##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261643_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	64824386
Sequences flagged as poor quality	0
Sequence length	150
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.477840175763486	32.0	32.0	32.0	32.0	32.0
2	31.76250280257186	32.0	32.0	32.0	32.0	32.0
3	35.96991157000701	37.0	37.0	37.0	32.0	37.0
4	36.43396440345767	37.0	37.0	37.0	37.0	37.0
5	36.59993345714065	37.0	37.0	37.0	37.0	37.0
6	40.22028748563851	41.0	41.0	41.0	37.0	41.0
7	40.30292967217615	41.0	41.0	41.0	41.0	41.0
8	40.366951535800126	41.0	41.0	41.0	41.0	41.0
9	40.347862685502335	41.0	41.0	41.0	41.0	41.0
10-14	40.398568288791814	41.0	41.0	41.0	41.0	41.0
15-19	40.37048832518058	41.0	41.0	41.0	41.0	41.0
20-24	40.353290053530166	41.0	41.0	41.0	41.0	41.0
25-29	40.31667461686409	41.0	41.0	41.0	41.0	41.0
30-34	40.27310259136122	41.0	41.0	41.0	41.0	41.0
35-39	40.23850892471238	41.0	41.0	41.0	41.0	41.0
40-44	40.23430563615365	41.0	41.0	41.0	41.0	41.0
45-49	40.20899763863556	41.0	41.0	41.0	41.0	41.0
50-54	40.15378781682559	41.0	41.0	41.0	41.0	41.0
55-59	40.152895359471664	41.0	41.0	41.0	41.0	41.0
60-64	40.11337134454309	41.0	41.0	41.0	41.0	41.0
65-69	40.077694351011665	41.0	41.0	41.0	41.0	41.0
70-74	40.055778422644835	41.0	41.0	41.0	38.6	41.0
75-79	39.87894104234169	41.0	41.0	41.0	37.0	41.0
80-84	40.05621817382119	41.0	41.0	41.0	40.2	41.0
85-89	40.045844441935785	41.0	41.0	41.0	40.2	41.0
90-94	40.01156553029288	41.0	41.0	41.0	37.0	41.0
95-99	39.94976953271875	41.0	41.0	41.0	37.0	41.0
100-104	39.92798743361796	41.0	41.0	41.0	37.0	41.0
105-109	39.8637492532517	41.0	41.0	41.0	37.0	41.0
110-114	39.77909195777033	41.0	41.0	41.0	37.0	41.0
115-119	39.65443679481977	41.0	41.0	41.0	37.0	41.0
120-124	39.518799440691964	41.0	41.0	41.0	37.0	41.0
125-129	39.393460451750364	41.0	41.0	41.0	37.0	41.0
130-134	39.23385860685206	41.0	41.0	41.0	37.0	41.0
135-139	39.00675624447873	41.0	41.0	41.0	37.0	41.0
140-144	38.824729196818	41.0	41.0	41.0	32.0	41.0
145-149	38.58897268691446	41.0	41.0	41.0	32.0	41.0
150	35.400982802984046	41.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	41.0
13	90.0
14	119.0
15	180.0
16	294.0
17	578.0
18	1021.0
19	2434.0
20	5489.0
21	10355.0
22	18697.0
23	32084.0
24	52841.0
25	82594.0
26	121526.0
27	167600.0
28	220722.0
29	284385.0
30	352736.0
31	431361.0
32	520723.0
33	623058.0
34	755977.0
35	937868.0
36	1216317.0
37	1690084.0
38	2681397.0
39	5987053.0
40	4.8626762E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.16591730285627	10.597935394373218	7.879919310641437	40.35622799212907
2	26.01961263543221	12.74410024184465	36.258146535034584	24.978140587688557
3	25.28590284377027	14.386115797882812	23.288708705412205	37.03927265293472
4	27.269764731447584	22.27755365974418	22.416985796903365	28.03569581190487
5	29.54420483311978	26.042843169418912	24.150317515845092	20.262634481616217
6	25.210944184195323	29.049453319480328	24.3865717776713	21.353030718653056
7	20.365266580794657	23.256837577824307	38.60838408931356	17.769511752067483
8	22.5700472312488	22.160832949388983	30.38923272044471	24.879887098917507
9	21.576147691193576	20.186070242425902	33.5203987830806	24.71738328329992
10-14	25.181024110649325	24.593973996462204	26.061061057092523	24.163940835795945
15-19	25.217886051054748	24.013163050450252	26.552465997243512	24.216484901251484
20-24	24.953772812969707	23.983030115818107	26.588741170607435	24.474455900604752
25-29	24.96376626097043	24.219071684327876	26.36432142588437	24.452840628817327
30-34	24.992033553737702	24.077946266799355	26.14973755812858	24.780282621334354
35-39	25.19630641032916	23.977683078583336	26.235841503448537	24.590169007638966
40-44	25.08205982269664	23.95279495081335	26.284666657110257	24.680478569379755
45-49	25.201041193595202	23.833431757498094	26.267875753362613	24.69765129554409
50-54	25.00337540873407	24.076742909164924	26.195292002945003	24.724589679156004
55-59	25.241298747334074	23.967882194604396	26.00342387157025	24.78739518649129
60-64	25.03274581783811	23.8085266651168	26.263460462701133	24.895267054343957
65-69	25.232728373071073	24.06864877535598	25.990257581775154	24.708365269797785
70-74	25.262686680355138	23.964283106061707	25.921811266759896	24.851218946823256
75-79	25.226411615136623	23.95836439604319	25.92255827894026	24.892665709879925
80-84	25.183930839985123	23.986106798845256	26.024860631228837	24.80510172994078
85-89	25.42791893255838	23.871330992161592	25.8535205420405	24.847229533239528
90-94	25.357237826890415	23.974049446619873	25.85976305956383	24.808949666925884
95-99	25.48228040803374	24.137098372430806	25.685477067890815	24.695144151644634
100-104	25.534241510393624	24.28050897052981	25.44420354511514	24.741045973961423
105-109	25.551159032726584	24.35949583059834	25.225176074399002	24.864169062276076
110-114	25.7118754383531	24.65719181585621	25.010416680936725	24.620516064853966
115-119	25.530013230302583	25.035044557001456	24.692969706086803	24.74197250660915
120-124	25.647051466900617	25.059520022036114	24.50783410126305	24.785594409800222
125-129	25.219834760254006	25.22624234113277	24.53801687435977	25.015906024253454
130-134	25.09427267293282	25.27350303120002	24.455119815856115	25.177104480011042
135-139	24.64999282792295	25.270091152643744	24.59429691638701	25.485619103046297
140-144	24.405388366280377	25.405239924933497	24.68159584095923	25.507775867826894
145-149	23.923895919250164	25.253874549447673	25.032896722083343	25.789332809218816
150	23.663776010295624	25.051189247600636	25.333047720861067	25.951987021242672
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	86.0
1	63.0
2	38.0
3	40.0
4	39.5
5	42.0
6	62.5
7	71.0
8	72.0
9	84.5
10	109.5
11	144.0
12	175.5
13	208.5
14	268.0
15	355.5
16	493.5
17	709.0
18	1039.0
19	1625.0
20	2671.0
21	4487.0
22	6965.0
23	10429.0
24	16037.0
25	22637.0
26	30106.0
27	40756.5
28	54769.5
29	78761.0
30	113972.0
31	157676.0
32	216893.0
33	303199.0
34	409228.0
35	521347.0
36	663516.5
37	871904.5
38	1055224.0
39	1104058.5
40	1168400.5
41	1389384.5
42	1635945.0
43	1884675.5
44	2130549.0
45	2362975.5
46	2575898.5
47	2794240.0
48	3005620.5
49	3178597.0
50	3363671.0
51	3421608.0
52	3388903.0
53	3283986.0
54	3065261.5
55	2920330.0
56	2744348.5
57	2485094.0
58	2257905.5
59	1998085.0
60	1696503.0
61	1396776.5
62	1129385.0
63	891855.5
64	697962.5
65	537199.0
66	399687.5
67	302862.0
68	229609.0
69	169881.5
70	131642.0
71	109581.5
72	98257.0
73	78304.0
74	56374.0
75	41466.0
76	31930.0
77	23623.5
78	16356.0
79	11855.5
80	8927.0
81	6917.0
82	4951.5
83	3131.0
84	1812.0
85	863.5
86	415.5
87	177.5
88	40.0
89	19.5
90	12.5
91	5.5
92	6.5
93	9.0
94	9.5
95	7.5
96	7.0
97	9.0
98	14.0
99	16.5
100	66.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9000455476739879
2	0.003386071408374003
3	1.419218995764958E-4
4	0.004455113543227389
5	5.815712623949882E-4
6	0.005866619392276234
7	7.713146716113902E-6
8	0.0025052300533937952
9	5.738581156788743E-4
10-14	2.711942385385648E-4
15-19	0.0016246972242822321
20-24	0.0013019791656800265
25-29	8.465949835606619E-4
30-34	0.004838611197952573
35-39	0.004695455194901499
40-44	0.003527067730344565
45-49	0.002393543688944466
50-54	0.0017360750628629168
55-59	0.0019699376712954907
60-64	0.0062436380037598814
65-69	0.0025777336325252657
70-74	0.0030112124779708674
75-79	0.0039445032306206496
80-84	0.0023799685507241057
85-89	0.00269312230739833
90-94	0.0011347581448746772
95-99	0.0030269472972717397
100-104	0.004382918489964564
105-109	0.0014439010652565224
110-114	0.005175212920643784
115-119	0.004925306967041693
120-124	0.008783114428573222
125-129	0.0066197310376375955
130-134	0.006351313531916832
135-139	0.00978458939819345
140-144	0.004356076739392487
145-149	0.006087215388357091
150	0.007128490195032468
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	6.4824386E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.56935729973867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.27868993753748	19.22108931483841
2	15.3348867690315	8.76215718510992
3	5.734017151908645	4.914515543271239
4	3.020233085194553	3.45144472557661
5	1.758003376496072	2.511251329863165
6	1.151092743572715	1.9731587925759215
7	0.8483595116951231	1.6965960205774857
8	0.6155498224358085	1.4068690250367446
9	0.49245013605508814	1.266208550033646
>10	3.1690555904387194	17.44508075105296
>50	0.3247040046279279	6.456066851975095
>100	0.23091808182214396	12.9166710385348
>500	0.02427005844148318	4.812087634083265
>1k	0.016033779532988502	9.000236502722297
>5k	0.0013733059446236076	2.698379401004052
>10k+	3.6264526531235323E-4	1.4681873337440838
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.633306175857956E-5	1.5426293432227803E-6	0.0	1.5426293432227803E-6	0.0
2	1.1106931271204019E-4	6.170517372891121E-6	1.5426293432227803E-6	6.170517372891121E-6	0.0
3	1.8665815052995642E-4	6.170517372891121E-6	1.5426293432227803E-6	9.255776059336682E-6	2.1596810805118925E-5
4	3.054406099581105E-4	6.170517372891121E-6	1.5426293432227803E-6	1.2341034745782243E-5	3.2395216207678386E-5
5	3.7485893040313566E-4	6.170517372891121E-6	3.0852586864455607E-6	1.3883664089005023E-5	3.548047489412395E-5
6	4.5661828559394297E-4	6.170517372891121E-6	3.0852586864455607E-6	1.5426293432227803E-5	3.548047489412395E-5
7	5.075250539202948E-4	6.170517372891121E-6	3.0852586864455607E-6	1.6968922775450586E-5	3.548047489412395E-5
8	5.507186755305326E-4	6.170517372891121E-6	3.0852586864455607E-6	1.8511552118673364E-5	4.319362161023785E-5
9	6.67958505615464E-4	6.170517372891121E-6	3.0852586864455607E-6	1.8511552118673364E-5	4.6278880296683414E-5
10-11	8.237640692809647E-4	6.170517372891121E-6	3.0852586864455607E-6	1.8511552118673364E-5	4.936413898312897E-5
12-13	9.834262063045224E-4	6.9418320445025116E-6	3.0852586864455607E-6	2.0054181461896146E-5	6.247648840052261E-5
14-15	0.0011623712101183651	9.255776059336682E-6	3.0852586864455607E-6	2.0054181461896146E-5	8.407329920564154E-5
16-17	0.0013528859340063784	9.255776059336682E-6	3.856573358056951E-6	2.3139440148341707E-5	8.71585578920871E-5
18-19	0.0015511138046105056	1.0798405402559462E-5	4.627888029668341E-6	2.7767328178010046E-5	9.41003899365896E-5
20-21	0.0017092333122908406	1.2341034745782243E-5	4.627888029668341E-6	3.1623901536066996E-5	9.718564862303516E-5
22-23	0.0018526978412105593	1.7740237447061975E-5	5.399202701279731E-6	3.548047489412395E-5	1.0335616599592629E-4
24-25	0.0020285575863379563	2.1596810805118928E-5	7.713146716113902E-6	3.856573358056951E-5	1.1261194205526296E-4
26-27	0.002227556771613695	2.3910754819953096E-5	7.713146716113902E-6	4.319362161023785E-5	1.1878245942815409E-4
28-29	0.002460493802440335	2.6224698834787264E-5	9.255776059336682E-6	4.550756562507202E-5	1.3189480884554774E-4
30-31	0.0027296826228327097	2.7767328178010046E-5	9.255776059336682E-6	4.859282431151758E-5	1.3806532621843886E-4
32-33	0.0031461925455028607	2.7767328178010046E-5	9.255776059336682E-6	5.1678082997963146E-5	1.4577847293455274E-4
34-35	0.0036352060473044817	2.930995752123283E-5	9.255776059336682E-6	5.3220712341185925E-5	1.5349161965066665E-4
36-37	0.004224490456415583	2.930995752123283E-5	9.255776059336682E-6	5.553465635602009E-5	1.7354580111256278E-4
38-39	0.005065994763143611	2.930995752123283E-5	9.255776059336682E-6	5.784860037085426E-5	1.889720945447906E-4
40-41	0.006111897457848656	2.930995752123283E-5	9.255776059336682E-6	6.0162544385688435E-5	1.9899918527573868E-4
42-43	0.007468639965213091	3.0081272192844218E-5	9.255776059336682E-6	6.247648840052261E-5	2.1056890534990952E-4
44-45	0.009065261335448669	3.470916022251256E-5	1.0027090730948071E-5	6.556174708696817E-5	2.205959960808576E-4
46-47	0.011000489846521647	3.548047489412395E-5	1.0798405402559462E-5	6.787569110180234E-5	2.2599519878213734E-4
48-49	0.013430131062097526	3.625178956573534E-5	1.0798405402559462E-5	6.941832044502512E-5	2.360222895130854E-4
50-51	0.016601776991763566	3.702310423734673E-5	1.0798405402559462E-5	7.018963511663652E-5	2.537625269601474E-4
52-53	0.020453722461790842	3.702310423734673E-5	1.0798405402559462E-5	7.558883781791624E-5	2.66874876377541E-4
54-55	0.025120176225039755	3.702310423734673E-5	1.3883664089005023E-5	7.713146716113901E-5	2.6996013506398655E-4
56-57	0.03066978528728371	3.93370482521809E-5	1.3883664089005023E-5	8.330198453403014E-5	2.761306524368777E-4
58-59	0.03751983088586447	4.010836292379229E-5	1.3883664089005023E-5	9.564301927981238E-5	2.877003725110486E-4
60-61	0.04595647076394985	4.010836292379229E-5	1.3883664089005023E-5	1.0489879533914907E-4	2.9541351922716247E-4
62-63	0.056875972569952304	4.010836292379229E-5	1.4654978760616414E-5	1.0489879533914907E-4	3.0158403660005353E-4
64-65	0.07041794425943348	4.242230693862646E-5	1.5426293432227803E-5	1.0567011001076047E-4	3.100684979877789E-4
66-67	0.08752030447307282	4.319362161023785E-5	1.5426293432227803E-5	1.0644142468237185E-4	3.247234767483953E-4
68-69	0.10859647787485407	4.473625095346063E-5	1.6197608103839196E-5	1.0798405402559463E-4	3.4940554623995974E-4
70-71	0.13422803572717218	4.6278880296683414E-5	1.6968922775450586E-5	1.1184062738365158E-4	3.671457836870217E-4
72-73	0.1662399085430597	4.782150963990619E-5	1.6968922775450586E-5	1.180111447565427E-4	3.725449863883015E-4
74-75	0.2076070570109218	4.782150963990619E-5	1.6968922775450586E-5	1.2032508877137687E-4	3.75630245074747E-4
76-77	0.25932139179845065	5.013545365474036E-5	1.6968922775450586E-5	1.2032508877137687E-4	3.8797127982052924E-4
78-79	0.3234346099321327	5.090676832635175E-5	1.6968922775450586E-5	1.2109640344298825E-4	3.964557412082546E-4
80-81	0.4020894235697041	5.090676832635175E-5	1.7740237447061975E-5	1.257242914726566E-4	3.995409998947001E-4
82-83	0.49762985799819226	5.1678082997963146E-5	1.8511552118673364E-5	1.3035217950232495E-4	3.995409998947001E-4
84-85	0.6159865517276168	5.2449397669574535E-5	2.0825496133507536E-5	1.3266612351715913E-4	4.0416888792436847E-4
86-87	0.7653400681650884	5.2449397669574535E-5	2.1596810805118925E-5	1.334374381887705E-4	4.11110719968871E-4
88-89	0.9465874462736908	5.2449397669574535E-5	2.1596810805118925E-5	1.342087528603819E-4	4.195951813565963E-4
90-91	1.1623264738674115	5.2449397669574535E-5	2.1596810805118925E-5	1.3575138220360468E-4	4.203664960282077E-4
92-93	1.4175244174314277	5.3220712341185925E-5	2.1596810805118925E-5	1.3883664089005024E-4	4.242230693862646E-4
94-95	1.7203757240369388	5.3992027012797314E-5	2.1596810805118925E-5	1.411505849048844E-4	4.365641041320468E-4
96-97	2.078592769085387	5.553465635602009E-5	2.1596810805118925E-5	1.473211022777755E-4	4.489051388778291E-4
98-99	2.5009646832597845	5.553465635602009E-5	2.3910754819953096E-5	1.5349161965066665E-4	4.5276171223588603E-4
100-101	2.987939600384337	6.170517372891121E-5	2.6224698834787268E-5	1.5580556366550082E-4	4.6201748829522273E-4
102-103	3.5306574288262444	6.170517372891121E-5	2.6224698834787268E-5	1.573481930087236E-4	4.735872083693936E-4
104-105	4.1401533675922515	6.324780307213399E-5	2.6224698834787268E-5	1.5889082235194638E-4	4.7667246705583913E-4
106-107	4.823447922823364	6.324780307213399E-5	2.6224698834787268E-5	1.6043345169516914E-4	4.782150963990619E-4
108-109	5.588623700963399	6.324780307213399E-5	2.6224698834787268E-5	1.650613397248375E-4	4.8284298442873025E-4
110-111	6.421758472189771	6.401911774374538E-5	2.6224698834787268E-5	1.6660396906806028E-4	4.8824218713001E-4
112-113	7.314647762340549	7.09609497882479E-5	2.6224698834787268E-5	1.6968922775450584E-4	5.028971658906264E-4
114-115	8.263183549474729	7.09609497882479E-5	2.6224698834787268E-5	1.7200317176934002E-4	5.12152941949963E-4
116-117	9.269394391178654	7.09609497882479E-5	2.6224698834787268E-5	1.743171157841742E-4	5.136955712931858E-4
118-119	10.342061704988613	7.250357913147068E-5	2.6224698834787268E-5	1.743171157841742E-4	5.144668859647973E-4
120-121	11.474932442861858	7.404620847469346E-5	2.6224698834787268E-5	1.743171157841742E-4	5.152382006364086E-4
122-123	12.660544135350545	7.404620847469346E-5	2.776732817801005E-5	1.743171157841742E-4	5.214087180092997E-4
124-125	13.870052698995098	7.404620847469346E-5	2.930995752123283E-5	1.7817368914223113E-4	5.29893179397025E-4
126-127	15.100500913344556	7.404620847469346E-5	3.085258686445561E-5	1.8357289184351086E-4	5.37606326113139E-4
128-129	16.377471743426923	7.404620847469346E-5	3.1623901536066996E-5	1.8434420651512225E-4	5.399202701279731E-4
130-131	17.697823624584736	7.404620847469346E-5	3.2395216207678386E-5	1.8511552118673366E-4	5.422342141428074E-4
132-133	19.039739150016786	7.481752314630484E-5	3.2395216207678386E-5	1.8588683585834504E-4	5.484047315156984E-4
134-135	20.39437519701305	7.558883781791624E-5	3.2395216207678386E-5	1.9128603855962477E-4	5.561178782318123E-4
136-137	21.737606585274868	7.558883781791624E-5	3.2395216207678386E-5	1.9282866790284756E-4	5.700015423208174E-4
138-139	23.093898644871082	7.636015248952763E-5	3.3166530879289775E-5	1.9359998257445894E-4	5.730868010072629E-4
>>END_MODULE
