##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261619_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31194702
Sequences flagged as poor quality	0
Sequence length	150
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.7527792059049	32.0	32.0	32.0	32.0	32.0
2	31.317199760395212	32.0	32.0	32.0	32.0	32.0
3	34.97024587700822	37.0	32.0	37.0	32.0	37.0
4	35.576196528500255	37.0	37.0	37.0	32.0	37.0
5	35.80719485635734	37.0	37.0	37.0	37.0	37.0
6	39.12621431677725	41.0	41.0	41.0	37.0	41.0
7	39.2743562672918	41.0	41.0	41.0	37.0	41.0
8	39.306539488660604	41.0	41.0	41.0	37.0	41.0
9	39.344549148121374	41.0	41.0	41.0	37.0	41.0
10-14	39.351258152746574	41.0	41.0	41.0	37.0	41.0
15-19	39.30915215025935	41.0	41.0	41.0	37.0	41.0
20-24	39.26871675837775	41.0	41.0	41.0	37.0	41.0
25-29	39.23335275970901	41.0	41.0	41.0	37.0	41.0
30-34	39.20774084650656	41.0	41.0	41.0	37.0	41.0
35-39	39.10900415718028	41.0	41.0	41.0	37.0	41.0
40-44	39.05036680908187	41.0	41.0	41.0	37.0	41.0
45-49	38.97879927174813	41.0	41.0	41.0	37.0	41.0
50-54	38.893664661390254	41.0	41.0	41.0	33.0	41.0
55-59	38.83567425006977	41.0	41.0	41.0	32.0	41.0
60-64	38.77629022389764	41.0	41.0	41.0	32.0	41.0
65-69	38.716471822683225	41.0	41.0	41.0	32.0	41.0
70-74	38.6608001576678	41.0	41.0	41.0	32.0	41.0
75-79	38.17849759872686	41.0	39.4	41.0	31.0	41.0
80-84	38.67147737458752	41.0	41.0	41.0	32.0	41.0
85-89	38.64075110574866	41.0	41.0	41.0	32.0	41.0
90-94	38.4782335090106	41.0	41.0	41.0	32.0	41.0
95-99	38.32022097213816	41.0	41.0	41.0	32.0	41.0
100-104	38.118894016041565	41.0	39.4	41.0	32.0	41.0
105-109	37.91389507102841	41.0	37.0	41.0	32.0	41.0
110-114	37.62818854945305	41.0	37.0	41.0	29.0	41.0
115-119	37.29147830295029	41.0	37.0	41.0	27.0	41.0
120-124	36.990521185296146	41.0	37.0	41.0	27.0	41.0
125-129	36.57185496434619	41.0	37.0	41.0	24.0	41.0
130-134	35.96554666879011	41.0	37.0	41.0	22.0	41.0
135-139	35.6604178876272	41.0	33.0	41.0	22.0	41.0
140-144	35.23253898690874	41.0	32.0	41.0	22.0	41.0
145-149	34.87047391573094	41.0	32.0	41.0	22.0	41.0
150	29.126077402502514	32.0	22.0	37.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	16.0
12	122.0
13	696.0
14	6763.0
15	33431.0
16	76018.0
17	100390.0
18	105560.0
19	106720.0
20	111853.0
21	120723.0
22	134346.0
23	150772.0
24	173510.0
25	205049.0
26	264352.0
27	292559.0
28	267890.0
29	299748.0
30	342615.0
31	398343.0
32	467030.0
33	556965.0
34	681657.0
35	851916.0
36	1119312.0
37	1563978.0
38	2479801.0
39	5339494.0
40	1.4943073E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.22313372928077	21.366686136013545	12.88263019289341	35.527549941812275
2	25.98936426462293	26.656358830181322	28.492699493939007	18.86157741125674
3	21.316801425274882	27.171289953781663	25.755231427649655	25.756677193293804
4	24.272095295043485	33.486830128229684	21.16201565971207	21.07905891701476
5	26.129850511154107	34.96509118759974	19.856496785896528	19.048561515349625
6	21.24998661631709	34.165381672823806	21.742329835367556	22.84230187549155
7	21.407407305300588	20.780536731361867	32.61131392553149	25.20074203780605
8	21.42899297706619	23.57748620726985	26.545440860064073	28.448079955599887
9	21.220430315378554	24.68749020266326	27.97984414148274	26.112235340475443
10-14	24.660335866038267	26.688799785090872	23.345596574789596	25.30526777408127
15-19	24.42851353412512	26.52449701234524	24.239304481895676	24.807684971633964
20-24	24.46702608779546	26.72537984266727	24.2254624529104	24.58213161662687
25-29	24.054642097688927	26.85108229419648	24.336779234100504	24.75749637401409
30-34	23.990518051018793	26.797743714573873	24.26049939913638	24.951238835270953
35-39	23.614445828949272	26.85215921129186	24.490267179540464	25.043127780218406
40-44	23.78533693692416	26.587022524755056	24.78360672939254	24.84403380892824
45-49	23.8050849493506	26.599150416414385	24.723572716700918	24.8721919175341
50-54	24.061212985939076	26.682223471359478	24.38493712292652	24.871626419774923
55-59	24.032406475442787	26.556916874302257	24.479080573919124	24.931596076335833
60-64	23.87783205830173	26.399414241705347	24.991877753916505	24.730875946076416
65-69	24.104813115585518	26.312770819032178	25.025801386857083	24.55661467852522
70-74	24.002585137050694	26.832064865108013	24.707770558629825	24.45757943921147
75-79	23.93949923704317	26.89574426127608	24.615725916101177	24.54903058557958
80-84	23.861095662700528	27.08995324494778	24.54368395692274	24.505267135428955
85-89	24.089383404020502	27.036928034083513	24.16204934059344	24.71163922130254
90-94	24.236867432526736	27.088750110708286	24.221843034003726	24.452539422761248
95-99	24.348728454528867	27.096302516355593	24.253865402805634	24.30110362630991
100-104	25.054153525735927	27.54585748641062	23.63873494081266	23.761254047040794
105-109	25.259408030477264	27.914811636405982	23.155625943166925	23.670154389949825
110-114	25.90158326697206	27.93827157019031	22.900592449655456	23.259552713182178
115-119	26.51021128550097	27.75142952688418	22.888158464039172	22.85020072357568
120-124	27.257667012452124	27.81020866378498	22.674308567999415	22.25781575576348
125-129	28.040232781014225	27.662734217389094	22.52996640164433	21.767066599952347
130-134	28.803348418282624	27.506710842163447	22.36206641589946	21.32787432365447
135-139	29.003219406240067	27.261499992200584	22.76732579861443	20.967954802944924
140-144	29.43608501212802	27.054611388818522	22.76236586584478	20.74693773320867
145-149	29.95438174155624	26.438356276801983	23.094719541143363	20.512542440498414
150	29.542940349349944	26.43968373967344	23.363386854316605	20.653989056660013
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	84.0
1	75.0
2	80.0
3	91.0
4	110.5
5	140.5
6	170.0
7	215.0
8	247.5
9	292.0
10	337.0
11	371.5
12	455.5
13	535.0
14	631.0
15	747.0
16	881.0
17	1097.5
18	1356.5
19	1728.0
20	2528.5
21	3652.0
22	5399.5
23	7880.0
24	11538.5
25	16837.5
26	25182.0
27	36644.5
28	51592.5
29	77806.0
30	107094.5
31	142471.5
32	195041.5
33	283263.0
34	398305.0
35	465625.0
36	525879.5
37	662597.0
38	736605.5
39	719216.5
40	733153.5
41	801885.0
42	869611.0
43	935746.5
44	1017499.5
45	1115721.5
46	1222586.5
47	1287613.5
48	1377692.5
49	1522653.0
50	1587634.5
51	1487649.0
52	1377000.0
53	1342637.5
54	1297479.0
55	1271987.0
56	1229249.0
57	1097115.5
58	960466.5
59	823979.5
60	688927.5
61	571379.5
62	465536.5
63	378109.5
64	294285.5
65	224324.0
66	170343.5
67	131006.0
68	103825.0
69	81176.0
70	61619.5
71	46913.0
72	37814.5
73	28568.0
74	19977.5
75	14242.5
76	9918.0
77	6891.5
78	4700.0
79	3281.5
80	2195.5
81	1511.5
82	1132.0
83	716.0
84	477.5
85	346.0
86	250.0
87	195.0
88	143.5
89	115.0
90	93.0
91	83.0
92	74.0
93	59.0
94	54.5
95	52.0
96	44.5
97	34.5
98	31.0
99	28.0
100	58.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.7849088604853478
2	0.013441385014673325
3	5.064962633719021E-4
4	4.1994310444126053E-4
5	0.0
6	0.0
7	2.083687159441369E-4
8	6.058721125144904E-4
9	0.0
10-14	3.9109205146438007E-4
15-19	0.0
20-24	7.437160322929194E-5
25-29	4.4238281231216767E-5
30-34	3.2890200393643766E-4
35-39	2.4170771049519886E-4
40-44	0.001312402343192764
45-49	2.5196586266475635E-4
50-54	0.0017624787696320996
55-59	4.6931046175725604E-4
60-64	4.128906248246898E-4
65-69	8.168053665010167E-4
70-74	2.2311480968787583E-4
75-79	4.6161684763008795E-5
80-84	0.0017772248633758387
85-89	5.39835257922964E-4
90-94	3.487771737649553E-4
95-99	1.5643682058575202E-4
100-104	1.833644700308405E-4
105-109	6.327997619595788E-4
110-114	1.647715692235175E-4
115-119	3.398012906165925E-5
120-124	7.052479616570788E-5
125-129	6.38569972554955E-4
130-134	1.0578719424856184E-4
135-139	2.1734459909249977E-4
140-144	0.0
145-149	2.7953464662044215E-4
150	6.731912361272116E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	3.1194702E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.366124034872435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4807773236485	23.048071757124003
2	12.17650012318207	7.638592263487364
3	4.67028888900511	4.394665817136626
4	2.4137995669493897	3.028461464490237
5	1.479702490178519	2.320626592082452
6	0.9678373912031942	1.8214384594840045
7	0.7272112766532238	1.5966859372144069
8	0.5090092062448363	1.2772516718373998
9	0.39779225908091553	1.1229481204599696
>10	2.6529749753143275	15.992104907900037
>50	0.27572423245583266	6.011602061244686
>100	0.2097019419455647	13.169133854430251
>500	0.02357199425329485	5.082378893628825
>1k	0.013589039671317735	8.00825529107633
>5k	0.0010411918947806332	2.2320448702679494
>10k+	4.780983190063945E-4	3.255738038135457
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC	162017	0.5193734500172498	Illumina Single End PCR Primer 1 (96% over 32bp)
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT	65531	0.21007092806977287	No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC	38770	0.12428392487929521	No Hit
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT	38067	0.12203033707454554	No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA	32975	0.10570705243473716	No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG	32031	0.1026808975447177	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1673743188827386E-5	0.0	0.0	0.0	0.0
2	5.7702105953760994E-5	0.0	0.0	3.205672552986722E-6	0.0
3	8.014181382466805E-5	0.0	0.0	6.411345105973444E-6	0.0
4	1.4746093743738922E-4	0.0	0.0	9.617017658960165E-6	0.0
5	2.051630433911502E-4	0.0	0.0	1.6028362764933608E-5	0.0
6	2.7248216700387134E-4	0.0	0.0	1.6028362764933608E-5	3.205672552986722E-6
7	3.0133321998075186E-4	0.0	0.0	1.6028362764933608E-5	3.205672552986722E-6
8	3.269786004046456E-4	0.0	0.0	1.923403531792033E-5	3.205672552986722E-6
9	4.135317593352871E-4	0.0	0.0	1.923403531792033E-5	3.205672552986722E-6
10-11	4.920707368834619E-4	0.0	0.0	2.083687159441369E-5	3.205672552986722E-6
12-13	6.04269276237997E-4	0.0	0.0	3.2056725529867216E-5	3.205672552986722E-6
14-15	7.180706518690257E-4	1.602836276493361E-6	0.0	3.68652343593473E-5	4.8085088294800825E-6
16-17	8.158436647351208E-4	3.205672552986722E-6	0.0	4.1673743188827386E-5	6.411345105973444E-6
18-19	9.440705668545896E-4	3.205672552986722E-6	0.0	4.327657946532075E-5	6.411345105973444E-6
20-21	0.001025815216955751	4.8085088294800825E-6	0.0	5.609926967726764E-5	6.411345105973444E-6
22-23	0.0011299995749278196	6.411345105973444E-6	0.0	5.930494223025436E-5	6.411345105973444E-6
24-25	0.0012069357161995009	8.014181382466804E-6	0.0	6.411345105973443E-5	6.411345105973444E-6
26-27	0.00134477963597793	9.617017658960165E-6	0.0	7.37304687186946E-5	6.411345105973444E-6
28-29	0.001557956860751547	9.617017658960165E-6	0.0	8.334748637765477E-5	6.411345105973444E-6
30-31	0.0017967794659490576	9.617017658960165E-6	0.0	8.65531589306415E-5	9.617017658960165E-6
32-33	0.002175048827201491	9.617017658960165E-6	0.0	9.296450403661494E-5	1.4425526488440248E-5
34-35	0.0027248216700387134	1.1219853935453526E-5	0.0	9.296450403661494E-5	1.763119904142697E-5
36-37	0.003340310800212164	1.2822690211946887E-5	0.0	9.617017658960166E-5	1.923403531792033E-5
38-39	0.004209048062071567	1.2822690211946887E-5	0.0	1.0739003052505518E-4	1.923403531792033E-5
40-41	0.005335841964446399	1.2822690211946887E-5	0.0	1.105957030780419E-4	1.923403531792033E-5
42-43	0.006643756366064981	1.2822690211946887E-5	0.0	1.1219853935453526E-4	2.2439707870907052E-5
44-45	0.008272238022982235	1.2822690211946887E-5	0.0	1.1540421190752199E-4	2.5645380423893775E-5
46-47	0.01021968409892167	1.6028362764933608E-5	0.0	1.2822690211946886E-4	2.8851052976880497E-5
48-49	0.012473271903671335	1.6028362764933608E-5	0.0	1.314325746724556E-4	2.8851052976880497E-5
50-51	0.015467370068160933	1.6028362764933608E-5	0.0	1.314325746724556E-4	3.365956180636058E-5
52-53	0.019102602743247876	1.6028362764933608E-5	0.0	1.394467560549224E-4	3.526239808285394E-5
54-55	0.023532842211475523	1.923403531792033E-5	0.0	1.5226944626686929E-4	3.68652343593473E-5
56-57	0.028831818941562577	1.923403531792033E-5	0.0	1.6188646392582945E-4	3.846807063584066E-5
58-59	0.035321703025084195	1.923403531792033E-5	0.0	1.650921364788162E-4	3.846807063584066E-5
60-61	0.043590735375513445	1.923403531792033E-5	0.0	1.6669497275530954E-4	4.007090691233402E-5
62-63	0.05476250422267217	1.923403531792033E-5	0.0	1.73106317861283E-4	4.1673743188827386E-5
64-65	0.06896203079612687	1.923403531792033E-5	0.0	1.73106317861283E-4	4.1673743188827386E-5
66-67	0.08791236409310786	2.083687159441369E-5	0.0	1.73106317861283E-4	4.4879415741814105E-5
68-69	0.11266496471099482	2.2439707870907052E-5	0.0	1.73106317861283E-4	4.4879415741814105E-5
70-71	0.14510797378349694	2.2439707870907052E-5	0.0	1.763119904142697E-4	4.4879415741814105E-5
72-73	0.18779951800789763	2.2439707870907052E-5	0.0	1.8272333552024316E-4	4.4879415741814105E-5
74-75	0.24503039009636957	2.2439707870907052E-5	0.0	1.8913468062621658E-4	4.648225201830747E-5
76-77	0.3197305747623427	2.2439707870907052E-5	0.0	1.9554602573219003E-4	4.808508829480083E-5
78-79	0.41473709221521016	2.2439707870907052E-5	0.0	2.0035453456167013E-4	4.808508829480083E-5
80-81	0.5360557699829926	2.2439707870907052E-5	0.0	2.0195737083816349E-4	4.808508829480083E-5
82-83	0.6929269591996744	2.2439707870907052E-5	0.0	2.0676587966764355E-4	4.808508829480083E-5
84-85	0.8864181488253999	2.2439707870907052E-5	0.0	2.1317722477361703E-4	4.808508829480083E-5
86-87	1.132294836475758	2.2439707870907052E-5	0.0	2.292055875385506E-4	5.289359712428091E-5
88-89	1.428648044145445	2.5645380423893775E-5	0.0	2.3561693264452407E-4	5.7702105953760994E-5
90-91	1.7825158259245433	2.5645380423893775E-5	0.0	2.4042544147400414E-4	6.090777850674771E-5
92-93	2.2024092424412327	2.5645380423893775E-5	0.0	2.4363111402699088E-4	6.731912361272116E-5
94-95	2.694300461661727	2.5645380423893775E-5	0.0	2.5164529540945763E-4	6.731912361272116E-5
96-97	3.259378788103185	2.5645380423893775E-5	0.0	2.596594767919245E-4	6.731912361272116E-5
98-99	3.9086621183302217	2.5645380423893775E-5	0.0	2.612623130684178E-4	6.731912361272116E-5
100-101	4.639821531233093	2.5645380423893775E-5	0.0	2.644679856214046E-4	7.052479616570788E-5
102-103	5.449938261952302	2.5645380423893775E-5	0.0	2.660708218978979E-4	7.052479616570788E-5
104-105	6.347154718772438	2.5645380423893775E-5	0.0	2.70879330727378E-4	7.052479616570788E-5
106-107	7.3254346202762255	2.5645380423893775E-5	0.0	2.7248216700387134E-4	7.052479616570788E-5
108-109	8.387807327026238	2.7248216700387134E-5	0.0	2.756878395568581E-4	7.052479616570788E-5
110-111	9.508853458513563	3.0453889253373856E-5	0.0	2.788935121098448E-4	7.212763244220124E-5
112-113	10.693681253951393	3.2056725529867216E-5	0.0	2.9171620232179167E-4	7.373046871869461E-5
114-115	11.937579977523106	3.526239808285394E-5	0.0	2.9331903859828505E-4	7.373046871869461E-5
116-117	13.239462906233245	4.1673743188827386E-5	0.0	2.965247111512718E-4	7.373046871869461E-5
118-119	14.585484740325457	4.1673743188827386E-5	0.0	3.0133321998075186E-4	7.373046871869461E-5
120-121	15.952931686925556	4.4879415741814105E-5	0.0	3.061417288102319E-4	7.373046871869461E-5
122-123	17.333831238394264	4.4879415741814105E-5	0.0	3.1095023763971205E-4	7.373046871869461E-5
124-125	18.73474220077499	4.4879415741814105E-5	0.0	3.1415591019269876E-4	7.533330499518796E-5
126-127	20.145044180899692	4.4879415741814105E-5	0.0	3.1415591019269876E-4	7.693614127168132E-5
128-129	21.58219206581938	4.4879415741814105E-5	0.0	3.205672552986722E-4	7.693614127168132E-5
130-131	23.032218740220696	4.4879415741814105E-5	0.0	3.317871092341257E-4	7.693614127168132E-5
132-133	24.454679836338876	4.4879415741814105E-5	0.0	3.3499278178711247E-4	7.853897754817469E-5
134-135	25.85673522382102	4.4879415741814105E-5	0.0	3.430069631695792E-4	8.014181382466805E-5
136-137	27.230736167955698	4.4879415741814105E-5	3.205672552986722E-6	3.430069631695792E-4	8.014181382466805E-5
138-139	28.58494528974824	4.648225201830747E-5	4.8085088294800825E-6	3.446097994460726E-4	8.17446501011614E-5
>>END_MODULE
