##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261625_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29185546
Sequences flagged as poor quality	0
Sequence length	150
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.319582542673693	32.0	32.0	32.0	32.0	32.0
2	31.670877666636766	32.0	32.0	32.0	32.0	32.0
3	35.699879214183625	37.0	37.0	37.0	32.0	37.0
4	36.301226538643476	37.0	37.0	37.0	37.0	37.0
5	36.49191630679104	37.0	37.0	37.0	37.0	37.0
6	40.04189731451315	41.0	41.0	41.0	37.0	41.0
7	40.113048870149626	41.0	41.0	41.0	37.0	41.0
8	40.140501979986944	41.0	41.0	41.0	37.0	41.0
9	40.18987446731337	41.0	41.0	41.0	37.0	41.0
10-14	40.25899309199149	41.0	41.0	41.0	41.0	41.0
15-19	40.2492480079009	41.0	41.0	41.0	41.0	41.0
20-24	40.24365490369787	41.0	41.0	41.0	41.0	41.0
25-29	40.18094626703232	41.0	41.0	41.0	41.0	41.0
30-34	40.163906901039304	41.0	41.0	41.0	41.0	41.0
35-39	40.15142938905443	41.0	41.0	41.0	41.0	41.0
40-44	40.055878207658	41.0	41.0	41.0	37.8	41.0
45-49	40.033370066127944	41.0	41.0	41.0	37.0	41.0
50-54	40.061503224918255	41.0	41.0	41.0	37.0	41.0
55-59	40.032749526083904	41.0	41.0	41.0	37.0	41.0
60-64	39.994391093454276	41.0	41.0	41.0	37.0	41.0
65-69	39.95691607071527	41.0	41.0	41.0	37.0	41.0
70-74	39.9121220003902	41.0	41.0	41.0	37.0	41.0
75-79	39.43125445725771	41.0	40.2	41.0	36.0	41.0
80-84	39.78209091582526	41.0	41.0	41.0	37.0	41.0
85-89	39.76851048118134	41.0	41.0	41.0	37.0	41.0
90-94	39.72243999821008	41.0	41.0	41.0	37.0	41.0
95-99	39.672774735823	41.0	41.0	41.0	37.0	41.0
100-104	39.611843156883204	41.0	41.0	41.0	37.0	41.0
105-109	39.506813742665635	41.0	41.0	41.0	37.0	41.0
110-114	39.4179033964278	41.0	41.0	41.0	37.0	41.0
115-119	39.31970397949725	41.0	41.0	41.0	37.0	41.0
120-124	39.184848952286174	41.0	41.0	41.0	37.0	41.0
125-129	39.02143894104294	41.0	41.0	41.0	37.0	41.0
130-134	38.81680764170045	41.0	41.0	41.0	32.0	41.0
135-139	38.58131995885909	41.0	41.0	41.0	32.0	41.0
140-144	38.326084555690684	41.0	41.0	41.0	32.0	41.0
145-149	38.040321692114304	41.0	39.4	41.0	31.0	41.0
150	34.25493235589973	37.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	7.0
13	10.0
14	32.0
15	47.0
16	58.0
17	151.0
18	376.0
19	1061.0
20	2734.0
21	5688.0
22	11083.0
23	20640.0
24	37043.0
25	69779.0
26	164696.0
27	128798.0
28	115762.0
29	147134.0
30	184975.0
31	228006.0
32	278840.0
33	337499.0
34	415015.0
35	517640.0
36	671043.0
37	924920.0
38	1412971.0
39	2811957.0
40	2.0697581E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.082650049468945	9.176783588019076	9.634907414103168	41.105658948408816
2	25.275168796707092	11.040704308207557	39.094226484911225	24.589900410174124
3	25.4859477359101	14.485982205027103	23.346371522396737	36.68169853666606
4	28.195760887665177	23.314449569651767	23.472243979136074	25.01754556354698
5	28.919128593251507	26.623889552992395	26.037119609625915	18.419862244130176
6	22.53246864047018	27.919141893045275	28.61414687941764	20.9342425870669
7	19.501918222868834	21.67203794197378	41.46221127503528	17.363832560122106
8	21.136033742777247	20.454036865679534	34.74632252403638	23.66360686750684
9	20.335330648675264	21.40949654078076	35.68723862522363	22.56793418532035
10-14	25.278460229464123	24.767493474250625	26.858582638634683	23.095463657650576
15-19	25.55315463124024	23.58337365869697	27.04713046219942	23.816341247863367
20-24	25.158982052715253	24.150647625116907	27.084108861252297	23.606261460915537
25-29	24.996985096304353	24.189293253234457	26.90693395764156	23.90678769281963
30-34	24.90804680990167	24.356747060312358	26.330769104572394	24.404437025213582
35-39	25.120822017139478	24.250234775936537	26.59544481743535	24.033498389488646
40-44	24.924416418160483	23.97539919204621	27.143550970526842	23.95663341926646
45-49	25.043510902267013	23.96903668772875	26.95127746198031	24.036174948023927
50-54	25.067951210169365	24.218894384650486	26.568325443362394	24.14482896181776
55-59	24.98983578822256	24.04310579716422	26.719585624712522	24.247472789900698
60-64	24.875889679187928	24.0577233216031	26.701320627847906	24.36506637136106
65-69	24.957855146865153	25.161167400644985	26.118300314931016	23.762677137558853
70-74	25.005855484644822	24.973257743450574	26.1080681078095	23.912818664095102
75-79	25.076020033240532	24.765274908699688	26.08036765535368	24.078337402706097
80-84	25.000371767678764	24.780648503452465	26.16314799466184	24.05583173420693
85-89	25.367474181837746	24.625888881772635	26.092037127755415	23.9145998086342
90-94	25.37491006429101	24.78709893224028	25.803326530272123	24.03466447319658
95-99	25.58184468970275	24.956626837075348	25.658485093260936	23.803043379960968
100-104	25.602160389374685	25.20895874486433	25.252542814071877	23.936338051689106
105-109	25.711060082282565	25.409321530226453	24.7939000702017	24.08571831728928
110-114	25.910761170036295	25.75792146443121	24.45675414475508	23.874563220777418
115-119	25.735558249556362	26.16653518131178	24.007590973305845	24.090315595826013
120-124	25.693059931486808	26.259067590011043	23.69831759112805	24.349554887374104
125-129	25.33165817461558	26.413706754104183	23.64035589445349	24.61427917682675
130-134	25.0782246069003	26.340340662342527	23.707916504619927	24.873518226137247
135-139	24.780835396676036	26.393408028495507	23.711055205994004	25.114701368834453
140-144	24.493632708553317	26.339128592211086	24.012996517833898	25.1542421814017
145-149	24.131111085280374	26.14163978153119	24.44793039057772	25.279318742610712
150	23.78235951332619	25.920441983195513	24.78207361836394	25.515124885114354
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	14.0
2	9.0
3	12.5
4	18.0
5	23.0
6	23.0
7	21.0
8	23.5
9	35.5
10	44.0
11	57.5
12	57.0
13	74.5
14	111.0
15	137.0
16	196.5
17	320.0
18	540.0
19	793.0
20	1458.0
21	2631.5
22	3884.5
23	5798.0
24	9156.5
25	14421.0
26	22083.0
27	32462.5
28	48567.0
29	72299.0
30	99562.5
31	127986.5
32	168654.5
33	222164.0
34	279506.0
35	339154.5
36	408206.5
37	499564.0
38	569526.0
39	595887.5
40	624781.0
41	696341.5
42	767201.0
43	854037.0
44	970795.5
45	1092307.5
46	1160683.5
47	1191287.0
48	1246056.5
49	1325602.0
50	1440922.0
51	1463656.5
52	1435740.5
53	1443837.0
54	1372703.5
55	1266935.0
56	1167137.0
57	1055885.5
58	974982.0
59	873106.5
60	730226.5
61	588292.5
62	472539.0
63	367381.0
64	280763.0
65	214888.5
66	156189.0
67	118181.5
68	88986.5
69	63991.0
70	45964.5
71	31111.0
72	23850.0
73	17590.0
74	11966.0
75	8393.5
76	6005.0
77	3821.0
78	2197.0
79	1366.5
80	896.5
81	595.0
82	386.5
83	214.0
84	106.5
85	58.5
86	30.0
87	14.0
88	7.5
89	5.5
90	4.0
91	4.0
92	3.0
93	4.0
94	3.5
95	0.5
96	1.0
97	2.5
98	2.5
99	3.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0201042666805
2	0.012160128852823244
3	0.0
4	0.019889982527652558
5	6.852707158536626E-6
6	0.0
7	0.003361252861262215
8	0.001764572093323181
9	6.955497765914675E-4
10-14	2.932958663853676E-4
15-19	9.251154664024446E-5
20-24	1.72688220395123E-4
25-29	0.047289161559629554
30-34	0.0012691213657609831
35-39	0.004581034735481734
40-44	0.038219603635306326
45-49	0.10547686858419575
50-54	9.16206947096347E-4
55-59	0.002720524741939041
60-64	0.0017248263918036688
65-69	0.0016706900052512296
70-74	3.0220438569146524E-4
75-79	7.291280416682971E-4
80-84	0.002236723616546355
85-89	0.0013486127688000081
90-94	8.67552726270737E-4
95-99	0.0023258088096073312
100-104	0.003526403103782948
105-109	7.31183853815858E-4
110-114	0.002471771472084161
115-119	1.356836017390252E-4
120-124	0.0013472422273683009
125-129	0.001135493576169519
130-134	1.2540454100122024E-4
135-139	0.001854342557100011
140-144	0.0014945754312768382
145-149	0.014106982956563497
150	6.852707158536626E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.9185546E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.121295298353946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.24830786517018	21.46091320714825
2	12.959242516343384	7.80698341355525
3	5.116190433510281	4.6231884855113
4	2.622802798757231	3.1600887044286305
5	1.5843526261262884	2.386137665413625
6	1.102534543045097	1.992586112861826
7	0.8030544084450981	1.6932327284193822
8	0.5970143550342515	1.4386276548274413
9	0.45308863045400916	1.2282854790808777
>10	2.943583010401464	16.953278061777016
>50	0.29999202226407573	6.317446823348735
>100	0.22824169274025585	13.69156175763723
>500	0.02467034936498581	5.093997181908206
>1k	0.015644018645168116	8.665583210403987
>5k	0.0011147091817856568	2.2007992481053176
>10k+	1.660205164337083E-4	1.287290265572859
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG	181889	0.6232160261795342	TruSeq Adapter, Index 7 (97% over 34bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8502309328048892E-4	0.0	0.0	3.426353579268313E-6	3.426353579268313E-6
2	2.29565689810977E-4	6.852707158536626E-6	0.0	1.3705414317073253E-5	3.768988937195144E-5
3	2.9466640781707494E-4	6.852707158536626E-6	0.0	1.3705414317073253E-5	9.936425379878108E-5
4	4.59131379621954E-4	1.027906073780494E-5	0.0	1.7131767896341565E-5	1.1306966811585433E-4
5	5.893328156341499E-4	1.027906073780494E-5	0.0	2.055812147560988E-5	1.1649602169512264E-4
6	7.675032017561022E-4	1.027906073780494E-5	0.0	2.055812147560988E-5	1.1992237527439096E-4
7	8.188985054451268E-4	1.027906073780494E-5	0.0	2.3984475054878193E-5	1.1992237527439096E-4
8	8.771465162926882E-4	1.027906073780494E-5	0.0	3.426353579268313E-5	1.267750824329276E-4
9	0.0011581075097926898	1.027906073780494E-5	0.0	4.111624295121976E-5	1.302014360121959E-4
10-11	0.00142193673539635	1.7131767896341565E-5	0.0	4.111624295121976E-5	1.336277895914642E-4
12-13	0.0017166031432134248	1.7131767896341565E-5	0.0	4.111624295121976E-5	1.4562002711890332E-4
14-15	0.0018964867061250115	1.7131767896341565E-5	0.0	4.111624295121976E-5	1.541859110670741E-4
16-17	0.002026688142137207	1.8844944685975724E-5	0.0	4.282941974085392E-5	1.541859110670741E-4
18-19	0.002165455462097574	2.3984475054878193E-5	0.0	4.7968950109756386E-5	1.5589908785670824E-4
20-21	0.0022613933623170865	2.7410828634146505E-5	0.0	4.7968950109756386E-5	1.576122646463424E-4
22-23	0.0023641839696951364	3.083718221341482E-5	0.0	6.167436442682963E-5	1.576122646463424E-4
24-25	0.0024909590521280636	3.083718221341482E-5	0.0	7.88061323231712E-5	1.6617814859451317E-4
26-27	0.0025971760130853814	3.2550359003048973E-5	3.426353579268313E-6	8.565883948170783E-5	1.7474403254268397E-4
28-29	0.0027993308742622117	3.426353579268313E-5	3.426353579268313E-6	8.7372016271342E-5	1.815967397012206E-4
30-31	0.0030134779729664813	3.426353579268313E-5	3.426353579268313E-6	9.593790021951277E-5	1.8502309328048892E-4
32-33	0.0034383458167957523	3.5976712582317286E-5	3.426353579268313E-6	9.936425379878108E-5	1.9701533080792802E-4
34-35	0.004000267803795756	3.768988937195144E-5	3.426353579268313E-6	9.936425379878108E-5	2.1072074512500127E-4
36-37	0.0047129493482835644	3.768988937195144E-5	3.426353579268313E-6	1.027906073780494E-4	2.3984475054878194E-4
38-39	0.005728863184536619	3.768988937195144E-5	3.426353579268313E-6	1.0964331453658602E-4	3.0151911497561154E-4
40-41	0.006933226467649432	3.768988937195144E-5	3.426353579268313E-6	1.1135649132622017E-4	3.237904132408556E-4
42-43	0.008516201821271392	3.94030661615856E-5	3.426353579268313E-6	1.1649602169512264E-4	3.546275954542704E-4
44-45	0.010498347366878111	4.454259653048807E-5	3.426353579268313E-6	1.1992237527439096E-4	3.7347254014024613E-4
46-47	0.012972174651109834	4.7968950109756386E-5	3.426353579268313E-6	1.1992237527439096E-4	3.8717795445731944E-4
48-49	0.015999358038393387	4.968212689939054E-5	3.426353579268313E-6	1.2334872885365927E-4	3.9574383840549016E-4
50-51	0.01976320744521963	5.13953036890247E-5	3.426353579268313E-6	1.267750824329276E-4	4.180151366707342E-4
52-53	0.02418491673926539	5.3108480478658854E-5	3.426353579268313E-6	1.267750824329276E-4	4.300073741981733E-4
54-55	0.03010051619387213	6.167436442682963E-5	3.426353579268313E-6	1.267750824329276E-4	4.317205509878075E-4
56-57	0.03753570346088437	7.024024837500042E-5	3.426353579268313E-6	1.267750824329276E-4	4.317205509878075E-4
58-59	0.047018136991509425	7.195342516463457E-5	3.426353579268313E-6	1.267750824329276E-4	4.334337277774416E-4
60-61	0.058880173082936325	7.195342516463457E-5	3.426353579268313E-6	1.302014360121959E-4	4.3686008135670996E-4
62-63	0.07454203529377179	7.195342516463457E-5	3.426353579268313E-6	1.302014360121959E-4	4.4371278851524656E-4
64-65	0.09377587111099446	7.195342516463457E-5	3.426353579268313E-6	1.302014360121959E-4	4.4885231888414903E-4
66-67	0.11994807292623547	7.195342516463457E-5	3.426353579268313E-6	1.302014360121959E-4	4.4885231888414903E-4
68-69	0.15483691824713508	7.195342516463457E-5	3.426353579268313E-6	1.302014360121959E-4	4.745499707286614E-4
70-71	0.1998026694446628	7.195342516463457E-5	3.426353579268313E-6	1.302014360121959E-4	4.865422082561005E-4
72-73	0.2595257255080991	7.195342516463457E-5	3.426353579268313E-6	1.336277895914642E-4	4.865422082561005E-4
74-75	0.3374238741327642	7.195342516463457E-5	3.426353579268313E-6	1.3705414317073252E-4	4.899685618353688E-4
76-77	0.4386589169858258	7.195342516463457E-5	3.426353579268313E-6	1.4048049675000084E-4	5.002476225731737E-4
78-79	0.5681596636910613	7.195342516463457E-5	3.426353579268313E-6	1.4562002711890332E-4	5.053871529420763E-4
80-81	0.7285866778027726	7.537977874390288E-5	6.852707158536626E-6	1.4904638069817162E-4	5.13953036890247E-4
82-83	0.9317231892800635	7.537977874390288E-5	6.852707158536626E-6	1.5075955748780577E-4	5.173793904695153E-4
84-85	1.1866558878151534	7.537977874390288E-5	6.852707158536626E-6	1.5589908785670824E-4	5.173793904695153E-4
86-87	1.499975364517765	7.537977874390288E-5	6.852707158536626E-6	1.7131767896341564E-4	5.242320976280518E-4
88-89	1.8766018631277277	7.537977874390288E-5	6.852707158536626E-6	1.781703861219523E-4	5.293716279969544E-4
90-91	2.3212740306451694	8.223248590243952E-5	6.852707158536626E-6	1.815967397012206E-4	5.310848047865885E-4
92-93	2.8430151692210934	8.908519306097615E-5	6.852707158536626E-6	1.815967397012206E-4	5.413638655243935E-4
94-95	3.453476594201801	8.908519306097615E-5	6.852707158536626E-6	1.850230932804889E-4	5.58495633420735E-4
96-97	4.148356518668521	9.251154664024446E-5	6.852707158536626E-6	2.0215486117683047E-4	5.670615173689057E-4
98-99	4.931537001226566	9.251154664024446E-5	6.852707158536626E-6	2.055812147560988E-4	5.6877469415854E-4
100-101	5.805414776204632	9.251154664024446E-5	6.852707158536626E-6	2.090075683353671E-4	5.722010477378083E-4
102-103	6.764225003705601	9.251154664024446E-5	6.852707158536626E-6	2.090075683353671E-4	5.722010477378083E-4
104-105	7.822456019839409	9.251154664024446E-5	6.852707158536626E-6	2.1243392191463542E-4	5.722010477378083E-4
106-107	8.964879396122999	9.251154664024446E-5	6.852707158536626E-6	2.1243392191463542E-4	5.739142245274424E-4
108-109	10.19546970270832	9.251154664024446E-5	6.852707158536626E-6	2.1586027549390374E-4	5.756274013170766E-4
110-111	11.479761248941514	9.422472342987862E-5	6.852707158536626E-6	2.1928662907317204E-4	5.807669316859792E-4
112-113	12.824699253527758	9.593790021951277E-5	6.852707158536626E-6	2.1928662907317204E-4	5.876196388445156E-4
114-115	14.230295708704576	9.593790021951277E-5	6.852707158536626E-6	2.2271298265244037E-4	5.961855227926865E-4
116-117	15.685536600891414	9.593790021951277E-5	6.852707158536626E-6	2.2271298265244037E-4	5.996118763719548E-4
118-119	17.179759117749587	9.593790021951277E-5	8.565883948170784E-6	2.2271298265244037E-4	6.01325053161589E-4
120-121	18.678353661774906	9.593790021951277E-5	1.027906073780494E-5	2.2271298265244037E-4	6.047514067408573E-4
122-123	20.19480978700895	9.593790021951277E-5	1.027906073780494E-5	2.2271298265244037E-4	6.081777603201255E-4
124-125	21.72830688176949	9.593790021951277E-5	1.027906073780494E-5	2.2271298265244037E-4	6.23596351426833E-4
126-127	23.265973848835998	9.593790021951277E-5	1.027906073780494E-5	2.2271298265244037E-4	6.338754121646379E-4
128-129	24.809467330164047	9.593790021951277E-5	1.027906073780494E-5	2.2271298265244037E-4	6.338754121646379E-4
130-131	26.342726293350825	9.593790021951277E-5	1.027906073780494E-5	2.2271298265244037E-4	6.355885889542722E-4
132-133	27.872111763816243	1.027906073780494E-4	1.027906073780494E-5	2.2271298265244037E-4	6.390149425335404E-4
134-135	29.38774727736805	1.1306966811585433E-4	1.027906073780494E-5	2.2613933623170866E-4	6.424412961128086E-4
136-137	30.87393499508284	1.1306966811585433E-4	1.027906073780494E-5	2.2613933623170866E-4	6.56146710429882E-4
138-139	32.34552815972673	1.147828449054885E-4	1.541859110670741E-5	2.2785251302134281E-4	6.715653015365894E-4
>>END_MODULE
