##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261773_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29894103
Sequences flagged as poor quality	0
Sequence length	150
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.947799838650454	32.0	32.0	32.0	32.0	32.0
2	31.58002285601277	32.0	32.0	32.0	32.0	32.0
3	35.34190208015273	37.0	32.0	37.0	32.0	37.0
4	36.1521439863909	37.0	37.0	37.0	32.0	37.0
5	36.389469990118116	37.0	37.0	37.0	37.0	37.0
6	39.76337038779856	41.0	41.0	41.0	37.0	41.0
7	39.87586334334902	41.0	41.0	41.0	37.0	41.0
8	40.05722168683235	41.0	41.0	41.0	37.0	41.0
9	40.14168801117732	41.0	41.0	41.0	37.0	41.0
10-14	40.14779826643402	41.0	41.0	41.0	37.0	41.0
15-19	40.118829068060684	41.0	41.0	41.0	37.0	41.0
20-24	40.10064724805424	41.0	41.0	41.0	37.0	41.0
25-29	40.04115712051972	41.0	41.0	41.0	37.0	41.0
30-34	39.988353836875454	41.0	41.0	41.0	37.0	41.0
35-39	39.97596434989202	41.0	41.0	41.0	37.0	41.0
40-44	39.91323161628232	41.0	41.0	41.0	37.0	41.0
45-49	39.869411254788275	41.0	41.0	41.0	37.0	41.0
50-54	39.87772276023803	41.0	41.0	41.0	37.0	41.0
55-59	39.813328608655695	41.0	41.0	41.0	37.0	41.0
60-64	39.75404195937908	41.0	41.0	41.0	37.0	41.0
65-69	39.72917821953045	41.0	41.0	41.0	37.0	41.0
70-74	39.69174142472179	41.0	41.0	41.0	37.0	41.0
75-79	39.370824553591724	41.0	40.2	41.0	36.0	41.0
80-84	39.69513466251187	41.0	41.0	41.0	37.0	41.0
85-89	39.683335867277904	41.0	41.0	41.0	37.0	41.0
90-94	39.648594741243784	41.0	41.0	41.0	37.0	41.0
95-99	39.62301232453772	41.0	41.0	41.0	37.0	41.0
100-104	39.55179672057729	41.0	41.0	41.0	37.0	41.0
105-109	39.45098707929119	41.0	41.0	41.0	37.0	41.0
110-114	39.37328794913164	41.0	41.0	41.0	37.0	41.0
115-119	39.20543143241328	41.0	41.0	41.0	37.0	41.0
120-124	39.06959571926276	41.0	41.0	41.0	37.0	41.0
125-129	38.99958601199708	41.0	41.0	41.0	37.0	41.0
130-134	38.78127626040494	41.0	41.0	41.0	32.0	41.0
135-139	38.59563905295971	41.0	41.0	41.0	32.0	41.0
140-144	38.32949644951715	41.0	38.6	41.0	32.0	41.0
145-149	38.07439081212773	41.0	37.0	41.0	32.0	41.0
150	35.11605700294804	37.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	19.0
16	89.0
17	217.0
18	483.0
19	1056.0
20	2485.0
21	5122.0
22	9320.0
23	16921.0
24	28484.0
25	46377.0
26	71017.0
27	102386.0
28	140310.0
29	184883.0
30	235089.0
31	292043.0
32	355829.0
33	430253.0
34	521109.0
35	640897.0
36	809675.0
37	1079793.0
38	1594663.0
39	3188885.0
40	2.0136697E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.237331173957514	12.321802421323754	6.3508552363063675	32.09001116841236
2	26.74096393963412	12.81092589759864	38.008719909425096	22.439390253342143
3	23.434260596479515	17.834105943904724	25.650801430636673	33.080832028979096
4	25.414754876572143	26.439806539771403	24.986172021953628	23.15926656170282
5	27.2905839419428	29.719725315716122	25.033362768348084	17.956327973992998
6	22.313105497588246	30.47597038185272	26.878890847647778	20.332033272911257
7	18.494972068571517	22.58181488168419	42.38266991988353	16.54054312986076
8	19.52497119582414	22.099070843503817	33.66259559619501	24.713362364477035
9	19.430501059021573	20.55114013623356	36.5823018673616	23.43605693738327
10-14	24.26980580483994	25.977916471747932	27.618974401816214	22.133303321595914
15-19	24.279330844212158	25.385629966597072	27.668165792664333	22.666873396526437
20-24	24.244012658046362	25.292749652959856	27.57515115411534	22.88808653487844
25-29	23.97531300323981	25.281826001041726	28.000461418182915	22.74239957753555
30-34	24.017284741277518	25.315356396715277	27.547365235981182	23.119993626026023
35-39	24.17770483445446	25.151199991655854	27.593325516428933	23.077769657460745
40-44	24.07358529298231	25.386342946242145	27.630708539025694	22.90936322174985
45-49	23.960814205397487	25.45538244349599	27.594516473325587	22.989286877780938
50-54	23.81342353731509	25.522414003191248	27.308464991550217	23.35569746794345
55-59	24.2117977560038	25.37235477766601	27.11770024677201	23.298147219558178
60-64	23.81098191803893	25.396621692362764	27.460125948175417	23.332270441422885
65-69	24.075046561790845	25.331490185576726	27.59616786173306	22.997295390899367
70-74	23.881257302305333	25.30459463300985	27.46887324142959	23.34527482325523
75-79	24.00338243358795	25.405593207841708	27.000563866047223	23.590460492523118
80-84	23.717323127687873	25.259238360167778	27.486763087598643	23.53667542454571
85-89	24.165959443372838	24.937016260103313	27.36053562221009	23.536488674313755
90-94	24.42083096357806	25.348033305896365	27.0591504364035	23.171985294122074
95-99	24.69102469851517	25.422176706696405	26.60552819352041	23.28127040126801
100-104	24.121960217494607	25.274811660644115	27.033179981976563	23.57004813988472
105-109	24.5459210806183	25.32782363896951	26.57483747575557	23.551417804656623
110-114	24.662848593509633	25.612944746047294	26.54104082972728	23.183165830715787
115-119	24.491922315079663	26.098938831114722	25.95114938803689	23.45798946576873
120-124	24.791870969149933	26.06017396109534	25.685536921962587	23.46241814779214
125-129	24.583907590203445	25.961552723539594	25.885474204625925	23.569065481631036
130-134	24.5369228911148	26.147794302479628	25.59148223934201	23.72380056706356
135-139	24.407432593683957	26.333112959174688	25.174990857439596	24.08446358970176
140-144	24.477633339385857	26.520333200106457	24.911850149334974	24.090183311172712
145-149	24.145303188118973	26.56578125543175	25.116609991559148	24.172305564890124
150	24.346478140213513	25.935086856643828	25.10374094287468	24.61469406026798
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	296.0
1	216.0
2	107.5
3	80.5
4	83.5
5	86.5
6	94.5
7	108.5
8	122.5
9	145.0
10	162.5
11	196.0
12	250.5
13	286.0
14	378.0
15	513.0
16	669.5
17	1031.0
18	1661.0
19	2472.0
20	3910.0
21	6604.0
22	10254.5
23	14764.0
24	22057.0
25	32997.5
26	46669.5
27	64175.5
28	90758.0
29	127004.0
30	165213.5
31	211205.0
32	271038.5
33	350750.5
34	454259.5
35	561284.0
36	626873.5
37	779912.0
38	930921.5
39	847599.5
40	742522.5
41	786537.0
42	870097.0
43	973483.5
44	1090903.0
45	1164316.0
46	1218214.5
47	1268247.5
48	1329873.0
49	1391790.5
50	1403910.5
51	1355963.5
52	1306442.5
53	1250676.5
54	1154750.0
55	1111598.5
56	1045055.5
57	906844.0
58	792075.5
59	684548.0
60	568815.5
61	455850.0
62	360098.5
63	275830.0
64	211329.5
65	157001.5
66	109703.5
67	81256.5
68	59561.5
69	40925.0
70	28988.5
71	20816.0
72	16044.0
73	11482.0
74	7405.0
75	4898.5
76	3619.5
77	2338.0
78	1197.5
79	667.0
80	420.5
81	337.0
82	285.5
83	151.5
84	72.0
85	47.5
86	25.5
87	9.5
88	3.0
89	3.0
90	2.0
91	0.0
92	0.0
93	0.5
94	1.5
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.500486567534741
2	0.21477814537536047
3	0.0
4	0.0
5	3.3451413477768505E-6
6	0.007222160169850221
7	0.0
8	0.0
9	0.0
10-14	7.091699657286924E-5
15-19	6.021254425998331E-5
20-24	3.746558309510073E-5
25-29	5.994493295216117E-4
30-34	1.231012015981881E-4
35-39	2.903582689870307E-4
40-44	0.0013059431821720825
45-49	0.002678789191299702
50-54	6.59661873781595E-4
55-59	3.7800097229878416E-4
60-64	6.302246299211587E-4
65-69	5.726881987393969E-4
70-74	9.700909908552868E-4
75-79	4.1345947058521877E-4
80-84	0.09075502282172508
85-89	6.589928455120396E-4
90-94	0.0012350261855992133
95-99	4.275090642458815E-4
100-104	4.6363659080187147E-4
105-109	7.513187467106808E-4
110-114	0.0018739481830245916
115-119	0.08313278374668075
120-124	0.001055726609358374
125-129	8.389614500224342E-4
130-134	0.0019850068757707832
135-139	0.0013327043129542973
140-144	7.861082167275599E-4
145-149	0.001465840938595816
150	0.002174341876054953
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.9894103E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.80976731489165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.89236197683555	22.7660647885405
2	12.270355381493982	7.560935883497163
3	4.471866466175977	4.13331495858446
4	2.300359628235917	2.8349417954607707
5	1.440258479251186	2.218701430951438
6	0.963471548173943	1.781060052825456
7	0.6765564241787531	1.4591182203039763
8	0.5015828620861892	1.2362921016010302
9	0.4096939503137166	1.1360317751535949
>10	2.5668794481180988	15.209843518295274
>50	0.26527988906659433	5.671230615164862
>100	0.19895660421665068	12.193424495646418
>500	0.023007667251251668	4.900068391495254
>1k	0.017000529916118744	9.936102216920109
>5k	0.0016584012802508463	3.5145127800730216
>10k+	7.107434058181744E-4	3.4483569754865955
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT	59396	0.19868801549255385	No Hit
GTCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAA	32162	0.10758643602719908	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.348683752109906E-5	0.0	0.0	6.690282695553702E-6	3.345141347776851E-6
2	6.021254425998331E-5	3.345141347776851E-6	0.0	2.3415989434437955E-5	1.0035424043330553E-5
3	7.359310965109072E-5	3.345141347776851E-6	0.0	2.3415989434437955E-5	1.3380565391107404E-5
4	1.1707994717218978E-4	3.345141347776851E-6	0.0	2.6761130782214807E-5	3.0106272129991656E-5
5	1.3715079525885088E-4	3.345141347776851E-6	0.0	3.3451413477768505E-5	3.0106272129991656E-5
6	1.639119260410657E-4	3.345141347776851E-6	0.0	3.3451413477768505E-5	3.3451413477768505E-5
7	1.8732791547550364E-4	3.345141347776851E-6	0.0	3.3451413477768505E-5	3.3451413477768505E-5
8	2.0739876356216474E-4	3.345141347776851E-6	0.0	3.3451413477768505E-5	3.3451413477768505E-5
9	2.6761130782214804E-4	3.345141347776851E-6	0.0	3.3451413477768505E-5	3.3451413477768505E-5
10-11	3.211335693865777E-4	3.345141347776851E-6	0.0	5.1849690890541186E-5	4.014169617332221E-5
12-13	3.880363963421147E-4	6.690282695553702E-6	0.0	5.686740291220646E-5	5.1849690890541186E-5
14-15	4.532666526237633E-4	6.690282695553702E-6	1.6725706738884254E-6	6.021254425998331E-5	5.686740291220646E-5
16-17	5.184969089054119E-4	6.690282695553702E-6	3.345141347776851E-6	6.021254425998331E-5	6.188511493387174E-5
18-19	5.82054594513172E-4	6.690282695553702E-6	3.345141347776851E-6	6.021254425998331E-5	6.690282695553701E-5
20-21	6.556477041642628E-4	6.690282695553702E-6	3.345141347776851E-6	6.52302562816486E-5	6.690282695553701E-5
22-23	7.242231017936882E-4	8.362853369442126E-6	3.345141347776851E-6	6.857539762942544E-5	6.690282695553701E-5
24-25	8.028339234664442E-4	1.0035424043330553E-5	3.345141347776851E-6	7.19205389772023E-5	6.690282695553701E-5
26-27	8.479933316614317E-4	1.0035424043330553E-5	3.345141347776851E-6	7.359310965109072E-5	7.024796830331387E-5
28-29	8.931527398564191E-4	1.0035424043330553E-5	3.345141347776851E-6	7.8610821672756E-5	7.024796830331387E-5
30-31	9.83471556246394E-4	1.0035424043330553E-5	3.345141347776851E-6	8.697367504219812E-5	7.024796830331387E-5
32-33	0.0010905160793752535	1.0035424043330553E-5	3.345141347776851E-6	9.199138706386339E-5	7.359310965109072E-5
34-35	0.0012477377227207652	1.1707994717218979E-5	3.345141347776851E-6	9.366395773775182E-5	7.693825099886758E-5
36-37	0.001473534763695703	1.5053136064995828E-5	3.345141347776851E-6	9.700909908552867E-5	8.697367504219812E-5
38-39	0.0017913231917345038	1.839827741277268E-5	3.345141347776851E-6	1.0035424043330553E-4	1.0202681110719396E-4
40-41	0.0021960852948155023	2.0070848086661105E-5	3.345141347776851E-6	1.1038966447663608E-4	1.1707994717218977E-4
42-43	0.0026911662142864766	2.0070848086661105E-5	3.345141347776851E-6	1.1707994717218978E-4	1.2042508851996663E-4
44-45	0.0033167076463207473	2.0070848086661105E-5	6.690282695553702E-6	1.1875251784607821E-4	1.2209765919385504E-4
46-47	0.004139612417873853	2.0070848086661105E-5	6.690282695553702E-6	1.2209765919385504E-4	1.2544280054163192E-4
48-49	0.005164898240967457	2.0070848086661105E-5	6.690282695553702E-6	1.2377022986774348E-4	1.304605125632972E-4
50-51	0.006528043340186524	2.0070848086661105E-5	6.690282695553702E-6	1.2377022986774348E-4	1.3715079525885088E-4
52-53	0.00831769396124714	2.0070848086661105E-5	6.690282695553702E-6	1.2711537121552034E-4	1.5053136064995827E-4
54-55	0.0103465221886738	2.3415989434437955E-5	6.690282695553702E-6	1.321330832371856E-4	1.5053136064995827E-4
56-57	0.012656342289313715	2.6761130782214807E-5	6.690282695553702E-6	1.3715079525885088E-4	1.5053136064995827E-4
58-59	0.015750598036007302	2.6761130782214807E-5	8.362853369442126E-6	1.3715079525885088E-4	1.5220393132384672E-4
60-61	0.019940387574097808	2.6761130782214807E-5	1.0035424043330553E-5	1.3715079525885088E-4	1.57221643345512E-4
62-63	0.025008276715979737	3.0106272129991656E-5	1.0035424043330553E-5	1.3715079525885088E-4	1.57221643345512E-4
64-65	0.03140418697292907	3.0106272129991656E-5	1.0035424043330553E-5	1.3715079525885088E-4	1.6558449671495414E-4
66-67	0.03928199484694356	3.0106272129991656E-5	1.0035424043330553E-5	1.3715079525885088E-4	1.789650621060615E-4
68-69	0.0490732235718864	3.0106272129991656E-5	1.0035424043330553E-5	1.4049593660662773E-4	1.8565534480161522E-4
70-71	0.06149707853752963	3.0106272129991656E-5	1.3380565391107404E-5	1.4049593660662773E-4	1.906730568232805E-4
72-73	0.07698675554841033	3.0106272129991656E-5	1.6725706738884253E-5	1.45513648628293E-4	1.906730568232805E-4
74-75	0.09669131065748987	3.0106272129991656E-5	1.839827741277268E-5	1.5220393132384672E-4	1.9234562749716893E-4
76-77	0.12136340066801804	3.0106272129991656E-5	2.0070848086661105E-5	1.5554907267162355E-4	1.973633395188342E-4
78-79	0.15157838989181244	3.0106272129991656E-5	2.174341876054953E-5	1.57221643345512E-4	1.973633395188342E-4
80-81	0.18878974224448214	3.0106272129991656E-5	2.3415989434437955E-5	1.57221643345512E-4	1.973633395188342E-4
82-83	0.23688116683079602	3.3451413477768505E-5	2.3415989434437955E-5	1.57221643345512E-4	2.0070848086661106E-4
84-85	0.2968779494738477	3.3451413477768505E-5	2.3415989434437955E-5	1.6223935536717726E-4	2.0238105154049947E-4
86-87	0.37288290603668556	3.3451413477768505E-5	2.3415989434437955E-5	1.7561992075828468E-4	2.107439049099416E-4
88-89	0.4650298421732206	3.3451413477768505E-5	2.508856010832638E-5	1.8063763277994995E-4	2.1241647558383002E-4
90-91	0.5755750557225283	3.5123984151656933E-5	2.6761130782214807E-5	1.8063763277994995E-4	2.1408904625771846E-4
92-93	0.7119815570314989	3.679655482554536E-5	2.6761130782214807E-5	1.823102034538384E-4	2.1408904625771846E-4
94-95	0.8769438574557664	4.014169617332221E-5	2.843370145610323E-5	1.839827741277268E-4	2.3415989434437957E-4
96-97	1.0727935205147316	4.014169617332221E-5	3.0106272129991656E-5	1.8732791547550364E-4	2.3415989434437957E-4
98-99	1.306296094584273	4.348683752109906E-5	3.0106272129991656E-5	1.8900048614939205E-4	2.3583246501826798E-4
100-101	1.5831082136834813	4.348683752109906E-5	3.0106272129991656E-5	1.906730568232805E-4	2.3750503569215642E-4
102-103	1.904511066948555	4.515940819498749E-5	3.0106272129991656E-5	1.906730568232805E-4	2.3750503569215642E-4
104-105	2.280297555675111	4.683197886887591E-5	3.0106272129991656E-5	1.9569076884494576E-4	2.3750503569215642E-4
106-107	2.71408545023077	4.683197886887591E-5	3.0106272129991656E-5	2.057261928882763E-4	2.4085017703993325E-4
108-109	3.2082447832604313	4.683197886887591E-5	3.0106272129991656E-5	2.0739876356216474E-4	2.4085017703993325E-4
110-111	3.7602215393450678	5.3522261564429614E-5	3.0106272129991656E-5	2.0739876356216474E-4	2.4085017703993325E-4
112-113	4.373441143224802	5.686740291220646E-5	3.0106272129991656E-5	2.1241647558383002E-4	2.4085017703993325E-4
114-115	5.059509562805748	5.686740291220646E-5	3.0106272129991656E-5	2.157616169316069E-4	2.542307424310407E-4
116-117	5.814666859213002	5.686740291220646E-5	3.0106272129991656E-5	2.174341876054953E-4	2.542307424310407E-4
118-119	6.6458424927484865	5.686740291220646E-5	3.0106272129991656E-5	2.2077932895327217E-4	2.542307424310407E-4
120-121	7.545125204124707	5.686740291220646E-5	3.0106272129991656E-5	2.24124470301049E-4	2.542307424310407E-4
122-123	8.494294008420322	5.686740291220646E-5	3.0106272129991656E-5	2.2914218232271427E-4	2.542307424310407E-4
124-125	9.495329229313219	5.686740291220646E-5	3.0106272129991656E-5	2.308147529966027E-4	2.609210251265944E-4
126-127	10.556252515755364	5.686740291220646E-5	3.0106272129991656E-5	2.308147529966027E-4	2.6761130782214804E-4
128-129	11.67410509022465	5.686740291220646E-5	3.0106272129991656E-5	2.3415989434437957E-4	2.6761130782214804E-4
130-131	12.850469204578575	5.686740291220646E-5	3.0106272129991656E-5	2.3750503569215642E-4	2.7262901984381337E-4
132-133	14.055457693445426	5.686740291220646E-5	3.0106272129991656E-5	2.3750503569215642E-4	2.776467318654786E-4
134-135	15.296799505909242	6.355768560776017E-5	3.0106272129991656E-5	2.3750503569215642E-4	2.8433701456103235E-4
136-137	16.558105121936592	6.355768560776017E-5	3.0106272129991656E-5	2.425227477138217E-4	3.0106272129991655E-4
138-139	17.845196760043276	6.52302562816486E-5	3.177884280388008E-5	2.458678890615985E-4	3.1277071601713553E-4
>>END_MODULE
