##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261774_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23368337
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.927854172934943	32.0	32.0	32.0	32.0	32.0
2	31.495892454820385	32.0	32.0	32.0	32.0	32.0
3	35.32790754429808	37.0	32.0	37.0	32.0	37.0
4	36.18657604946385	37.0	37.0	37.0	32.0	37.0
5	36.37742125167058	37.0	37.0	37.0	37.0	37.0
6	39.79651774107845	41.0	41.0	41.0	37.0	41.0
7	39.87895390245357	41.0	41.0	41.0	37.0	41.0
8	39.99472786617208	41.0	41.0	41.0	37.0	41.0
9	40.0885508883238	41.0	41.0	41.0	37.0	41.0
10-14	40.149301296022905	41.0	41.0	41.0	37.0	41.0
15-19	40.12633897739492	41.0	41.0	41.0	37.0	41.0
20-24	40.11451493531611	41.0	41.0	41.0	37.0	41.0
25-29	40.055995914471794	41.0	41.0	41.0	37.0	41.0
30-34	40.00812201570013	41.0	41.0	41.0	37.0	41.0
35-39	39.99453108708592	41.0	41.0	41.0	37.0	41.0
40-44	39.933263663563224	41.0	41.0	41.0	37.0	41.0
45-49	39.88920971141421	41.0	41.0	41.0	37.0	41.0
50-54	39.897429568907704	41.0	41.0	41.0	37.0	41.0
55-59	39.83449037901156	41.0	41.0	41.0	37.0	41.0
60-64	39.77448812039984	41.0	41.0	41.0	37.0	41.0
65-69	39.75311891470925	41.0	41.0	41.0	37.0	41.0
70-74	39.71187689564731	41.0	41.0	41.0	37.0	41.0
75-79	39.39801201942612	41.0	40.2	41.0	36.0	41.0
80-84	39.718165884033596	41.0	41.0	41.0	37.0	41.0
85-89	39.708697311237856	41.0	41.0	41.0	37.0	41.0
90-94	39.673868850830075	41.0	41.0	41.0	37.0	41.0
95-99	39.65171011527265	41.0	41.0	41.0	37.0	41.0
100-104	39.579226985643004	41.0	41.0	41.0	37.0	41.0
105-109	39.479425437933386	41.0	41.0	41.0	37.0	41.0
110-114	39.40451491263585	41.0	41.0	41.0	37.0	41.0
115-119	39.23731851350826	41.0	41.0	41.0	37.0	41.0
120-124	39.104729061379075	41.0	41.0	41.0	37.0	41.0
125-129	39.03180723557692	41.0	41.0	41.0	37.0	41.0
130-134	38.81723725569346	41.0	41.0	41.0	33.0	41.0
135-139	38.633733876741	41.0	41.0	41.0	32.0	41.0
140-144	38.37483696850144	41.0	39.4	41.0	32.0	41.0
145-149	38.123622720778116	41.0	37.0	41.0	32.0	41.0
150	35.19298454999173	41.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	12.0
16	52.0
17	121.0
18	263.0
19	688.0
20	1757.0
21	3746.0
22	6889.0
23	12256.0
24	21078.0
25	34340.0
26	52647.0
27	76135.0
28	104984.0
29	139342.0
30	177747.0
31	221845.0
32	271534.0
33	331695.0
34	402208.0
35	496634.0
36	630504.0
37	842207.0
38	1248414.0
39	2497672.0
40	1.5793565E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.60246326967947	11.382839389204342	4.970462397387294	32.0442349437289
2	26.74165374248162	12.321059270709304	38.603669117705394	22.333617869103684
3	23.939264484246355	16.596234468888394	25.5291636713387	33.93533737552655
4	26.15838260120949	25.550234918299918	24.40824094585764	23.88314153463295
5	27.8496839548317	28.918938476452134	24.847763022246728	18.38361454646944
6	22.807205020522623	30.2119888725175	26.23591202693103	20.744894080028843
7	18.418580663228195	22.326205754393218	42.81459566421008	16.440617918168503
8	19.701350592470487	21.62488070931192	33.89176131788925	24.78200738032835
9	19.838998384865814	19.81740078466003	36.468624190073946	23.874976640400213
10-14	24.606286317426886	25.688338114134897	27.301485701577917	22.4038898668603
15-19	24.691910508360895	24.90266161067145	27.485176322317134	22.920251558650516
20-24	24.52595546016644	24.950837311808773	27.42006536743941	23.103141860585374
25-29	24.302243778730386	25.019724664852728	27.612162618707664	23.065868937709226
30-34	24.33718056633107	25.087500978889786	27.172144669426245	23.403173785352898
35-39	24.51508346823344	24.948755961895934	27.22311596462682	23.313044605243807
40-44	24.44150782140633	24.981408578318057	27.381306517534632	23.19577708274098
45-49	24.29279057682777	24.96529524194459	27.425853472922775	23.316060708304867
50-54	24.122518715082066	25.202915844769286	27.146293133163745	23.528272306984903
55-59	24.546767966958523	25.017626479468863	26.965095274224026	23.470510279348588
60-64	24.093882654940412	24.944405414659148	27.319735847782496	23.64197608261794
65-69	24.3762003537095	25.12132355228723	27.225663891281528	23.276812202721747
70-74	24.231651290461954	25.028321276779465	27.15734747033696	23.582679962421622
75-79	24.301572545027835	25.05566506645817	26.874729231931333	23.76803315658266
80-84	24.08907491431498	24.96147272049139	27.21480640664719	23.734645958546437
85-89	24.49173902713569	24.740472005707588	27.057903324313664	23.709885642843055
90-94	24.574901795866726	25.025318319974126	26.891626498447074	23.508153385712067
95-99	24.81191892249203	25.108386180758636	26.578503326817852	23.501191569931482
100-104	24.39822075625408	24.988794610992286	26.863776070855614	23.749208561898023
105-109	24.697441903588764	25.079922807145234	26.466038004203085	23.756597285062917
110-114	24.845065930037023	25.26416207897161	26.443772196735083	23.44699979425629
115-119	24.679588652964743	25.697622567167787	26.013284441510365	23.609504338357105
120-124	24.93431152487856	25.664069545193964	25.777691095513955	23.62392783441353
125-129	24.77292682950209	25.689241541341673	25.818721194125832	23.71911043503041
130-134	24.761954687979223	25.783721186895708	25.58824691136976	23.866077213755307
135-139	24.658810430774935	26.064726638810836	25.1698135892946	24.106649341119628
140-144	24.72892159054851	26.221705469813312	24.957133775801395	24.092239163836783
145-149	24.46987501485043	26.31690106989949	24.998909406408032	24.21431450884205
150	24.558347958461113	25.803689482065707	24.977153475622334	24.660809083850843
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	193.0
1	132.0
2	65.0
3	57.0
4	60.0
5	66.5
6	84.5
7	98.5
8	100.0
9	117.5
10	134.5
11	158.0
12	188.5
13	219.5
14	275.0
15	366.0
16	536.0
17	807.5
18	1227.5
19	1866.5
20	3057.5
21	5075.0
22	7660.0
23	10686.5
24	15776.5
25	23790.0
26	33744.0
27	46524.0
28	65393.5
29	91971.5
30	120507.5
31	151649.0
32	193115.0
33	247883.0
34	313436.0
35	384944.5
36	434363.5
37	522002.0
38	606904.0
39	575131.5
40	538464.5
41	584513.0
42	649763.0
43	724524.0
44	812105.0
45	886109.5
46	942254.0
47	984551.0
48	1035068.5
49	1093551.0
50	1139975.5
51	1133840.0
52	1104814.0
53	1063348.0
54	978223.0
55	922017.0
56	857445.0
57	754323.0
58	667940.5
59	581419.5
60	484526.5
61	389443.0
62	308293.0
63	235983.5
64	180726.0
65	134216.5
66	93240.5
67	68500.5
68	49869.0
69	33560.5
70	23319.5
71	16221.0
72	12375.0
73	8858.0
74	5392.5
75	3338.5
76	2356.5
77	1559.0
78	786.0
79	413.0
80	287.5
81	212.0
82	155.5
83	82.0
84	41.0
85	29.0
86	14.5
87	4.0
88	2.0
89	1.0
90	2.5
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.5
97	0.5
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.4914497338856418
2	0.21247553901674732
3	0.0
4	0.0
5	0.0
6	0.007214890815722146
7	0.0
8	0.0
9	0.0
10-14	4.364880564671761E-5
15-19	5.648668966045808E-5
20-24	2.3108191224732853E-5
25-29	5.67434473407329E-4
30-34	1.1554095612366426E-4
35-39	2.464873730638171E-4
40-44	0.0012906352728480422
45-49	0.002772127088033693
50-54	6.504527900295173E-4
55-59	3.671634827929775E-4
60-64	6.162184326595427E-4
65-69	5.169387962866164E-4
70-74	9.277510847263115E-4
75-79	3.8684823828071294E-4
80-84	0.09146222086749262
85-89	6.204977273307896E-4
90-94	0.0011887880596723678
95-99	3.8684823828071294E-4
100-104	4.989657586673797E-4
105-109	7.009484671502298E-4
110-114	0.0018383849907676359
115-119	0.08314327202658879
120-124	0.0010047783888087543
125-129	7.985163856546574E-4
130-134	0.0019427997807460583
135-139	0.0012897794139137928
140-144	7.360386834544538E-4
145-149	0.0014070320879059559
150	0.0019256826020610709
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3368337E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.722703098526935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.1466763285061	24.262796752402803
2	12.165798787644242	7.961956433690029
3	4.437128603820264	4.3558452573837565
4	2.3493918590305154	3.0751380906060723
5	1.3374906120108316	2.1883154096948765
6	0.9337598238440721	1.8333087288589427
7	0.6747522950817746	1.5455803311907383
8	0.5144876487452938	1.3468341262201173
9	0.3942311546031186	1.1610278121840587
>10	2.5505704910462823	16.03679843446611
>50	0.26358525507519764	5.996396774258157
>100	0.19362731198222707	12.59346786032521
>500	0.021657664922820292	4.903113051030676
>1k	0.015532592964234617	9.542654606575002
>5k	0.0011595157444225124	2.47588581463395
>10k+	1.5005497868997034E-4	0.7208805164795677
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT	28027	0.11993579175103475	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.2792946712468246E-5	0.0	0.0	4.279294671246824E-6	4.279294671246824E-6
2	6.418942006870236E-5	0.0	0.0	8.558589342493648E-6	8.558589342493648E-6
3	8.98651880961833E-5	0.0	0.0	1.2837884013740473E-5	1.2837884013740473E-5
4	1.3265813480865156E-4	0.0	0.0	2.1396473356234123E-5	2.1396473356234123E-5
5	1.4121672415114521E-4	0.0	0.0	2.1396473356234123E-5	2.1396473356234123E-5
6	1.6689249217862614E-4	0.0	0.0	2.1396473356234123E-5	2.1396473356234123E-5
7	1.7117178684987298E-4	0.0	0.0	2.1396473356234123E-5	2.1396473356234123E-5
8	1.8828896553486026E-4	0.0	0.0	2.1396473356234123E-5	2.5675768027480945E-5
9	2.3536120691857533E-4	0.0	0.0	2.1396473356234123E-5	2.5675768027480945E-5
10-11	2.974109796516543E-4	0.0	2.139647335623412E-6	2.1396473356234123E-5	2.781541536310436E-5
12-13	4.043933464328249E-4	0.0	4.279294671246824E-6	2.1396473356234123E-5	3.209471003435118E-5
14-15	4.856999451865146E-4	0.0	4.279294671246824E-6	2.995506269872777E-5	3.4234357369974594E-5
16-17	5.584479545977106E-4	0.0	4.279294671246824E-6	2.995506269872777E-5	3.851365204122142E-5
18-19	6.269166693376598E-4	2.139647335623412E-6	4.279294671246824E-6	3.209471003435118E-5	3.851365204122142E-5
20-21	6.932457367419856E-4	4.279294671246824E-6	4.279294671246824E-6	3.637400470559801E-5	3.851365204122142E-5
22-23	7.574351568106879E-4	4.279294671246824E-6	4.279294671246824E-6	4.493259404809165E-5	3.851365204122142E-5
24-25	8.280435188862604E-4	8.558589342493648E-6	4.279294671246824E-6	5.777047806183213E-5	4.065329937684483E-5
26-27	8.922329389549629E-4	1.7117178684987297E-5	4.279294671246824E-6	6.418942006870236E-5	4.707224138371507E-5
28-29	9.713998903730292E-4	1.7117178684987297E-5	4.279294671246824E-6	6.418942006870236E-5	5.777047806183213E-5
30-31	0.0010676840204760826	1.7117178684987297E-5	4.279294671246824E-6	6.418942006870236E-5	6.846871473994919E-5
32-33	0.0012367161599903323	1.7117178684987297E-5	4.279294671246824E-6	6.418942006870236E-5	7.274800941119601E-5
34-35	0.0014378430095389329	1.7117178684987297E-5	4.279294671246824E-6	6.418942006870236E-5	7.274800941119601E-5
36-37	0.0016860421004712487	1.7117178684987297E-5	4.279294671246824E-6	6.846871473994919E-5	7.274800941119601E-5
38-39	0.0019577773120954224	1.7117178684987297E-5	4.279294671246824E-6	6.846871473994919E-5	8.77255407605599E-5
40-41	0.002338634537836389	1.7117178684987297E-5	4.279294671246824E-6	7.488765674681943E-5	1.0056342477430036E-4
42-43	0.0029056410817765938	1.7117178684987297E-5	4.279294671246824E-6	8.130659875368967E-5	1.0912201411679401E-4
44-45	0.0035346973984498767	1.7117178684987297E-5	8.558589342493648E-6	9.414448276743013E-5	1.1982025079491109E-4
46-47	0.004302830791938682	2.1396473356234123E-5	8.558589342493648E-6	9.414448276743013E-5	1.1982025079491109E-4
48-49	0.0053255822183666725	2.1396473356234123E-5	8.558589342493648E-6	9.414448276743013E-5	1.219598981305345E-4
50-51	0.0065409019050007705	2.1396473356234123E-5	8.558589342493648E-6	9.414448276743013E-5	1.2837884013740472E-4
52-53	0.008143497759382706	2.1396473356234123E-5	8.558589342493648E-6	9.414448276743013E-5	1.2837884013740472E-4
54-55	0.009891589632587034	2.1396473356234123E-5	1.2837884013740473E-5	9.414448276743013E-5	1.2837884013740472E-4
56-57	0.01203979555755294	2.1396473356234123E-5	1.4977531349363884E-5	9.414448276743013E-5	1.2837884013740472E-4
58-59	0.014656584249020374	2.1396473356234123E-5	1.7117178684987297E-5	9.628413010305355E-5	1.2837884013740472E-4
60-61	0.018007271976606634	2.1396473356234123E-5	1.7117178684987297E-5	1.048427194455472E-4	1.3051848747302813E-4
62-63	0.02221809793311351	2.1396473356234123E-5	1.7117178684987297E-5	1.0698236678117061E-4	1.3479778214427497E-4
64-65	0.027605729924213264	2.995506269872777E-5	1.7117178684987297E-5	1.0912201411679401E-4	1.390770768155218E-4
66-67	0.03384280190755551	2.995506269872777E-5	1.7117178684987297E-5	1.1126166145241743E-4	1.4977531349363884E-4
68-69	0.041676050803272816	2.995506269872777E-5	1.925682602061071E-5	1.1340130878804085E-4	1.5191496082926225E-4
70-71	0.05156764043585986	2.995506269872777E-5	2.1396473356234123E-5	1.1554095612366426E-4	1.583339028361325E-4
72-73	0.06395405886178379	2.995506269872777E-5	2.1396473356234123E-5	1.240995454661579E-4	1.6261319750737933E-4
74-75	0.07988373327550008	2.995506269872777E-5	2.1396473356234123E-5	1.2837884013740472E-4	1.6261319750737933E-4
76-77	0.09975035878676347	2.995506269872777E-5	2.3536120691857536E-5	1.3051848747302813E-4	1.6903213951424955E-4
78-79	0.12393051332664365	2.995506269872777E-5	2.5675768027480945E-5	1.3265813480865156E-4	1.7117178684987298E-4
80-81	0.15343838973222612	2.995506269872777E-5	2.9955062698727768E-5	1.3479778214427497E-4	1.7117178684987298E-4
82-83	0.19156904490037097	3.209471003435118E-5	3.4234357369974594E-5	1.3693742947989838E-4	1.7117178684987298E-4
84-85	0.2392061531806906	3.4234357369974594E-5	3.4234357369974594E-5	1.3693742947989838E-4	1.754510815211198E-4
86-87	0.29851503767683596	3.4234357369974594E-5	3.4234357369974594E-5	1.390770768155218E-4	1.925682602061071E-4
88-89	0.3712223937886552	3.4234357369974594E-5	3.4234357369974594E-5	1.583339028361325E-4	1.925682602061071E-4
90-91	0.46005627186906795	4.0653299376844826E-5	3.4234357369974594E-5	1.6689249217862614E-4	1.947079075417305E-4
92-93	0.5688744560642034	4.7072241383715065E-5	3.4234357369974594E-5	1.6903213951424955E-4	1.9898720221297734E-4
94-95	0.7004649068523789	4.921188871933848E-5	3.4234357369974594E-5	1.7117178684987298E-4	2.1396473356234121E-4
96-97	0.8594899157779178	5.3491183390585304E-5	3.4234357369974594E-5	1.754510815211198E-4	2.1824402823358803E-4
98-99	1.049563347190688	5.563083072620872E-5	3.4234357369974594E-5	1.797303761923666E-4	2.268026175760817E-4
100-101	1.277420810903232	5.563083072620872E-5	3.4234357369974594E-5	1.8187002352799004E-4	2.3108191224732852E-4
102-103	1.5434281866099413	5.563083072620872E-5	3.4234357369974594E-5	1.9042861287048367E-4	2.3536120691857533E-4
104-105	1.8572652388571766	5.563083072620872E-5	3.4234357369974594E-5	1.968475548773539E-4	2.3536120691857533E-4
106-107	2.220549541030669	5.563083072620872E-5	3.4234357369974594E-5	2.0112684954860075E-4	2.3536120691857533E-4
108-109	2.6393362950902324	5.563083072620872E-5	3.851365204122142E-5	2.0112684954860075E-4	2.3536120691857533E-4
110-111	3.109436071552717	5.991012539745554E-5	3.851365204122142E-5	2.0540614421984756E-4	2.3750085425419876E-4
112-113	3.637511732221253	5.991012539745554E-5	3.851365204122142E-5	2.096854388910944E-4	2.3964050158982217E-4
114-115	4.232832229353762	5.991012539745554E-5	4.065329937684483E-5	2.118250862267178E-4	2.481990909323158E-4
116-117	4.897654890889326	5.991012539745554E-5	4.7072241383715065E-5	2.1610438089796462E-4	2.503387382679392E-4
118-119	5.634318351365781	5.991012539745554E-5	4.7072241383715065E-5	2.1824402823358803E-4	2.5247838560356263E-4
120-121	6.43095184736509	5.991012539745554E-5	4.7072241383715065E-5	2.2038367556921146E-4	2.5247838560356263E-4
122-123	7.2887321849218445	5.991012539745554E-5	4.7072241383715065E-5	2.2466297024045827E-4	2.5247838560356263E-4
124-125	8.210599239475192	5.991012539745554E-5	4.7072241383715065E-5	2.2680261757608168E-4	2.631766222816797E-4
126-127	9.188270436188933	5.991012539745554E-5	4.7072241383715065E-5	2.3108191224732852E-4	2.6745591695292656E-4
128-129	10.22900987776751	5.991012539745554E-5	4.7072241383715065E-5	2.3322155958295192E-4	2.6959556428854994E-4
130-131	11.324505034312025	5.991012539745554E-5	4.7072241383715065E-5	2.3536120691857533E-4	2.7387485895979675E-4
132-133	12.45640842991951	5.991012539745554E-5	4.7072241383715065E-5	2.3536120691857533E-4	2.80293800966667E-4
134-135	13.642211681558683	6.204977273307896E-5	4.7072241383715065E-5	2.3536120691857533E-4	2.8243344830229043E-4
136-137	14.85167087414051	6.418942006870236E-5	4.7072241383715065E-5	2.3536120691857533E-4	3.059695689941479E-4
138-139	16.09096530917027	6.632906740432578E-5	4.921188871933848E-5	2.3964050158982214E-4	3.102488636653948E-4
>>END_MODULE
