##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261627_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28444252
Sequences flagged as poor quality	0
Sequence length	150
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88903670238894	32.0	32.0	32.0	32.0	32.0
2	31.381410697669253	32.0	32.0	32.0	32.0	32.0
3	35.08232643980232	37.0	37.0	37.0	32.0	37.0
4	35.71595603920258	37.0	37.0	37.0	32.0	37.0
5	35.90587680069773	37.0	37.0	37.0	37.0	37.0
6	39.25591634471527	41.0	41.0	41.0	37.0	41.0
7	39.40221142043039	41.0	41.0	41.0	37.0	41.0
8	39.432983788780945	41.0	41.0	41.0	37.0	41.0
9	39.467807977513345	41.0	41.0	41.0	37.0	41.0
10-14	39.48389980513462	41.0	41.0	41.0	37.0	41.0
15-19	39.44520219410235	41.0	41.0	41.0	37.0	41.0
20-24	39.40417476965117	41.0	41.0	41.0	37.0	41.0
25-29	39.37224838255546	41.0	41.0	41.0	37.0	41.0
30-34	39.34721017800011	41.0	41.0	41.0	37.0	41.0
35-39	39.23814580183019	41.0	41.0	41.0	37.0	41.0
40-44	39.18709768145776	41.0	41.0	41.0	37.0	41.0
45-49	39.123947903428785	41.0	41.0	41.0	37.0	41.0
50-54	39.04550208597505	41.0	41.0	41.0	37.0	41.0
55-59	38.986061331477444	41.0	41.0	41.0	37.0	41.0
60-64	38.93430494850067	41.0	41.0	41.0	35.0	41.0
65-69	38.877569056834396	41.0	41.0	41.0	32.0	41.0
70-74	38.82131233403501	41.0	41.0	41.0	32.0	41.0
75-79	38.340549654812506	41.0	39.4	41.0	31.0	41.0
80-84	38.81404920052037	41.0	41.0	41.0	32.0	41.0
85-89	38.78861302452249	41.0	41.0	41.0	32.0	41.0
90-94	38.62783013594451	41.0	41.0	41.0	32.0	41.0
95-99	38.47344333048378	41.0	41.0	41.0	32.0	41.0
100-104	38.28097858224572	41.0	41.0	41.0	32.0	41.0
105-109	38.09157957818684	41.0	39.4	41.0	32.0	41.0
110-114	37.82415451810791	41.0	37.0	41.0	32.0	41.0
115-119	37.502800094725636	41.0	37.0	41.0	27.0	41.0
120-124	37.21892663586302	41.0	37.0	41.0	27.0	41.0
125-129	36.82101356716991	41.0	37.0	41.0	27.0	41.0
130-134	36.24802040848182	41.0	37.0	41.0	22.0	41.0
135-139	35.94412263679847	41.0	37.0	41.0	22.0	41.0
140-144	35.537434719675524	41.0	32.0	41.0	22.0	41.0
145-149	35.194481015004364	41.0	32.0	41.0	22.0	41.0
150	29.419629667181965	32.0	22.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	22.0
12	103.0
13	611.0
14	5448.0
15	26894.0
16	60216.0
17	78428.0
18	81967.0
19	83864.0
20	89544.0
21	98009.0
22	109438.0
23	124211.0
24	143829.0
25	172599.0
26	226206.0
27	261213.0
28	228058.0
29	253430.0
30	291214.0
31	341140.0
32	402624.0
33	482712.0
34	593825.0
35	750174.0
36	988684.0
37	1387284.0
38	2197347.0
39	4716116.0
40	1.4249042E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.654913625325385	20.31319212770813	12.731295031698822	37.30059921526766
2	25.253931629939082	25.789668939967893	29.827047979687794	19.129351450405235
3	21.59909428010308	26.152813197134563	25.597127054124446	26.650965468637906
4	24.41264425250719	32.85790078396835	21.007309310499988	21.722145653024473
5	26.621775816077005	34.00618163557263	19.936094645765337	19.43594790258503
6	21.66742862494679	33.587278020177855	21.749877620265774	22.99541573460958
7	21.673240474084764	20.479742158997922	32.54592254954227	25.30109481737505
8	21.43520776198534	23.115020522911934	26.56449256212096	28.88527915298177
9	21.586656594098518	24.113574862154927	28.025022419292306	26.274746124454246
10-14	24.87938025620109	26.240660950175997	23.248958019157616	25.631000774465296
15-19	24.708102009502657	26.372202721309034	23.932857858241448	24.98683741094686
20-24	24.63003635929281	26.573075234428234	24.041081010053514	24.755807396225446
25-29	24.38335658994335	26.409843988465433	24.21232514431831	24.99447427727291
30-34	24.244599051654653	26.340539135340325	24.3016926839178	25.113169129087225
35-39	24.060843494504617	26.257127020811595	24.380319062970855	25.30171042171294
40-44	24.21943066583399	26.27555550092948	24.486518645775185	25.018495187461344
45-49	24.134271938736443	26.24060888928233	24.424994267721353	25.200124904259873
50-54	24.42885154807703	26.2009206203069	24.27914499572954	25.09108283588653
55-59	24.391091555013187	26.083084826789616	24.215966433723622	25.30985718447358
60-64	24.16841013054161	26.214121364098396	24.569734247139312	25.047734258220682
65-69	24.23613211631061	26.278346577759574	24.642232301405432	24.843289004524383
70-74	24.298211044041548	26.786852717675718	24.196365322906573	24.71857091537616
75-79	24.22764118914364	26.882929496223362	24.139563605438187	24.74986570919481
80-84	24.268378723488034	26.98478752457481	24.084943059567372	24.661890692369784
85-89	24.536515439074304	26.847847527172604	23.735061590013217	24.880575443739875
90-94	24.688303020612594	27.000453941975582	23.744650748040968	24.566592289370853
95-99	24.894065075702336	27.19520422010136	23.479615417519202	24.431115286677098
100-104	25.515769032854102	27.38870449983541	23.076382856025564	24.019143611284925
105-109	25.934494315693247	27.730188620381035	22.73019568687678	23.605121377048942
110-114	26.541301483943585	27.709450725434152	22.475392529825406	23.273855260796854
115-119	27.236952209870807	27.64588207342632	22.363991278094176	22.7531744386087
120-124	27.869418732436905	27.571411751549075	22.261849704296825	22.297319811717195
125-129	28.54728445203439	27.521429331064557	22.189570235629034	21.741715981272023
130-134	29.262253993216436	27.205524433714768	22.20615756748774	21.326064005581056
135-139	29.63328106887666	26.968651598744565	22.459437881498417	20.93862945088036
140-144	29.9241435492837	26.66080795515382	22.5724135758606	20.842634919701876
145-149	30.310283170692635	26.086383163673975	22.942155982713704	20.661177682919682
150	29.98763158948507	25.95992643430724	23.301444155454057	20.75099782075364
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	62.0
1	49.0
2	46.5
3	69.0
4	75.5
5	95.0
6	119.5
7	147.0
8	172.5
9	187.5
10	239.0
11	284.5
12	323.5
13	401.0
14	453.5
15	524.0
16	633.0
17	784.0
18	1024.0
19	1382.0
20	2057.0
21	2954.5
22	4398.5
23	6361.0
24	9247.0
25	13590.5
26	20423.0
27	30558.5
28	43879.5
29	65688.0
30	91226.0
31	122069.0
32	167931.5
33	226865.5
34	284650.0
35	333900.5
36	396615.0
37	471943.5
38	519955.5
39	553447.0
40	598248.5
41	674852.5
42	771227.0
43	862806.0
44	941304.5
45	1024812.0
46	1120646.0
47	1181384.0
48	1233620.5
49	1321411.5
50	1438305.0
51	1465083.5
52	1427746.0
53	1400189.0
54	1302825.0
55	1218546.5
56	1144489.5
57	1023739.5
58	914054.0
59	788830.0
60	659579.5
61	545771.0
62	443101.0
63	362638.5
64	284371.0
65	214147.5
66	161841.0
67	124658.5
68	100141.5
69	80016.5
70	60006.5
71	45253.0
72	37455.0
73	28671.5
74	20311.5
75	14639.5
76	10096.5
77	6861.5
78	4698.0
79	3362.0
80	2296.0
81	1541.0
82	1049.5
83	671.0
84	467.5
85	340.5
86	257.5
87	198.0
88	156.0
89	128.5
90	96.0
91	73.5
92	69.5
93	62.0
94	53.5
95	42.5
96	40.5
97	40.0
98	32.5
99	25.5
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.7755573252550287
2	0.013433294009629785
3	4.851595324074614E-4
4	4.1836220548179643E-4
5	0.0
6	0.0
7	1.9687633199143363E-4
8	6.644576204710885E-4
9	0.0
10-14	3.9023701519730596E-4
15-19	0.0
20-24	8.578183036769608E-5
25-29	3.164083907005183E-5
30-34	3.487523595276824E-4
35-39	2.59454880374425E-4
40-44	0.0012874305852725535
45-49	2.320328198470468E-4
50-54	0.0016650112648418387
55-59	4.6336250993698124E-4
60-64	3.881276259259692E-4
65-69	8.310993729066947E-4
70-74	1.8492312612052516E-4
75-79	3.9375266398286726E-5
80-84	0.001729699202496167
85-89	5.05550295363717E-4
90-94	3.093770931293957E-4
95-99	1.5539167632181014E-4
100-104	1.6593862267849405E-4
105-109	5.899258662171886E-4
110-114	1.5468854656469785E-4
115-119	3.5156487855613146E-5
120-124	6.679732692566498E-5
125-129	6.222698350443527E-4
130-134	9.210999818170645E-5
135-139	2.2711091154726094E-4
140-144	0.0
145-149	2.7984564333068064E-4
150	5.273473178341972E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.8444252E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.60654608303007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.01377689562052	24.87347404021777
2	12.278454816255586	8.252729152217917
3	4.44666451106522	4.48311107420663
4	2.3289441410580496	3.130710744050809
5	1.4019943441734266	2.3558093767805888
6	0.9593244319077366	1.9343748437690396
7	0.6841396038593872	1.6094098387028501
8	0.48326019700937023	1.299256486471167
9	0.38867440791779106	1.1755803960885305
>10	2.5221547118549204	16.267084396508317
>50	0.2643791457653815	6.198180514330702
>100	0.1928965052853785	12.89426748511468
>500	0.021202723401720056	4.913384260549348
>1k	0.013448543791325136	8.343063182806638
>5k	5.871608865017123E-4	1.2863985714840434
>10k+	9.786014774861045E-5	0.9831656367011921
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC	162796	0.5723335596942398	Illumina Single End PCR Primer 1 (96% over 32bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.273473178341972E-5	0.0	0.0	3.515648785561315E-6	0.0
2	5.976602935454235E-5	3.515648785561315E-6	0.0	3.515648785561315E-6	0.0
3	8.085992206791024E-5	3.515648785561315E-6	0.0	7.03129757112263E-6	0.0
4	1.687511417069431E-4	3.515648785561315E-6	0.0	7.03129757112263E-6	0.0
5	2.5312671256041465E-4	3.515648785561315E-6	0.0	7.03129757112263E-6	0.0
6	3.1992403948607963E-4	3.515648785561315E-6	0.0	1.406259514224526E-5	3.515648785561315E-6
7	3.339866346283249E-4	3.515648785561315E-6	0.0	2.109389271336789E-5	3.515648785561315E-6
8	3.6211182491281544E-4	3.515648785561315E-6	0.0	2.109389271336789E-5	3.515648785561315E-6
9	5.80082049617617E-4	3.515648785561315E-6	0.0	2.109389271336789E-5	3.515648785561315E-6
10-11	7.857475035729539E-4	3.515648785561315E-6	0.0	2.109389271336789E-5	3.515648785561315E-6
12-13	0.0010582102844539558	3.515648785561315E-6	0.0	2.4609541498929204E-5	5.273473178341972E-6
14-15	0.0012427818456959248	3.515648785561315E-6	0.0	3.339866346283249E-5	7.03129757112263E-6
16-17	0.0013570404312266676	3.515648785561315E-6	0.0	3.5156487855613146E-5	8.789121963903287E-6
18-19	0.0015011820314346815	3.515648785561315E-6	0.0	3.867213664117446E-5	1.0546946356683944E-5
20-21	0.0015943467242520563	7.03129757112263E-6	0.0	4.218778542673578E-5	1.0546946356683944E-5
22-23	0.0016804801194983086	7.03129757112263E-6	0.0	4.746125860507775E-5	1.0546946356683944E-5
24-25	0.0018140747733496384	7.03129757112263E-6	0.0	5.273473178341972E-5	1.0546946356683944E-5
26-27	0.0019652476711287748	7.03129757112263E-6	0.0	5.800820496176169E-5	1.2304770749464602E-5
28-29	0.0021673974762985507	7.03129757112263E-6	0.0	6.328167814010366E-5	1.406259514224526E-5
30-31	0.0024890793401774105	7.03129757112263E-6	0.0	6.855515131844563E-5	1.406259514224526E-5
32-33	0.0029267776139797947	7.03129757112263E-6	0.0	7.207080010400695E-5	1.406259514224526E-5
34-35	0.003473461000134579	7.03129757112263E-6	0.0	7.734427328234892E-5	1.406259514224526E-5
36-37	0.004132645147427326	7.03129757112263E-6	0.0	8.261774646069089E-5	1.406259514224526E-5
38-39	0.004927181772964182	7.03129757112263E-6	0.0	8.613339524625221E-5	1.933606832058723E-5
40-41	0.006038126789201558	8.789121963903287E-6	0.0	8.964904403181354E-5	2.109389271336789E-5
42-43	0.007356495083787051	1.406259514224526E-5	0.0	9.140686842459418E-5	2.4609541498929204E-5
44-45	0.008973693525145255	1.406259514224526E-5	0.0	9.140686842459418E-5	2.636736589170986E-5
46-47	0.010979371157307986	1.406259514224526E-5	0.0	9.843816599571681E-5	2.812519028449052E-5
48-49	0.013616107746478971	1.406259514224526E-5	0.0	1.0195381478127812E-4	3.164083907005183E-5
50-51	0.016868082873123188	1.406259514224526E-5	0.0	1.1074293674518141E-4	3.5156487855613146E-5
52-53	0.020956782410730998	1.406259514224526E-5	0.0	1.1777423431630405E-4	3.5156487855613146E-5
54-55	0.02585056552023235	1.406259514224526E-5	0.0	1.28321180672988E-4	3.5156487855613146E-5
56-57	0.03202052913889246	1.406259514224526E-5	0.0	1.4238377581523323E-4	3.5156487855613146E-5
58-59	0.03986921505265809	1.406259514224526E-5	0.0	1.5117289777913655E-4	3.5156487855613146E-5
60-61	0.049721820774193674	1.406259514224526E-5	0.0	1.5468854656469785E-4	3.5156487855613146E-5
62-63	0.06300218406165153	1.7578243927806573E-5	0.0	1.5996201974303982E-4	3.5156487855613146E-5
64-65	0.08046968505271293	1.7578243927806573E-5	0.0	1.617198441358205E-4	3.5156487855613146E-5
66-67	0.10315440884154732	1.7578243927806573E-5	0.0	1.6523549292138178E-4	3.867213664117446E-5
68-69	0.13372824850518128	1.7578243927806573E-5	0.0	1.6523549292138178E-4	4.746125860507775E-5
70-71	0.17291894334222604	1.7578243927806573E-5	0.0	1.7050896609972375E-4	4.921908299785841E-5
72-73	0.22549019745711718	1.7578243927806573E-5	0.0	1.810559124564077E-4	5.097690739063906E-5
74-75	0.29618462106157684	2.109389271336789E-5	0.0	1.8281373684918837E-4	5.273473178341972E-5
76-77	0.3878059440620903	2.109389271336789E-5	0.0	1.8808721002753034E-4	5.273473178341972E-5
78-79	0.5047434539674307	2.2851717106148546E-5	0.0	1.9160285881309166E-4	5.273473178341972E-5
80-81	0.6527557834883477	2.4609541498929204E-5	0.0	1.933606832058723E-4	5.273473178341972E-5
82-83	0.840915064315982	2.4609541498929204E-5	0.0	1.9687633199143363E-4	5.273473178341972E-5
84-85	1.0759871625381465	2.4609541498929204E-5	0.0	1.9687633199143363E-4	5.625038056898104E-5
86-87	1.3693065298394909	2.4609541498929204E-5	0.0	1.9863415638421428E-4	5.976602935454235E-5
88-89	1.7211192616350046	2.812519028449052E-5	0.0	2.0918110274089822E-4	5.976602935454235E-5
90-91	2.138092785846504	2.812519028449052E-5	0.0	2.179702247048015E-4	5.976602935454235E-5
92-93	2.6290601700477128	2.812519028449052E-5	0.0	2.179702247048015E-4	5.976602935454235E-5
94-95	3.201921077059787	2.812519028449052E-5	0.0	2.179702247048015E-4	5.976602935454235E-5
96-97	3.8597481839213064	3.164083907005183E-5	0.0	2.2851717106148547E-4	5.976602935454235E-5
98-99	4.6026750852861245	3.164083907005183E-5	0.0	2.3203281984704677E-4	6.152385374732301E-5
100-101	5.431768780560656	3.164083907005183E-5	0.0	2.3379064423982744E-4	6.328167814010366E-5
102-103	6.342516934528636	3.164083907005183E-5	0.0	2.355484686326081E-4	6.503950253288432E-5
104-105	7.345842667966801	3.164083907005183E-5	0.0	2.355484686326081E-4	6.679732692566498E-5
106-107	8.42830565556795	3.164083907005183E-5	0.0	2.355484686326081E-4	6.679732692566498E-5
108-109	9.593741470157134	3.5156487855613146E-5	0.0	2.408219418109501E-4	7.031297571122629E-5
110-111	10.807471752113573	3.867213664117446E-5	0.0	2.4961106377485335E-4	7.031297571122629E-5
112-113	12.083864958023856	3.867213664117446E-5	0.0	2.5312671256041465E-4	7.031297571122629E-5
114-115	13.418347228817971	3.867213664117446E-5	0.0	2.5312671256041465E-4	7.031297571122629E-5
116-117	14.796797961148705	3.867213664117446E-5	0.0	2.56642361345976E-4	7.031297571122629E-5
118-119	16.204780143278157	3.867213664117446E-5	0.0	2.56642361345976E-4	7.031297571122629E-5
120-121	17.613766043135886	4.394560981951643E-5	0.0	2.584001857387566E-4	7.382862449678761E-5
122-123	19.03754403525886	4.570343421229709E-5	0.0	2.6718930770265993E-4	7.382862449678761E-5
124-125	20.471524440157538	4.570343421229709E-5	0.0	2.777362540593439E-4	7.382862449678761E-5
126-127	21.908802523617073	4.570343421229709E-5	0.0	2.8125190284490517E-4	7.382862449678761E-5
128-129	23.357355292731903	4.746125860507775E-5	0.0	2.935566735943698E-4	7.382862449678761E-5
130-131	24.790189596126485	4.921908299785841E-5	0.0	2.9531449798715046E-4	7.558644888956828E-5
132-133	26.210631237551965	5.097690739063906E-5	0.0	3.005879711654924E-4	8.085992206791024E-5
134-135	27.622860323414375	5.273473178341972E-5	0.0	3.18166215093299E-4	8.085992206791024E-5
136-137	29.007463441119846	5.273473178341972E-5	0.0	3.1992403948607963E-4	8.085992206791024E-5
138-139	30.37311369622235	5.4492556176200384E-5	0.0	3.3222881023554424E-4	8.437557085347157E-5
>>END_MODULE
