##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261620_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31813557
Sequences flagged as poor quality	0
Sequence length	150
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.78793650769702	32.0	32.0	32.0	32.0	32.0
2	31.3548605709195	32.0	32.0	32.0	32.0	32.0
3	35.06283010101637	37.0	37.0	37.0	32.0	37.0
4	35.65674891367853	37.0	37.0	37.0	32.0	37.0
5	35.869228832223946	37.0	37.0	37.0	37.0	37.0
6	39.21852976075577	41.0	41.0	41.0	37.0	41.0
7	39.35895445454276	41.0	41.0	41.0	37.0	41.0
8	39.39330883371514	41.0	41.0	41.0	37.0	41.0
9	39.42812050221231	41.0	41.0	41.0	37.0	41.0
10-14	39.43432703862696	41.0	41.0	41.0	37.0	41.0
15-19	39.39305183007358	41.0	41.0	41.0	37.0	41.0
20-24	39.35235653781185	41.0	41.0	41.0	37.0	41.0
25-29	39.31568957850265	41.0	41.0	41.0	37.0	41.0
30-34	39.28792718777093	41.0	41.0	41.0	37.0	41.0
35-39	39.20831099144305	41.0	41.0	41.0	37.0	41.0
40-44	39.15750955481023	41.0	41.0	41.0	37.0	41.0
45-49	39.083125995625075	41.0	41.0	41.0	37.0	41.0
50-54	38.99902254249658	41.0	41.0	41.0	37.0	41.0
55-59	38.941984500507125	41.0	41.0	41.0	35.0	41.0
60-64	38.88751712359608	41.0	41.0	41.0	34.0	41.0
65-69	38.829476609610175	41.0	41.0	41.0	32.0	41.0
70-74	38.77034959655721	41.0	41.0	41.0	32.0	41.0
75-79	38.301703981104666	41.0	39.4	41.0	31.0	41.0
80-84	38.788291859347886	41.0	41.0	41.0	32.0	41.0
85-89	38.7600664961796	41.0	41.0	41.0	32.0	41.0
90-94	38.59971443620718	41.0	41.0	41.0	32.0	41.0
95-99	38.44440161783858	41.0	41.0	41.0	32.0	41.0
100-104	38.250261408996174	41.0	41.0	41.0	32.0	41.0
105-109	38.055238959918874	41.0	37.8	41.0	32.0	41.0
110-114	37.78189336074554	41.0	37.0	41.0	31.0	41.0
115-119	37.45901357713632	41.0	37.0	41.0	27.0	41.0
120-124	37.17173845100062	41.0	37.0	41.0	27.0	41.0
125-129	36.775268128615735	41.0	37.0	41.0	26.0	41.0
130-134	36.20119483652834	41.0	37.0	41.0	22.0	41.0
135-139	35.91043799346298	41.0	36.0	41.0	22.0	41.0
140-144	35.508988906836166	41.0	32.0	41.0	22.0	41.0
145-149	35.17497725890883	41.0	32.0	41.0	22.0	41.0
150	29.393042846482082	32.0	22.0	37.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	17.0
12	127.0
13	560.0
14	5644.0
15	29198.0
16	69036.0
17	91719.0
18	97327.0
19	99530.0
20	106589.0
21	116312.0
22	128516.0
23	145147.0
24	166702.0
25	197322.0
26	252565.0
27	285958.0
28	262864.0
29	292257.0
30	335004.0
31	390180.0
32	458325.0
33	547900.0
34	668153.0
35	839324.0
36	1102340.0
37	1541057.0
38	2441053.0
39	5253417.0
40	1.5889414E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.64979062315657	20.1610423679636	12.311985740033435	38.87718126884639
2	25.068000822655755	26.028450157177925	30.162741729791538	18.740807290374782
3	21.061233873590645	26.507906835923116	25.820030483361094	26.61082880712514
4	24.034890836551998	33.26245333883877	21.132154517549775	21.570501307059452
5	26.487255103225333	34.49995233164277	19.959604014100027	19.053188551031877
6	21.362185309866483	34.058332427273065	21.815840963649556	22.7636412992109
7	21.48425891820914	20.3687547043699	32.9031449724529	25.243841404968066
8	21.13353396836501	23.215268849171704	26.94009845749835	28.711098724964927
9	21.308151741724448	24.0783229614972	28.39809141744194	26.21543387933641
10-14	24.77041505757433	26.497930644528196	23.34898967612806	25.38266462176942
15-19	24.609693282646766	26.471773024311616	24.101509303093646	24.81702438994797
20-24	24.539598648438997	26.693605146279104	24.215440921963594	24.551355283318305
25-29	24.222581598153635	26.676971418016716	24.32912175400914	24.77132522982051
30-34	24.08876740900131	26.554638926241548	24.462046731454247	24.89454693330289
35-39	23.852981494753635	26.834141484586123	24.38702877460537	24.925848246054876
40-44	23.97258612731332	26.47137621877123	24.728887254679893	24.827150399235556
45-49	23.969846021404983	26.49394092822293	24.640642031102356	24.895571019269727
50-54	24.216856396136727	26.554766765904876	24.408284332425445	24.820092505532955
55-59	24.234697806712106	26.40267561662085	24.42543374512157	24.937192831545474
60-64	24.02332098576398	26.394570310043296	24.825973555177345	24.756135149015382
65-69	24.21223798681951	26.356539633590792	24.860282153864063	24.570940225725632
70-74	24.20347639136488	26.83962370786596	24.512516939666533	24.44438296110262
75-79	24.127586687541967	26.909097536342447	24.467998926042345	24.49531685007324
80-84	24.117896265088373	27.134968113857237	24.35520047895885	24.391935142095537
85-89	24.36618453628858	27.05027801460845	23.962648293092546	24.620889156010428
90-94	24.512363764339206	27.15775866202436	23.99520653180672	24.33467104182971
95-99	24.703814743911305	27.271935099051593	23.870140391840934	24.154109765196168
100-104	25.416903644138873	27.594966894276734	23.31597619579274	23.672153265791653
105-109	25.775038433698438	27.948079721405577	22.906974629380368	23.369907215515614
110-114	26.44656760010024	27.99546077045532	22.62591905702838	22.932052572416055
115-119	27.16362132464598	27.89269688363834	22.559285403304095	22.384396388411584
120-124	27.894963107907543	27.90478659865135	22.428569592874965	21.771680700566147
125-129	28.657797135346986	27.859257799634925	22.351069554399295	21.131875510618798
130-134	29.467816815581727	27.68399132660765	22.289797181493253	20.55839467631737
135-139	29.763324502467846	27.575585399902565	22.632290241085702	20.028799856543884
140-144	30.10956241076721	27.435054181461066	22.707794038874685	19.74758936889704
145-149	30.542279977100517	26.98319011433597	23.040277303415476	19.43425260514804
150	30.176657188416367	27.018184114517034	23.380144747816	19.425013949250598
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	73.0
1	77.0
2	87.5
3	92.0
4	115.5
5	164.5
6	193.0
7	217.0
8	257.0
9	288.0
10	315.5
11	366.5
12	432.5
13	515.0
14	609.0
15	708.0
16	879.5
17	1160.0
18	1477.5
19	1895.5
20	2824.0
21	4241.0
22	6381.5
23	9381.5
24	13940.5
25	20427.0
26	30500.5
27	44145.0
28	61055.0
29	87711.0
30	117395.5
31	151725.5
32	201137.5
33	274198.0
34	359964.5
35	421565.0
36	483075.0
37	591456.5
38	660968.5
39	676273.0
40	712213.5
41	790977.5
42	878234.5
43	964578.5
44	1048530.5
45	1134520.5
46	1243378.0
47	1315582.0
48	1386277.0
49	1514870.5
50	1621445.0
51	1589887.0
52	1517340.0
53	1488874.5
54	1414041.0
55	1344964.5
56	1274923.0
57	1135707.0
58	998391.5
59	855060.0
60	711354.5
61	586781.5
62	474611.0
63	383420.5
64	296521.0
65	224405.0
66	169188.5
67	128613.0
68	100749.5
69	78044.5
70	57414.0
71	41008.0
72	31593.5
73	22949.5
74	15516.5
75	10732.5
76	7144.5
77	4813.0
78	3133.5
79	2103.0
80	1444.5
81	1002.0
82	680.0
83	469.5
84	329.5
85	228.5
86	184.0
87	149.5
88	125.0
89	109.0
90	84.0
91	73.0
92	66.5
93	56.0
94	51.0
95	49.5
96	42.0
97	37.0
98	36.5
99	33.0
100	89.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.7943513829654447
2	0.01377714538490619
3	4.2749070781365316E-4
4	5.060735585146923E-4
5	0.0
6	0.0
7	1.6030901543011994E-4
8	7.418221106178099E-4
9	0.0
10-14	3.9228559069958763E-4
15-19	0.0
20-24	9.995738609172184E-5
25-29	3.8977093947715435E-5
30-34	3.6210977603038855E-4
35-39	2.564944246881919E-4
40-44	0.0012843581118577845
45-49	2.2066064476851803E-4
50-54	0.001700532889170488
55-59	4.7212576701184336E-4
60-64	3.652530900584301E-4
65-69	8.248056009581072E-4
70-74	1.9425680693296884E-4
75-79	3.834843114210712E-5
80-84	0.0017369953318957698
85-89	4.928716395969178E-4
90-94	3.105594259705069E-4
95-99	1.7539692276471945E-4
100-104	1.722536087366779E-4
105-109	6.261481543858803E-4
110-114	1.6470965506937813E-4
115-119	2.9547151863590734E-5
120-124	5.846564092157315E-5
125-129	6.167182123017555E-4
130-134	9.492808364685533E-5
135-139	2.0494407462831019E-4
140-144	0.0
145-149	2.722109948283997E-4
150	6.286628056083134E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	3.1813557E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.621029234479742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.51664296937957	23.24671916555561
2	11.96623823182109	7.567695379103277
3	4.656675211427687	4.417465889880961
4	2.4502119132330713	3.099128901560539
5	1.4635909578506026	2.3140126232757052
6	1.0057063710990728	1.908088233709579
7	0.6862901803692458	1.519084129975461
8	0.5165478439476875	1.3067019579581844
9	0.4093191674105402	1.164878402302944
>10	2.782894152309509	17.04779747760822
>50	0.29204254227880094	6.448571133935207
>100	0.2151271055391952	13.504917919357991
>500	0.023150754367176812	5.050026603353428
>1k	0.014776031797632906	8.67693771769597
>5k	5.692262409098252E-4	1.1926219878717237
>10k+	2.1734092832501742E-4	1.5353524768551863
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC	153943	0.48389119141880305	Illumina Single End PCR Primer 1 (96% over 31bp)
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT	43647	0.1371962273819303	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.972296653278978E-5	3.1433140280415674E-6	0.0	3.1433140280415674E-6	0.0
2	7.229622264495605E-5	3.1433140280415674E-6	0.0	3.1433140280415674E-6	0.0
3	9.744273486928858E-5	3.1433140280415674E-6	0.0	6.286628056083135E-6	0.0
4	2.0745872585074343E-4	3.1433140280415674E-6	0.0	6.286628056083135E-6	0.0
5	2.7975494849569947E-4	3.1433140280415674E-6	0.0	6.286628056083135E-6	0.0
6	4.2120407975757E-4	3.1433140280415674E-6	0.0	9.429942084124703E-6	3.1433140280415674E-6
7	4.43207277953861E-4	3.1433140280415674E-6	0.0	9.429942084124703E-6	3.1433140280415674E-6
8	4.683537901781935E-4	3.1433140280415674E-6	0.0	1.8859884168249405E-5	3.1433140280415674E-6
9	6.946724001971864E-4	3.1433140280415674E-6	0.0	1.8859884168249405E-5	3.1433140280415674E-6
10-11	9.068460970899921E-4	3.1433140280415674E-6	0.0	1.8859884168249405E-5	7.858285070103919E-6
12-13	0.0011268780790529018	3.1433140280415674E-6	0.0	1.8859884168249405E-5	1.8859884168249405E-5
14-15	0.0012698988673287932	3.1433140280415674E-6	0.0	1.8859884168249405E-5	1.8859884168249405E-5
16-17	0.0014443527958851003	3.1433140280415674E-6	0.0	1.8859884168249405E-5	1.8859884168249405E-5
18-19	0.0016093767823572823	3.1433140280415674E-6	0.0	2.043154118227019E-5	1.8859884168249405E-5
20-21	0.0017162494593106958	3.1433140280415674E-6	0.0	2.3574855210311756E-5	1.8859884168249405E-5
22-23	0.0018294087643201922	3.1433140280415674E-6	0.0	2.8289826252374106E-5	1.8859884168249405E-5
24-25	0.0019519980114138134	3.1433140280415674E-6	0.0	2.8289826252374106E-5	1.8859884168249405E-5
26-27	0.0021107353698299122	3.1433140280415674E-6	0.0	3.143314028041567E-5	1.8859884168249405E-5
28-29	0.0023244807237367392	3.1433140280415674E-6	0.0	3.4576454308457244E-5	1.8859884168249405E-5
30-31	0.002612093957302542	3.1433140280415674E-6	0.0	3.771976833649881E-5	2.2003198196290972E-5
32-33	0.0030600162062984658	3.1433140280415674E-6	0.0	4.086308236454038E-5	2.986148326639489E-5
34-35	0.0037751201476779223	3.1433140280415674E-6	0.0	4.086308236454038E-5	3.143314028041567E-5
36-37	0.004608098365108938	3.1433140280415674E-6	0.0	4.086308236454038E-5	3.143314028041567E-5
38-39	0.005711401588951528	6.286628056083135E-6	0.0	4.4006396392581944E-5	3.143314028041567E-5
40-41	0.007017448567602799	6.286628056083135E-6	0.0	4.5578053406602725E-5	3.4576454308457244E-5
42-43	0.00882328247671268	6.286628056083135E-6	0.0	4.714971042062351E-5	3.4576454308457244E-5
44-45	0.01102831726738384	6.286628056083135E-6	0.0	4.87213674346443E-5	3.771976833649881E-5
46-47	0.013634124596630298	6.286628056083135E-6	0.0	5.815130951876899E-5	3.771976833649881E-5
48-49	0.01695032089621415	6.286628056083135E-6	0.0	5.972296653278978E-5	3.771976833649881E-5
50-51	0.020931328112728798	6.286628056083135E-6	0.0	6.286628056083134E-5	3.929142535051959E-5
52-53	0.026075361519618822	6.286628056083135E-6	0.0	6.286628056083134E-5	4.086308236454038E-5
54-55	0.03245157402550114	9.429942084124703E-6	1.5716570140207837E-6	6.915290861691449E-5	4.2434739378561164E-5
56-57	0.03994680632536626	1.1001599098145486E-5	3.1433140280415674E-6	7.543953667299762E-5	4.4006396392581944E-5
58-59	0.04918186293975238	1.257325611216627E-5	3.1433140280415674E-6	7.543953667299762E-5	4.4006396392581944E-5
60-61	0.06078540667426783	1.257325611216627E-5	3.1433140280415674E-6	7.70111936870184E-5	4.4006396392581944E-5
62-63	0.07595818348762448	1.4144913126187051E-5	3.1433140280415674E-6	8.329782174310154E-5	4.4006396392581944E-5
64-65	0.09542944223432796	1.5716570140207835E-5	3.1433140280415674E-6	8.958444979918466E-5	4.4006396392581944E-5
66-67	0.121520520324087	1.5716570140207835E-5	3.1433140280415674E-6	9.115610681320545E-5	4.714971042062351E-5
68-69	0.15593352230308608	1.5716570140207835E-5	3.1433140280415674E-6	9.115610681320545E-5	4.714971042062351E-5
70-71	0.2002039570740235	1.5716570140207835E-5	3.1433140280415674E-6	9.587107785526781E-5	4.714971042062351E-5
72-73	0.25774232035732436	1.5716570140207835E-5	3.1433140280415674E-6	9.901439188330937E-5	4.87213674346443E-5
74-75	0.3325531942247137	1.5716570140207835E-5	3.1433140280415674E-6	1.0058604889733016E-4	5.186468146268586E-5
76-77	0.42847456510443016	1.5716570140207835E-5	3.1433140280415674E-6	1.0844433396743408E-4	5.3436338476706645E-5
78-79	0.5511062469374298	1.7288227154228622E-5	3.1433140280415674E-6	1.1315930500949642E-4	5.3436338476706645E-5
80-81	0.704404100428003	1.8859884168249405E-5	3.1433140280415674E-6	1.147309620235172E-4	5.500799549072743E-5
82-83	0.8970971086320212	1.8859884168249405E-5	3.1433140280415674E-6	1.1630261903753798E-4	5.657965250474821E-5
84-85	1.1354829012046657	1.8859884168249405E-5	3.1433140280415674E-6	1.1630261903753798E-4	5.657965250474821E-5
86-87	1.4330274354420665	1.8859884168249405E-5	3.1433140280415674E-6	1.2101759007960035E-4	5.815130951876899E-5
88-89	1.7894258098835034	1.8859884168249405E-5	3.1433140280415674E-6	1.335908461917666E-4	6.129462354681055E-5
90-91	2.2112585524466817	2.2003198196290972E-5	3.1433140280415674E-6	1.367341602198082E-4	6.443793757485213E-5
92-93	2.709632563249686	2.2003198196290972E-5	3.1433140280415674E-6	1.3830581723382897E-4	6.600959458887291E-5
94-95	3.2872636656127447	2.2003198196290972E-5	3.1433140280415674E-6	1.3987747424784976E-4	6.600959458887291E-5
96-97	3.9476142199377455	2.2003198196290972E-5	3.1433140280415674E-6	1.4144913126187052E-4	6.600959458887291E-5
98-99	4.697739394560627	2.3574855210311756E-5	3.1433140280415674E-6	1.445924452899121E-4	6.600959458887291E-5
100-101	5.538105971614554	2.514651222433254E-5	3.1433140280415674E-6	1.4616410230393288E-4	6.600959458887291E-5
102-103	6.462623780170196	2.986148326639489E-5	3.1433140280415674E-6	1.4930741633197446E-4	6.600959458887291E-5
104-105	7.490591196702714	3.4576454308457244E-5	3.1433140280415674E-6	1.5087907334599524E-4	6.600959458887291E-5
106-107	8.602845007240152	3.4576454308457244E-5	3.1433140280415674E-6	1.52450730360016E-4	6.600959458887291E-5
108-109	9.800087742467777	3.4576454308457244E-5	3.1433140280415674E-6	1.5716570140207836E-4	6.75812516028937E-5
110-111	11.05520203226568	3.771976833649881E-5	3.1433140280415674E-6	1.650239864721823E-4	6.915290861691449E-5
112-113	12.38241923089581	4.2434739378561164E-5	3.1433140280415674E-6	1.728822715422862E-4	6.915290861691449E-5
114-115	13.764105346660859	4.4006396392581944E-5	3.1433140280415674E-6	1.728822715422862E-4	6.915290861691449E-5
116-117	15.199290667183176	4.4006396392581944E-5	3.1433140280415674E-6	1.7602558557032775E-4	6.915290861691449E-5
118-119	16.669883848574365	4.5578053406602725E-5	3.1433140280415674E-6	1.7916889959836932E-4	6.915290861691449E-5
120-121	18.153238884919407	4.87213674346443E-5	3.1433140280415674E-6	1.8388387064043169E-4	6.915290861691449E-5
122-123	19.657212489631384	5.029302444866508E-5	3.1433140280415674E-6	1.917421557105356E-4	7.229622264495605E-5
124-125	21.17252874301355	5.029302444866508E-5	3.1433140280415674E-6	1.9331381272455638E-4	7.229622264495605E-5
126-127	22.69096945053959	5.029302444866508E-5	3.1433140280415674E-6	1.9802878376661874E-4	7.229622264495605E-5
128-129	24.22824646737867	5.029302444866508E-5	3.1433140280415674E-6	2.0117209779466031E-4	7.543953667299762E-5
130-131	25.760929530765768	5.029302444866508E-5	3.1433140280415674E-6	2.0903038286476422E-4	7.858285070103918E-5
132-133	27.274488671606257	5.029302444866508E-5	3.1433140280415674E-6	2.1374535390682658E-4	7.858285070103918E-5
134-135	28.765026180505373	5.029302444866508E-5	3.1433140280415674E-6	2.1374535390682658E-4	7.858285070103918E-5
136-137	30.220808066196433	5.029302444866508E-5	3.1433140280415674E-6	2.1374535390682658E-4	7.858285070103918E-5
138-139	31.654847648755528	5.186468146268586E-5	4.714971042062351E-6	2.1688866793486816E-4	8.015450771505997E-5
>>END_MODULE
