##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261643_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	64824386
Sequences flagged as poor quality	0
Sequence length	150
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.288389156512796	32.0	32.0	32.0	32.0	32.0
2	31.497978430524586	32.0	32.0	32.0	32.0	32.0
3	35.370686920196974	37.0	37.0	37.0	32.0	37.0
4	36.01794619389067	37.0	37.0	37.0	32.0	37.0
5	36.211853900166524	37.0	37.0	37.0	37.0	37.0
6	39.72163190253742	41.0	41.0	41.0	37.0	41.0
7	39.79598993810755	41.0	41.0	41.0	37.0	41.0
8	39.90917223650988	41.0	41.0	41.0	37.0	41.0
9	39.92750394889973	41.0	41.0	41.0	37.0	41.0
10-14	39.88499495544778	41.0	41.0	41.0	37.0	41.0
15-19	39.86931325813098	41.0	41.0	41.0	37.0	41.0
20-24	39.8443854724671	41.0	41.0	41.0	37.0	41.0
25-29	39.78749664979472	41.0	41.0	41.0	37.0	41.0
30-34	39.79821705060191	41.0	41.0	41.0	37.0	41.0
35-39	39.66833431172029	41.0	41.0	41.0	37.0	41.0
40-44	39.63734822571247	41.0	41.0	41.0	37.0	41.0
45-49	39.6092458538674	41.0	41.0	41.0	37.0	41.0
50-54	39.53913360320914	41.0	41.0	41.0	37.0	41.0
55-59	39.484119892782324	41.0	41.0	41.0	37.0	41.0
60-64	39.45473434642327	41.0	41.0	41.0	37.0	41.0
65-69	39.39597963951406	41.0	41.0	41.0	37.0	41.0
70-74	39.309510788116064	41.0	41.0	41.0	37.0	41.0
75-79	38.972565589745805	41.0	40.2	41.0	35.0	41.0
80-84	39.38596752462877	41.0	41.0	41.0	37.0	41.0
85-89	39.34445661853242	41.0	41.0	41.0	37.0	41.0
90-94	39.23603748132686	41.0	41.0	41.0	37.0	41.0
95-99	39.12989611039278	41.0	41.0	41.0	37.0	41.0
100-104	38.98172750606538	41.0	41.0	41.0	37.0	41.0
105-109	38.79600153250969	41.0	41.0	41.0	34.0	41.0
110-114	38.58478368001819	41.0	41.0	41.0	32.0	41.0
115-119	38.36194869319703	41.0	39.4	41.0	32.0	41.0
120-124	38.03879642145781	41.0	37.0	41.0	32.0	41.0
125-129	37.69458916278821	41.0	37.0	41.0	29.0	41.0
130-134	37.299160269717014	41.0	37.0	41.0	27.0	41.0
135-139	36.82694941992971	41.0	37.0	41.0	27.0	41.0
140-144	36.227741498392284	41.0	37.0	41.0	23.0	41.0
145-149	35.67741242624342	41.0	33.0	41.0	22.0	41.0
150	30.289650040032775	32.0	22.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	12.0
10	24.0
11	219.0
12	958.0
13	1396.0
14	3026.0
15	7687.0
16	21428.0
17	43736.0
18	63496.0
19	83215.0
20	104552.0
21	128713.0
22	155407.0
23	187361.0
24	224019.0
25	269164.0
26	305981.0
27	348308.0
28	406998.0
29	474826.0
30	557551.0
31	661971.0
32	796077.0
33	973994.0
34	1212958.0
35	1557181.0
36	2080890.0
37	3012639.0
38	4991245.0
39	1.1257499E7
40	3.4891848E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.51330547589948	17.52320261842145	10.84119759126109	38.12229431441798
2	26.404391680478632	25.246273808008358	28.146627396136463	20.20270711537654
3	23.45496685351818	25.974773333121053	25.765757518452432	24.804502294908332
4	26.19384053079773	31.091066740825713	20.409779119703344	22.305313608673213
5	28.626136621057753	32.36847114930272	19.540027493186294	19.46536473645323
6	23.508506524100866	35.66909946766151	19.198639733685738	21.62375427455189
7	22.730871227558232	20.342640567180926	32.482938702477256	24.44354950278359
8	22.78210062284946	23.892921285013202	24.746324368056783	28.578653724080556
9	22.963298025792017	22.370102080115334	27.85581462378306	26.81078527030959
10-14	25.50475814721183	26.415279434601153	22.70718969399245	25.372772724194565
15-19	25.38545481035988	25.949885587626277	23.485986323956244	25.1786732780576
20-24	25.19598079763919	25.928562638666435	23.68085343814856	25.19460312554581
25-29	25.186043936994977	25.691170779579863	23.62010249797388	25.50268278545128
30-34	24.921649394350545	25.922673013670895	23.697686379420524	25.457991212558035
35-39	24.86030926295658	25.99198727062218	23.6494257756226	25.498277690798638
40-44	24.91780924587981	25.687080930856737	23.773668629098495	25.621441194164962
45-49	24.902832787468643	25.684048984169788	23.823653337975518	25.589464890386054
50-54	24.975801923720297	25.835842341216985	23.70587692074053	25.482478814322185
55-59	25.092223268146473	25.712884437278603	23.689548624902795	25.505343669672122
60-64	25.035687866244576	25.61714686478027	23.977533286811653	25.369631982163497
65-69	25.224438240364194	25.581222102454333	24.071836577643964	25.122503079537513
70-74	25.204699694841832	25.581445464171242	23.98357827163854	25.230276569348387
75-79	25.246303710690288	25.712329639889088	23.84978130933179	25.19158534008884
80-84	25.108408985096908	25.916294938172875	23.855303521909057	25.11999255482116
85-89	25.10659909941946	25.90193120434528	23.64811823566831	25.34335146056695
90-94	25.109747688424605	26.176778002309486	23.682556044079348	25.030918265186564
95-99	25.112843187595946	26.300432559034263	23.640410871146116	24.94631338222367
100-104	25.533537882129636	26.375644898510775	23.342153715681096	24.748663503678493
105-109	25.674970742148062	26.70092462871504	23.13362162153623	24.490483007600666
110-114	26.009042991196484	26.754592946760848	22.89289942952429	24.343464632518376
115-119	26.290506898757382	26.761694557985336	22.90691893968525	24.04087960357203
120-124	26.684905737885217	26.946518099940807	22.803871025447215	23.56470513672676
125-129	27.252555591314437	27.07345443566225	22.593119336632363	23.080870636390955
130-134	27.86469557128328	27.09623741695023	22.385243550301155	22.653823461465333
135-139	28.044326409403318	27.178608612684197	22.555674165096057	22.221390812816434
140-144	28.281227229196155	27.34972635496698	22.37648545204398	21.992560963792886
145-149	28.643680467230702	27.088099116444937	22.58350309816642	21.68471731815794
150	28.12986294722608	27.403943072522168	22.78817911263375	21.678014867618
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	234.0
1	148.5
2	53.0
3	47.5
4	70.0
5	79.0
6	88.0
7	111.5
8	124.0
9	153.0
10	186.0
11	214.0
12	270.5
13	321.5
14	394.5
15	552.0
16	725.0
17	944.0
18	1267.0
19	1807.0
20	3043.0
21	4575.0
22	6417.0
23	9220.0
24	13053.0
25	17873.5
26	24682.0
27	34766.5
28	46759.0
29	65217.5
30	89300.5
31	120720.5
32	166589.5
33	248792.5
34	390540.5
35	503653.5
36	610976.0
37	817828.5
38	992913.5
39	1081492.0
40	1182988.0
41	1394420.0
42	1644536.0
43	1897003.5
44	2087856.0
45	2289529.0
46	2552668.5
47	2735169.5
48	2949526.5
49	3215571.0
50	3488912.0
51	3497348.5
52	3333229.5
53	3216315.0
54	3020633.0
55	2929517.5
56	2794738.0
57	2494701.5
58	2241932.0
59	1954052.5
60	1645955.5
61	1376217.0
62	1124936.0
63	921776.5
64	730890.0
65	566929.5
66	437819.0
67	343172.0
68	281977.0
69	229772.5
70	183088.0
71	154829.5
72	145045.0
73	122965.5
74	95801.0
75	71655.5
76	52485.5
77	39897.0
78	29562.0
79	22746.5
80	16928.0
81	12532.5
82	9106.5
83	5756.5
84	3708.0
85	2414.0
86	1496.0
87	1049.0
88	808.5
89	650.5
90	617.5
91	615.0
92	648.5
93	659.0
94	624.5
95	638.0
96	724.0
97	843.5
98	993.0
99	1368.5
100	4391.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4807203264524557
2	0.01236108892724414
3	0.0060656185775519724
4	0.01347640994239421
5	0.011722440379149908
6	0.0072904662760708595
7	0.01271589367618538
8	0.008009331550012676
9	0.01134603881940355
10-14	0.010916570810250327
15-19	0.008249981727555429
20-24	0.007555489997236534
25-29	0.00915396252268398
30-34	0.007676740663613845
35-39	0.006750237480074243
40-44	0.006947694036006759
45-49	0.008412574860331111
50-54	0.006084130129670646
55-59	0.007305275517765799
60-64	0.008602626795416159
65-69	0.007327797906176852
70-74	0.009962917350270004
75-79	0.006900798103972786
80-84	0.007696794845075741
85-89	0.006818421697044689
90-94	0.007611641705329843
95-99	0.008822297213891082
100-104	0.008372157971538674
105-109	0.008556656440988119
110-114	0.005996508782975592
115-119	0.005011385684393524
120-124	0.05000772394512152
125-129	0.0058780348494160825
130-134	0.00395067374799354
135-139	0.004213537788078702
140-144	0.0027693281969535352
145-149	0.004250252366447405
150	2.1596810805118925E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	6.4824386E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.601661231791095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.94536126602404	12.967790089153322
2	15.035745093216333	6.195226535560162
3	6.441973510106852	3.9814606775818064
4	3.6909092542515483	3.041554483734924
5	2.2745805142804008	2.343006859981899
6	1.5374644909028619	1.9004593558493352
7	1.1324885195603072	1.6331801380211852
8	0.8036200654860989	1.3244726678571506
9	0.6943156377230711	1.2873650000675134
>10	4.504633374065597	18.022306190429973
>50	0.49604262185127845	7.121435172227349
>100	0.36731209129112946	14.90236273579438
>500	0.04172833619851567	5.936807309237241
>1k	0.030131509573650794	12.187787416859862
>5k	0.0027027206499395107	3.776999197771257
>10k+	9.909948187765963E-4	3.3777861698729836
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCCACTAGCCTCTGGCAAAATGAAGTGGGTAACCTTTCTCCTCCTCCTC	76167	0.11749744918524953	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3139440148341707E-5	0.0	0.0	0.0	0.0
2	2.6224698834787268E-5	1.5426293432227803E-6	0.0	0.0	3.0852586864455607E-6
3	4.165099226701507E-5	3.0852586864455607E-6	0.0	0.0	3.0852586864455607E-6
4	1.1106931271204019E-4	6.170517372891121E-6	0.0	0.0	3.0852586864455607E-6
5	1.5426293432227803E-4	6.170517372891121E-6	0.0	1.5426293432227803E-6	4.627888029668341E-6
6	2.113402200215209E-4	6.170517372891121E-6	0.0	4.627888029668341E-6	4.627888029668341E-6
7	2.4065017754275374E-4	7.713146716113902E-6	0.0	4.627888029668341E-6	6.170517372891121E-6
8	2.7150276440720937E-4	7.713146716113902E-6	0.0	6.170517372891121E-6	6.170517372891121E-6
9	3.810294477760268E-4	7.713146716113902E-6	0.0	9.255776059336682E-6	6.170517372891121E-6
10-11	5.291218647254137E-4	1.0027090730948071E-5	0.0	1.3883664089005025E-5	6.170517372891121E-6
12-13	6.633306175857956E-4	1.3112349417393632E-5	0.0	2.2368125476730318E-5	6.170517372891121E-6
14-15	8.345624746835242E-4	1.3883664089005023E-5	0.0	2.4682069491564486E-5	6.9418320445025116E-6
16-17	0.0010266198279147605	1.3883664089005023E-5	0.0	2.4682069491564486E-5	7.713146716113902E-6
18-19	0.0012464445093240066	1.3883664089005023E-5	0.0	2.5453384163175875E-5	8.484461387725293E-6
20-21	0.0014462150092713565	1.3883664089005023E-5	0.0	2.7767328178010046E-5	1.0798405402559462E-5
22-23	0.0016698962640386598	1.4654978760616414E-5	0.0	2.930995752123283E-5	1.2341034745782243E-5
24-25	0.001974565559325159	1.5426293432227803E-5	0.0	3.0081272192844218E-5	1.4654978760616414E-5
26-27	0.002392618111338532	1.5426293432227803E-5	0.0	3.4709160222512554E-5	1.5426293432227803E-5
28-29	0.0028237830127692994	1.5426293432227803E-5	0.0	3.93370482521809E-5	1.6968922775450586E-5
30-31	0.003355218821509547	1.6968922775450586E-5	0.0	4.396493628184924E-5	1.6968922775450586E-5
32-33	0.004036289676542405	1.6968922775450586E-5	0.0	5.244939766957453E-5	2.0054181461896143E-5
34-35	0.004781379649319008	1.7740237447061975E-5	0.0	5.939122971407704E-5	2.3139440148341707E-5
36-37	0.00567070546568694	1.8511552118673364E-5	0.0	6.479043241535678E-5	2.5453384163175875E-5
38-39	0.006807623291642129	1.8511552118673364E-5	0.0	7.09609497882479E-5	2.7767328178010046E-5
40-41	0.008141226358858222	1.8511552118673364E-5	0.0	7.636015248952763E-5	2.7767328178010046E-5
42-43	0.009806494734867215	1.8511552118673364E-5	0.0	7.713146716113901E-5	2.930995752123283E-5
44-45	0.011732467469880856	1.9282866790284754E-5	0.0	8.253066986241875E-5	3.085258686445561E-5
46-47	0.014039469652670524	2.0825496133507536E-5	0.0	9.41003899365896E-5	3.3166530879289775E-5
48-49	0.016793063030323185	2.1596810805118925E-5	0.0	1.0335616599592629E-4	3.625178956573534E-5
50-51	0.020331083429004634	2.1596810805118925E-5	0.0	1.1106931271204019E-4	3.856573358056951E-5
52-53	0.024515465522496428	2.1596810805118925E-5	0.0	1.1261194205526296E-4	3.856573358056951E-5
54-55	0.029431825239347426	2.1596810805118925E-5	0.0	1.2032508877137687E-4	4.010836292379229E-5
56-57	0.03527376256213209	2.2368125476730318E-5	0.0	1.3112349417393633E-4	4.010836292379229E-5
58-59	0.04247321370695281	2.5453384163175875E-5	1.5426293432227803E-6	1.3729401154682745E-4	4.087967759540368E-5
60-61	0.05123997626448787	2.6996013506398657E-5	1.5426293432227803E-6	1.4346452891971856E-4	4.165099226701507E-5
62-63	0.062432523464240754	2.7767328178010046E-5	1.5426293432227803E-6	1.4346452891971856E-4	4.165099226701507E-5
64-65	0.07624676923280076	2.7767328178010046E-5	1.5426293432227803E-6	1.4654978760616415E-4	4.165099226701507E-5
66-67	0.09355584177843196	2.7767328178010046E-5	1.5426293432227803E-6	1.480924169493869E-4	4.165099226701507E-5
68-69	0.11478627811453548	2.7767328178010046E-5	1.5426293432227803E-6	1.4886373162099832E-4	4.165099226701507E-5
70-71	0.1404525451270761	2.7767328178010046E-5	1.5426293432227803E-6	1.6043345169516914E-4	4.396493628184924E-5
72-73	0.1724489916495314	3.085258686445561E-5	1.5426293432227803E-6	1.6891791308289443E-4	4.936413898312897E-5
74-75	0.2137343807622027	3.085258686445561E-5	1.5426293432227803E-6	1.7354580111256278E-4	5.2449397669574535E-5
76-77	0.2652489450497842	3.779441890895812E-5	1.5426293432227803E-6	1.7663105979900837E-4	5.3992027012797314E-5
78-79	0.32907292018161194	3.856573358056951E-5	1.5426293432227803E-6	1.8203026250028807E-4	5.784860037085426E-5
80-81	0.4073914406223609	3.856573358056951E-5	1.5426293432227803E-6	1.8434420651512225E-4	6.170517372891121E-5
82-83	0.5023325635510069	4.010836292379229E-5	1.5426293432227803E-6	1.882007798731792E-4	6.324780307213399E-5
84-85	0.6199325975875807	4.010836292379229E-5	1.5426293432227803E-6	1.9437129724607033E-4	6.324780307213399E-5
86-87	0.7682116726874976	4.010836292379229E-5	1.5426293432227803E-6	1.9745655593251588E-4	6.324780307213399E-5
88-89	0.948256571223058	4.010836292379229E-5	1.5426293432227803E-6	2.0285575863379562E-4	6.633306175857955E-5
90-91	1.1626442555121155	4.165099226701507E-5	1.5426293432227803E-6	2.0825496133507535E-4	6.787569110180234E-5
92-93	1.4161198534144233	4.473625095346063E-5	1.5426293432227803E-6	2.0979759067829811E-4	7.09609497882479E-5
94-95	1.7163448335630975	4.473625095346063E-5	1.5426293432227803E-6	2.1673942272280064E-4	7.250357913147068E-5
96-97	2.0712976749212864	4.473625095346063E-5	1.5426293432227803E-6	2.190533667376348E-4	7.250357913147068E-5
98-99	2.4897906476121503	4.6278880296683414E-5	1.5426293432227803E-6	2.3062308681180566E-4	7.481752314630484E-5
100-101	2.970913600323187	4.782150963990619E-5	1.5426293432227803E-6	2.3370834549825122E-4	7.86740965043618E-5
102-103	3.506985010239819	4.859282431151758E-5	1.5426293432227803E-6	2.4219280688597653E-4	8.021672584758458E-5
104-105	4.109758170328061	5.090676832635175E-5	1.5426293432227803E-6	2.445067509008107E-4	8.021672584758458E-5
106-107	4.78662690302998	5.090676832635175E-5	1.5426293432227803E-6	2.4990595360209044E-4	8.175935519080736E-5
108-109	5.544810713671858	5.090676832635175E-5	1.5426293432227803E-6	2.537625269601474E-4	8.330198453403014E-5
110-111	6.370944261624013	5.2449397669574535E-5	1.5426293432227803E-6	2.591617296614271E-4	8.407329920564154E-5
112-113	7.257388446378806	5.2449397669574535E-5	1.5426293432227803E-6	2.6533224703431825E-4	8.484461387725292E-5
114-115	8.198451736974416	5.2449397669574535E-5	1.5426293432227803E-6	2.66874876377541E-4	8.484461387725292E-5
116-117	9.196859188145647	5.3992027012797314E-5	1.5426293432227803E-6	2.7073144973559796E-4	8.63872432204757E-5
118-119	10.26137216324733	5.630597102763148E-5	1.5426293432227803E-6	2.7767328178010043E-4	8.71585578920871E-5
120-121	11.385257085196303	5.707728569924287E-5	1.5426293432227803E-6	2.892430018542713E-4	8.947250190692127E-5
122-123	12.561320673365113	5.707728569924287E-5	1.5426293432227803E-6	2.915569458691055E-4	9.101513125014405E-5
124-125	13.760148842751862	5.707728569924287E-5	1.5426293432227803E-6	2.961848338987738E-4	9.41003899365896E-5
126-127	14.981844178207874	5.707728569924287E-5	1.5426293432227803E-6	3.000414072568308E-4	9.718564862303516E-5
128-129	16.247600864279686	5.707728569924287E-5	1.5426293432227803E-6	3.069832393013333E-4	9.872827796625794E-5
130-131	17.556127720207023	5.784860037085426E-5	1.5426293432227803E-6	3.154677006890586E-4	9.872827796625794E-5
132-133	18.884571463584706	5.861991504246566E-5	3.0852586864455607E-6	3.2009558871872693E-4	9.872827796625794E-5
134-135	20.222832500102662	5.861991504246566E-5	3.0852586864455607E-6	3.2549479142000663E-4	9.872827796625794E-5
136-137	21.547324798417684	5.9391229714077046E-5	3.0852586864455607E-6	3.285800501064522E-4	9.872827796625794E-5
138-139	22.87896718373854	6.0933859057299825E-5	3.856573358056951E-6	3.332079381361205E-4	9.949959263786932E-5
>>END_MODULE
