##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261623_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28975762
Sequences flagged as poor quality	0
Sequence length	150
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.449068638816126	32.0	32.0	32.0	32.0	32.0
2	31.673589257117726	32.0	32.0	32.0	32.0	32.0
3	35.80367046084931	37.0	37.0	37.0	32.0	37.0
4	36.29733271552962	37.0	37.0	37.0	37.0	37.0
5	36.50644362691825	37.0	37.0	37.0	37.0	37.0
6	40.034949727983	41.0	41.0	41.0	37.0	41.0
7	40.09802958762568	41.0	41.0	41.0	37.0	41.0
8	40.24342431443218	41.0	41.0	41.0	37.0	41.0
9	40.23969015896804	41.0	41.0	41.0	41.0	41.0
10-14	40.26592107569078	41.0	41.0	41.0	41.0	41.0
15-19	40.25024524290336	41.0	41.0	41.0	41.0	41.0
20-24	40.235210690921605	41.0	41.0	41.0	41.0	41.0
25-29	40.171529107672825	41.0	41.0	41.0	41.0	41.0
30-34	40.154969632895245	41.0	41.0	41.0	41.0	41.0
35-39	40.13928717388002	41.0	41.0	41.0	41.0	41.0
40-44	40.0628443248533	41.0	41.0	41.0	37.0	41.0
45-49	40.018922484247355	41.0	41.0	41.0	37.0	41.0
50-54	40.04905643551324	41.0	41.0	41.0	37.0	41.0
55-59	40.02098080457729	41.0	41.0	41.0	37.0	41.0
60-64	39.9810815467079	41.0	41.0	41.0	37.0	41.0
65-69	39.937081026549016	41.0	41.0	41.0	37.0	41.0
70-74	39.892089940551	41.0	41.0	41.0	37.0	41.0
75-79	39.47240582663538	41.0	40.2	41.0	36.0	41.0
80-84	39.84365331272392	41.0	41.0	41.0	37.0	41.0
85-89	39.82814382586383	41.0	41.0	41.0	37.0	41.0
90-94	39.78142724253464	41.0	41.0	41.0	37.0	41.0
95-99	39.72870361787207	41.0	41.0	41.0	37.0	41.0
100-104	39.66716800752298	41.0	41.0	41.0	37.0	41.0
105-109	39.55802727810921	41.0	41.0	41.0	37.0	41.0
110-114	39.46726384624501	41.0	41.0	41.0	37.0	41.0
115-119	39.36394867544812	41.0	41.0	41.0	37.0	41.0
120-124	39.22743032607737	41.0	41.0	41.0	37.0	41.0
125-129	39.06264477186139	41.0	41.0	41.0	37.0	41.0
130-134	38.85587506551165	41.0	41.0	41.0	32.0	41.0
135-139	38.620504758425334	41.0	41.0	41.0	32.0	41.0
140-144	38.36096774262571	41.0	41.0	41.0	32.0	41.0
145-149	38.07941667245886	41.0	39.4	41.0	31.0	41.0
150	34.25550896642511	37.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	16.0
13	21.0
14	45.0
15	43.0
16	59.0
17	143.0
18	401.0
19	1097.0
20	2771.0
21	5430.0
22	10542.0
23	19060.0
24	32985.0
25	57592.0
26	115034.0
27	108909.0
28	118376.0
29	151369.0
30	190138.0
31	235340.0
32	285634.0
33	345907.0
34	421594.0
35	524184.0
36	676520.0
37	929297.0
38	1411844.0
39	2793526.0
40	2.0537885E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.49908013802131	9.243715880453545	10.595657224916879	40.66154675660826
2	25.141841291142498	11.137356987757562	39.005918438280595	24.71488328281934
3	25.074343169991526	14.945839905780561	23.91728645479625	36.06253046943166
4	27.513058647136447	23.604301210669934	24.168255547551894	24.714384594641725
5	28.41511220361046	26.88022930614316	26.535309978571398	18.169348511674983
6	22.574993718887786	27.311822019508718	29.370273635618187	20.742910625985306
7	19.739511807332654	21.480362090453312	41.527731612101284	17.252394490112753
8	20.55256752608761	20.76922142438411	35.26535374367486	23.412857305853418
9	20.211377958374264	21.661671369188923	35.97048687714157	22.156463795295245
10-14	25.159292045965532	24.78091332172392	27.273078664617124	22.78671596769343
15-19	25.400385671637025	23.848866216249366	27.257808646884058	23.49293946522955
20-24	25.013850396201224	24.272811663801637	27.346242276469308	23.36709566352783
25-29	24.88243695363959	24.377493042647192	27.195598235524848	23.544471768188366
30-34	24.800272992948873	24.4527409533226	26.657695470534374	24.08929058319415
35-39	25.069100968976322	24.434538324793937	26.767849459230458	23.728511246999282
40-44	24.91125129686538	24.139007852489534	27.29570211323054	23.654038737414545
45-49	24.952147899339526	24.11044193109737	27.205751851036457	23.731658318526648
50-54	24.903499184400797	24.42158802097761	26.84787590114387	23.827036893477725
55-59	24.972298855398535	24.247995235612965	26.84493474645156	23.93477116253694
60-64	24.69041215996695	24.26980974638569	26.900035905816416	24.13974218783094
65-69	24.945053512989354	25.041363185590722	26.45068230360809	23.56290099781183
70-74	24.97431897085793	24.846000276991013	26.436715576782717	23.74296517536834
75-79	25.015640599681564	24.67724427842985	26.388894880530213	23.918220241358377
80-84	24.88620315295687	24.74703124232207	26.46612040314698	23.90064520157408
85-89	25.27933140298905	24.606318638559713	26.405327143354484	23.709022815096752
90-94	25.30518175342496	24.83431324097031	26.067078002396503	23.793427003208226
95-99	25.544273014482616	24.970394897097826	25.904845711446782	23.580486376972782
100-104	25.48182469370425	25.246287551939606	25.50979943597983	23.76208831837631
105-109	25.64149040073674	25.44417850602342	25.032007506633196	23.882323586606645
110-114	25.872007661001884	25.76076973255974	24.70519112035347	23.66203148608491
115-119	25.69203256439012	26.192982245558756	24.245745350575287	23.869239839475835
120-124	25.63368656856568	26.27268109431551	23.928072886852547	24.165559450266265
125-129	25.27961705048021	26.43559012816948	23.847889677408993	24.436903143941326
130-134	25.01972305788327	26.38583324734183	23.883654354868714	24.710789339906192
135-139	24.711649420362537	26.44000761450326	23.87791252972254	24.97043043541166
140-144	24.462697184225775	26.38141924843272	24.15017129366414	25.00571227367736
145-149	24.07769105379432	26.166435230358605	24.604269317320846	25.151604398526235
150	23.697694210609143	25.868936656018686	24.974568397860832	25.45880073551134
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	14.5
2	11.5
3	15.5
4	18.0
5	21.0
6	29.0
7	36.0
8	36.5
9	38.0
10	51.0
11	61.0
12	79.0
13	90.5
14	116.0
15	156.5
16	220.0
17	350.5
18	557.5
19	816.0
20	1493.5
21	2741.0
22	4080.5
23	6002.5
24	9438.5
25	15264.0
26	23291.5
27	33724.5
28	50845.0
29	76814.0
30	106285.5
31	135895.5
32	179578.5
33	238224.5
34	300639.5
35	361348.0
36	431351.0
37	533532.5
38	607440.0
39	620842.0
40	636894.5
41	712733.5
42	790726.0
43	877692.5
44	997095.0
45	1122270.5
46	1184052.0
47	1205659.0
48	1259333.0
49	1344532.5
50	1462429.5
51	1468212.5
52	1421918.5
53	1416095.0
54	1328933.5
55	1216961.0
56	1114794.5
57	999888.5
58	917277.0
59	815930.5
60	679584.0
61	544410.5
62	434382.0
63	334271.0
64	252099.5
65	191206.5
66	137673.0
67	103643.0
68	77709.5
69	55491.5
70	39394.5
71	26356.5
72	20245.5
73	14800.0
74	10015.0
75	6927.0
76	4916.0
77	3079.0
78	1719.5
79	1031.0
80	695.5
81	469.5
82	310.5
83	164.5
84	72.0
85	39.5
86	18.0
87	9.0
88	4.0
89	2.5
90	3.0
91	3.0
92	2.5
93	2.0
94	2.0
95	3.0
96	2.5
97	1.5
98	2.5
99	4.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0250049679452777
2	0.01239656786247761
3	0.0
4	0.0196232975684988
5	3.4511603180616957E-6
6	6.902320636123391E-6
7	0.003496025402196498
8	0.0017807987241198352
9	6.108553762969202E-4
10-14	2.905876987807948E-4
15-19	8.558877588793006E-5
20-24	1.9533567400229198E-4
25-29	0.04766949700925898
30-34	0.0012479395710111093
35-39	0.00465630550112884
40-44	0.03835412507874685
45-49	0.10681755323639117
50-54	8.821165772965694E-4
55-59	0.002712612009996493
60-64	0.0017676843149112007
65-69	0.0016537960244151647
70-74	3.209579095797377E-4
75-79	7.268143629837931E-4
80-84	0.002285358362620455
85-89	0.0014239487472322556
90-94	8.351807969709305E-4
95-99	0.0023799201553353455
100-104	0.003636142511109803
105-109	7.433799325104894E-4
110-114	0.002598033487436845
115-119	1.2907339589550743E-4
120-124	0.0013680399500796562
125-129	0.0011368122087695226
130-134	1.1043713017797426E-4
135-139	0.0018284247365090863
140-144	0.001499874274229613
145-149	0.014067619688483085
150	6.902320636123391E-6
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.8975762E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.09082764994792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.8157607612153	19.892733304432433
2	13.157722995781345	7.392226578805004
3	4.9396936351381395	4.162802476446306
4	2.68125335002181	3.0127450296523257
5	1.6893498960502116	2.372761838520196
6	1.1054246603871305	1.8631376168962255
7	0.7715227270770122	1.5170898368036707
8	0.6197731091147266	1.3927951672171321
9	0.47432617927419496	1.1991793456664739
>10	3.108028784349396	16.895158341817627
>50	0.3333565761037018	6.511589396015709
>100	0.2534693056713354	14.290471248917777
>500	0.029512301668957297	5.704795942146327
>1k	0.01932394639104138	10.252645212810956
>5k	0.0012518416558736664	2.3812914903188274
>10k+	2.2993010005758536E-4	1.1585771735332127
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG	91394	0.3154153461089306	TruSeq Adapter, Index 7 (97% over 34bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1733945081409766E-4	0.0	0.0	0.0	0.0
2	1.691068555850231E-4	0.0	0.0	3.4511603180616957E-6	1.3804641272246783E-5
3	2.415812222643187E-4	0.0	0.0	6.902320636123391E-6	4.141392381674035E-5
4	4.072369175312801E-4	0.0	0.0	1.0353480954185087E-5	5.176740477092544E-5
5	5.10771727073131E-4	0.0	0.0	1.3804641272246783E-5	5.521856508898713E-5
6	6.281111778872286E-4	0.0	0.0	1.3804641272246783E-5	5.866972540704883E-5
7	6.764274223400924E-4	0.0	0.0	1.3804641272246783E-5	5.866972540704883E-5
8	7.074878652026476E-4	3.4511603180616957E-6	0.0	2.415812222643187E-5	6.557204604317222E-5
9	9.594225684211514E-4	3.4511603180616957E-6	0.0	3.106044286255526E-5	7.592552699735731E-5
10-11	0.001147510805755514	3.4511603180616957E-6	0.0	3.278602302158611E-5	7.937668731541901E-5
12-13	0.0013580315851572772	3.4511603180616957E-6	0.0	3.451160318061696E-5	8.110226747444985E-5
14-15	0.0015133337994700535	5.1767404770925435E-6	0.0	3.451160318061696E-5	8.97301682696041E-5
16-17	0.001634124410602213	1.0353480954185087E-5	0.0	3.451160318061696E-5	8.97301682696041E-5
18-19	0.001748012701098249	1.5530221431277633E-5	0.0	3.451160318061696E-5	8.97301682696041E-5
20-21	0.0018463707701630073	1.725580159030848E-5	0.0	4.1413923816740355E-5	9.145574842863494E-5
22-23	0.0019481799995458274	1.725580159030848E-5	0.0	5.004182461189459E-5	9.318132858766579E-5
24-25	0.002029282267020277	1.725580159030848E-5	0.0	6.729762620220306E-5	9.318132858766579E-5
26-27	0.00211728685513085	1.725580159030848E-5	0.0	7.247436667929561E-5	9.490690874669664E-5
28-29	0.0022294495654678554	1.725580159030848E-5	0.0	7.592552699735731E-5	9.835806906475833E-5
30-31	0.0024451470853467114	1.725580159030848E-5	0.0	7.592552699735731E-5	1.0353480954185087E-4
32-33	0.0027160631703145545	1.725580159030848E-5	0.0	7.592552699735731E-5	1.1733945081409766E-4
34-35	0.0031836953934119144	1.725580159030848E-5	0.0	7.592552699735731E-5	1.4149757304052953E-4
36-37	0.0036634066776224904	1.8981381749339327E-5	0.0	8.282784763348071E-5	1.622045349488997E-4
38-39	0.00433465735948549	2.0706961908370174E-5	0.0	9.490690874669664E-5	2.0706961908370175E-4
40-41	0.005131875392957742	2.0706961908370174E-5	0.0	1.0353480954185087E-4	2.2605100083304108E-4
42-43	0.006327702443166119	2.588370238546272E-5	0.0	1.0353480954185087E-4	2.3467890162819533E-4
44-45	0.007892803647407099	2.7609282544493566E-5	0.0	1.0526038970088173E-4	2.415812222643187E-4
46-47	0.009832355746157772	2.7609282544493566E-5	0.0	1.0871155001894342E-4	2.4848354290044213E-4
48-49	0.012244716808482898	2.7609282544493566E-5	0.0	1.1043713017797426E-4	2.553858635365655E-4
50-51	0.015380095957441949	2.7609282544493566E-5	0.0	1.1388829049603596E-4	2.674649246497814E-4
52-53	0.019350655903371927	2.7609282544493566E-5	1.7255801590308478E-6	1.1561387065506682E-4	2.7954398576299737E-4
54-55	0.02418055476849927	2.7609282544493566E-5	3.4511603180616957E-6	1.2079061113215935E-4	2.8644630639912074E-4
56-57	0.030316717814012968	2.7609282544493566E-5	3.4511603180616957E-6	1.2424177145022104E-4	3.054276881484601E-4
58-59	0.03775396829943592	2.7609282544493566E-5	3.4511603180616957E-6	1.2424177145022104E-4	3.071532683074909E-4
60-61	0.047044491875658005	2.7609282544493566E-5	3.4511603180616957E-6	1.2424177145022104E-4	3.071532683074909E-4
62-63	0.05956012476910875	2.7609282544493566E-5	3.4511603180616957E-6	1.2424177145022104E-4	3.140555889436143E-4
64-65	0.07660540558001547	2.7609282544493566E-5	3.4511603180616957E-6	1.2769293176828275E-4	3.192323294207069E-4
66-67	0.099402045060972	2.7609282544493566E-5	3.4511603180616957E-6	1.2769293176828275E-4	3.382137111700462E-4
68-69	0.12926148413284178	2.7609282544493566E-5	3.4511603180616957E-6	1.2769293176828275E-4	3.675485738735706E-4
70-71	0.1685805536365187	2.9334862703524413E-5	3.4511603180616957E-6	1.2769293176828275E-4	3.7272531435066314E-4
72-73	0.22133153909809172	3.4511603180616954E-5	3.4511603180616957E-6	1.3114409208634444E-4	3.7272531435066314E-4
74-75	0.2922994742985534	3.7962763498678654E-5	3.4511603180616957E-6	1.3114409208634444E-4	3.7617647466872483E-4
76-77	0.38409688759867644	3.7962763498678654E-5	3.4511603180616957E-6	1.3459525240440613E-4	3.7790205482775567E-4
78-79	0.5012102874119411	3.7962763498678654E-5	3.4511603180616957E-6	1.3804641272246784E-4	3.796276349867865E-4
80-81	0.6478915032501993	3.7962763498678654E-5	6.902320636123391E-6	1.4149757304052953E-4	3.796276349867865E-4
82-83	0.833420705208719	3.7962763498678654E-5	6.902320636123391E-6	1.4839989367665293E-4	3.796276349867865E-4
84-85	1.0683308345782243	4.141392381674035E-5	6.902320636123391E-6	1.6565569526696142E-4	3.8135321514581736E-4
86-87	1.3623955773794663	4.486508413480205E-5	1.0353480954185087E-5	1.9153939765242413E-4	3.8652995562290994E-4
88-89	1.7150765526028273	4.486508413480205E-5	1.725580159030848E-5	2.0016729844757835E-4	3.8652995562290994E-4
90-91	2.1301976458807195	4.486508413480205E-5	2.0706961908370174E-5	2.122463595607943E-4	3.8652995562290994E-4
92-93	2.622326204915681	4.486508413480205E-5	2.0706961908370174E-5	2.1397193971982515E-4	3.9860901673612585E-4
94-95	3.1987459035589816	4.486508413480205E-5	2.243254206740102E-5	2.1742310003788684E-4	4.227671389625577E-4
96-97	3.858003803316717	4.486508413480205E-5	2.415812222643187E-5	2.1742310003788684E-4	4.244927191215886E-4
98-99	4.603956575844322	4.486508413480205E-5	2.415812222643187E-5	2.2087426035594852E-4	4.2621829928061946E-4
100-101	5.436954513914078	4.486508413480205E-5	2.415812222643187E-5	2.2605100083304108E-4	4.279438794396503E-4
102-103	6.356174515790128	4.486508413480205E-5	2.415812222643187E-5	2.2777658099207193E-4	4.279438794396503E-4
104-105	7.370867416705037	4.486508413480205E-5	2.415812222643187E-5	2.3467890162819533E-4	4.279438794396503E-4
106-107	8.47427929591636	4.486508413480205E-5	2.415812222643187E-5	2.502091230594729E-4	4.279438794396503E-4
108-109	9.658379303363963	4.486508413480205E-5	2.415812222643187E-5	2.5366028337753466E-4	4.279438794396503E-4
110-111	10.897747917725166	4.486508413480205E-5	2.415812222643187E-5	2.5711144369559635E-4	4.279438794396503E-4
112-113	12.198623111274866	4.486508413480205E-5	2.415812222643187E-5	2.622881841726889E-4	4.348462000757737E-4
114-115	13.565852038679775	4.486508413480205E-5	2.415812222643187E-5	2.6573934449075057E-4	4.3829736039383536E-4
116-117	14.985262510093781	4.486508413480205E-5	2.415812222643187E-5	2.6573934449075057E-4	4.4174852071189705E-4
118-119	16.439055166176473	4.486508413480205E-5	2.415812222643187E-5	2.6573934449075057E-4	4.4174852071189705E-4
120-121	17.902159052797302	4.486508413480205E-5	2.415812222643187E-5	2.6573934449075057E-4	4.4174852071189705E-4
122-123	19.385024283399346	4.486508413480205E-5	2.415812222643187E-5	2.6573934449075057E-4	4.4174852071189705E-4
124-125	20.887592188257205	4.486508413480205E-5	2.415812222643187E-5	2.7436724528590484E-4	4.5210200166608216E-4
126-127	22.397371292599658	4.486508413480205E-5	2.415812222643187E-5	2.778184056039665E-4	4.7280896357445234E-4
128-129	23.911509212423816	4.486508413480205E-5	2.415812222643187E-5	2.7954398576299737E-4	4.7280896357445234E-4
130-131	25.416812161833743	4.486508413480205E-5	2.415812222643187E-5	2.7954398576299737E-4	4.7280896357445234E-4
132-133	26.9168193057356	4.65906642938329E-5	2.415812222643187E-5	2.7954398576299737E-4	4.831624445286374E-4
134-135	28.40609334104829	4.831624445286374E-5	2.415812222643187E-5	2.7954398576299737E-4	4.883391850057299E-4
136-137	29.862840190363244	4.831624445286374E-5	2.415812222643187E-5	2.7954398576299737E-4	5.00418246118946E-4
138-139	31.307449308839573	5.004182461189459E-5	2.588370238546272E-5	2.812695659220282E-4	5.142228873911927E-4
>>END_MODULE
