##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261623_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28975762
Sequences flagged as poor quality	0
Sequence length	150
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.875321553234734	32.0	32.0	32.0	32.0	32.0
2	31.366501733414292	32.0	32.0	32.0	32.0	32.0
3	35.06760215658867	37.0	37.0	37.0	32.0	37.0
4	35.69339277427803	37.0	37.0	37.0	32.0	37.0
5	35.88420911933222	37.0	37.0	37.0	37.0	37.0
6	39.23827242230938	41.0	41.0	41.0	37.0	41.0
7	39.372652978030395	41.0	41.0	41.0	37.0	41.0
8	39.40419575505901	41.0	41.0	41.0	37.0	41.0
9	39.441729642864956	41.0	41.0	41.0	37.0	41.0
10-14	39.4595819292	41.0	41.0	41.0	37.0	41.0
15-19	39.41948793615851	41.0	41.0	41.0	37.0	41.0
20-24	39.37786883395853	41.0	41.0	41.0	37.0	41.0
25-29	39.34487350496598	41.0	41.0	41.0	37.0	41.0
30-34	39.32051885986639	41.0	41.0	41.0	37.0	41.0
35-39	39.212982678419294	41.0	41.0	41.0	37.0	41.0
40-44	39.16202500558916	41.0	41.0	41.0	37.0	41.0
45-49	39.09896649482419	41.0	41.0	41.0	37.0	41.0
50-54	39.02245932307147	41.0	41.0	41.0	37.0	41.0
55-59	38.97148921225954	41.0	41.0	41.0	37.0	41.0
60-64	38.921357250242465	41.0	41.0	41.0	36.0	41.0
65-69	38.859700207366416	41.0	41.0	41.0	32.0	41.0
70-74	38.801538244274646	41.0	41.0	41.0	32.0	41.0
75-79	38.32702158445393	41.0	39.4	41.0	31.0	41.0
80-84	38.856639966879904	41.0	41.0	41.0	32.0	41.0
85-89	38.83664424079684	41.0	41.0	41.0	32.0	41.0
90-94	38.67694133462305	41.0	41.0	41.0	32.0	41.0
95-99	38.522378013734375	41.0	41.0	41.0	32.0	41.0
100-104	38.329511375749156	41.0	41.0	41.0	32.0	41.0
105-109	38.13863849378663	41.0	39.4	41.0	32.0	41.0
110-114	37.86800474134209	41.0	37.0	41.0	32.0	41.0
115-119	37.54261724678717	41.0	37.0	41.0	28.0	41.0
120-124	37.25211033276709	41.0	37.0	41.0	27.0	41.0
125-129	36.8466126965013	41.0	37.0	41.0	27.0	41.0
130-134	36.26314545929801	41.0	37.0	41.0	22.0	41.0
135-139	35.95898679040779	41.0	36.0	41.0	22.0	41.0
140-144	35.55394294721223	41.0	32.0	41.0	22.0	41.0
145-149	35.22360926349408	41.0	32.0	41.0	22.0	41.0
150	29.470390424935157	32.0	22.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	27.0
12	135.0
13	681.0
14	5505.0
15	27319.0
16	61574.0
17	81226.0
18	86024.0
19	87815.0
20	93420.0
21	101878.0
22	113097.0
23	127932.0
24	146418.0
25	171791.0
26	212113.0
27	229885.0
28	229243.0
29	260082.0
30	299722.0
31	348906.0
32	413396.0
33	495155.0
34	607529.0
35	765806.0
36	1011288.0
37	1417816.0
38	2245229.0
39	4862097.0
40	1.4472653E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.40233087214369	19.803494874533303	12.881915992616397	38.91225826070661
2	24.774971409230428	25.76515277996717	30.333583772585744	19.12629203821666
3	21.309849962161298	26.123161316560427	25.76433211585021	26.802656605428066
4	23.883669694716918	32.95809157702209	21.18784229409879	21.97039643416221
5	26.32209637834546	34.211110651723324	20.07074395489582	19.396049015035395
6	21.460011992091875	33.53351328603541	21.984508983749937	23.02196573812278
7	21.655192516242312	20.326876534625846	32.63780136404591	25.380129585085935
8	20.936458896271855	23.18843270633709	26.91036952902272	28.96473886836834
9	21.338289567673836	23.994999682838365	28.374301252198304	26.292409497289494
10-14	24.65384654887285	26.458286017736604	23.324388718382483	25.563478715008063
15-19	24.48735808915051	26.495180351081014	23.94187528183038	25.075586277938093
20-24	24.334602280017855	26.683619240345145	24.14778805633872	24.833990423298278
25-29	24.161874953534426	26.532719918402954	24.238951809426265	25.066453318636356
30-34	24.12099893914406	26.56302105156608	24.29881370428883	25.017166305001037
35-39	23.981445326008092	26.712009188835207	24.29048374268666	25.016061742470036
40-44	24.01395236430673	26.490353182543164	24.494801108838907	25.000893344311205
45-49	24.025598256458565	26.41242855278536	24.447183353224606	25.114789837531475
50-54	24.144812191728157	26.44861816790262	24.345200256331616	25.061369384037608
55-59	24.17186301276596	26.342013192278273	24.26833240008889	25.21779139486687
60-64	23.956981487293643	26.406528840759197	24.608028020653503	25.02846165129366
65-69	24.137269168087965	26.420844825759577	24.62267871649308	24.819207289659374
70-74	24.127333641508788	26.765339825283124	24.34209289344697	24.765233639761117
75-79	24.046221295973414	26.860048545832726	24.303444647810657	24.790285510383203
80-84	24.10471068365801	27.0104979768395	24.217811311244457	24.66698002825804
85-89	24.33575175036288	26.976209468676014	23.814964278141662	24.873074502819442
90-94	24.469097107092132	27.1136318215821	23.852709063322063	24.56456200800371
95-99	24.709151630029737	27.302349945662087	23.613492798908084	24.375005625400092
100-104	25.366366993400757	27.540890158031452	23.13448406761229	23.958258780955504
105-109	25.773624427539715	27.845527637382787	22.82504194651284	23.55580598856465
110-114	26.46367234360354	27.965664356226522	22.497374353424178	23.073288946745755
115-119	27.21138770683536	27.97940921027787	22.358018452339554	22.451184630547214
120-124	28.00920405663976	28.027864491298743	22.163065542141776	21.79986590991972
125-129	28.900171764567723	28.179207715094883	21.98232789847626	20.938292621861134
130-134	29.911446120277912	28.091569078182367	21.85285235428932	20.144132447250403
135-139	30.510986686571158	28.081639839940397	22.020921940607764	19.386451532880685
140-144	31.09576686887475	28.023357591079055	21.98615104582927	18.894724494216923
145-149	31.71554349763243	27.701919940341718	22.209704603431042	18.372831958594812
150	31.68172368117578	27.655543585010545	22.3753529373335	18.28737979648018
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	65.0
1	67.0
2	70.5
3	86.5
4	109.0
5	121.0
6	131.0
7	158.5
8	190.5
9	230.0
10	282.5
11	320.0
12	367.0
13	441.0
14	519.5
15	617.5
16	764.0
17	970.5
18	1201.5
19	1598.0
20	2379.5
21	3445.0
22	5202.5
23	7731.5
24	11278.5
25	16372.0
26	24369.0
27	36517.5
28	52244.0
29	77521.5
30	106690.0
31	140890.0
32	191074.0
33	254453.5
34	315594.0
35	368291.0
36	434720.5
37	515424.0
38	564226.5
39	593659.0
40	635190.5
41	703202.0
42	786737.0
43	869455.0
44	947049.5
45	1034122.5
46	1121268.5
47	1172885.5
48	1233579.0
49	1331965.0
50	1436555.5
51	1451989.0
52	1417691.5
53	1393048.0
54	1315079.5
55	1244091.0
56	1172129.0
57	1044469.0
58	926420.0
59	802517.0
60	671888.5
61	552594.5
62	446625.5
63	369506.0
64	287185.0
65	210603.0
66	156679.0
67	119840.0
68	96825.0
69	76659.0
70	56840.5
71	42384.0
72	34561.5
73	25722.5
74	17807.0
75	12712.0
76	8707.0
77	5845.5
78	3840.5
79	2699.5
80	1825.5
81	1224.5
82	878.5
83	587.0
84	396.0
85	289.0
86	220.0
87	171.5
88	135.0
89	116.5
90	95.0
91	77.0
92	66.5
93	55.0
94	42.0
95	35.5
96	31.5
97	29.5
98	31.0
99	32.5
100	54.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.786227399300146
2	0.013801190111928721
3	4.76260123892514E-4
4	4.5210200166608216E-4
5	0.0
6	0.0
7	1.7600917622114648E-4
8	7.005855445665242E-4
9	0.0
10-14	3.9895413276793204E-4
15-19	0.0
20-24	8.28278476334807E-5
25-29	2.8299514608105906E-5
30-34	3.554695127603547E-4
35-39	2.6366864829991353E-4
40-44	0.0012831414062553386
45-49	2.4089099020070638E-4
50-54	0.001668290897751024
55-59	5.011084781825582E-4
60-64	3.6789368990537675E-4
65-69	7.930766410905776E-4
70-74	2.0845008321092646E-4
75-79	3.52018352442293E-5
80-84	0.0017352434079214206
85-89	4.893745331011485E-4
90-94	2.981802514805305E-4
95-99	1.5599244637638866E-4
100-104	1.677263914577984E-4
105-109	5.9014841438855E-4
110-114	1.4494873335859124E-4
115-119	3.7272531435066314E-5
120-124	5.797949334343649E-5
125-129	5.873874861341006E-4
130-134	8.351807969709304E-5
135-139	1.9326497781145497E-4
140-144	0.0
145-149	2.415812222643187E-4
150	3.1060442862555266E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.8975762E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.30888207701196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.89396897002067	23.874315296547785
2	12.17434660574454	7.866790576993424
3	4.533759270986497	4.394420809555873
4	2.3003431758708057	2.972860656234762
5	1.3821849567772169	2.2328425388567488
6	0.946404648180209	1.8346365705114216
7	0.6718608344709526	1.51949507311594
8	0.5039502227735635	1.3025654656219976
9	0.4009358118816934	1.1658409079883014
>10	2.6515252845057424	16.441921476878406
>50	0.2809003825332134	6.32423323543959
>100	0.21880312058380783	14.278974948581617
>500	0.025655729949515906	5.7316902467845035
>1k	0.014862097173765684	8.551466287449273
>5k	4.323700746753634E-4	0.9195765358085947
>10k+	6.651847302594136E-5	0.5883693736318072
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC	93160	0.32151009523062757	Illumina Single End PCR Primer 1 (96% over 33bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.176740477092544E-5	0.0	0.0	0.0	0.0
2	6.902320636123392E-5	0.0	0.0	6.902320636123391E-6	0.0
3	7.937668731541901E-5	0.0	0.0	1.0353480954185087E-5	0.0
4	1.656556952669614E-4	0.0	0.0	1.3804641272246783E-5	0.0
5	2.519347032185038E-4	0.0	0.0	1.3804641272246783E-5	0.0
6	3.623718333964781E-4	0.0	0.0	1.725580159030848E-5	0.0
7	4.003345968951567E-4	0.0	0.0	1.725580159030848E-5	3.4511603180616957E-6
8	4.175903984854652E-4	0.0	0.0	1.725580159030848E-5	3.4511603180616957E-6
9	6.522693001136605E-4	0.0	0.0	1.725580159030848E-5	3.4511603180616957E-6
10-11	8.28278476334807E-4	0.0	1.7255801590308478E-6	2.0706961908370178E-5	3.4511603180616957E-6
12-13	0.0010284457747823854	0.0	3.4511603180616957E-6	2.7609282544493566E-5	3.4511603180616957E-6
14-15	0.0011768456684590383	0.0	3.4511603180616957E-6	3.278602302158611E-5	3.4511603180616957E-6
16-17	0.0013062641803863518	3.4511603180616957E-6	3.4511603180616957E-6	3.451160318061696E-5	5.1767404770925435E-6
18-19	0.001430505951836573	3.4511603180616957E-6	3.4511603180616957E-6	3.451160318061696E-5	1.0353480954185087E-5
20-21	0.001544394242332609	3.4511603180616957E-6	3.4511603180616957E-6	3.7962763498678654E-5	1.3804641272246783E-5
22-23	0.001658282532828645	3.4511603180616957E-6	3.4511603180616957E-6	3.7962763498678654E-5	1.5530221431277633E-5
24-25	0.001748012701098249	3.4511603180616957E-6	3.4511603180616957E-6	4.486508413480204E-5	1.725580159030848E-5
26-27	0.0018877846939797478	3.4511603180616957E-6	3.4511603180616957E-6	5.176740477092544E-5	2.0706961908370178E-5
28-29	0.0020775985114731407	3.4511603180616957E-6	3.4511603180616957E-6	5.176740477092544E-5	2.415812222643187E-5
30-31	0.002362319237713231	3.4511603180616957E-6	3.4511603180616957E-6	6.0395305566079675E-5	2.7609282544493566E-5
32-33	0.0027212399107916473	3.4511603180616957E-6	3.4511603180616957E-6	6.557204604317222E-5	3.106044286255526E-5
34-35	0.0032647976608863646	5.1767404770925435E-6	3.4511603180616957E-6	7.247436667929561E-5	3.106044286255526E-5
36-37	0.0038290623728894514	6.902320636123391E-6	3.4511603180616957E-6	7.765110715638815E-5	3.106044286255526E-5
38-39	0.00453827581825113	6.902320636123391E-6	3.4511603180616957E-6	7.937668731541901E-5	3.106044286255526E-5
40-41	0.005418321699356863	6.902320636123391E-6	3.4511603180616957E-6	8.282784763348071E-5	3.451160318061696E-5
42-43	0.006700427757516782	6.902320636123391E-6	3.4511603180616957E-6	8.62790079515424E-5	3.451160318061696E-5
44-45	0.008358710290345427	6.902320636123391E-6	3.4511603180616957E-6	8.62790079515424E-5	3.451160318061696E-5
46-47	0.010332773992276717	1.0353480954185087E-5	3.4511603180616957E-6	8.62790079515424E-5	3.451160318061696E-5
48-49	0.012834865222871448	1.0353480954185087E-5	3.4511603180616957E-6	8.62790079515424E-5	3.451160318061696E-5
50-51	0.016008207135329175	1.0353480954185087E-5	5.1767404770925435E-6	8.800458811057324E-5	3.451160318061696E-5
52-53	0.019916646195534047	1.0353480954185087E-5	6.902320636123391E-6	9.318132858766579E-5	3.451160318061696E-5
54-55	0.024756898541615578	1.3804641272246783E-5	6.902320636123391E-6	1.0698596985991257E-4	3.623718333964781E-5
56-57	0.030749838433929713	1.3804641272246783E-5	6.902320636123391E-6	1.1561387065506682E-4	3.96883436577095E-5
58-59	0.038093907590765	1.3804641272246783E-5	6.902320636123391E-6	1.1733945081409766E-4	4.141392381674035E-5
60-61	0.04730505447967166	1.3804641272246783E-5	6.902320636123391E-6	1.2424177145022104E-4	4.141392381674035E-5
62-63	0.05962224565483386	1.3804641272246783E-5	6.902320636123391E-6	1.3286967224537528E-4	4.141392381674035E-5
64-65	0.07621715004423352	1.3804641272246783E-5	6.902320636123391E-6	1.3459525240440613E-4	4.141392381674035E-5
66-67	0.09863243631004423	1.725580159030848E-5	6.902320636123391E-6	1.3459525240440613E-4	4.486508413480205E-5
68-69	0.12736334595790785	1.725580159030848E-5	6.902320636123391E-6	1.3459525240440613E-4	4.65906642938329E-5
70-71	0.16532265829626847	1.725580159030848E-5	6.902320636123391E-6	1.3632083256343697E-4	4.831624445286374E-5
72-73	0.21653097509566788	1.725580159030848E-5	6.902320636123391E-6	1.4494873335859122E-4	4.831624445286374E-5
74-75	0.2853522885782952	1.725580159030848E-5	6.902320636123391E-6	1.4494873335859122E-4	4.831624445286374E-5
76-77	0.3746407083271874	1.725580159030848E-5	6.902320636123391E-6	1.4839989367665293E-4	4.831624445286374E-5
78-79	0.4888637613740754	1.725580159030848E-5	6.902320636123391E-6	1.553022143127763E-4	4.831624445286374E-5
80-81	0.6310532920583762	1.725580159030848E-5	6.902320636123391E-6	1.6047895478986886E-4	4.831624445286374E-5
82-83	0.8112625303866039	1.725580159030848E-5	6.902320636123391E-6	1.622045349488997E-4	4.831624445286374E-5
84-85	1.0394601529374792	1.725580159030848E-5	6.902320636123391E-6	1.656556952669614E-4	4.831624445286374E-5
86-87	1.3240859722688225	1.725580159030848E-5	6.902320636123391E-6	1.725580159030848E-4	4.831624445286374E-5
88-89	1.6658008855815423	1.725580159030848E-5	6.902320636123391E-6	1.7773475638017733E-4	4.831624445286374E-5
90-91	2.0670189795181226	1.725580159030848E-5	6.902320636123391E-6	1.8808823733436241E-4	4.831624445286374E-5
92-93	2.5411549142348697	1.725580159030848E-5	6.902320636123391E-6	1.9153939765242413E-4	4.831624445286374E-5
94-95	3.0971592740166765	1.8981381749339327E-5	6.902320636123391E-6	1.984417182885475E-4	4.831624445286374E-5
96-97	3.7350199798024297	2.0706961908370174E-5	6.902320636123391E-6	2.1052077940176344E-4	5.176740477092544E-5
98-99	4.457530745869599	2.5883702385462715E-5	6.902320636123391E-6	2.1052077940176344E-4	5.521856508898713E-5
100-101	5.264077265681572	3.106044286255526E-5	6.902320636123391E-6	2.1052077940176344E-4	5.521856508898713E-5
102-103	6.1540590373430035	3.106044286255526E-5	8.627900795154238E-6	2.1052077940176344E-4	5.521856508898713E-5
104-105	7.136390407955449	3.106044286255526E-5	1.0353480954185087E-5	2.1397193971982515E-4	5.521856508898713E-5
106-107	8.203219987795316	3.106044286255526E-5	1.0353480954185087E-5	2.2087426035594852E-4	5.521856508898713E-5
108-109	9.35192316944072	3.451160318061696E-5	1.0353480954185087E-5	2.2777658099207193E-4	5.521856508898713E-5
110-111	10.54864061901116	3.96883436577095E-5	1.0353480954185087E-5	2.2777658099207193E-4	5.521856508898713E-5
112-113	11.808874948655362	4.486508413480204E-5	1.0353480954185087E-5	2.312277413101336E-4	5.521856508898713E-5
114-115	13.132339712066933	4.831624445286374E-5	1.0353480954185087E-5	2.3295332146916448E-4	5.521856508898713E-5
116-117	14.508448474970217	5.176740477092544E-5	1.0353480954185087E-5	2.3467890162819533E-4	5.521856508898713E-5
118-119	15.909629917584223	5.176740477092544E-5	1.0353480954185087E-5	2.3467890162819533E-4	5.521856508898713E-5
120-121	17.319556600444194	5.176740477092544E-5	1.0353480954185087E-5	2.3985564210528786E-4	5.521856508898713E-5
122-123	18.7473275077287	5.176740477092544E-5	1.0353480954185087E-5	2.502091230594729E-4	5.521856508898713E-5
124-125	20.190150650740435	5.176740477092544E-5	1.0353480954185087E-5	2.5366028337753466E-4	5.521856508898713E-5
126-127	21.63681838634649	5.176740477092544E-5	1.0353480954185087E-5	2.553858635365655E-4	5.521856508898713E-5
128-129	23.089599852455994	5.176740477092544E-5	1.0353480954185087E-5	2.5711144369559635E-4	5.694414524801798E-5
130-131	24.530757810614265	5.176740477092544E-5	1.0353480954185087E-5	2.588370238546272E-4	5.866972540704883E-5
132-133	25.964495774088704	5.176740477092544E-5	1.0353480954185087E-5	2.640137643317197E-4	5.866972540704883E-5
134-135	27.382147879320655	5.521856508898713E-5	1.0353480954185087E-5	2.72641665126874E-4	5.866972540704883E-5
136-137	28.77128822358494	5.521856508898713E-5	1.0353480954185087E-5	2.72641665126874E-4	5.866972540704883E-5
138-139	30.143127901174783	5.694414524801798E-5	1.2079061113215936E-5	2.778184056039665E-4	6.0395305566079675E-5
>>END_MODULE
