##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261627_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28444252
Sequences flagged as poor quality	0
Sequence length	150
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45520943915136	32.0	32.0	32.0	32.0	32.0
2	31.64221171996367	32.0	32.0	32.0	32.0	32.0
3	35.736072933118436	37.0	37.0	37.0	32.0	37.0
4	36.27594176144973	37.0	37.0	37.0	37.0	37.0
5	36.49861065075643	37.0	37.0	37.0	37.0	37.0
6	40.02752000650254	41.0	41.0	41.0	37.0	41.0
7	40.086393939977754	41.0	41.0	41.0	37.0	41.0
8	40.22889077905793	41.0	41.0	41.0	37.0	41.0
9	40.225335016719725	41.0	41.0	41.0	41.0	41.0
10-14	40.25697523703559	41.0	41.0	41.0	41.0	41.0
15-19	40.24815988833175	41.0	41.0	41.0	41.0	41.0
20-24	40.23864199346849	41.0	41.0	41.0	41.0	41.0
25-29	40.17401140307715	41.0	41.0	41.0	41.0	41.0
30-34	40.15565813437456	41.0	41.0	41.0	41.0	41.0
35-39	40.14096684982259	41.0	41.0	41.0	41.0	41.0
40-44	40.05172903826052	41.0	41.0	41.0	37.0	41.0
45-49	40.02287280396756	41.0	41.0	41.0	37.0	41.0
50-54	40.05109984962867	41.0	41.0	41.0	37.0	41.0
55-59	40.02249707251925	41.0	41.0	41.0	37.0	41.0
60-64	39.98399674563423	41.0	41.0	41.0	37.0	41.0
65-69	39.945432152689406	41.0	41.0	41.0	37.0	41.0
70-74	39.90001570088747	41.0	41.0	41.0	37.0	41.0
75-79	39.43088711209562	41.0	40.2	41.0	36.0	41.0
80-84	39.78872133463028	41.0	41.0	41.0	37.0	41.0
85-89	39.774640760460144	41.0	41.0	41.0	37.0	41.0
90-94	39.72905780050043	41.0	41.0	41.0	37.0	41.0
95-99	39.6792779082396	41.0	41.0	41.0	37.0	41.0
100-104	39.619418714192236	41.0	41.0	41.0	37.0	41.0
105-109	39.51218980200288	41.0	41.0	41.0	37.0	41.0
110-114	39.42427186343308	41.0	41.0	41.0	37.0	41.0
115-119	39.32409613724418	41.0	41.0	41.0	37.0	41.0
120-124	39.19029275229315	41.0	41.0	41.0	37.0	41.0
125-129	39.02551160072693	41.0	41.0	41.0	37.0	41.0
130-134	38.82206158207289	41.0	41.0	41.0	32.0	41.0
135-139	38.584310362599794	41.0	41.0	41.0	32.0	41.0
140-144	38.32964951231623	41.0	41.0	41.0	32.0	41.0
145-149	38.046585524555184	41.0	39.4	41.0	31.0	41.0
150	34.245833042120424	37.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	6.0
13	21.0
14	30.0
15	30.0
16	69.0
17	122.0
18	393.0
19	1104.0
20	2566.0
21	5460.0
22	10610.0
23	19980.0
24	35459.0
25	65472.0
26	149911.0
27	121820.0
28	114525.0
29	145656.0
30	183284.0
31	226339.0
32	275876.0
33	333737.0
34	408789.0
35	508582.0
36	657539.0
37	905823.0
38	1378940.0
39	2740596.0
40	2.0151513E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.208161340115744	9.59148192268705	10.35794123852165	38.84241549867555
2	25.86093684947089	11.202940874605071	38.014385746162965	24.921736529761073
3	25.47152233076827	14.931213518991465	23.581565794031075	36.01569835620919
4	28.04842846868638	23.488452911411965	23.76019503263982	24.702923587261836
5	28.741785378126878	26.709160083642917	26.096308607057967	18.452745931172238
6	22.736801893641005	27.454967965231358	28.84738641913967	20.960843721987967
7	19.70122547570217	21.719920760310625	41.16717801106354	17.411675752923657
8	20.836725262443466	20.753128675566458	34.689187376697404	23.720958685292672
9	20.240244735652883	21.797746441280324	35.50047570534717	22.46153311771962
10-14	25.228240234866796	24.77104151612178	26.925268232958576	23.075450016052848
15-19	25.51482293081453	23.715070697088812	27.072933155953223	23.697173216143437
20-24	25.035020671806407	24.201742188553432	27.127059807433078	23.636177332207076
25-29	24.931726269486614	24.284453261034024	26.93321076974449	23.85060969973487
30-34	24.808357444427294	24.462470873183484	26.35763914045628	24.371532541932943
35-39	25.096524469380512	24.32118487653556	26.62566579959278	23.95662485449115
40-44	24.860195997606933	24.053873140371586	27.163557771949808	23.922373090071673
45-49	25.015766781817668	24.003775186976437	27.063432522439296	23.9170255087666
50-54	24.959332899446864	24.331936607593423	26.619984718182987	24.088745774776722
55-59	24.992461196378848	24.13603088553235	26.690832745888155	24.180675172200647
60-64	24.798885807166926	24.099497401993094	26.762612424547527	24.339004366292453
65-69	24.92458897966013	25.218569524515143	26.128867439486875	23.727974056337857
70-74	24.99383529329639	24.964690486582427	26.157169413621535	23.88430480649965
75-79	25.024635394543544	24.754675092850754	26.182819919284306	24.0378695933214
80-84	24.955516725825703	24.801055331502518	26.239391633950067	24.00403630872172
85-89	25.336304378649576	24.61209608639277	26.14424321648879	23.90735631846886
90-94	25.291939262669093	24.823686815699208	25.94485972687443	23.939514194757265
95-99	25.532432124948514	24.98455117547055	25.761211982117022	23.721804717463915
100-104	25.53667411305014	25.206354596308028	25.333428690468622	23.92354260017321
105-109	25.66622431749846	25.4472238263658	24.853877151329858	24.03267470480588
110-114	25.902522020510567	25.778753968618158	24.480180158807023	23.838543852064255
115-119	25.673928842367445	26.195034503363747	24.079709966444423	24.051326687824385
120-124	25.660043285109445	26.24864182508165	23.78679393036448	24.304520959444428
125-129	25.27937143974931	26.44258647294937	23.65718175874749	24.620860328553825
130-134	25.01345282241378	26.310403271076154	23.79986905598421	24.876274850525856
135-139	24.745123524406385	26.388506613785857	23.812424071164315	25.053945790643446
140-144	24.44902033923287	26.33570750679133	24.078140684527508	25.137131469448292
145-149	24.106571806153614	26.108249352021097	24.53298772114483	25.252191120680457
150	23.740227023723456	25.855062035029082	24.866268235846036	25.538442705401426
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	15.5
2	10.0
3	13.5
4	19.0
5	20.5
6	15.5
7	22.5
8	25.5
9	25.5
10	36.5
11	45.5
12	52.5
13	76.0
14	109.5
15	144.5
16	197.0
17	280.0
18	431.0
19	679.0
20	1252.5
21	2177.5
22	3285.0
23	4993.0
24	7885.0
25	12404.5
26	18806.0
27	27445.0
28	41366.5
29	62921.0
30	88448.5
31	116271.5
32	157529.5
33	210885.5
34	268992.0
35	330917.5
36	399388.5
37	489836.5
38	560091.5
39	579440.5
40	603312.5
41	687613.0
42	768126.0
43	853935.0
44	963880.5
45	1084244.0
46	1164924.0
47	1203000.0
48	1254450.0
49	1339373.0
50	1469022.5
51	1480571.0
52	1433910.5
53	1418191.0
54	1318853.0
55	1206262.0
56	1105376.5
57	991703.0
58	909614.0
59	811839.5
60	678650.5
61	544509.5
62	433957.0
63	335868.0
64	257390.5
65	197489.5
66	143848.0
67	108881.5
68	82059.5
69	59215.5
70	42482.5
71	29293.0
72	22795.0
73	16817.0
74	11473.0
75	8369.5
76	6215.5
77	3968.0
78	2306.0
79	1399.0
80	931.0
81	621.0
82	415.0
83	240.5
84	122.5
85	67.5
86	27.5
87	15.5
88	9.0
89	6.0
90	6.5
91	6.0
92	3.5
93	2.5
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	3.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0162756257397805
2	0.01216766044682771
3	0.0
4	0.019423959540226264
5	1.0546946356683944E-5
6	3.5156487855613144E-6
7	0.003269553370572023
8	0.0017824339342795866
9	6.117228886876688E-4
10-14	2.735174755166703E-4
15-19	9.000060891036966E-5
20-24	1.821106070920761E-4
25-29	0.04712656884069231
30-34	0.0012684460818305225
35-39	0.004601281130542648
40-44	0.03797885070066177
45-49	0.10576196554579814
50-54	8.866466237185636E-4
55-59	0.00262548651305719
60-64	0.0017093084395399113
65-69	0.0016347766852860114
70-74	2.6859556721688446E-4
75-79	7.664114352523665E-4
80-84	0.0022493120930021293
85-89	0.001374618675154474
90-94	8.303962431495826E-4
95-99	0.002334390793612713
100-104	0.003529008250946448
105-109	7.249267795827432E-4
110-114	0.0025467359802606167
115-119	1.3992282166534032E-4
120-124	0.001345087225355759
125-129	0.0011531328016641113
130-134	1.2304770749464603E-4
135-139	0.0018028246972358422
140-144	0.0014632130245506192
145-149	0.013700483317332444
150	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.8444252E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.827213521779772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.28142379337955	21.261262476216043
2	13.10578534097149	7.818181154715359
3	4.996081977822927	4.470576117745207
4	2.6309462573543496	3.1389518312974216
5	1.593123028074369	2.3759210362419276
6	1.1195434161523998	2.0035716312288225
7	0.7785485946939991	1.6255354619713638
8	0.578046768320897	1.3793219507425725
9	0.4493825354993858	1.2063445955369063
>10	2.8959137209294696	16.586908373423
>50	0.30511000750195877	6.338933863353928
>100	0.22427252380758908	13.302097228928053
>500	0.024179182024125574	4.967566786093807
>1k	0.01602279126126338	8.957631616446049
>5k	0.0012518662503157286	2.523789102922115
>10k+	3.6819595597368447E-4	2.043406773137541
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG	157661	0.5542807031803825	TruSeq Adapter, Index 7 (97% over 34bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.789121963903288E-5	0.0	0.0	1.406259514224526E-5	0.0
2	1.1601640992352338E-4	0.0	0.0	3.5156487855613146E-5	0.0
3	1.687511417069431E-4	0.0	0.0	3.5156487855613146E-5	2.4609541498929204E-5
4	3.093770931293957E-4	0.0	0.0	4.570343421229709E-5	3.5156487855613146E-5
5	4.042996103395512E-4	0.0	0.0	5.273473178341972E-5	3.867213664117446E-5
6	4.816438836219001E-4	0.0	0.0	5.273473178341972E-5	3.867213664117446E-5
7	5.168003714775132E-4	0.0	0.0	5.273473178341972E-5	4.218778542673578E-5
8	5.625038056898103E-4	0.0	0.0	5.625038056898104E-5	5.625038056898104E-5
9	7.945366255368571E-4	0.0	0.0	5.976602935454235E-5	7.031297571122629E-5
10-11	0.0010142646746344392	0.0	0.0	6.152385374732301E-5	7.910209767512958E-5
12-13	0.0012990322262649058	0.0	0.0	6.855515131844563E-5	8.964904403181354E-5
14-15	0.0014853616118996557	0.0	0.0	7.382862449678761E-5	9.843816599571681E-5
16-17	0.0015890732510737142	0.0	0.0	7.734427328234892E-5	9.843816599571681E-5
18-19	0.0017296992024961669	0.0	1.7578243927806574E-6	8.085992206791024E-5	1.1425858553074274E-4
20-21	0.0018193482465279805	0.0	5.273473178341972E-6	8.261774646069089E-5	1.1601640992352338E-4
22-23	0.0018914190466319874	0.0	7.03129757112263E-6	9.316469281737484E-5	1.1601640992352338E-4
24-25	0.00200567763216273	0.0	7.03129757112263E-6	1.0371163917405878E-4	1.195320587090847E-4
26-27	0.002098842324980105	0.0	7.03129757112263E-6	1.1250076113796206E-4	1.3007900506576865E-4
28-29	0.0022095852617252864	1.7578243927806574E-6	7.03129757112263E-6	1.1601640992352338E-4	1.406259514224526E-4
30-31	0.0024064615937167202	3.515648785561315E-6	7.03129757112263E-6	1.1601640992352338E-4	1.4589942460079458E-4
32-33	0.0027632999454511933	3.515648785561315E-6	7.03129757112263E-6	1.1601640992352338E-4	1.529307221719172E-4
34-35	0.003243186004680313	3.515648785561315E-6	7.03129757112263E-6	1.1777423431630405E-4	1.7050896609972375E-4
36-37	0.003788111566442317	3.515648785561315E-6	7.03129757112263E-6	1.2304770749464603E-4	2.0742327834811757E-4
38-39	0.004531671284588534	5.273473178341972E-6	7.03129757112263E-6	1.3359465385132997E-4	2.548845369531953E-4
40-41	0.005556482905579658	7.03129757112263E-6	7.03129757112263E-6	1.371103026368913E-4	2.9707232237993113E-4
42-43	0.006825632117167293	7.03129757112263E-6	7.03129757112263E-6	1.371103026368913E-4	3.1289274191495704E-4
44-45	0.008332087621780317	1.0546946356683944E-5	1.0546946356683944E-5	1.371103026368913E-4	3.269553370572023E-4
46-47	0.010277999224588504	1.2304770749464602E-5	1.0546946356683944E-5	1.3886812702967194E-4	3.3222881023554424E-4
48-49	0.012863758906368851	1.406259514224526E-5	1.0546946356683944E-5	1.4238377581523323E-4	3.392601078066669E-4
50-51	0.016048936706087404	1.406259514224526E-5	1.0546946356683944E-5	1.441416002080139E-4	3.462914053777895E-4
52-53	0.020225527463334246	1.7578243927806573E-5	1.0546946356683944E-5	1.5117289777913655E-4	3.6914312248393803E-4
54-55	0.025238842631544682	1.7578243927806573E-5	1.0546946356683944E-5	1.5996201974303982E-4	3.7793224444784135E-4
56-57	0.031408806250204785	1.933606832058723E-5	1.0546946356683944E-5	1.617198441358205E-4	3.867213664117446E-4
58-59	0.03933307861285999	2.4609541498929204E-5	1.2304770749464602E-5	1.617198441358205E-4	3.9023701519730596E-4
60-61	0.049451115817705456	2.4609541498929204E-5	1.406259514224526E-5	1.7050896609972375E-4	3.9551048837564793E-4
62-63	0.0629248397883692	2.4609541498929204E-5	1.406259514224526E-5	1.7578243927806575E-4	3.9726831276842855E-4
64-65	0.0807632417263073	2.4609541498929204E-5	1.406259514224526E-5	1.775402636708464E-4	3.9726831276842855E-4
66-67	0.10419679870646625	2.4609541498929204E-5	1.406259514224526E-5	1.8281373684918837E-4	4.0957308351789317E-4
68-69	0.1356354879713483	2.4609541498929204E-5	1.406259514224526E-5	1.8457156124196904E-4	4.4297174698072566E-4
70-71	0.17590197133677482	2.812519028449052E-5	1.406259514224526E-5	1.863293856347497E-4	4.535186933374096E-4
72-73	0.22980214069260813	2.812519028449052E-5	1.406259514224526E-5	1.89845034420311E-4	4.5703434212297095E-4
74-75	0.30295927627135355	2.812519028449052E-5	1.406259514224526E-5	1.9863415638421428E-4	4.5703434212297095E-4
76-77	0.3973948761247088	2.812519028449052E-5	1.406259514224526E-5	2.021498051697756E-4	4.710969372652162E-4
78-79	0.5174964699370544	2.812519028449052E-5	1.406259514224526E-5	2.0390762956255625E-4	4.710969372652162E-4
80-81	0.6708051243534194	2.9883014677271177E-5	1.406259514224526E-5	2.0390762956255625E-4	4.710969372652162E-4
82-83	0.864508583315884	3.164083907005183E-5	1.406259514224526E-5	2.0742327834811757E-4	4.7285476165799686E-4
84-85	1.1078600344280454	3.164083907005183E-5	1.406259514224526E-5	2.1269675152645956E-4	4.7637041044355815E-4
86-87	1.4113501736660186	3.164083907005183E-5	1.406259514224526E-5	2.1972804909758215E-4	4.886751811930228E-4
88-89	1.7749983370981244	3.164083907005183E-5	1.7578243927806573E-5	2.267593466687048E-4	4.886751811930228E-4
90-91	2.2061451993886143	3.5156487855613146E-5	1.7578243927806573E-5	2.3203281984704677E-4	4.886751811930228E-4
92-93	2.7152146591866786	4.042996103395512E-5	1.7578243927806573E-5	2.3730629302538874E-4	4.886751811930228E-4
94-95	3.3111118548661427	4.570343421229709E-5	1.7578243927806573E-5	2.408219418109501E-4	4.886751811930228E-4
96-97	3.9932180322407493	4.570343421229709E-5	1.7578243927806573E-5	2.4609541498929206E-4	4.904330055858035E-4
98-99	4.76145233138843	4.570343421229709E-5	1.7578243927806573E-5	2.4961106377485335E-4	4.921908299785841E-4
100-101	5.617491716779896	4.570343421229709E-5	1.7578243927806573E-5	2.51368888167634E-4	4.921908299785841E-4
102-103	6.560355322403979	4.570343421229709E-5	1.7578243927806573E-5	2.636736589170986E-4	4.921908299785841E-4
104-105	7.597557847539813	4.570343421229709E-5	1.7578243927806573E-5	2.6894713209544055E-4	4.921908299785841E-4
106-107	8.716740028881757	4.570343421229709E-5	1.7578243927806573E-5	2.794940784521245E-4	4.957064787641454E-4
108-109	9.917794639141857	4.570343421229709E-5	1.7578243927806573E-5	2.865253760232472E-4	4.992221275497067E-4
110-111	11.1724541042598	4.570343421229709E-5	1.7578243927806573E-5	2.9004102480880843E-4	5.115268982991714E-4
112-113	12.489106762237938	4.570343421229709E-5	1.7578243927806573E-5	3.005879711654924E-4	5.168003714775132E-4
114-115	13.866958076450736	4.570343421229709E-5	1.7578243927806573E-5	3.041036199510537E-4	5.220738446558553E-4
116-117	15.289554810581766	4.570343421229709E-5	1.7578243927806573E-5	3.0761926873661507E-4	5.238316690486359E-4
118-119	16.75160767103315	4.921908299785841E-5	1.7578243927806573E-5	3.093770931293957E-4	5.238316690486359E-4
120-121	18.213693930147997	4.921908299785841E-5	1.7578243927806573E-5	3.093770931293957E-4	5.238316690486359E-4
122-123	19.694000039094014	4.921908299785841E-5	1.7578243927806573E-5	3.1113491752217637E-4	5.308629666197585E-4
124-125	21.189606251554792	4.921908299785841E-5	1.7578243927806573E-5	3.1465056630773766E-4	5.396520885836618E-4
126-127	22.692583021694507	4.921908299785841E-5	1.7578243927806573E-5	3.1640839070051834E-4	5.484412105475652E-4
128-129	24.20531044374097	4.921908299785841E-5	1.7578243927806573E-5	3.1640839070051834E-4	5.484412105475652E-4
130-131	25.705775282823396	4.921908299785841E-5	1.7578243927806573E-5	3.1640839070051834E-4	5.501990349403458E-4
132-133	27.193863280356254	4.921908299785841E-5	1.7578243927806573E-5	3.1640839070051834E-4	5.537146837259071E-4
134-135	28.678671177572184	5.4492556176200384E-5	1.7578243927806573E-5	3.1640839070051834E-4	5.572303325114684E-4
136-137	30.13821562261507	5.625038056898104E-5	1.7578243927806573E-5	3.18166215093299E-4	5.835976984031782E-4
138-139	31.57620562495368	5.800820496176169E-5	1.933606832058723E-5	3.216818638788603E-4	5.906289959743008E-4
>>END_MODULE
