##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261622_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30066635
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.333279896469957	32.0	32.0	32.0	32.0	32.0
2	31.743141858076235	32.0	32.0	32.0	32.0	32.0
3	35.70762308452542	37.0	37.0	37.0	32.0	37.0
4	36.287097475324394	37.0	37.0	37.0	37.0	37.0
5	36.499878386789874	37.0	37.0	37.0	37.0	37.0
6	40.016222833050655	41.0	41.0	41.0	37.0	41.0
7	40.09595031169933	41.0	41.0	41.0	37.0	41.0
8	40.17382590369691	41.0	41.0	41.0	37.0	41.0
9	40.236190847429384	41.0	41.0	41.0	41.0	41.0
10-14	40.25799927394603	41.0	41.0	41.0	41.0	41.0
15-19	40.240119527842076	41.0	41.0	41.0	41.0	41.0
20-24	40.23302166005607	41.0	41.0	41.0	41.0	41.0
25-29	40.168745834044955	41.0	41.0	41.0	41.0	41.0
30-34	40.149442869147144	41.0	41.0	41.0	41.0	41.0
35-39	40.13509729971445	41.0	41.0	41.0	41.0	41.0
40-44	40.04956746240475	41.0	41.0	41.0	37.0	41.0
45-49	40.01858034329415	41.0	41.0	41.0	37.0	41.0
50-54	40.04804792421899	41.0	41.0	41.0	37.0	41.0
55-59	40.02093337016264	41.0	41.0	41.0	37.0	41.0
60-64	39.981669016170244	41.0	41.0	41.0	37.0	41.0
65-69	39.938696817917936	41.0	41.0	41.0	37.0	41.0
70-74	39.89555904742915	41.0	41.0	41.0	37.0	41.0
75-79	39.44031327083992	41.0	40.2	41.0	36.0	41.0
80-84	39.80410515509966	41.0	41.0	41.0	37.0	41.0
85-89	39.79022191209625	41.0	41.0	41.0	37.0	41.0
90-94	39.744537863981115	41.0	41.0	41.0	37.0	41.0
95-99	39.69339657065049	41.0	41.0	41.0	37.0	41.0
100-104	39.633821756242426	41.0	41.0	41.0	37.0	41.0
105-109	39.52573896613306	41.0	41.0	41.0	37.0	41.0
110-114	39.43455702309221	41.0	41.0	41.0	37.0	41.0
115-119	39.33513879421492	41.0	41.0	41.0	37.0	41.0
120-124	39.20141491723301	41.0	41.0	41.0	37.0	41.0
125-129	39.03824743274397	41.0	41.0	41.0	37.0	41.0
130-134	38.83404143496604	41.0	41.0	41.0	32.0	41.0
135-139	38.599983649650184	41.0	41.0	41.0	32.0	41.0
140-144	38.34246749594691	41.0	41.0	41.0	32.0	41.0
145-149	38.06153418897725	41.0	39.4	41.0	31.0	41.0
150	34.24803321023453	37.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	11.0
13	19.0
14	31.0
15	41.0
16	53.0
17	127.0
18	362.0
19	1105.0
20	2684.0
21	5597.0
22	10963.0
23	20136.0
24	36567.0
25	66320.0
26	146929.0
27	124045.0
28	120976.0
29	154982.0
30	195463.0
31	240506.0
32	294822.0
33	355240.0
34	434589.0
35	541775.0
36	698988.0
37	961620.0
38	1463718.0
39	2906290.0
40	2.1282676E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.40916987632051	9.892486956965884	10.851075197636494	40.84726796907711
2	25.01722717693438	11.489452603542352	38.84730394514659	24.646016274376674
3	24.78111368299113	15.277203451600089	23.84045304704035	36.10122981836844
4	27.197214148851	23.88808612583601	24.38820656524341	24.526493160069577
5	28.15077431516858	27.083308596609406	26.706236112304293	18.059680975917722
6	22.205897001776222	27.412781643173574	29.65881615950704	20.722505195543167
7	19.641256831400803	21.752515235364193	41.366238917225466	17.239989016009538
8	20.380497985689875	21.065005882866515	35.13138789917224	23.42310823227137
9	19.800249114877214	21.95629680258228	36.30148554285591	21.9419685396846
10-14	24.84642568312159	24.910075152351695	27.506053005587855	22.737446158938855
15-19	25.176466547011433	24.01217081867294	27.284814981373422	23.52654765294221
20-24	24.741038556822634	24.493800595253596	27.297466307791137	23.467694540132637
25-29	24.59293882017351	24.43162091989809	27.360526523567337	23.61491373636106
30-34	24.544164787094413	24.558589604725405	26.71507955202111	24.182166056159073
35-39	24.84464107956869	24.46013672781063	26.908200075785828	23.78702211683485
40-44	24.575129069591643	24.284361817546863	27.484489156983123	23.656019955878378
45-49	24.608252237881803	24.14541947657527	27.454974373095503	23.791353912447423
50-54	24.645419154344562	24.472526254342934	26.89679984197621	23.985254749336292
55-59	24.812213732495835	24.353607660455864	26.8074892021678	24.026689404880496
60-64	24.515055676823543	24.407308416877864	26.880036756345625	24.197599149952964
65-69	24.698071783522728	25.247527146537347	26.55952344738565	23.494877622554274
70-74	24.687777488715195	25.077772505767044	26.47816053846971	23.756289467048052
75-79	24.834456986847858	24.90389060479754	26.26317003522808	23.998482373126528
80-84	24.60216926264184	24.96567827226209	26.477818597907255	23.95433386718881
85-89	25.044440761756558	24.63141489116095	26.442752975684947	23.88139137139754
90-94	25.316998410949427	24.950792049099626	26.006814721066696	23.725394818884254
95-99	25.481491310746986	25.027777628476223	25.848275344401884	23.642455716374915
100-104	25.25951079014636	25.271320984320944	25.58360928647395	23.88555893905874
105-109	25.5377746465753	25.41022630561039	25.104967988789507	23.947031059024802
110-114	25.809940383159812	25.8737955178622	24.682785135164835	23.633478963813157
115-119	25.499833472933496	26.285507700412573	24.25684091186974	23.957817914784187
120-124	25.587940298173873	26.339881280703896	23.923305147545005	24.148873273577227
125-129	25.1517755116481	26.388292308781082	23.9412526188225	24.518679560748318
130-134	24.896891711050976	26.31025103096648	23.996334084193254	24.796523173789286
135-139	24.62522298634878	26.421432642984342	23.920595004638816	25.032749366028057
140-144	24.36790454816357	26.38373037026088	24.203718071391776	25.04464701018377
145-149	23.99676943973764	26.082777764501692	24.76075497574352	25.159697820017147
150	23.703379091366834	25.765914660281382	25.042604537727918	25.488101710623866
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	23.5
2	15.0
3	16.5
4	22.0
5	24.5
6	29.0
7	34.0
8	36.0
9	41.5
10	57.5
11	64.5
12	64.5
13	86.0
14	112.0
15	146.5
16	215.5
17	319.5
18	482.0
19	769.5
20	1420.5
21	2475.0
22	3747.0
23	5784.0
24	9495.5
25	15386.5
26	23184.5
27	33483.5
28	50750.0
29	76062.0
30	104391.0
31	136980.0
32	182435.0
33	242752.5
34	318124.0
35	395401.0
36	457516.0
37	576150.0
38	684037.0
39	673176.0
40	656553.0
41	736382.0
42	829830.5
43	934137.5
44	1065579.5
45	1197325.0
46	1265787.5
47	1296940.5
48	1349868.0
49	1425089.0
50	1535529.5
51	1523330.5
52	1468711.5
53	1457758.0
54	1354619.0
55	1241737.5
56	1136949.0
57	1008418.0
58	916087.0
59	807049.5
60	669796.5
61	535368.0
62	423253.5
63	323447.5
64	244168.5
65	184713.5
66	133168.0
67	99344.0
68	73936.5
69	53033.5
70	37578.5
71	24932.0
72	19005.5
73	14062.0
74	9392.0
75	6450.5
76	4632.5
77	2904.0
78	1616.5
79	988.0
80	643.0
81	479.5
82	323.5
83	174.5
84	96.0
85	45.5
86	20.5
87	10.5
88	5.5
89	5.0
90	4.5
91	4.0
92	2.0
93	2.5
94	5.0
95	4.0
96	1.5
97	1.0
98	1.5
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0225786823167942
2	0.012209547227350185
3	0.0
4	0.01954325783380814
5	6.651891706537829E-6
6	0.0
7	0.00352217665861178
8	0.0016962323851671464
9	6.152999828547491E-4
10-14	2.8004464084524256E-4
15-19	9.911318642741364E-5
20-24	1.822618327591365E-4
25-29	0.04755237824252698
30-34	0.001320400503747759
35-39	0.0046017786825828695
40-44	0.03835148163404385
45-49	0.10652405897766744
50-54	9.179610555022202E-4
55-59	0.0027658565715784292
60-64	0.0017760550856456003
65-69	0.001657651413269227
70-74	3.226167477670847E-4
75-79	7.303777093778535E-4
80-84	0.0023121975571925493
85-89	0.0014341478519295557
90-94	8.447902467303042E-4
95-99	0.002372729771722043
100-104	0.0036039949266021953
105-109	7.669631137638116E-4
110-114	0.0025536612261398725
115-119	1.7028842768736842E-4
120-124	0.001393571312519675
125-129	0.0011813759670811182
130-134	1.3835934749598683E-4
135-139	0.0018518866511001313
140-144	0.001516631309090625
145-149	0.01408005917522862
150	6.651891706537829E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	3.0066635E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.612457985823873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.71099707405108	18.817934387688993
2	13.00889972099824	6.923975945337231
3	5.130560525248125	4.0961047926567655
4	2.738746231550919	2.9153907608392915
5	1.6569210385323658	2.204737076188512
6	1.0831199113602263	1.7294689880809981
7	0.8025017778840215	1.494958139324124
8	0.6046439737322117	1.2872849877864059
9	0.4842200245691771	1.1597656553767646
>10	3.130558863906497	16.112906388640347
>50	0.3335079637514789	6.176220462080093
>100	0.26058171507510314	13.947125403261696
>500	0.03041969760088703	5.585168222932984
>1k	0.021696762134459074	11.185135085383436
>5k	0.0019880301959135306	3.5541219973404576
>10k+	6.366894091467099E-4	2.8097017070818584
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG	144735	0.4813807730728763	TruSeq Adapter, Index 7 (97% over 34bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.317080877191612E-5	0.0	0.0	3.3259458532689143E-6	3.3259458532689143E-6
2	1.0643026730460526E-4	0.0	0.0	1.662972926634457E-5	9.977837559806742E-6
3	1.662972926634457E-4	0.0	0.0	1.662972926634457E-5	2.3281620972882398E-5
4	3.059870185007401E-4	0.0	0.0	1.9955675119613484E-5	3.658540438595806E-5
5	4.0909133995207646E-4	0.0	0.0	1.9955675119613484E-5	3.991135023922697E-5
6	5.155216072566817E-4	0.0	0.0	1.9955675119613484E-5	3.991135023922697E-5
7	5.620848492024464E-4	0.0	0.0	1.9955675119613484E-5	3.991135023922697E-5
8	6.019961994416735E-4	0.0	0.0	2.3281620972882398E-5	4.3237296092495885E-5
9	8.713978135564555E-4	0.0	0.0	2.9933512679420228E-5	4.988918779903371E-5
10-11	0.0010859213210923004	0.0	0.0	2.9933512679420228E-5	4.988918779903371E-5
12-13	0.0013220634766743934	0.0	1.6629729266344572E-6	3.325945853268914E-5	5.9867025358840456E-5
14-15	0.0014567642837317843	0.0	3.3259458532689143E-6	3.325945853268914E-5	5.9867025358840456E-5
16-17	0.0015665204968896586	1.6629729266344572E-6	3.3259458532689143E-6	3.658540438595806E-5	5.9867025358840456E-5
18-19	0.001662972926634457	3.3259458532689143E-6	3.3259458532689143E-6	3.658540438595806E-5	6.319297121210937E-5
20-21	0.0017511104917460833	3.3259458532689143E-6	3.3259458532689143E-6	3.824837731259251E-5	6.818188999201273E-5
22-23	0.001844236975637613	3.3259458532689143E-6	3.3259458532689143E-6	4.4900269019130344E-5	6.98448629186472E-5
24-25	0.0019340375136758735	9.977837559806742E-6	3.3259458532689143E-6	6.319297121210937E-5	6.98448629186472E-5
26-27	0.002033815889273941	9.977837559806742E-6	3.3259458532689143E-6	6.651891706537828E-5	7.150783584528167E-5
28-29	0.0021651907504780634	9.977837559806742E-6	3.3259458532689143E-6	7.317080877191612E-5	7.982270047845395E-5
30-31	0.002331488043141509	9.977837559806742E-6	3.3259458532689143E-6	7.483378169855057E-5	8.980053803826069E-5
32-33	0.002587585873843215	1.1640810486441199E-5	3.3259458532689143E-6	7.982270047845394E-5	9.977837559806742E-5
34-35	0.00300000315964856	1.4966756339710114E-5	3.3259458532689143E-6	7.982270047845394E-5	1.16408104864412E-4
36-37	0.003547121252511297	1.9955675119613484E-5	4.988918779903372E-6	8.14856734050884E-5	1.3470080705739102E-4
38-39	0.004275503394377189	1.9955675119613484E-5	6.651891706537829E-6	8.314864633172285E-5	1.662972926634457E-4
40-41	0.005196790395732679	1.9955675119613484E-5	6.651891706537829E-6	9.312648389152959E-5	1.7627513022325244E-4
42-43	0.006435705226075349	1.9955675119613484E-5	6.651891706537829E-6	9.312648389152959E-5	1.8292702192979028E-4
44-45	0.007970629237358954	2.4944593899516856E-5	6.651891706537829E-6	9.312648389152959E-5	1.8791594070969366E-4
46-47	0.009996130261999721	2.6607566826151315E-5	6.651891706537829E-6	9.312648389152959E-5	1.895789136363281E-4
48-49	0.01245067830171218	2.6607566826151315E-5	6.651891706537829E-6	9.312648389152959E-5	1.9290485948959702E-4
50-51	0.015575404430858326	2.6607566826151315E-5	6.651891706537829E-6	9.312648389152959E-5	1.9290485948959702E-4
52-53	0.019292148921886337	2.6607566826151315E-5	6.651891706537829E-6	9.478945681816404E-5	2.1452350753584497E-4
54-55	0.02400335122304175	2.6607566826151315E-5	6.651891706537829E-6	1.0310432145133634E-4	2.1618648046247942E-4
56-57	0.029893601329181	3.658540438595806E-5	6.651891706537829E-6	1.0643026730460526E-4	2.1618648046247942E-4
58-59	0.03753995084584623	3.991135023922697E-5	6.651891706537829E-6	1.16408104864412E-4	2.1618648046247942E-4
60-61	0.04719849760373916	3.991135023922697E-5	6.651891706537829E-6	1.1973405071768091E-4	2.1951242631574835E-4
62-63	0.06004330048906371	3.991135023922697E-5	6.651891706537829E-6	1.2305999657094983E-4	2.245013450956517E-4
64-65	0.07690584596513711	3.991135023922697E-5	6.651891706537829E-6	1.2305999657094983E-4	2.3115323680218954E-4
66-67	0.09934766561006911	3.991135023922697E-5	6.651891706537829E-6	1.2305999657094983E-4	2.3946810143536182E-4
68-69	0.1293726418004542	3.991135023922697E-5	6.651891706537829E-6	1.2305999657094983E-4	2.5776080362834085E-4
70-71	0.1680583809927516	4.323729609249588E-5	6.651891706537829E-6	1.2472296949758428E-4	2.6274972240824423E-4
72-73	0.2202823827807801	4.6563241945764795E-5	6.651891706537829E-6	1.2638594242421873E-4	2.6940161411478204E-4
74-75	0.2907375567635021	4.6563241945764795E-5	6.651891706537829E-6	1.2638594242421873E-4	2.843683704544922E-4
76-77	0.3830791174336603	4.6563241945764795E-5	6.651891706537829E-6	1.3303783413075657E-4	2.8769431630776103E-4
78-79	0.4991978650088379	4.6563241945764795E-5	9.977837559806742E-6	1.3303783413075657E-4	2.976721538675678E-4
80-81	0.6457573320060592	4.6563241945764795E-5	1.1640810486441199E-5	1.3470080705739102E-4	2.993351267942023E-4
82-83	0.8311522057589751	4.6563241945764795E-5	1.3303783413075657E-5	1.396897258372944E-4	3.059870185007401E-4
84-85	1.0614839339354072	4.6563241945764795E-5	1.3303783413075657E-5	1.5798242803027342E-4	3.0764999142737456E-4
86-87	1.3494592926677695	4.988918779903371E-5	1.3303783413075657E-5	1.629713468101768E-4	3.1263891020727794E-4
88-89	1.6965899243463727	4.988918779903371E-5	1.3303783413075657E-5	1.662972926634457E-4	3.1929080191381575E-4
90-91	2.1052472283645978	4.988918779903371E-5	1.3303783413075657E-5	1.696232385167146E-4	3.2261674776708465E-4
92-93	2.588879666780137	5.487810657893708E-5	1.3303783413075657E-5	1.74612157296618E-4	3.276056665469881E-4
94-95	3.1541906834602544	5.654107950557154E-5	1.3303783413075657E-5	1.7627513022325244E-4	3.325945853268914E-4
96-97	3.798185264164081	5.654107950557154E-5	1.662972926634457E-5	1.8126404900315583E-4	3.392464770334292E-4
98-99	4.529224836766735	5.8204052432206E-5	1.662972926634457E-5	1.9456783241623147E-4	3.425724228866982E-4
100-101	5.3503925530741965	5.9867025358840456E-5	1.662972926634457E-5	2.0787161582930713E-4	3.5088728751987046E-4
102-103	6.2492876239725526	5.9867025358840456E-5	1.662972926634457E-5	2.0953458875594159E-4	3.558762062997738E-4
104-105	7.247731580205101	5.9867025358840456E-5	1.662972926634457E-5	2.1286053460921052E-4	3.5753917922640827E-4
106-107	8.330154006259763	6.319297121210937E-5	1.662972926634457E-5	2.1951242631574835E-4	3.5920215215304275E-4
108-109	9.492821527916243	6.319297121210937E-5	1.662972926634457E-5	2.2283837216901725E-4	3.6086512507967723E-4
110-111	10.713373811203017	6.319297121210937E-5	1.662972926634457E-5	2.278272909489206E-4	3.6252809800631165E-4
112-113	11.993598552016213	6.319297121210937E-5	1.662972926634457E-5	2.294902638755551E-4	3.6751701678621504E-4
114-115	13.335848857047022	6.319297121210937E-5	1.662972926634457E-5	2.3447918265545847E-4	3.6917998971284946E-4
116-117	14.729069282279177	6.319297121210937E-5	1.662972926634457E-5	2.3614215558209292E-4	3.6917998971284946E-4
118-119	16.164645627952712	6.485594413874383E-5	1.662972926634457E-5	2.3614215558209292E-4	3.6917998971284946E-4
120-121	17.612604470037965	6.818188999201273E-5	1.662972926634457E-5	2.3614215558209292E-4	3.6917998971284946E-4
122-123	19.071031726696386	7.150783584528167E-5	1.662972926634457E-5	2.3946810143536182E-4	3.7416890849275284E-4
124-125	20.543725628092403	7.317080877191612E-5	1.662972926634457E-5	2.3946810143536182E-4	3.957875565390008E-4
126-127	22.028527635367244	7.317080877191612E-5	1.662972926634457E-5	2.4279404728863073E-4	4.0909133995207646E-4
128-129	23.526611807407114	7.317080877191612E-5	1.662972926634457E-5	2.4279404728863073E-4	4.0909133995207646E-4
130-131	25.02478245403917	7.317080877191612E-5	1.662972926634457E-5	2.4611999314189966E-4	4.0909133995207646E-4
132-133	26.511543443421587	7.317080877191612E-5	1.662972926634457E-5	2.4611999314189966E-4	4.0909133995207646E-4
134-135	27.97852336984169	7.317080877191612E-5	1.662972926634457E-5	2.4611999314189966E-4	4.1075431287871094E-4
136-137	29.415852488979894	7.317080877191612E-5	1.829270219297903E-5	2.4944593899516856E-4	4.2904701507168994E-4
138-139	30.832638903555385	7.815972755181947E-5	2.161864804624794E-5	2.544348577750719E-4	4.340359338515933E-4
>>END_MODULE
