##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261776_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27561714
Sequences flagged as poor quality	0
Sequence length	150
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1199442458477	32.0	32.0	32.0	32.0	32.0
2	31.58371620139444	32.0	32.0	32.0	32.0	32.0
3	35.473931084256954	37.0	37.0	37.0	32.0	37.0
4	36.22428808309962	37.0	37.0	37.0	37.0	37.0
5	36.42046742811423	37.0	37.0	37.0	37.0	37.0
6	39.80601786957081	41.0	41.0	41.0	37.0	41.0
7	39.93244487625117	41.0	41.0	41.0	37.0	41.0
8	40.090484466967474	41.0	41.0	41.0	37.0	41.0
9	40.16864484552739	41.0	41.0	41.0	37.0	41.0
10-14	40.18289974999378	41.0	41.0	41.0	38.6	41.0
15-19	40.148014495760314	41.0	41.0	41.0	37.0	41.0
20-24	40.12480659947346	41.0	41.0	41.0	37.0	41.0
25-29	40.06473857903031	41.0	41.0	41.0	37.0	41.0
30-34	40.01532906117522	41.0	41.0	41.0	37.0	41.0
35-39	40.002044510003984	41.0	41.0	41.0	37.0	41.0
40-44	39.93923132646976	41.0	41.0	41.0	37.0	41.0
45-49	39.89658424000772	41.0	41.0	41.0	37.0	41.0
50-54	39.904773970152945	41.0	41.0	41.0	37.0	41.0
55-59	39.841304717115925	41.0	41.0	41.0	37.0	41.0
60-64	39.78099030415888	41.0	41.0	41.0	37.0	41.0
65-69	39.75681780893598	41.0	41.0	41.0	37.0	41.0
70-74	39.71751212569726	41.0	41.0	41.0	37.0	41.0
75-79	39.40632045597745	41.0	40.2	41.0	36.0	41.0
80-84	39.72082316070764	41.0	41.0	41.0	37.0	41.0
85-89	39.70961045456026	41.0	41.0	41.0	37.0	41.0
90-94	39.67601858868429	41.0	41.0	41.0	37.0	41.0
95-99	39.65053044233751	41.0	41.0	41.0	37.0	41.0
100-104	39.58103958266166	41.0	41.0	41.0	37.0	41.0
105-109	39.48152373252259	41.0	41.0	41.0	37.0	41.0
110-114	39.40648480714951	41.0	41.0	41.0	37.0	41.0
115-119	39.24185122158948	41.0	41.0	41.0	37.0	41.0
120-124	39.11032695571836	41.0	41.0	41.0	37.0	41.0
125-129	39.04095134286641	41.0	41.0	41.0	37.0	41.0
130-134	38.82836764070623	41.0	41.0	41.0	33.0	41.0
135-139	38.65049043756858	41.0	41.0	41.0	32.0	41.0
140-144	38.3967429601802	41.0	40.2	41.0	32.0	41.0
145-149	38.157098147089116	41.0	37.0	41.0	32.0	41.0
150	35.26913910361308	41.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	14.0
16	78.0
17	187.0
18	402.0
19	890.0
20	2252.0
21	4702.0
22	8827.0
23	15735.0
24	27135.0
25	42934.0
26	65048.0
27	93442.0
28	127487.0
29	167066.0
30	211446.0
31	262471.0
32	318802.0
33	384108.0
34	465594.0
35	570603.0
36	722852.0
37	962933.0
38	1424335.0
39	2845692.0
40	1.8836679E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.536894302462656	11.893361173390934	4.256793732243987	31.312950791902423
2	26.195726727435144	12.462647515588927	40.06723519913714	21.274390557838792
3	22.91227606526938	17.197286787026382	26.642403299011086	33.248033848693154
4	25.33752436441362	26.402715738215697	25.181227843812614	23.078532053558064
5	27.420696695423224	29.497751845186404	25.15692964523179	17.92462181415858
6	22.55774688935331	30.61258131143521	26.435749864938284	20.393921934273195
7	18.211040866326382	22.234966954522495	43.40206853608596	16.151923643065157
8	19.298868713317322	21.624943209264853	34.26099697573235	24.81519110168548
9	19.443656515701456	19.543276590127885	37.197614052594844	23.81545284157582
10-14	24.431609270310535	25.836841596471533	27.583943359993185	22.147605773224747
15-19	24.48484020986107	25.117312063702542	27.658631284467035	22.739216441969358
20-24	24.36505582713082	25.123646733834686	27.632958693493965	22.878338745540525
25-29	24.105239520086872	25.159524019305323	27.935748385283404	22.7994880753244
30-34	24.141965446273534	25.26637652810253	27.439339749424725	23.152318276199217
35-39	24.338009954270497	25.110895803798016	27.458445684541683	23.092648557389804
40-44	24.250554146092547	25.233324785122807	27.59305467328057	22.923066395504076
45-49	24.07605241132246	25.252178172128602	27.626020401880343	23.045749014668598
50-54	23.895121649986656	25.456881714702938	27.315681145736022	23.33231548957438
55-59	24.30376462317736	25.261258181118023	27.16094575302285	23.27403144268176
60-64	23.896197472787907	25.241841045330528	27.486136366317965	23.3758251155636
65-69	24.17439738305542	25.310577455040207	27.51972634220752	22.99529881969686
70-74	24.031284416531072	25.244548829294704	27.405130623818692	23.31903613035554
75-79	24.084185513022447	25.329472560465398	27.01967504263505	23.566666883877108
80-84	23.85827335485008	25.183440606802677	27.42460141022505	23.533684628122195
85-89	24.283599464299698	24.931096169689447	27.272549620630283	23.512754745380573
90-94	24.43418005343367	25.31280401271531	27.024638331177076	23.228377602673937
95-99	24.692512879171925	25.348069317356664	26.68893954440239	23.270478259069012
100-104	24.151229383661853	25.202266561326773	27.06091143194828	23.585592623063086
105-109	24.530381162046165	25.30198240151761	26.627227144633856	23.540409291802373
110-114	24.64816874635148	25.49738154320127	26.624007559381784	23.230442151065464
115-119	24.4706412101575	25.965943793034274	26.162768496537907	23.400646500270316
120-124	24.737793221332215	25.909931128501253	25.899013692170442	23.45326195799609
125-129	24.555406667341472	25.935173511547543	25.97762770852679	23.531792112584192
130-134	24.502095370389156	26.0774154580012	25.747154827577965	23.673334344031673
135-139	24.388851990971638	26.445599128413512	25.23652018087896	23.929028699735895
140-144	24.389963394353195	26.691311782491955	24.962678535460935	23.956046287693912
145-149	24.03511951828436	26.9366906102491	24.96152828729071	24.066661584175822
150	24.131324149984497	26.446509874509793	24.883583265771357	24.53858270973435
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	218.0
1	147.5
2	83.0
3	87.5
4	91.5
5	97.5
6	100.0
7	116.5
8	137.5
9	153.5
10	180.0
11	195.0
12	231.0
13	287.5
14	354.5
15	477.5
16	674.5
17	978.5
18	1547.5
19	2442.5
20	3785.5
21	6120.0
22	9399.0
23	13457.5
24	19703.0
25	29404.0
26	42114.0
27	58197.0
28	82285.0
29	114706.0
30	148750.0
31	187958.5
32	242054.5
33	314083.0
34	399824.5
35	493312.5
36	554900.5
37	681046.5
38	801294.5
39	742701.5
40	677209.5
41	726848.5
42	802797.5
43	894098.5
44	1001814.5
45	1079986.0
46	1137352.0
47	1179767.5
48	1227770.0
49	1288044.0
50	1325459.0
51	1300042.0
52	1254694.0
53	1199720.0
54	1101151.0
55	1040765.0
56	968494.0
57	843105.5
58	737607.5
59	637943.0
60	527210.0
61	418595.5
62	327426.5
63	247585.5
64	187281.5
65	137624.5
66	95838.5
67	71022.5
68	51806.5
69	35216.5
70	24429.0
71	17177.0
72	13590.5
73	9942.5
74	6191.0
75	4088.0
76	3293.0
77	2285.0
78	1094.5
79	491.0
80	333.0
81	299.0
82	268.5
83	175.5
84	91.0
85	49.0
86	25.0
87	6.5
88	4.5
89	3.5
90	1.5
91	2.0
92	1.0
93	0.0
94	0.5
95	1.0
96	0.5
97	0.5
98	1.0
99	1.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.5169303331425614
2	0.21354985397497414
3	0.0
4	0.0
5	0.0
6	0.007586610905257925
7	0.0
8	0.0
9	0.0
10-14	8.127215890854973E-5
15-19	4.789252221396681E-5
20-24	2.684883821086018E-5
25-29	5.776128436714785E-4
30-34	1.1828001698297863E-4
35-39	2.7574482486829375E-4
40-44	0.0014150063381399285
45-49	0.002843799917523272
50-54	6.603362911319666E-4
55-59	3.9257355329933404E-4
60-64	6.559824254761514E-4
65-69	6.073642589862154E-4
70-74	0.0010369456703599783
75-79	4.2377625716600936E-4
80-84	0.09123017530767498
85-89	6.668670896156894E-4
90-94	0.001330831602127502
95-99	4.37563498409424E-4
100-104	5.043227717985899E-4
105-109	8.119959448095283E-4
110-114	0.001880869963312151
115-119	0.08269732426655324
120-124	0.0010826612597460376
125-129	8.918168151661395E-4
130-134	0.0020767939178238334
135-139	0.0014048473182763596
140-144	8.294114074327888E-4
145-149	0.0015412684421585682
150	0.002173304606527736
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.7561714E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.36085976755052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.72074698204672	23.119460080630404
2	12.395415290447364	7.774617613685426
3	4.555043434767252	4.285502351785124
4	2.313638775726437	2.9023080479329626
5	1.4235126243609717	2.2321289894961125
6	0.9432139083064595	1.7747999465521278
7	0.6901892008226117	1.5151448718052982
8	0.503251694199281	1.2625924647652695
9	0.4019773999281605	1.1345721181984454
>10	2.545556764860887	15.414974601641692
>50	0.2676577080053213	5.832518798501563
>100	0.1984138781317345	12.345045046982932
>500	0.02259010975805395	4.884622544418224
>1k	0.01673475080878682	10.06335517074303
>5k	0.0016844847729140502	3.647225137200512
>10k+	3.729930568592794E-4	1.8111322156610463
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT	44405	0.16111117037206032	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.991043517830567E-5	0.0	0.0	7.256442759691941E-6	0.0
2	5.8051542077535525E-5	3.6282213798459703E-6	0.0	2.1769328279075823E-5	3.6282213798459703E-6
3	7.619264897676537E-5	3.6282213798459703E-6	0.0	2.1769328279075823E-5	3.6282213798459703E-6
4	1.4150063381399285E-4	3.6282213798459703E-6	0.0	2.1769328279075823E-5	1.0884664139537911E-5
5	1.5601351933337672E-4	3.6282213798459703E-6	0.0	2.1769328279075823E-5	1.4512885519383881E-5
6	1.8141106899229853E-4	3.6282213798459703E-6	0.0	2.1769328279075823E-5	1.8141106899229853E-5
7	1.9955217589152837E-4	3.6282213798459703E-6	0.0	2.1769328279075823E-5	1.8141106899229853E-5
8	2.104368400310663E-4	3.6282213798459703E-6	0.0	2.1769328279075823E-5	1.8141106899229853E-5
9	2.9025771038767765E-4	7.256442759691941E-6	0.0	2.5397549658921793E-5	1.8141106899229853E-5
10-11	3.501233631551361E-4	7.256442759691941E-6	1.8141106899229852E-6	3.0839881728690746E-5	2.3583438968998806E-5
12-13	4.517135617908233E-4	1.0884664139537911E-5	3.6282213798459703E-6	3.809632448838269E-5	3.0839881728690746E-5
14-15	5.623743138761254E-4	1.0884664139537911E-5	3.6282213798459703E-6	3.991043517830567E-5	3.6282213798459706E-5
16-17	6.186117452637379E-4	1.0884664139537911E-5	3.6282213798459703E-6	3.991043517830567E-5	3.6282213798459706E-5
18-19	6.821056194110424E-4	1.0884664139537911E-5	3.6282213798459703E-6	4.3538656558151646E-5	3.6282213798459706E-5
20-21	7.329007187288861E-4	1.0884664139537911E-5	3.6282213798459703E-6	4.89809886279206E-5	3.6282213798459706E-5
22-23	7.746252645971146E-4	1.6326996209306866E-5	3.6282213798459703E-6	5.8051542077535525E-5	3.809632448838269E-5
24-25	8.308626959847272E-4	2.1769328279075823E-5	3.6282213798459703E-6	6.893620621707344E-5	3.991043517830567E-5
26-27	8.979847915118776E-4	2.1769328279075823E-5	3.6282213798459703E-6	7.256442759691941E-5	3.991043517830567E-5
28-29	9.977608794576418E-4	2.1769328279075823E-5	3.6282213798459703E-6	7.800675966668837E-5	3.991043517830567E-5
30-31	0.001110235742232867	2.1769328279075823E-5	3.6282213798459703E-6	8.344909173645732E-5	3.991043517830567E-5
32-33	0.00123359526914763	2.1769328279075823E-5	3.6282213798459703E-6	8.52632024263803E-5	4.89809886279206E-5
34-35	0.0014004934526205446	2.1769328279075823E-5	3.6282213798459703E-6	8.707731311630329E-5	6.167976345738149E-5
36-37	0.0015601351933337672	2.1769328279075823E-5	3.6282213798459703E-6	8.889142380622628E-5	7.800675966668836E-5
38-39	0.0018376941288919839	2.1769328279075823E-5	3.6282213798459703E-6	9.614786656591821E-5	1.0340430932561016E-4
40-41	0.002202330377566504	2.1769328279075823E-5	7.256442759691941E-6	1.1610308415507105E-4	1.2335952691476298E-4
42-43	0.002668556824876711	2.1769328279075823E-5	7.256442759691941E-6	1.1973130553491702E-4	1.3061596967445493E-4
44-45	0.0032563286884117583	2.1769328279075823E-5	7.256442759691941E-6	1.1973130553491702E-4	1.3061596967445493E-4
46-47	0.0039039662047142642	2.1769328279075823E-5	7.256442759691941E-6	1.2517363760468597E-4	1.3061596967445493E-4
48-49	0.004865444870373446	2.1769328279075823E-5	7.256442759691941E-6	1.3243008036437792E-4	1.342441910543009E-4
50-51	0.006046430929513309	2.1769328279075823E-5	7.256442759691941E-6	1.3787241243414688E-4	1.4512885519383882E-4
52-53	0.007552142802149387	2.1769328279075823E-5	7.256442759691941E-6	1.3787241243414688E-4	1.487570765736848E-4
54-55	0.009349926495863066	2.1769328279075823E-5	1.0884664139537911E-5	1.4150063381399285E-4	1.5601351933337672E-4
56-57	0.011329121258569042	2.3583438968998806E-5	1.4512885519383881E-5	1.487570765736848E-4	1.5601351933337672E-4
58-59	0.01376365780444569	2.9025771038767763E-5	1.4512885519383881E-5	1.487570765736848E-4	1.5601351933337672E-4
60-61	0.016958306729400068	2.9025771038767763E-5	1.4512885519383881E-5	1.5057118726360778E-4	1.578276300232997E-4
62-63	0.02090399747998256	3.0839881728690746E-5	1.4512885519383881E-5	1.578276300232997E-4	1.6145585140314568E-4
64-65	0.025800282232084696	3.265399241861373E-5	1.4512885519383881E-5	1.6508407278299165E-4	1.6871229416283762E-4
66-67	0.03188843770746623	3.265399241861373E-5	1.4512885519383881E-5	1.6689818347291464E-4	1.723405155426836E-4
68-69	0.039498632051693155	3.265399241861373E-5	1.4512885519383881E-5	1.6689818347291464E-4	1.7778284761245256E-4
70-71	0.0492095665748509	3.265399241861373E-5	1.4512885519383881E-5	1.6689818347291464E-4	1.8141106899229853E-4
72-73	0.06126433210938913	3.265399241861373E-5	1.4512885519383881E-5	1.6871229416283762E-4	1.8141106899229853E-4
74-75	0.07662440732096705	3.265399241861373E-5	1.4512885519383881E-5	1.705264048527606E-4	1.8685340106206746E-4
76-77	0.09575601865689484	3.265399241861373E-5	1.4512885519383881E-5	1.705264048527606E-4	1.941098438217594E-4
78-79	0.11901473181239744	3.265399241861373E-5	1.4512885519383881E-5	1.705264048527606E-4	1.9773806520160538E-4
80-81	0.1480713427328939	3.265399241861373E-5	1.6326996209306866E-5	1.723405155426836E-4	1.9955217589152837E-4
82-83	0.1848814627421212	3.265399241861373E-5	1.8141106899229853E-5	1.7415462623260658E-4	1.9955217589152837E-4
84-85	0.23050453248299435	3.265399241861373E-5	1.8141106899229853E-5	1.7596873692252957E-4	2.0499450796129733E-4
86-87	0.28879735128228967	3.265399241861373E-5	1.8141106899229853E-5	1.850392903721445E-4	2.104368400310663E-4
88-89	0.3599467725410691	3.265399241861373E-5	1.8141106899229853E-5	1.850392903721445E-4	2.1769328279075824E-4
90-91	0.4446820687566818	3.265399241861373E-5	1.8141106899229853E-5	1.850392903721445E-4	2.2494972555045015E-4
92-93	0.5503667152195252	3.265399241861373E-5	1.8141106899229853E-5	1.8866751175199045E-4	2.2494972555045015E-4
94-95	0.6805110161146001	3.265399241861373E-5	1.8141106899229853E-5	1.8866751175199045E-4	2.5034727520937194E-4
96-97	0.8367168311811087	3.6282213798459706E-5	1.8141106899229853E-5	1.9229573313183642E-4	2.5034727520937194E-4
98-99	1.0225470738140596	3.6282213798459706E-5	1.8141106899229853E-5	1.9229573313183642E-4	2.5216138589929493E-4
100-101	1.2457752083197728	3.6282213798459706E-5	1.8141106899229853E-5	1.941098438217594E-4	2.557896072791409E-4
102-103	1.510252591692955	3.6282213798459706E-5	1.8141106899229853E-5	1.9773806520160538E-4	2.594178286589869E-4
104-105	1.8217843781413594	3.6282213798459706E-5	1.8141106899229853E-5	1.9955217589152837E-4	2.6486016072875583E-4
106-107	2.1854373788219412	3.991043517830567E-5	1.8141106899229853E-5	2.0136628658145136E-4	2.7393071417837077E-4
108-109	2.6040270935254606	3.991043517830567E-5	1.995521758915284E-5	2.0680861865122032E-4	2.7574482486829375E-4
110-111	3.074821471552894	4.3538656558151646E-5	2.1769328279075823E-5	2.104368400310663E-4	2.7937304624813973E-4
112-113	3.6064193975744763	4.3538656558151646E-5	2.1769328279075823E-5	2.1950739348068122E-4	2.811871569380627E-4
114-115	4.213132390823009	4.3538656558151646E-5	2.1769328279075823E-5	2.2132150417060418E-4	2.830012676279857E-4
116-117	4.893022981081655	4.716687793799761E-5	2.1769328279075823E-5	2.2132150417060418E-4	2.866294890078317E-4
118-119	5.645331055971337	5.0795099317843586E-5	2.3583438968998806E-5	2.2132150417060418E-4	2.866294890078317E-4
120-121	6.462676813205448	5.0795099317843586E-5	2.5397549658921793E-5	2.2857794693029613E-4	2.866294890078317E-4
122-123	7.342413465287391	5.623743138761254E-5	2.5397549658921793E-5	2.2857794693029613E-4	2.9025771038767765E-4
124-125	8.290132101363508	6.167976345738149E-5	2.5397549658921793E-5	2.2857794693029613E-4	2.9207182107760063E-4
126-127	9.30629350554904	6.167976345738149E-5	2.5397549658921793E-5	2.3039205762021911E-4	2.957000424574466E-4
128-129	10.387303924567245	6.167976345738149E-5	2.5397549658921793E-5	2.322061683101421E-4	2.993282638372926E-4
130-131	11.524557217305137	6.167976345738149E-5	2.5397549658921793E-5	2.322061683101421E-4	3.0114237452721557E-4
132-133	12.700197092241796	6.167976345738149E-5	2.5397549658921793E-5	2.3583438968998807E-4	3.047705959070615E-4
134-135	13.934987860334086	6.893620621707344E-5	2.5397549658921793E-5	2.3583438968998807E-4	3.047705959070615E-4
136-137	15.198113223292282	6.893620621707344E-5	2.5397549658921793E-5	2.3764850037991106E-4	3.2291170280629135E-4
138-139	16.49300366443103	7.075031690699643E-5	2.721166034884478E-5	2.4127672175975703E-4	3.301681455659833E-4
>>END_MODULE
