##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261770_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20527915
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.716060544872676	32.0	32.0	32.0	32.0	32.0
2	31.41829869229291	32.0	32.0	32.0	32.0	32.0
3	35.020958046640395	37.0	32.0	37.0	32.0	37.0
4	35.941851376528014	37.0	37.0	37.0	32.0	37.0
5	36.3073892307134	37.0	37.0	37.0	37.0	37.0
6	39.615862059054706	41.0	41.0	41.0	37.0	41.0
7	39.71984914200979	41.0	41.0	41.0	37.0	41.0
8	39.99745132420901	41.0	41.0	41.0	37.0	41.0
9	40.01658044667469	41.0	41.0	41.0	37.0	41.0
10-14	40.0390843492873	41.0	41.0	41.0	37.0	41.0
15-19	39.99950268695091	41.0	41.0	41.0	37.0	41.0
20-24	39.95082393901183	41.0	41.0	41.0	37.0	41.0
25-29	39.86200961958387	41.0	41.0	41.0	37.0	41.0
30-34	39.82198669470329	41.0	41.0	41.0	37.0	41.0
35-39	39.73421680672392	41.0	41.0	41.0	37.0	41.0
40-44	39.61515634685743	41.0	41.0	41.0	37.0	41.0
45-49	39.58995247203625	41.0	41.0	41.0	37.0	41.0
50-54	39.62817985168002	41.0	41.0	41.0	37.0	41.0
55-59	39.577401436044525	41.0	41.0	41.0	37.0	41.0
60-64	39.52091964527328	41.0	41.0	41.0	37.0	41.0
65-69	39.49791052817589	41.0	41.0	41.0	37.0	41.0
70-74	39.4513125468417	41.0	41.0	41.0	37.0	41.0
75-79	39.09366830484245	41.0	40.2	41.0	35.0	41.0
80-84	39.503756947551665	41.0	41.0	41.0	37.0	41.0
85-89	39.49085328928924	41.0	41.0	41.0	37.0	41.0
90-94	39.447558965438034	41.0	41.0	41.0	37.0	41.0
95-99	39.4134105290284	41.0	41.0	41.0	37.0	41.0
100-104	39.32486587166792	41.0	41.0	41.0	37.0	41.0
105-109	39.20682927613447	41.0	41.0	41.0	37.0	41.0
110-114	39.11563900181777	41.0	41.0	41.0	37.0	41.0
115-119	38.92901601550864	41.0	41.0	41.0	35.0	41.0
120-124	38.76263133396645	41.0	41.0	41.0	32.0	41.0
125-129	38.67091847369789	41.0	41.0	41.0	32.0	41.0
130-134	38.4245680576912	41.0	37.0	41.0	32.0	41.0
135-139	38.20407285396495	41.0	37.0	41.0	32.0	41.0
140-144	37.89661813194375	41.0	37.0	41.0	32.0	41.0
145-149	37.59188368619025	41.0	37.0	41.0	30.0	41.0
150	34.45535739991129	37.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	8.0
16	42.0
17	135.0
18	318.0
19	841.0
20	2097.0
21	4348.0
22	7946.0
23	13842.0
24	23273.0
25	36818.0
26	55208.0
27	79525.0
28	108564.0
29	143997.0
30	183593.0
31	230849.0
32	284169.0
33	346682.0
34	423767.0
35	524100.0
36	666881.0
37	890171.0
38	1327047.0
39	2672746.0
40	1.2500946E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.967955841284905	12.179881034230275	5.1363398945201	30.715823229964716
2	27.011534905254692	12.850315308368856	38.28929912017491	21.848850666201542
3	23.423776842411907	17.62004080784629	26.062179232523125	32.89400311721867
4	25.476440252212658	26.561504176142588	24.779467374061127	23.18258819758363
5	27.459296280211603	29.718994841901868	24.767493435158904	18.05421544272762
6	22.764374189924457	30.668879726267846	26.08642868156422	20.480317402243475
7	18.529251509468935	22.412860731350456	42.5013499909757	16.556537768204905
8	19.62006857491372	21.876815058908807	33.46903959803029	25.03407676814718
9	19.673352115887074	20.02129295644492	36.32636339345715	23.978991534210852
10-14	24.537316823343012	25.900972969366542	27.22260631191043	22.339103895380017
15-19	24.506361739841136	25.23884364554585	27.40689328162564	22.847901332987373
20-24	24.42026539632597	25.163207064660607	27.39971331909165	23.016814219921773
25-29	24.203513203813586	25.19963825453869	27.68876760817896	22.908080933468764
30-34	24.257729892878622	25.243709397680362	27.24000310367974	23.258557605761276
35-39	24.3592037782721	25.06764977028925	27.30452299757036	23.26862345386829
40-44	24.273825714274246	25.297951131462533	27.368994625281623	23.059228528981595
45-49	24.164833888277908	25.336164177945342	27.313671757888976	23.18533017588778
50-54	23.949187508619417	25.42991799359571	27.106506902083506	23.51438759570138
55-59	24.33693343591272	25.26371769929434	26.953786323656736	23.445562541136205
60-64	23.96975489497552	25.21346510881602	27.299415421164085	23.517364575044372
65-69	24.234048170687146	25.24504340983874	27.30731192693417	23.21359649253994
70-74	24.099459090977202	25.168311267225498	27.205814625364223	23.52641501643308
75-79	24.154381430000456	25.270418167003356	26.83877728412182	23.736423118874367
80-84	23.90545911174699	25.10390396417136	27.255513250920476	23.735123673161173
85-89	24.325135037587803	24.851428085119483	27.15145795192988	23.671978925362836
90-94	24.52036993902878	25.222100295827797	26.85817077068026	23.399358994463157
95-99	24.796763833299888	25.305385236199985	26.448600365326264	23.449250565173863
100-104	24.249616783676593	25.166010718170757	26.84509603722774	23.739276460924913
105-109	24.63161492427873	25.24016472112482	26.410368853221676	23.71785150137477
110-114	24.695774767986975	25.463133591793085	26.413914167973168	23.427177472246765
115-119	24.594936908321717	25.905753999750846	25.88253521372867	23.616773878198767
120-124	24.85402565000412	25.885166182150996	25.60317572593074	23.657632441914142
125-129	24.70963298102962	25.85890683903006	25.675684720776527	23.755775459163793
130-134	24.688474096178997	26.033960226319408	25.368176100235534	23.90938957726606
135-139	24.565431395036306	26.257126755399344	24.917382526930933	24.26005932263342
140-144	24.685206997005697	26.432945349089753	24.584790854490652	24.297056799413898
145-149	24.351274218525067	26.545163380059577	24.613047791379987	24.49051461003537
150	24.516750323614918	26.00928589202651	24.545448433914256	24.92851535044431
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	170.0
1	130.0
2	81.5
3	81.0
4	97.5
5	106.5
6	116.5
7	129.5
8	137.5
9	156.0
10	165.5
11	185.0
12	227.0
13	276.5
14	322.5
15	388.5
16	579.5
17	887.0
18	1290.5
19	1859.0
20	2882.0
21	4788.5
22	7291.5
23	10144.5
24	14857.0
25	22409.5
26	31524.0
27	43399.5
28	62212.0
29	87061.5
30	113325.5
31	143335.5
32	183471.0
33	235296.0
34	294685.0
35	360072.0
36	401345.5
37	488663.0
38	575676.0
39	529475.0
40	477527.5
41	515304.0
42	577753.5
43	648142.0
44	725432.5
45	778997.5
46	820175.5
47	860145.5
48	905508.0
49	954291.5
50	975975.0
51	952558.5
52	921608.0
53	888431.0
54	831707.5
55	806454.5
56	758643.5
57	660190.0
58	580689.5
59	506496.5
60	420271.0
61	334303.0
62	264267.0
63	202862.5
64	154440.0
65	114351.5
66	79891.0
67	58903.0
68	42931.5
69	28829.5
70	19730.0
71	13674.5
72	10039.0
73	6863.5
74	4312.5
75	2684.5
76	1814.0
77	1118.0
78	534.5
79	294.0
80	200.0
81	148.0
82	98.5
83	50.5
84	25.5
85	13.5
86	6.0
87	3.0
88	0.5
89	1.0
90	1.0
91	0.0
92	0.5
93	0.5
94	1.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.4003906387960003
2	0.20970468749505247
3	0.0
4	0.0
5	0.0
6	0.006235411633378255
7	0.0
8	0.0
9	0.0
10-14	6.235411633378257E-5
15-19	4.189417191176016E-5
20-24	2.2408510557453106E-5
25-29	5.397528195143053E-4
30-34	1.1593968505812694E-4
35-39	2.689021266894373E-4
40-44	0.0012344166467953516
45-49	0.0025311874099244857
50-54	6.235411633378256E-4
55-59	3.604847350546804E-4
60-64	5.3877853644659E-4
65-69	5.027300629411219E-4
70-74	8.505491181155027E-4
75-79	3.809446794767028E-4
80-84	0.09127083778357423
85-89	5.455985179205974E-4
90-94	0.0011184769617372246
95-99	3.536647535806729E-4
100-104	4.082246053727327E-4
105-109	6.284125786764023E-4
110-114	0.0017566323710907804
115-119	0.08250911015561006
120-124	9.694116523767757E-4
125-129	7.4630082986996E-4
130-134	0.0017556580880230652
135-139	0.0011477054537686851
140-144	6.810238643330314E-4
145-149	0.0012217509669150521
150	0.002099580010926585
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.0527915E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.846100165799136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.8494246352086	26.43056648356712
2	11.553345955761488	8.051781008491904
3	4.136575947579018	4.324306194383217
4	2.1260685977072886	2.9634079726027314
5	1.299626607724684	2.2643459475456043
6	0.8479294668877162	1.7728221082021216
7	0.6179364840150917	1.5072873632664137
8	0.4664163938886301	1.3002233904331222
9	0.348377960104669	1.0925651964027663
>10	2.311365194306617	15.427255035496282
>50	0.23571542956784966	5.732074975096184
>100	0.1729475119540593	11.961832724653206
>500	0.019544853453470457	4.70770154820549
>1k	0.01341026126651539	8.783857820249171
>5k	0.001097991688648178	2.5277705339531615
>10k+	2.1670888591740346E-4	1.152201697451868
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAAT	32721	0.15939758129357023	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.358556872434439E-5	0.0	0.0	0.0	0.0
2	5.845698406292115E-5	0.0	0.0	0.0	0.0
3	6.819981474007467E-5	0.0	0.0	0.0	4.8714153385767625E-6
4	1.2178538346441906E-4	0.0	0.0	0.0	9.742830677153525E-6
5	1.4127104481872612E-4	0.0	0.0	0.0	9.742830677153525E-6
6	1.8511378286591696E-4	0.0	0.0	4.8714153385767625E-6	9.742830677153525E-6
7	2.0459944422022402E-4	0.0	0.0	4.8714153385767625E-6	9.742830677153525E-6
8	2.2408510557453108E-4	0.0	0.0	1.948566135430705E-5	1.4614246015730287E-5
9	3.117705816689128E-4	0.0	0.0	1.948566135430705E-5	1.948566135430705E-5
10-11	3.945846424247177E-4	0.0	0.0	1.948566135430705E-5	2.4357076692883812E-5
12-13	5.090629028812717E-4	4.8714153385767625E-6	0.0	1.948566135430705E-5	3.4099907370037335E-5
14-15	6.381554093535559E-4	9.742830677153525E-6	0.0	2.4357076692883812E-5	4.384273804719086E-5
16-17	7.063552240936305E-4	9.742830677153525E-6	0.0	3.166419970074895E-5	4.384273804719086E-5
18-19	8.159620692116077E-4	9.742830677153525E-6	0.0	3.4099907370037335E-5	4.384273804719086E-5
20-21	8.573690995895102E-4	9.742830677153525E-6	0.0	3.4099907370037335E-5	4.627844571647924E-5
22-23	9.158260836524313E-4	9.742830677153525E-6	0.0	3.4099907370037335E-5	4.8714153385767624E-5
24-25	9.694116523767757E-4	1.4614246015730287E-5	0.0	3.653561503932572E-5	4.8714153385767624E-5
26-27	0.0010473542977940038	1.4614246015730287E-5	0.0	3.89713227086141E-5	5.845698406292115E-5
28-29	0.0011106826971955018	1.4614246015730287E-5	0.0	4.140703037790248E-5	7.307123007865144E-5
30-31	0.001176446804266288	1.4614246015730287E-5	0.0	4.8714153385767624E-5	7.307123007865144E-5
32-33	0.001310410726077149	1.4614246015730287E-5	2.4357076692883813E-6	5.358556872434439E-5	7.79426454172282E-5
34-35	0.0014492460632265867	1.4614246015730287E-5	4.8714153385767625E-6	5.358556872434439E-5	7.79426454172282E-5
36-37	0.0016514097997775224	1.4614246015730287E-5	4.8714153385767625E-6	5.358556872434439E-5	8.281406075580497E-5
38-39	0.001936387597084263	1.4614246015730287E-5	4.8714153385767625E-6	5.358556872434439E-5	1.2178538346441906E-4
40-41	0.0022822580861232133	1.4614246015730287E-5	4.8714153385767625E-6	6.33283994014979E-5	1.3883533714943773E-4
42-43	0.0027377354202801404	1.4614246015730287E-5	4.8714153385767625E-6	7.307123007865144E-5	1.4857816782659123E-4
44-45	0.003271155399854296	1.4614246015730287E-5	4.8714153385767625E-6	7.307123007865144E-5	1.53449583165168E-4
46-47	0.0039507178395857545	1.4614246015730287E-5	4.8714153385767625E-6	7.79426454172282E-5	1.558852908344564E-4
48-49	0.004851929677222456	1.4614246015730287E-5	4.8714153385767625E-6	8.524976842509334E-5	1.6075670617303315E-4
50-51	0.006113626249913837	1.948566135430705E-5	4.8714153385767625E-6	8.768547609438172E-5	1.6075670617303315E-4
52-53	0.00774798609600634	1.948566135430705E-5	4.8714153385767625E-6	1.0229972211011201E-4	1.7780665985805182E-4
54-55	0.00966001661639772	2.4357076692883812E-5	4.8714153385767625E-6	1.0473542977940039E-4	1.802423675273402E-4
56-57	0.011898431964473741	2.6792784362172194E-5	4.8714153385767625E-6	1.0960684511797717E-4	1.85113782865917E-4
58-59	0.014809102629273357	3.4099907370037335E-5	4.8714153385767625E-6	1.1447826045655392E-4	1.9242090587378213E-4
60-61	0.01839933573380443	3.4099907370037335E-5	4.8714153385767625E-6	1.169139681258423E-4	1.948566135430705E-4
62-63	0.02264477420137408	3.4099907370037335E-5	4.8714153385767625E-6	1.169139681258423E-4	1.948566135430705E-4
64-65	0.02822741617938305	3.4099907370037335E-5	4.8714153385767625E-6	1.169139681258423E-4	1.9972802888164727E-4
66-67	0.03506688331474482	3.4099907370037335E-5	4.8714153385767625E-6	1.2422109113370745E-4	2.1190656722808917E-4
68-69	0.04370146700237214	3.4099907370037335E-5	4.8714153385767625E-6	1.3639962948014934E-4	2.1921369023595433E-4
70-71	0.05457203033040618	3.4099907370037335E-5	4.8714153385767625E-6	1.4614246015730287E-4	2.2652081324381947E-4
72-73	0.06850184249106643	3.4099907370037335E-5	4.8714153385767625E-6	1.558852908344564E-4	2.2895652091310784E-4
74-75	0.08569306722090383	3.4099907370037335E-5	7.307123007865144E-6	1.558852908344564E-4	2.2895652091310784E-4
76-77	0.10699333078883072	3.4099907370037335E-5	9.742830677153525E-6	1.558852908344564E-4	2.2895652091310784E-4
78-79	0.1334719088616647	3.4099907370037335E-5	9.742830677153525E-6	1.558852908344564E-4	2.2895652091310784E-4
80-81	0.16703596054445863	3.4099907370037335E-5	9.742830677153525E-6	1.558852908344564E-4	2.2895652091310784E-4
82-83	0.2096559733416667	3.4099907370037335E-5	9.742830677153525E-6	1.583209985037448E-4	2.2895652091310784E-4
84-85	0.2622770018289729	3.89713227086141E-5	9.742830677153525E-6	1.6562812151160993E-4	2.313922285823962E-4
86-87	0.330286831370843	3.89713227086141E-5	9.742830677153525E-6	1.7780665985805182E-4	2.338279362516846E-4
88-89	0.41402889674864696	3.89713227086141E-5	1.4614246015730287E-5	1.802423675273402E-4	2.3626364392097298E-4
90-91	0.5149086012875638	3.89713227086141E-5	1.7049953685018668E-5	1.802423675273402E-4	2.3869935159026137E-4
92-93	0.6378192816951941	3.89713227086141E-5	1.948566135430705E-5	1.802423675273402E-4	2.4357076692883812E-4
94-95	0.7875982534027446	3.89713227086141E-5	1.948566135430705E-5	1.802423675273402E-4	2.484421822674149E-4
96-97	0.9655924627513316	3.89713227086141E-5	1.948566135430705E-5	1.8267807519662858E-4	2.533135976059916E-4
98-99	1.1797130882508038	3.89713227086141E-5	1.948566135430705E-5	1.8998519820449374E-4	2.581850129445684E-4
100-101	1.4338670050026998	3.89713227086141E-5	1.948566135430705E-5	1.8998519820449374E-4	2.6549213595243354E-4
102-103	1.7335930122469816	3.89713227086141E-5	1.948566135430705E-5	1.9242090587378213E-4	2.727992589602987E-4
104-105	2.0854845706444127	3.89713227086141E-5	1.948566135430705E-5	1.948566135430705E-4	2.727992589602987E-4
106-107	2.494734608945916	4.8714153385767624E-5	1.948566135430705E-5	1.948566135430705E-4	2.727992589602987E-4
108-109	2.96097046387809	4.8714153385767624E-5	1.948566135430705E-5	1.9729232121235886E-4	2.727992589602987E-4
110-111	3.4857120170265707	4.8714153385767624E-5	1.948566135430705E-5	1.9972802888164725E-4	2.727992589602987E-4
112-113	4.075603878913177	4.8714153385767624E-5	2.4357076692883812E-5	2.0459944422022402E-4	2.727992589602987E-4
114-115	4.740895507410276	4.8714153385767624E-5	2.9228492031460574E-5	2.0703515188951241E-4	2.801063819681638E-4
116-117	5.483781475127893	4.8714153385767624E-5	2.9228492031460574E-5	2.0947085955880078E-4	2.8254208963745224E-4
118-119	6.2998044370312325	4.8714153385767624E-5	2.9228492031460574E-5	2.0947085955880078E-4	2.8741350497602896E-4
120-121	7.1853449315237325	4.8714153385767624E-5	2.9228492031460574E-5	2.1190656722808917E-4	2.8741350497602896E-4
122-123	8.132494215803213	4.8714153385767624E-5	3.166419970074895E-5	2.1434227489737755E-4	2.9228492031460574E-4
124-125	9.142847678393057	4.8714153385767624E-5	3.4099907370037335E-5	2.2408510557453108E-4	3.0446345866104766E-4
126-127	10.216103291542273	4.8714153385767624E-5	3.4099907370037335E-5	2.2895652091310784E-4	3.06899166330336E-4
128-129	11.351800706501367	4.8714153385767624E-5	3.4099907370037335E-5	2.2895652091310784E-4	3.06899166330336E-4
130-131	12.549165368231503	4.8714153385767624E-5	3.4099907370037335E-5	2.2895652091310784E-4	3.117705816689128E-4
132-133	13.777599916991083	5.1149861055056006E-5	3.4099907370037335E-5	2.2895652091310784E-4	3.142062893382012E-4
134-135	15.059210835586565	5.358556872434439E-5	3.4099907370037335E-5	2.3626364392097298E-4	3.239491200153547E-4
136-137	16.373182566276213	5.358556872434439E-5	3.4099907370037335E-5	2.3869935159026137E-4	3.5074190437752687E-4
138-139	17.71170866597996	6.089269173220953E-5	3.653561503932572E-5	2.4357076692883812E-4	3.604847350546804E-4
>>END_MODULE
