##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261774_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23368337
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.86615029559014	32.0	32.0	32.0	32.0	32.0
2	31.37520543288981	32.0	32.0	32.0	32.0	32.0
3	34.9399374889193	37.0	32.0	37.0	32.0	37.0
4	35.70197652490205	37.0	37.0	37.0	32.0	37.0
5	35.94900886614225	37.0	37.0	37.0	37.0	37.0
6	39.19534051567298	41.0	41.0	41.0	37.0	41.0
7	39.39828670735106	41.0	41.0	41.0	37.0	41.0
8	39.495760823716296	41.0	41.0	41.0	37.0	41.0
9	39.47606682495207	41.0	41.0	41.0	37.0	41.0
10-14	39.52004800341591	41.0	41.0	41.0	37.0	41.0
15-19	39.446631174481944	41.0	41.0	41.0	37.0	41.0
20-24	39.40904031810223	41.0	41.0	41.0	37.0	41.0
25-29	39.33503476092458	41.0	41.0	41.0	37.0	41.0
30-34	39.32869515704092	41.0	41.0	41.0	37.0	41.0
35-39	39.21864267876657	41.0	41.0	41.0	37.0	41.0
40-44	39.09611737454831	41.0	41.0	41.0	37.0	41.0
45-49	39.03015730216489	41.0	41.0	41.0	37.0	41.0
50-54	38.98258128509529	41.0	41.0	41.0	37.0	41.0
55-59	38.87400801349279	41.0	41.0	41.0	32.0	41.0
60-64	38.83302413860259	41.0	41.0	41.0	32.0	41.0
65-69	38.72165561460364	41.0	41.0	41.0	32.0	41.0
70-74	38.634654652575406	41.0	41.0	41.0	32.0	41.0
75-79	38.06890394468378	41.0	39.4	41.0	31.0	41.0
80-84	38.74342913661336	41.0	41.0	41.0	32.0	41.0
85-89	38.69084646459866	41.0	41.0	41.0	32.0	41.0
90-94	38.61255883120823	41.0	41.0	41.0	32.0	41.0
95-99	38.47129709743574	41.0	41.0	41.0	32.0	41.0
100-104	38.26118888990688	41.0	37.8	41.0	32.0	41.0
105-109	38.105427784613	41.0	37.0	41.0	32.0	41.0
110-114	37.88479644914399	41.0	37.0	41.0	30.0	41.0
115-119	37.53441802897656	41.0	37.0	41.0	27.0	41.0
120-124	37.20559844716379	41.0	37.0	41.0	27.0	41.0
125-129	36.8744546605948	41.0	37.0	41.0	27.0	41.0
130-134	36.46582868091982	41.0	37.0	41.0	25.0	41.0
135-139	35.979951050859974	41.0	33.0	41.0	22.0	41.0
140-144	35.47990673020506	41.0	32.0	41.0	22.0	41.0
145-149	34.797138700969604	41.0	32.0	41.0	22.0	41.0
150	29.829413749039993	32.0	22.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	19.0
13	176.0
14	1140.0
15	4808.0
16	15316.0
17	29253.0
18	41108.0
19	50319.0
20	61021.0
21	73841.0
22	88298.0
23	104025.0
24	121510.0
25	141112.0
26	163312.0
27	186715.0
28	215836.0
29	251441.0
30	289412.0
31	337248.0
32	398370.0
33	474813.0
34	574646.0
35	709634.0
36	909562.0
37	1243500.0
38	1934566.0
39	4043766.0
40	1.0903566E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.5814574779554	23.438840546048763	7.2281373699667615	30.75156460602908
2	27.151012610476883	26.34733542899416	28.963694022332874	17.537957938196083
3	21.269878981974628	26.81248135029891	27.818787447305297	24.098852220421165
4	23.61762841746077	34.899950304550984	21.13040821004935	20.352013067938895
5	26.04707148122835	36.37727297216603	18.708151278955583	18.86750426765003
6	22.034520471011696	36.76679260488241	19.17740659080704	22.021280333298858
7	21.00200079210056	20.57449757138373	34.25148101622522	24.172020620290485
8	20.210124494524365	24.049105419867917	26.573692428348668	29.16707765725905
9	21.102699777053026	22.58917697053068	29.228083282092342	27.08003997032395
10-14	24.35530109502311	27.361874431181256	23.69193362915574	24.590890844639897
15-19	24.217488963576002	26.80709078446989	24.440467071999194	24.534953179954915
20-24	24.127466344385823	26.945525994505882	24.572081730265257	24.35492593084304
25-29	23.83794214834117	26.86692282133661	24.760462872290816	24.534672158031402
30-34	23.827030661137538	27.07127176269345	24.625259805423845	24.476437770745168
35-39	23.555610110010523	27.28769798590588	24.658271775632794	24.498420128450807
40-44	23.614488902218277	26.937379204137635	24.900423637261685	24.547708256382403
45-49	23.586762817698347	26.93927346505744	24.959877159747958	24.514086557496253
50-54	23.727267834092277	27.014750536429013	24.75412941150805	24.503852217970664
55-59	23.783982143166174	26.89054216122034	24.80607718316146	24.519398512452028
60-64	23.73576240780115	26.65927543886271	25.20669548013068	24.398266673205455
65-69	24.060828425931984	26.554835860700337	25.269139400182116	24.115196313185564
70-74	24.041973205351336	26.67808428952651	25.123542873646848	24.156399631475306
75-79	23.98172520585898	26.73871445567397	25.02021975955869	24.25934057890836
80-84	23.798803710999977	26.986998857315243	24.916564105361232	24.297633326323552
85-89	23.88593811110249	26.857751779692208	24.779963199254876	24.47634690995043
90-94	23.78736269156449	27.103442064029586	24.874133880623397	24.235061363782524
95-99	23.691528726773527	27.033841187216684	24.979590701408362	24.295039384601427
100-104	24.114298794775728	27.298393453957637	24.585022328599653	24.002285422666983
105-109	24.013313977134075	27.57884467386139	24.34979996768261	24.058041381321917
110-114	24.106983185555933	27.557174110622736	24.31621726099131	24.019625442830023
115-119	24.358284415467473	27.536250167202862	24.273796526868825	23.831668890460836
120-124	24.657031176375924	27.732216369000756	24.074612870050714	23.536139584572602
125-129	25.190805244652715	27.74686320627408	23.846560504805478	23.21577104426773
130-134	25.806816762448225	27.691241722291586	23.512197502501127	22.989744012759058
135-139	25.969029796683017	27.613067416192795	23.734286487034655	22.683616300089533
140-144	26.29006947748546	27.669147718515706	23.505821194874716	22.534961609124117
145-149	26.897098134961972	27.082587058681334	23.699538446441082	22.320776359915612
150	26.510765642941948	27.042225397803133	23.97901086802697	22.467998091227955
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	166.0
1	128.5
2	93.0
3	102.5
4	125.5
5	155.0
6	188.0
7	213.0
8	261.5
9	293.0
10	326.0
11	371.5
12	414.5
13	477.0
14	555.5
15	711.0
16	911.5
17	1170.0
18	1597.5
19	2322.5
20	3592.5
21	5413.0
22	8122.5
23	11596.5
24	16668.0
25	23574.0
26	33162.5
27	46607.5
28	63455.0
29	88671.5
30	113744.5
31	140250.0
32	178915.5
33	240575.5
34	327071.5
35	376076.0
36	406772.5
37	503928.5
38	565717.5
39	545286.0
40	531287.0
41	565441.0
42	626153.5
43	702570.0
44	765068.0
45	836428.5
46	925007.5
47	982088.0
48	1045634.0
49	1131227.5
50	1214672.0
51	1181590.5
52	1089481.0
53	1039023.5
54	974451.0
55	928249.0
56	876400.0
57	775630.0
58	674337.5
59	570313.5
60	474480.5
61	392787.5
62	315607.0
63	254595.0
64	195739.5
65	145208.0
66	109039.0
67	82973.5
68	66342.0
69	51682.0
70	37852.0
71	27867.0
72	22042.5
73	15920.0
74	10432.5
75	7021.5
76	4605.5
77	3048.0
78	1963.5
79	1317.0
80	855.0
81	582.0
82	421.0
83	259.0
84	182.5
85	143.0
86	101.5
87	88.5
88	71.0
89	51.5
90	46.5
91	35.5
92	33.5
93	31.5
94	22.5
95	21.0
96	14.0
97	12.0
98	14.5
99	17.5
100	33.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.540499009407473
2	0.02608658031592064
3	0.0
4	0.0
5	3.851365204122142E-4
6	0.0
7	3.936951097547078E-4
8	0.0
9	0.0
10-14	7.386062602572019E-4
15-19	1.4549601882239203E-4
20-24	9.833819154525202E-4
25-29	2.559018213405601E-4
30-34	0.002399828451635219
35-39	1.9171240127185773E-4
40-44	3.8684823828071294E-4
45-49	4.322087617959293E-4
50-54	3.0810921632977134E-5
55-59	0.0018477994390443786
60-64	4.0653299376844837E-4
65-69	5.597317429990846E-4
70-74	5.717137680785757E-4
75-79	4.561728119549114E-4
80-84	1.5918976177038186E-4
85-89	9.551385706222912E-4
90-94	0.0010843732696939454
95-99	2.8671274297353724E-4
100-104	2.3792878372132342E-4
105-109	4.8356029785089115E-4
110-114	2.53334244537812E-4
115-119	7.360386834544537E-5
120-124	2.0027099061435138E-4
125-129	1.4036086521689584E-4
130-134	4.031095580314508E-4
135-139	7.942370909834106E-4
140-144	3.26082253949008E-4
145-149	1.7716279938961854E-4
150	0.0018572138873211217
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3368337E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.212285625061313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.3336152210995	21.130302281320414
2	12.437833858833725	7.266751104826187
3	4.868547957252954	4.266642405197462
4	2.4948627601660984	2.915225741812036
5	1.5127947833538058	2.209609665171706
6	1.072851541206606	1.8804267393008767
7	0.7477176895336092	1.5289779899496698
8	0.5679952352523976	1.3273951236693549
9	0.45013512652921317	1.1834528297440043
>10	2.930250190204552	16.455977344639923
>50	0.30101295294224806	6.123872472905243
>100	0.23261055482896525	13.728103448302944
>500	0.02837649866142286	5.72765295234207
>1k	0.020071286542406634	11.030995373442645
>5k	0.001096008490424238	2.1744108639591677
>10k+	2.283351021717149E-4	1.0502036634165908
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1396473356234123E-5	0.0	0.0	4.279294671246824E-6	0.0
2	2.995506269872777E-5	8.558589342493648E-6	0.0	1.2837884013740473E-5	0.0
3	3.851365204122142E-5	8.558589342493648E-6	0.0	1.2837884013740473E-5	0.0
4	5.991012539745554E-5	8.558589342493648E-6	0.0	1.2837884013740473E-5	0.0
5	7.702730408244284E-5	8.558589342493648E-6	0.0	1.2837884013740473E-5	0.0
6	9.842377743867696E-5	8.558589342493648E-6	0.0	1.2837884013740473E-5	0.0
7	1.0270307210992378E-4	8.558589342493648E-6	0.0	1.2837884013740473E-5	0.0
8	1.1554095612366426E-4	8.558589342493648E-6	0.0	1.2837884013740473E-5	0.0
9	1.6689249217862614E-4	8.558589342493648E-6	0.0	1.2837884013740473E-5	0.0
10-11	2.1610438089796465E-4	1.2837884013740473E-5	0.0	1.4977531349363884E-5	0.0
12-13	3.2736604235038204E-4	1.7117178684987297E-5	0.0	1.7117178684987297E-5	0.0
14-15	4.2792946712468243E-4	1.7117178684987297E-5	0.0	1.7117178684987297E-5	0.0
16-17	5.113757132139955E-4	1.7117178684987297E-5	0.0	1.7117178684987297E-5	0.0
18-19	6.140787853239193E-4	1.925682602061071E-5	0.0	1.7117178684987297E-5	0.0
20-21	7.274800941119602E-4	2.1396473356234123E-5	0.0	3.637400470559801E-5	0.0
22-23	8.751157602699755E-4	2.1396473356234123E-5	0.0	4.2792946712468246E-5	0.0
24-25	0.0010398686051129782	2.1396473356234123E-5	0.0	4.2792946712468246E-5	0.0
26-27	0.0012110403919628513	2.1396473356234123E-5	0.0	4.493259404809165E-5	0.0
28-29	0.001463518777566414	2.1396473356234123E-5	0.0	4.7072241383715065E-5	0.0
30-31	0.0017052989264918594	2.1396473356234123E-5	0.0	4.7072241383715065E-5	0.0
32-33	0.002043363205520359	2.1396473356234123E-5	0.0	4.7072241383715065E-5	0.0
34-35	0.0024327790206038194	2.1396473356234123E-5	0.0	4.7072241383715065E-5	0.0
36-37	0.002886384255755983	2.1396473356234123E-5	0.0	4.7072241383715065E-5	2.139647335623412E-6
38-39	0.003363525611600004	2.1396473356234123E-5	0.0	4.7072241383715065E-5	4.279294671246824E-6
40-41	0.003954068276232066	2.1396473356234123E-5	0.0	4.7072241383715065E-5	4.279294671246824E-6
42-43	0.004722201669720871	2.1396473356234123E-5	0.0	5.135153605496189E-5	4.279294671246824E-6
44-45	0.005558803777949624	2.1396473356234123E-5	0.0	5.135153605496189E-5	4.279294671246824E-6
46-47	0.006495969310952679	2.1396473356234123E-5	0.0	5.991012539745554E-5	4.279294671246824E-6
48-49	0.0077369647656142584	2.1396473356234123E-5	0.0	6.418942006870236E-5	4.279294671246824E-6
50-51	0.00914913200712571	2.1396473356234123E-5	0.0	6.418942006870236E-5	4.279294671246824E-6
52-53	0.010976390831748103	2.1396473356234123E-5	0.0	6.846871473994919E-5	6.418942006870236E-6
54-55	0.012991938621905359	2.1396473356234123E-5	0.0	6.846871473994919E-5	8.558589342493648E-6
56-57	0.015379785048461086	2.1396473356234123E-5	0.0	7.06083620755726E-5	8.558589342493648E-6
58-59	0.018270448598888316	2.1396473356234123E-5	0.0	7.274800941119601E-5	8.558589342493648E-6
60-61	0.02185863718072878	2.1396473356234123E-5	0.0	7.274800941119601E-5	8.558589342493648E-6
62-63	0.026285567518133615	2.1396473356234123E-5	0.0	7.274800941119601E-5	8.558589342493648E-6
64-65	0.03187218671144634	2.1396473356234123E-5	0.0	7.274800941119601E-5	8.558589342493648E-6
66-67	0.03828257012897409	2.1396473356234123E-5	0.0	7.702730408244284E-5	8.558589342493648E-6
68-69	0.04640467141500056	2.1396473356234123E-5	0.0	7.702730408244284E-5	8.558589342493648E-6
70-71	0.05654446013851991	2.1396473356234123E-5	0.0	7.702730408244284E-5	8.558589342493648E-6
72-73	0.06921545166008175	2.1396473356234123E-5	0.0	8.558589342493649E-5	8.558589342493648E-6
74-75	0.08547249211614844	2.1396473356234123E-5	0.0	8.558589342493649E-5	8.558589342493648E-6
76-77	0.10546321717287799	2.567576802748095E-5	0.0	8.558589342493649E-5	8.558589342493648E-6
78-79	0.12988515186168362	2.995506269872777E-5	0.0	8.77255407605599E-5	8.558589342493648E-6
80-81	0.15948717275003352	2.995506269872777E-5	0.0	8.98651880961833E-5	8.558589342493648E-6
82-83	0.19750870590406155	2.995506269872777E-5	0.0	9.414448276743013E-5	8.558589342493648E-6
84-85	0.24496608380818885	2.995506269872777E-5	0.0	9.414448276743013E-5	8.558589342493648E-6
86-87	0.30403532780274434	2.995506269872777E-5	0.0	9.414448276743013E-5	8.558589342493648E-6
88-89	0.37656937248037803	3.209471003435118E-5	0.0	1.048427194455472E-4	8.558589342493648E-6
90-91	0.46477633389145323	3.4234357369974594E-5	0.0	1.1126166145241743E-4	1.0698236678117061E-5
92-93	0.5727835917463875	3.4234357369974594E-5	0.0	1.1768060345928767E-4	1.2837884013740473E-5
94-95	0.7035973505517317	3.4234357369974594E-5	0.0	1.240995454661579E-4	1.2837884013740473E-5
96-97	0.8613792243752733	3.4234357369974594E-5	0.0	1.2837884013740472E-4	1.2837884013740473E-5
98-99	1.0503935303569099	3.851365204122142E-5	0.0	1.2837884013740472E-4	1.2837884013740473E-5
100-101	1.2767639391711958	4.2792946712468246E-5	0.0	1.3479778214427497E-4	1.2837884013740473E-5
102-103	1.5417507030988127	4.2792946712468246E-5	0.0	1.4121672415114521E-4	1.2837884013740473E-5
104-105	1.854049348911735	4.2792946712468246E-5	0.0	1.4121672415114521E-4	1.2837884013740473E-5
106-107	2.2142803743372923	4.2792946712468246E-5	0.0	1.4121672415114521E-4	1.2837884013740473E-5
108-109	2.6317212902227487	4.2792946712468246E-5	0.0	1.4121672415114521E-4	1.2837884013740473E-5
110-111	3.0998140774844183	5.135153605496189E-5	0.0	1.4335637148676862E-4	1.2837884013740473E-5
112-113	3.6243678786385187	5.135153605496189E-5	0.0	1.4977531349363884E-4	1.2837884013740473E-5
114-115	4.21540908109978	5.135153605496189E-5	0.0	1.4977531349363884E-4	1.2837884013740473E-5
116-117	4.875073052909157	5.563083072620872E-5	0.0	1.5405460816488568E-4	1.2837884013740473E-5
118-119	5.60506937228781	5.563083072620872E-5	0.0	1.5619425550050909E-4	1.2837884013740473E-5
120-121	6.392397542024493	5.563083072620872E-5	0.0	1.6261319750737933E-4	1.2837884013740473E-5
122-123	7.241559380113356	5.563083072620872E-5	0.0	1.6689249217862614E-4	1.2837884013740473E-5
124-125	8.152499255723674	5.563083072620872E-5	0.0	1.6903213951424955E-4	1.2837884013740473E-5
126-127	9.117441690437794	5.563083072620872E-5	0.0	1.733114341854964E-4	1.2837884013740473E-5
128-129	10.146113521043453	5.563083072620872E-5	0.0	1.8400967086361342E-4	1.2837884013740473E-5
130-131	11.226455695157084	5.563083072620872E-5	0.0	1.968475548773539E-4	1.2837884013740473E-5
132-133	12.338729966107557	5.563083072620872E-5	0.0	1.9898720221297734E-4	1.2837884013740473E-5
134-135	13.4996833535908	5.563083072620872E-5	0.0	2.0112684954860075E-4	1.2837884013740473E-5
136-137	14.683907545496284	5.563083072620872E-5	0.0	2.0112684954860075E-4	1.2837884013740473E-5
138-139	15.892893876016936	6.204977273307896E-5	0.0	2.096854388910944E-4	1.4977531349363884E-5
>>END_MODULE
