FastQCFastQC Report
Mon 24 Jun 2019
SRR8261619_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8261619_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31194702
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC1620170.5193734500172498Illumina Single End PCR Primer 1 (96% over 32bp)
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT655310.21007092806977287No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC387700.12428392487929521No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT380670.12203033707454554No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA329750.10570705243473716No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG320310.1026808975447177No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)