FastQCFastQC Report
Mon 24 Jun 2019
SRR8261621_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8261621_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30065066
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC884370.2941520234813388Illumina Single End PCR Primer 1 (96% over 33bp)
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT527910.17558917050107256No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC314420.10457984692267099No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)