FastQCFastQC Report
Mon 24 Jun 2019
SRR8261619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8261619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31194702
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG1437740.4608923656331129TruSeq Adapter, Index 13 (97% over 37bp)
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG385310.12351776913913139No Hit
CATTAATCTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCT374060.11991138751702131No Hit
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC366810.11758727491610595No Hit
GCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGCACTCTTCATC340450.10913712206643295No Hit
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT322840.10349193270062333No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)