FastQCFastQC Report
Thu 27 Jun 2019
SRR8261769_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8261769_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26446792
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGTT474140.1792807233482231No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT452910.17125328470840623No Hit
CTTTAATGTAGAAAAGATTAATGGGGAATGGCATACTATTATCCTGGCCT436700.16512399689157006No Hit
GCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCC406930.15386743314652304No Hit
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC379660.14355616363602813No Hit
GGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATTTGAGT373950.141397111604311No Hit
CACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAA373620.14127233276535014No Hit
GAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAA368980.13951786666602134No Hit
AAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATG367330.13889397247121693No Hit
AGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGA302160.1142520423649114No Hit
GCTAGTTCTACGGGAAGGAACTTTAATGTAGAAAAGATTAATGGGGAATG282100.10666700142686492No Hit
GGCAACTTTAGACTTTTTCTGGAGCAAATCCATGTCTTGGAGAATTCCTT275600.10420923641702934No Hit
CATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAA266940.10093473718854067No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)