##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261626_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29402681
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.338909094718268	32.0	32.0	32.0	32.0	32.0
2	31.65477671917061	32.0	32.0	32.0	32.0	32.0
3	35.672749944129244	37.0	37.0	37.0	32.0	37.0
4	36.30629931331772	37.0	37.0	37.0	37.0	37.0
5	36.49633178008495	37.0	37.0	37.0	37.0	37.0
6	40.084364483633316	41.0	41.0	41.0	37.0	41.0
7	40.22205645124674	41.0	41.0	41.0	37.0	41.0
8	40.24333869418234	41.0	41.0	41.0	41.0	41.0
9	40.26574481422289	41.0	41.0	41.0	41.0	41.0
10-14	40.29494196804707	41.0	41.0	41.0	41.0	41.0
15-19	40.27066518185875	41.0	41.0	41.0	41.0	41.0
20-24	40.25658932938803	41.0	41.0	41.0	41.0	41.0
25-29	40.192002742879126	41.0	41.0	41.0	41.0	41.0
30-34	40.17307757071541	41.0	41.0	41.0	41.0	41.0
35-39	40.157857747733956	41.0	41.0	41.0	41.0	41.0
40-44	40.076900837716124	41.0	41.0	41.0	38.6	41.0
45-49	40.042050124612786	41.0	41.0	41.0	37.0	41.0
50-54	40.07212511675381	41.0	41.0	41.0	37.0	41.0
55-59	40.043937673574725	41.0	41.0	41.0	37.0	41.0
60-64	40.00403340770183	41.0	41.0	41.0	37.0	41.0
65-69	39.96132413231297	41.0	41.0	41.0	37.0	41.0
70-74	39.91765657016107	41.0	41.0	41.0	37.0	41.0
75-79	39.480169049890385	41.0	40.2	41.0	36.0	41.0
80-84	39.83689585313666	41.0	41.0	41.0	37.0	41.0
85-89	39.821578828134754	41.0	41.0	41.0	37.0	41.0
90-94	39.775503383517986	41.0	41.0	41.0	37.0	41.0
95-99	39.72470286638147	41.0	41.0	41.0	37.0	41.0
100-104	39.6656506935541	41.0	41.0	41.0	37.0	41.0
105-109	39.55892887454719	41.0	41.0	41.0	37.0	41.0
110-114	39.46788610195104	41.0	41.0	41.0	37.0	41.0
115-119	39.36779573264084	41.0	41.0	41.0	37.0	41.0
120-124	39.23325951806912	41.0	41.0	41.0	37.0	41.0
125-129	39.07126427688686	41.0	41.0	41.0	37.0	41.0
130-134	38.86674090706218	41.0	41.0	41.0	33.0	41.0
135-139	38.62930087905929	41.0	41.0	41.0	32.0	41.0
140-144	38.368177650194546	41.0	41.0	41.0	32.0	41.0
145-149	38.0849447980611	41.0	39.4	41.0	31.0	41.0
150	34.289254915223545	37.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	18.0
13	31.0
14	50.0
15	41.0
16	70.0
17	124.0
18	423.0
19	1229.0
20	2746.0
21	5610.0
22	10784.0
23	19643.0
24	34496.0
25	62006.0
26	135785.0
27	118758.0
28	117902.0
29	149636.0
30	187069.0
31	230815.0
32	281213.0
33	339928.0
34	414329.0
35	515971.0
36	669398.0
37	921227.0
38	1402815.0
39	2796786.0
40	2.0983778E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.360794573737984	9.374078364143132	10.110177389149909	42.154949672968975
2	24.78362572423097	11.383643962981935	39.47232048444735	24.360409828339748
3	24.947429113692046	14.781502407892669	23.82476278268638	36.4463056957289
4	27.434336850709958	23.5497637723758	24.15993640045128	24.855962976462962
5	28.39179869345928	26.791148739123482	26.706353750530436	18.1106988168868
6	22.328331975180493	27.648047728982526	29.473503775846282	20.550116519990695
7	19.365741091564686	21.721612952509794	41.9244183428405	16.98822761308502
8	20.353100480444148	21.161615661756112	35.307708080992256	23.177575776807487
9	19.720630492115074	21.754355999676626	36.661364055082	21.863649453126303
10-14	24.76156680016667	25.083941058020464	27.659792541174205	22.49469960063866
15-19	25.1036867248893	24.125028845184218	27.482701817233853	23.28858261269263
20-24	24.696255587518607	24.604538626430557	27.481762057527952	23.217443728522884
25-29	24.55692843421619	24.57544388072761	27.478691345907386	23.38893633914881
30-34	24.444482642509684	24.704087471568045	26.891415405968395	23.960014479953877
35-39	24.75165614833057	24.652430575532946	27.05194760899552	23.543965667140963
40-44	24.4794606149127	24.455276470330496	27.605431421356684	23.45983149340012
45-49	24.53264656403896	24.35658686922662	27.566559772413136	23.54420679432129
50-54	24.545280855318104	24.676276907451623	27.065186296547743	23.71325594068253
55-59	24.712612192169956	24.568056098302524	26.950502229218483	23.768829480309037
60-64	24.42336410886498	24.615488720739684	27.00581590457852	23.955331265816817
65-69	24.61982124061283	25.44318870921341	26.66361990864229	23.27337014153147
70-74	24.628421057779708	25.247790206922758	26.608228649317905	23.515560085979622
75-79	24.725292848153277	25.113610066359016	26.43582749389488	23.725269591592827
80-84	24.566723422887137	25.184765632036083	26.569333802707224	23.679177142369557
85-89	24.97295480264873	24.87865321712586	26.537838441468526	23.610553538756886
90-94	25.15590512420584	25.219881462055323	26.116556911351136	23.5076565023877
95-99	25.376520658185502	25.29577505487353	25.96511450367867	23.362589783262298
100-104	25.22306477202603	25.544470033476646	25.622146151762593	23.61031904273473
105-109	25.44859996672252	25.751896301865894	25.10813531983614	23.691368411575446
110-114	25.75204404481007	26.174071551559212	24.65578334715048	23.418101056480243
115-119	25.44989960050455	26.576899165149054	24.22775551110588	23.74544572324052
120-124	25.492444577380503	26.623561881603088	23.89905009113377	23.984943449882643
125-129	25.047119559565296	26.709791679322976	23.877634827445362	24.365453933666366
130-134	24.777909437889623	26.591255809868425	23.97679444161031	24.654040310631643
135-139	24.477082718751934	26.671414486538637	23.9371115689102	24.914391225799235
140-144	24.21135690945024	26.6099478764475	24.223065565624445	24.955629648477817
145-149	23.824182489378998	26.290266045034016	24.8014833497727	25.08406811581429
150	23.45307038178392	25.99687259790171	25.17236269524964	25.37769432506473
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24.0
1	19.0
2	12.5
3	14.0
4	19.0
5	28.0
6	34.0
7	38.5
8	43.0
9	47.0
10	59.0
11	66.0
12	75.5
13	82.0
14	107.5
15	155.0
16	206.5
17	310.0
18	509.5
19	789.0
20	1403.0
21	2527.0
22	3923.0
23	6017.5
24	9830.5
25	15926.0
26	23890.0
27	34447.0
28	52050.0
29	78290.0
30	108278.5
31	140862.0
32	187821.5
33	251005.0
34	325658.0
35	402248.5
36	474758.0
37	598265.0
38	694162.0
39	685425.0
40	678973.0
41	759904.0
42	847327.0
43	950773.0
44	1074809.5
45	1191161.0
46	1259243.0
47	1289721.0
48	1335106.5
49	1399387.0
50	1499050.0
51	1481390.0
52	1416318.5
53	1394219.0
54	1293100.0
55	1181592.5
56	1076075.0
57	947699.5
58	856673.5
59	752907.5
60	620433.0
61	491141.5
62	384100.0
63	291510.5
64	220375.0
65	167524.0
66	121380.0
67	90532.5
68	67470.5
69	49124.0
70	34826.0
71	22962.5
72	17516.0
73	12724.0
74	8712.0
75	6081.0
76	4294.5
77	2725.0
78	1621.0
79	1035.0
80	665.0
81	435.0
82	293.5
83	159.5
84	74.5
85	33.0
86	19.0
87	8.5
88	6.5
89	4.5
90	4.0
91	5.0
92	4.5
93	6.0
94	5.0
95	3.5
96	2.5
97	1.0
98	2.0
99	3.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.032582709039356
2	0.012713126398235589
3	0.0
4	0.021242960803472308
5	0.0
6	3.4010504008120894E-6
7	0.003390847249609653
8	0.0017413378052157898
9	6.393974753526728E-4
10-14	2.8976949414919E-4
15-19	8.842731042111432E-5
20-24	1.6869209988027963E-4
25-29	0.04845068379988886
30-34	0.0012835564212664824
35-39	0.004725419426888316
40-44	0.03919234439879819
45-49	0.10831461253482293
50-54	9.121617174978024E-4
55-59	0.002746008093615681
60-64	0.001746779485857089
65-69	0.0016978043600853947
70-74	2.9385075463016447E-4
75-79	7.645561301025577E-4
80-84	0.0022997902810291348
85-89	0.0014223192776196157
90-94	8.427802893212356E-4
95-99	0.0024099843140154465
100-104	0.0037710846844204444
105-109	7.332664664150865E-4
110-114	0.002741246623054544
115-119	1.4692537731508223E-4
120-124	0.00138694835345117
125-129	0.0011822051193222823
130-134	1.2175760434907279E-4
135-139	0.0018862225522903845
140-144	0.0017086877213679935
145-149	0.01407830802912156
150	6.802100801624179E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.9402681E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.719531592443307
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.85927672204947	19.641859597145327
2	13.138298701736273	7.283749718674709
3	5.024380880381461	4.17820453638606
4	2.684918933150681	2.9769878076247784
5	1.6807204161297977	2.3294391336487195
6	1.1147445284012703	1.8540117703513395
7	0.7832619594028374	1.51981582401782
8	0.5960980047426151	1.321884597972427
9	0.4636900978699305	1.15679450853078
>10	3.0448791850103736	16.264063320511653
>50	0.3147827506182048	6.098490230602496
>100	0.24459751450502165	13.554006978165281
>500	0.028515882617575733	5.489797974964274
>1k	0.019531331495816293	10.533650674177274
>5k	0.0017912936910842454	3.404432735498783
>10k+	5.117981974515578E-4	2.392810591728436
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG	127791	0.4346236317701777	TruSeq Adapter, Index 7 (97% over 34bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0883361282598685E-4	3.401050400812089E-6	0.0	3.401050400812089E-6	6.802100801624178E-6
2	1.9726092324710118E-4	3.401050400812089E-6	0.0	1.7005252004060446E-5	1.3604201603248356E-5
3	2.6188088086253085E-4	3.401050400812089E-6	0.0	1.7005252004060446E-5	3.06094536073088E-5
4	3.9452184649420237E-4	3.401050400812089E-6	0.0	3.06094536073088E-5	3.741155440893298E-5
5	5.033554593201892E-4	3.401050400812089E-6	0.0	3.06094536073088E-5	4.0812604809745073E-5
6	6.053869713445519E-4	3.401050400812089E-6	0.0	3.06094536073088E-5	4.421365521055716E-5
7	6.359964249518607E-4	3.401050400812089E-6	0.0	3.06094536073088E-5	4.421365521055716E-5
8	6.87012180964042E-4	3.401050400812089E-6	0.0	3.741155440893298E-5	5.101575601218134E-5
9	9.795025154338817E-4	3.401050400812089E-6	0.0	4.761470561136925E-5	5.4416806412993424E-5
10-11	0.001256688123100067	3.401050400812089E-6	0.0	4.931523081177529E-5	6.12189072146176E-5
12-13	0.001571285285175185	6.802100801624178E-6	0.0	5.101575601218134E-5	7.99246844190841E-5
14-15	0.0017651451580214745	6.802100801624178E-6	0.0	5.2716281212587384E-5	9.52294112227385E-5
16-17	0.001873978770847461	6.802100801624178E-6	0.0	5.611733161339947E-5	9.863046162355059E-5
18-19	0.0019981171104771025	6.802100801624178E-6	0.0	5.781785681380552E-5	9.863046162355059E-5
20-21	0.002101849147701871	6.802100801624178E-6	0.0	6.291943241502365E-5	1.0883361282598685E-4
22-23	0.002203880659726234	6.802100801624178E-6	0.0	6.802100801624178E-5	1.1223466322679894E-4
24-25	0.0023008105961493785	6.802100801624178E-6	0.0	9.182836082192642E-5	1.1223466322679894E-4
26-27	0.0023688316041656204	6.802100801624178E-6	0.0	1.0373203722476872E-4	1.1563571362761102E-4
28-29	0.002521878872202164	6.802100801624178E-6	0.0	1.0713308762558082E-4	1.1903676402842312E-4
30-31	0.00275144977425698	6.802100801624178E-6	0.0	1.0883361282598685E-4	1.1903676402842312E-4
32-33	0.0030932553395385948	6.802100801624178E-6	0.0	1.0883361282598685E-4	1.292399152308594E-4
34-35	0.0035966107988587843	6.802100801624178E-6	0.0	1.0883361282598685E-4	1.3094044043126542E-4
36-37	0.0043686492398431285	1.0203151202436268E-5	0.0	1.105341380263929E-4	1.445446420345138E-4
38-39	0.005213810264444932	1.0203151202436268E-5	0.0	1.1223466322679894E-4	1.751540956418226E-4
40-41	0.006383771602324291	1.0203151202436268E-5	0.0	1.1903676402842312E-4	1.90458822445477E-4
42-43	0.007905741656687702	1.0203151202436268E-5	0.0	1.1903676402842312E-4	1.938598728462891E-4
44-45	0.009847741435551404	1.3604201603248356E-5	0.0	1.2073728922882917E-4	1.989614484475072E-4
46-47	0.01231350297614017	1.3604201603248356E-5	0.0	1.224378144292352E-4	2.0406302404872535E-4
48-49	0.015309828379255621	1.3604201603248356E-5	0.0	1.2753939003045336E-4	2.091645996499435E-4
50-51	0.019127507454167192	1.3604201603248356E-5	0.0	1.292399152308594E-4	2.2616985165400393E-4
52-53	0.024053928959743502	1.3604201603248356E-5	0.0	1.292399152308594E-4	2.3807352805684624E-4
54-55	0.03013840812679633	1.7005252004060446E-5	0.0	1.3604201603248356E-4	2.567793052613127E-4
56-57	0.03770404474340282	2.8908928406902756E-5	0.0	1.4284411683410775E-4	2.6528193126334294E-4
58-59	0.04693619605640724	3.06094536073088E-5	0.0	1.445446420345138E-4	2.6528193126334294E-4
60-61	0.05860690050679392	3.06094536073088E-5	0.0	1.479456924353259E-4	2.66982456463749E-4
62-63	0.07427383917813482	3.401050400812089E-5	0.0	1.4964621763573192E-4	2.6868298166415503E-4
64-65	0.09479917834703577	3.401050400812089E-5	0.0	1.53047268036544E-4	2.7208403206496713E-4
66-67	0.12203989153234021	3.401050400812089E-5	0.0	1.53047268036544E-4	2.8738875886862155E-4
68-69	0.15766589448084684	3.401050400812089E-5	0.0	1.53047268036544E-4	3.1799821247593035E-4
70-71	0.2040205109187152	3.401050400812089E-5	0.0	1.53047268036544E-4	3.265008384779606E-4
72-73	0.26595874029310457	3.401050400812089E-5	0.0	1.5814884363776215E-4	3.3500346447999077E-4
74-75	0.3479750707086881	3.401050400812089E-5	0.0	1.632504192389803E-4	3.43506090482021E-4
76-77	0.4540419290336143	3.401050400812089E-5	0.0	1.632504192389803E-4	3.571102920852694E-4
78-79	0.5880059032712017	3.401050400812089E-5	1.7005252004060445E-6	1.632504192389803E-4	3.605113424860815E-4
80-81	0.7564038122918111	3.401050400812089E-5	3.401050400812089E-6	1.6665146963979236E-4	3.605113424860815E-4
82-83	0.9683997864004306	3.401050400812089E-5	3.401050400812089E-6	1.7005252004060446E-4	3.605113424860815E-4
84-85	1.23224307334423	3.401050400812089E-5	3.401050400812089E-6	1.8195619644344679E-4	3.605113424860815E-4
86-87	1.5628404770299689	3.401050400812089E-5	3.401050400812089E-6	1.8875829724507095E-4	3.639123928868935E-4
88-89	1.958525482761249	3.401050400812089E-5	3.401050400812089E-6	1.9556039804669514E-4	3.673134432877056E-4
90-91	2.4237670707647374	3.571102920852694E-5	3.401050400812089E-6	2.0406302404872535E-4	3.673134432877056E-4
92-93	2.973055416273094	3.741155440893298E-5	3.401050400812089E-6	2.0406302404872535E-4	3.775165944901419E-4
94-95	3.6109921404786185	3.9112079609339026E-5	3.401050400812089E-6	2.057635492491314E-4	3.8942027089298423E-4
96-97	4.336873225948342	4.0812604809745073E-5	6.802100801624178E-6	2.1086512485034954E-4	3.911207960933903E-4
98-99	5.155023108266896	4.421365521055716E-5	6.802100801624178E-6	2.1086512485034954E-4	3.911207960933903E-4
100-101	6.069126825543561	4.421365521055716E-5	1.0203151202436268E-5	2.1596670045156768E-4	3.928213212937963E-4
102-103	7.070635837595898	4.421365521055716E-5	1.0203151202436268E-5	2.1936775085237974E-4	3.9452184649420237E-4
104-105	8.177451573208579	4.421365521055716E-5	1.0203151202436268E-5	2.3127142725522208E-4	3.9452184649420237E-4
106-107	9.373859479004652	4.421365521055716E-5	1.0203151202436268E-5	2.3807352805684624E-4	3.9452184649420237E-4
108-109	10.656989068445833	4.761470561136925E-5	1.0203151202436268E-5	2.4317510365806438E-4	3.9452184649420237E-4
110-111	11.999353052192758	4.761470561136925E-5	1.0203151202436268E-5	2.465761540588765E-4	3.9452184649420237E-4
112-113	13.405396603119286	4.761470561136925E-5	1.0203151202436268E-5	2.482766792592825E-4	3.9792289689501446E-4
114-115	14.870033450351007	4.761470561136925E-5	1.3604201603248356E-5	2.482766792592825E-4	4.013239472958265E-4
116-117	16.3843987560182	4.761470561136925E-5	1.3604201603248356E-5	2.4997720445968857E-4	4.047249976966386E-4
118-119	17.94749431182823	4.761470561136925E-5	1.3604201603248356E-5	2.516777296600946E-4	4.081260480974507E-4
120-121	19.51904487893468	4.761470561136925E-5	1.3604201603248356E-5	2.516777296600946E-4	4.0982657329785674E-4
122-123	21.102806917505244	4.761470561136925E-5	1.3604201603248356E-5	2.516777296600946E-4	4.1322762369866884E-4
124-125	22.699326296129254	4.761470561136925E-5	1.3604201603248356E-5	2.516777296600946E-4	4.2513130010151117E-4
126-127	24.30043879331956	4.761470561136925E-5	1.3604201603248356E-5	2.5337825486050066E-4	4.4043602690516554E-4
128-129	25.912351326057646	4.761470561136925E-5	1.3604201603248356E-5	2.550787800609067E-4	4.421365521055716E-4
130-131	27.516353015563446	4.761470561136925E-5	1.3604201603248356E-5	2.550787800609067E-4	4.4383707730597763E-4
132-133	29.10909212666695	5.101575601218134E-5	1.3604201603248356E-5	2.550787800609067E-4	4.489386529071958E-4
134-135	30.67695935618932	6.12189072146176E-5	1.3604201603248356E-5	2.550787800609067E-4	4.540402285084139E-4
136-137	32.21367636509065	6.12189072146176E-5	1.3604201603248356E-5	2.601803556621248E-4	4.693449553120683E-4
138-139	33.72990884742789	6.291943241502365E-5	1.53047268036544E-5	2.635814060629369E-4	4.7104548051247433E-4
>>END_MODULE
