1.3713353971612146E-4	2.6629419921618937E-4
72-73	0.1752949335822403	7.016134590127145E-5	9.567456259264287E-6	1.419172678457536E-4	2.6948335130261075E-4
74-75	0.23065383007438667	7.016134590127145E-5	9.567456259264287E-6	1.4510641993217501E-4	2.710779273458215E-4
76-77	0.30265212757743687	7.016134590127145E-5	9.567456259264287E-6	1.4989014806180716E-4	2.7267250338903223E-4
78-79	0.39549153396525105	7.016134590127145E-5	1.1162032302475001E-5	1.4989014806180716E-4	2.742670794322429E-4
80-81	0.5144054478116469	7.016134590127145E-5	1.2756608345685717E-5	1.4989014806180716E-4	2.742670794322429E-4
82-83	0.66547558214543	7.016134590127145E-5	1.2756608345685717E-5	1.5148472410501788E-4	2.742670794322429E-4
84-85	0.8569889486631664	7.016134590127145E-5	1.2756608345685717E-5	1.530793001482286E-4	2.790508075618751E-4
86-87	1.0985130036241206	7.016134590127145E-5	1.2756608345685717E-5	1.626467564074929E-4	2.8702368777792865E-4
88-89	1.3916231881589844	7.016134590127145E-5	1.2756608345685717E-5	1.6902506058033575E-4	2.8702368777792865E-4
90-91	1.7440468177731572	7.335049798769288E-5	1.2756608345685717E-5	1.7221421266675719E-4	2.8702368777792865E-4
92-93	2.1685341225081523	7.65396500741143E-5	1.2756608345685717E-5	1.7221421266675719E-4	2.9021283986435003E-4
94-95	2.6687271140466615	7.65396500741143E-5	1.2756608345685717E-5	1.7221421266675719E-4	2.981857200804036E-4
96-97	3.2471372496567437	7.65396500741143E-5	1.2756608345685717E-5	1.7221421266675719E-4	3.0137487216682507E-4
98-99	3.914795806405329	7.65396500741143E-5	1.2756608345685717E-5	1.7859251683960002E-4	3.0456402425324644E-4
100-101	4.672589368572444	7.65396500741143E-5	1.2756608345685717E-5	1.8178166892602145E-4	3.061586002964572E-4
102-103	5.514758327490009	7.972880216053573E-5	1.2756608345685717E-5	1.8497082101244289E-4	3.1253690446930005E-4
104-105	6.4600596811543145	7.972880216053573E-5	1.2756608345685717E-5	1.9453827727170718E-4	3.1253690446930005E-4
106-107	7.498355115082626	8.610710633337859E-5	1.2756608345685717E-5	1.977274293581286E-4	3.157260565557215E-4
108-109	8.628289239638214	8.610710633337859E-5	1.2756608345685717E-5	1.9932200540133933E-4	3.189152086421429E-4
110-111	9.830342849476136	8.610710633337859E-5	1.2756608345685717E-5	2.072948856173929E-4	3.189152086421429E-4
112-113	11.10829987354693	8.610710633337859E-5	1.2756608345685717E-5	2.072948856173929E-4	3.189152086421429E-4
114-115	12.45909234754346	8.610710633337859E-5	1.2756608345685717E-5	2.1367318979023575E-4	3.189152086421429E-4
116-117	13.873045978611568	8.610710633337859E-5	1.2756608345685717E-5	2.184569179198679E-4	3.252935128149858E-4
118-119	15.341028629751785	8.610710633337859E-5	1.2756608345685717E-5	2.2483522209271076E-4	3.252935128149858E-4
120-121	16.83512884190375	8.610710633337859E-5	1.2756608345685717E-5	2.2642979813592147E-4	3.284826649014072E-4
122-123	18.356743463681312	8.610710633337859E-5	1.2756608345685717E-5	2.2642979813592147E-4	3.284826649014072E-4
124-125	19.91143118717111	8.610710633337859E-5	1.4351184388896432E-5	2.280243741791322E-4	3.3645554511746076E-4
126-127	21.483168117714918	8.610710633337859E-5	1.5945760432107146E-5	2.3121352626555362E-4	3.4123927324709294E-4
128-129	23.0726909494447	8.610710633337859E-5	1.5945760432107146E-5	2.3280810230876434E-4	3.4123927324709294E-4
130-131	24.677319200031814	8.77016823765893E-5	1.5945760432107146E-5	2.3280810230876434E-4	3.4123927324709294E-4
132-133	26.268973979931367	9.089083446301073E-5	1.5945760432107146E-5	2.3280810230876434E-4	3.4442842533351437E-4
134-135	27.85296363279121	1.0045829072227502E-4	1.5945760432107146E-5	2.391864064816072E-4	3.476175774199358E-4
136-137	29.408356158892104	1.0205286676548574E-4	1.5945760432107146E-5	2.423755585680286E-4	3.635633378520429E-4
138-139	30.95404887892215	1.0364744280869645E-4	1.754033647531786E-5	2.471592866976608E-4	3.7950909828415007E-4
>>END_MODULE
