##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261622_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30066635
Sequences flagged as poor quality	0
Sequence length	150
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.87211172783386	32.0	32.0	32.0	32.0	32.0
2	31.36866613107852	32.0	32.0	32.0	32.0	32.0
3	35.0488223906666	37.0	32.0	37.0	32.0	37.0
4	35.68253298049483	37.0	37.0	37.0	32.0	37.0
5	35.878225248685126	37.0	37.0	37.0	37.0	37.0
6	39.218857880171825	41.0	41.0	41.0	37.0	41.0
7	39.36788879101369	41.0	41.0	41.0	37.0	41.0
8	39.399468147998604	41.0	41.0	41.0	37.0	41.0
9	39.436079095648715	41.0	41.0	41.0	37.0	41.0
10-14	39.44975962890427	41.0	41.0	41.0	37.0	41.0
15-19	39.40777997271727	41.0	41.0	41.0	37.0	41.0
20-24	39.37459394441713	41.0	41.0	41.0	37.0	41.0
25-29	39.33675750545414	41.0	41.0	41.0	37.0	41.0
30-34	39.310214202553766	41.0	41.0	41.0	37.0	41.0
35-39	39.2029449388001	41.0	41.0	41.0	37.0	41.0
40-44	39.15411524435641	41.0	41.0	41.0	37.0	41.0
45-49	39.08444438162102	41.0	41.0	41.0	37.0	41.0
50-54	39.008313940020216	41.0	41.0	41.0	37.0	41.0
55-59	38.95454915390432	41.0	41.0	41.0	37.0	41.0
60-64	38.901563184573206	41.0	41.0	41.0	35.0	41.0
65-69	38.84141077310447	41.0	41.0	41.0	32.0	41.0
70-74	38.7826731591347	41.0	41.0	41.0	32.0	41.0
75-79	38.30033227196858	41.0	39.4	41.0	31.0	41.0
80-84	38.79800328171077	41.0	41.0	41.0	32.0	41.0
85-89	38.77080253909359	41.0	41.0	41.0	32.0	41.0
90-94	38.608708031344385	41.0	41.0	41.0	32.0	41.0
95-99	38.45391014325348	41.0	41.0	41.0	32.0	41.0
100-104	38.25686686255379	41.0	41.0	41.0	32.0	41.0
105-109	38.06092729033362	41.0	37.8	41.0	32.0	41.0
110-114	37.78508021931952	41.0	37.0	41.0	32.0	41.0
115-119	37.457876473373226	41.0	37.0	41.0	27.0	41.0
120-124	37.16607237224917	41.0	37.0	41.0	27.0	41.0
125-129	36.7577716229302	41.0	37.0	41.0	26.0	41.0
130-134	36.16633800224069	41.0	37.0	41.0	22.0	41.0
135-139	35.86327054557319	41.0	36.0	41.0	22.0	41.0
140-144	35.44744513644443	41.0	32.0	41.0	22.0	41.0
145-149	35.09378259323	41.0	32.0	41.0	22.0	41.0
150	29.234216732268177	32.0	22.0	37.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	14.0
12	111.0
13	630.0
14	5954.0
15	29409.0
16	66386.0
17	86778.0
18	90571.0
19	91994.0
20	97497.0
21	106110.0
22	117391.0
23	132327.0
24	153755.0
25	183289.0
26	235675.0
27	267615.0
28	243610.0
29	271954.0
30	313589.0
31	366598.0
32	432780.0
33	519545.0
34	638101.0
35	806212.0
36	1062576.0
37	1490570.0
38	2364983.0
39	5106104.0
40	1.4784507E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	27.916268296537666	20.379699772760976	13.059540498533359	38.644491432167996
2	24.980625401205046	26.044926050537683	29.965307676079995	19.009140872177277
3	21.17093430871835	26.626217643125973	25.5598808587076	26.64296718944808
4	23.974876195958647	33.12882531655033	21.289808386526037	21.606490100964976
5	26.32138581520679	34.5910475182873	20.002454548039715	19.0851121184662
6	21.346848425172954	33.66625497000246	22.064058714917714	22.92283788990687
7	21.634138350253618	20.635670716446466	32.43859218674506	25.291598746554854
8	21.17151332666587	23.38277167111072	26.85208177497746	28.593633227245952
9	21.330644416975826	24.433894913747416	28.2834410967506	25.952019572526158
10-14	24.772317082076192	26.518656966162762	23.32048962867878	25.388536323082267
15-19	24.63305587738701	26.59628854376288	23.954157157926055	24.816498420924056
20-24	24.56108212871554	26.77596609479453	24.109081636102545	24.553870140387385
25-29	24.23407125682426	26.753294068086532	24.221626227249608	24.7910084478396
30-34	24.097851431983443	26.574924956915048	24.303658369224795	25.023565241876717
35-39	23.724271579358806	26.789557806354676	24.51237780609126	24.97379280819526
40-44	23.98241043365825	26.49275018922391	24.652465109280566	24.872374267837277
45-49	23.974834869847847	26.52682584246213	24.54607355397756	24.952265733712466
50-54	24.247239388669612	26.524716421433432	24.34863793264232	24.879406257254637
55-59	24.250955095118577	26.396650771424813	24.347616886788533	25.00477724666808
60-64	24.066383201888133	26.393269564068632	24.756968564571586	24.78337866947165
65-69	24.27350614855727	26.336918051868597	24.807174534235518	24.582401265338618
70-74	24.254073159368144	26.84855294423862	24.47213657654238	24.42523731985086
75-79	24.19586848194197	26.934640217671262	24.361507963115514	24.507983337271256
80-84	24.164463243617444	27.12916721019136	24.29357544168186	24.412794104509338
85-89	24.40897155119718	27.017508968556502	23.88808531427967	24.68543416596665
90-94	24.540326113026243	27.1703313925176	23.924594325023072	24.364748169433092
95-99	24.708870936064926	27.285871798051918	23.786172015113525	24.219085250769634
100-104	25.418229561668422	27.621784712250236	23.267756593766624	23.69222913231472
105-109	25.750738449172623	27.979603688982756	22.86047102737075	23.409186834473868
110-114	26.36584619834636	27.958609092957033	22.625502483082183	23.050042225614426
115-119	26.991036200617074	27.79221490023144	22.61743067037094	22.59931822878055
120-124	27.620665252349504	27.735004685063082	22.54008312527978	22.10424693730764
125-129	28.289367305827668	27.597317020740537	22.516687766148756	21.596627907283043
130-134	28.954664380506827	27.314364164111932	22.558214402771064	21.172757052610176
135-139	29.066320740907997	27.09443748599566	23.017394037703074	20.82184773539327
140-144	29.242588670132193	26.828693001395067	23.203552376246957	20.725165952225783
145-149	29.55474207866409	26.230670754354236	23.706439092398725	20.508148074582948
150	29.09908021910147	26.192813777486172	24.08648102977465	20.621624973637704
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	64.0
2	73.0
3	91.0
4	113.0
5	121.5
6	147.5
7	188.0
8	200.5
9	229.0
10	282.0
11	329.0
12	395.0
13	461.5
14	526.0
15	622.0
16	754.5
17	951.5
18	1219.0
19	1616.0
20	2367.5
21	3449.5
22	5181.5
23	7576.0
24	11172.0
25	16597.0
26	24721.5
27	36603.0
28	52457.5
29	77740.0
30	104463.5
31	134923.5
32	182402.5
33	252857.0
34	338649.5
35	397358.5
36	449055.5
37	549255.5
38	615718.5
39	626624.5
40	655971.0
41	730051.5
42	818455.0
43	907761.0
44	989678.5
45	1081927.5
46	1192869.0
47	1259715.5
48	1322323.0
49	1449642.0
50	1589089.0
51	1567374.0
52	1475555.0
53	1435419.5
54	1340491.5
55	1263997.5
56	1193016.0
57	1055000.0
58	926974.5
59	795757.5
60	661428.0
61	540633.5
62	434987.0
63	357144.0
64	277591.0
65	203701.5
66	151647.0
67	115975.5
68	93495.5
69	74252.5
70	54880.5
71	39991.0
72	32397.0
73	24692.5
74	16987.5
75	11926.0
76	8180.5
77	5475.5
78	3586.5
79	2524.5
80	1762.0
81	1195.5
82	877.0
83	607.5
84	413.5
85	280.5
86	224.5
87	186.0
88	146.0
89	120.0
90	87.0
91	72.0
92	67.0
93	61.0
94	63.0
95	57.0
96	49.0
97	40.0
98	34.0
99	31.0
100	59.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.7858200626707978
2	0.013559881243777364
3	4.955659321370682E-4
4	5.620848492024464E-4
5	0.0
6	0.0
7	2.0620864290267268E-4
8	7.483378169855057E-4
9	0.0
10-14	4.263862583890748E-4
15-19	0.0
20-24	1.0177394311002877E-4
25-29	4.3237296092495885E-5
30-34	3.565413954704276E-4
35-39	2.6274972240824423E-4
40-44	0.0013370302330141036
45-49	2.620845332375904E-4
50-54	0.0017388044920889883
55-59	5.095349047207977E-4
60-64	3.758318814193873E-4
65-69	8.261649499519982E-4
70-74	2.0088712953744242E-4
75-79	3.658540438595806E-5
80-84	0.001786032923205407
85-89	4.8758366208922284E-4
90-94	3.3991166620408303E-4
95-99	1.862529677830592E-4
100-104	1.6363653598083056E-4
105-109	5.933487402231743E-4
110-114	1.6829286017540707E-4
115-119	3.12638910207278E-5
120-124	5.7871457846879105E-5
125-129	5.926835510525206E-4
130-134	9.71176189154523E-5
135-139	2.2616431802228616E-4
140-144	0.0
145-149	2.793794516745888E-4
150	5.9867025358840456E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	3.0066635E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.599355916007948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.69469215024161	22.5501011422588
2	12.091429998281384	7.399799401018149
3	4.645177321863163	4.264183024939787
4	2.396400589483311	2.9331325821972434
5	1.4287769767281813	2.1859827617751715
6	0.9506407996131547	1.7453397705384788
7	0.6864551760686728	1.4703560377037854
8	0.518489321433161	1.2692351428146194
9	0.39963474577641755	1.100570924017436
>10	2.6389175915459795	15.578240358561967
>50	0.28182866093522757	6.022938996360469
>100	0.22139961298631244	13.794070994947988
>500	0.02654157365911873	5.599391066943529
>1k	0.01851062636884432	10.689644841171493
>5k	8.793744001934308E-4	1.8276490217007484
>10k+	2.2548061543206042E-4	1.5693639330502385
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC	150013	0.49893511528642964	Illumina Single End PCR Primer 1 (96% over 31bp)
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT	40509	0.13473074057007045	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6607566826151315E-5	0.0	0.0	0.0	0.0
2	3.325945853268914E-5	0.0	0.0	3.3259458532689143E-6	0.0
3	5.321513365230263E-5	0.0	0.0	3.3259458532689143E-6	0.0
4	1.3303783413075657E-4	0.0	0.0	3.3259458532689143E-6	0.0
5	2.2283837216901725E-4	0.0	0.0	3.3259458532689143E-6	0.0
6	3.0931296435400904E-4	0.0	0.0	3.3259458532689143E-6	0.0
7	3.392464770334292E-4	0.0	0.0	3.3259458532689143E-6	0.0
8	3.791578272726562E-4	0.0	0.0	6.651891706537829E-6	0.0
9	6.419075496809005E-4	0.0	0.0	9.977837559806742E-6	0.0
10-11	8.464532196569387E-4	0.0	0.0	9.977837559806742E-6	0.0
12-13	0.0010792694293857625	0.0	0.0	9.977837559806742E-6	3.3259458532689143E-6
14-15	0.0012056553718099814	0.0	0.0	1.3303783413075657E-5	3.3259458532689143E-6
16-17	0.0013087596932613178	0.0	0.0	2.161864804624794E-5	3.3259458532689143E-6
18-19	0.0014151899605659229	0.0	0.0	2.6607566826151315E-5	3.3259458532689143E-6
20-21	0.001506653471530818	0.0	0.0	2.9933512679420228E-5	3.3259458532689143E-6
22-23	0.0016213986034685959	0.0	0.0	2.9933512679420228E-5	3.3259458532689143E-6
24-25	0.0017494475188194489	0.0	0.0	2.9933512679420228E-5	4.988918779903372E-6
26-27	0.00189911508221655	0.0	0.0	3.159648560605468E-5	9.977837559806744E-6
28-29	0.0020903569687795126	0.0	0.0	3.658540438595806E-5	1.662972926634457E-5
30-31	0.002319847232655068	0.0	0.0	3.658540438595806E-5	1.662972926634457E-5
32-33	0.002657430736761862	0.0	0.0	3.658540438595806E-5	1.9955675119613484E-5
34-35	0.003174615316945179	3.3259458532689143E-6	0.0	3.658540438595806E-5	1.9955675119613484E-5
36-37	0.003816522866626079	3.3259458532689143E-6	0.0	3.658540438595806E-5	1.9955675119613484E-5
38-39	0.0046663020321362865	3.3259458532689143E-6	0.0	3.658540438595806E-5	1.9955675119613484E-5
40-41	0.005632489302510906	3.3259458532689143E-6	0.0	3.658540438595806E-5	1.9955675119613484E-5
42-43	0.006941248995772224	6.651891706537829E-6	0.0	3.824837731259251E-5	1.9955675119613484E-5
44-45	0.008600895976553412	9.977837559806742E-6	0.0	4.3237296092495885E-5	1.9955675119613484E-5
46-47	0.010707882674599269	9.977837559806742E-6	0.0	4.6563241945764795E-5	1.9955675119613484E-5
48-49	0.013185712335284611	9.977837559806742E-6	0.0	4.988918779903371E-5	1.9955675119613484E-5
50-51	0.01628216792467797	9.977837559806742E-6	0.0	4.988918779903371E-5	1.9955675119613484E-5
52-53	0.020037160793018573	9.977837559806742E-6	0.0	5.155216072566817E-5	1.9955675119613484E-5
54-55	0.024723418500274475	1.3303783413075657E-5	0.0	5.8204052432206E-5	2.3281620972882398E-5
56-57	0.030580409147881033	1.3303783413075657E-5	0.0	5.9867025358840456E-5	2.6607566826151315E-5
58-59	0.038040505696763205	1.3303783413075657E-5	0.0	6.319297121210937E-5	2.9933512679420228E-5
60-61	0.04732654651909002	1.3303783413075657E-5	0.0	6.651891706537828E-5	2.9933512679420228E-5
62-63	0.05982711400860123	1.3303783413075657E-5	0.0	7.317080877191612E-5	2.9933512679420228E-5
64-65	0.07620240841717073	1.3303783413075657E-5	0.0	7.649675462518502E-5	2.9933512679420228E-5
66-67	0.09833491509774872	1.4966756339710114E-5	0.0	7.649675462518502E-5	2.9933512679420228E-5
68-69	0.12735212969459336	1.662972926634457E-5	0.0	7.649675462518502E-5	2.9933512679420228E-5
70-71	0.16514651539821468	1.662972926634457E-5	0.0	8.314864633172285E-5	2.9933512679420228E-5
72-73	0.21637107045733583	1.662972926634457E-5	0.0	8.647459218499177E-5	2.9933512679420228E-5
74-75	0.2843218072125464	1.662972926634457E-5	0.0	8.980053803826069E-5	3.325945853268914E-5
76-77	0.373162809872139	2.3281620972882398E-5	0.0	9.146351096489514E-5	3.325945853268914E-5
78-79	0.4857344361948053	2.3281620972882398E-5	0.0	9.645242974479851E-5	3.325945853268914E-5
80-81	0.6272234987387182	2.3281620972882398E-5	0.0	9.811540267143297E-5	3.325945853268914E-5
82-83	0.8072935331805504	2.3281620972882398E-5	0.0	9.977837559806742E-5	3.658540438595806E-5
84-85	1.0296263615798709	2.3281620972882398E-5	0.0	9.977837559806742E-5	3.658540438595806E-5
86-87	1.3074509335680564	2.3281620972882398E-5	0.0	1.0310432145133634E-4	4.323729609249588E-5
88-89	1.6430505109733762	2.3281620972882398E-5	0.0	1.0975621315787416E-4	4.6563241945764795E-5
90-91	2.037841946729323	2.3281620972882398E-5	0.0	1.16408104864412E-4	4.6563241945764795E-5
92-93	2.504325808325408	2.6607566826151315E-5	0.0	1.1973405071768091E-4	5.155216072566817E-5
94-95	3.0490026569318447	2.8270539752785773E-5	0.0	1.2305999657094983E-4	5.321513365230263E-5
96-97	3.6705537550178127	3.325945853268914E-5	0.0	1.2305999657094983E-4	5.321513365230263E-5
98-99	4.376407602646589	3.49224314593236E-5	0.0	1.2305999657094983E-4	5.321513365230263E-5
100-101	5.166916750078617	3.658540438595806E-5	0.0	1.2472296949758428E-4	5.321513365230263E-5
102-103	6.03480236481402	3.658540438595806E-5	0.0	1.2804891535085318E-4	5.321513365230263E-5
104-105	6.998982426866192	3.658540438595806E-5	0.0	1.3137486120412211E-4	5.321513365230263E-5
106-107	8.045368229600685	3.658540438595806E-5	0.0	1.3802675291065995E-4	5.321513365230263E-5
108-109	9.17205234307065	3.824837731259251E-5	0.0	1.4135269876392885E-4	5.654107950557154E-5
110-111	10.352259240184345	3.991135023922697E-5	0.0	1.4634161754383223E-4	5.654107950557154E-5
112-113	11.594697910158553	3.991135023922697E-5	0.0	1.4634161754383223E-4	5.654107950557154E-5
114-115	12.896795733875773	3.991135023922697E-5	0.0	1.5299350925037004E-4	5.654107950557154E-5
116-117	14.244352585515472	3.991135023922697E-5	0.0	1.5964540095690787E-4	5.654107950557154E-5
118-119	15.624846278940094	3.991135023922697E-5	0.0	1.5964540095690787E-4	5.654107950557154E-5
120-121	17.015134882902593	3.991135023922697E-5	0.0	1.629713468101768E-4	5.654107950557154E-5
122-123	18.413976156626774	4.3237296092495885E-5	0.0	1.629713468101768E-4	5.654107950557154E-5
124-125	19.822519214404938	4.3237296092495885E-5	0.0	1.629713468101768E-4	5.654107950557154E-5
126-127	21.238419264410535	4.3237296092495885E-5	0.0	1.629713468101768E-4	5.8204052432206E-5
128-129	22.669470660750697	4.988918779903371E-5	0.0	1.6463431973681126E-4	5.9867025358840456E-5
130-131	24.09915675631809	4.988918779903371E-5	0.0	1.662972926634457E-4	5.9867025358840456E-5
132-133	25.51332066258828	5.654107950557154E-5	0.0	1.662972926634457E-4	5.9867025358840456E-5
134-135	26.9042661408568	5.654107950557154E-5	0.0	1.662972926634457E-4	5.9867025358840456E-5
136-137	28.265572452653913	5.654107950557154E-5	0.0	1.712862114433491E-4	5.9867025358840456E-5
138-139	29.607944154708367	5.8204052432206E-5	0.0	1.812640490031558E-4	6.152999828547491E-5
>>END_MODULE
