FastQCFastQC Report
Mon 24 Jun 2019
SRR8261621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8261621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30065066
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG852060.2834053316230871TruSeq Adapter, Index 7 (97% over 34bp)
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT329710.10966548352163938No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCATC304960.10143333794776968No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC301800.10038228420985339No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)