##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261784_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22674782
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.876201544076586	32.0	32.0	32.0	32.0	32.0
2	31.376546817517365	32.0	32.0	32.0	32.0	32.0
3	34.96035811942977	37.0	32.0	37.0	32.0	37.0
4	35.71518411952097	37.0	37.0	37.0	32.0	37.0
5	35.964160052343615	37.0	37.0	37.0	37.0	37.0
6	39.21226268018806	41.0	41.0	41.0	37.0	41.0
7	39.408176404959484	41.0	41.0	41.0	37.0	41.0
8	39.50362627521623	41.0	41.0	41.0	37.0	41.0
9	39.485764890705454	41.0	41.0	41.0	37.0	41.0
10-14	39.52782152437011	41.0	41.0	41.0	37.0	41.0
15-19	39.45203914198602	41.0	41.0	41.0	37.0	41.0
20-24	39.41793928603151	41.0	41.0	41.0	37.0	41.0
25-29	39.33718611274852	41.0	41.0	41.0	37.0	41.0
30-34	39.328847174804146	41.0	41.0	41.0	37.0	41.0
35-39	39.220165274356326	41.0	41.0	41.0	37.0	41.0
40-44	39.10223073368467	41.0	41.0	41.0	37.0	41.0
45-49	39.04114784433209	41.0	41.0	41.0	37.0	41.0
50-54	38.991193555907174	41.0	41.0	41.0	37.0	41.0
55-59	38.88003032620115	41.0	41.0	41.0	32.0	41.0
60-64	38.84399101168866	41.0	41.0	41.0	32.0	41.0
65-69	38.73588993270145	41.0	41.0	41.0	32.0	41.0
70-74	38.64520828469266	41.0	41.0	41.0	32.0	41.0
75-79	38.08781977264434	41.0	39.4	41.0	31.0	41.0
80-84	38.754428236619866	41.0	41.0	41.0	32.0	41.0
85-89	38.70518470254753	41.0	41.0	41.0	32.0	41.0
90-94	38.627338097451165	41.0	41.0	41.0	32.0	41.0
95-99	38.484535798403705	41.0	41.0	41.0	32.0	41.0
100-104	38.27834066938328	41.0	38.6	41.0	32.0	41.0
105-109	38.12307519428411	41.0	37.0	41.0	32.0	41.0
110-114	37.905981843618164	41.0	37.0	41.0	31.0	41.0
115-119	37.56168119278942	41.0	37.0	41.0	27.0	41.0
120-124	37.23686157600103	41.0	37.0	41.0	27.0	41.0
125-129	36.91094027717664	41.0	37.0	41.0	27.0	41.0
130-134	36.51578506024887	41.0	37.0	41.0	25.0	41.0
135-139	36.03097163183311	41.0	34.0	41.0	22.0	41.0
140-144	35.55008948707864	41.0	32.0	41.0	22.0	41.0
145-149	34.87776243229152	41.0	32.0	41.0	22.0	41.0
150	29.935410404386687	32.0	22.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	14.0
13	97.0
14	716.0
15	3476.0
16	12264.0
17	26256.0
18	38283.0
19	48163.0
20	59834.0
21	72309.0
22	86363.0
23	101289.0
24	118253.0
25	136821.0
26	158641.0
27	182162.0
28	210753.0
29	241663.0
30	280589.0
31	327854.0
32	384669.0
33	458356.0
34	554713.0
35	683225.0
36	873930.0
37	1191531.0
38	1847413.0
39	3855511.0
40	1.0719633E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.34005883688308	22.78352047671528	6.903223916463738	32.97319676993789
2	26.34648402882796	26.057177462461766	30.345242315975334	17.251096192734938
3	21.095545703592652	26.204917868670137	27.894243922609707	24.8052925051275
4	23.569986251687006	34.67263764652731	21.089697797315097	20.66767830447058
5	26.58938292137955	35.820975366743355	18.748652684100243	18.840989027776846
6	22.0263198120273	36.66921251988222	19.264908478502683	22.0395591895878
7	21.036173872669057	20.277994318603795	34.45929711641711	24.22653469231004
8	19.99073684589338	23.567212244863036	26.8988826441639	29.543168265079682
9	21.38576679590569	21.98761602206363	29.37706302975702	27.24955415227366
10-14	24.501561562579756	27.10693195339722	23.62349783208482	24.768008651938207
15-19	24.38406221151004	26.61641764384836	24.19846898065387	24.801051163987726
20-24	24.187043071602467	26.873203338832713	24.418879110999434	24.52087447856539
25-29	24.054283551272135	26.612442388468494	24.651544447264033	24.68172961299534
30-34	23.994263231416184	26.741180985379692	24.669799345143755	24.59475643806037
35-39	23.87029682907844	26.887478142306676	24.566348048083853	24.67587698053103
40-44	23.88560474229586	26.660789099626957	24.77213777078531	24.68146838729187
45-49	23.82050790980533	26.653247410413222	24.766115987509842	24.76012869227161
50-54	23.909458437237706	26.75043475844087	24.702097820999803	24.638008983321615
55-59	24.00290464332335	26.620162007269915	24.64831684138403	24.728616508022704
60-64	23.871914451031245	26.58500187826273	24.941542936934326	24.601540733771703
65-69	24.10678754365129	26.585472479687734	24.964273971465026	24.343466005195953
70-74	24.16069143939822	26.645632483639236	24.832572252746818	24.36110382421572
75-79	24.073676518287236	26.72705547399169	24.79097045462907	24.408297553092005
80-84	23.93511501778944	26.897023572208173	24.74847103680277	24.41939037319962
85-89	24.066736345401655	26.778134119671925	24.55565914211891	24.59947039280751
90-94	23.93791221444714	26.969977121362497	24.661864205904543	24.430246458285815
95-99	23.916658654800287	27.10811962231033	24.546805029393994	24.428416693495393
100-104	24.25180956741418	27.179118695162686	24.293381840080766	24.275689897342364
105-109	24.371453107552256	27.455495873400178	24.096422014049278	24.07662900499829
110-114	24.483183450319224	27.4480868538378	24.025566506670337	24.04316318917264
115-119	24.79687135594407	27.5116668665805	23.880480085693133	23.810981691782292
120-124	25.100470401915477	27.600114763841855	23.70974736219219	23.589667472050483
125-129	25.63233536824608	27.79573760022151	23.400768320783705	23.171158710748703
130-134	26.30331913450523	27.709604403733323	23.157821416040463	22.82925504572098
135-139	26.708272813334588	27.763309801332582	23.134488679631918	22.39392870570091
140-144	27.067472086743336	27.851744079637626	22.85808109563474	22.2227027379843
145-149	27.714586161921694	27.49779953356021	22.876976130881864	21.91063817363623
150	27.562329099373517	27.4585158099935	23.07941996689565	21.899735123737337
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	130.0
1	106.5
2	85.5
3	89.0
4	114.5
5	152.0
6	163.5
7	184.0
8	221.0
9	264.5
10	318.0
11	356.5
12	410.0
13	477.0
14	563.0
15	715.5
16	910.5
17	1172.5
18	1609.0
19	2279.0
20	3427.5
21	5253.0
22	7927.5
23	11323.0
24	16105.5
25	22527.0
26	32090.5
27	45948.5
28	62468.5
29	86655.5
30	111011.0
31	136211.5
32	173801.0
33	223808.5
34	273681.5
35	309193.0
36	352056.0
37	411860.0
38	448251.0
39	463641.0
40	484331.0
41	539392.5
42	614575.0
43	693555.5
44	756202.0
45	819655.0
46	902193.5
47	959695.5
48	1011907.5
49	1071020.5
50	1156522.5
51	1173420.0
52	1125827.5
53	1084116.5
54	1003222.0
55	939083.5
56	874726.0
57	775221.5
58	681847.0
59	581534.0
60	483586.5
61	397015.5
62	315615.5
63	254036.0
64	196427.0
65	143988.0
66	106426.5
67	80619.5
68	64404.0
69	50139.0
70	36443.0
71	26593.5
72	20762.5
73	14689.0
74	9613.5
75	6508.5
76	4226.0
77	2655.0
78	1718.0
79	1184.0
80	765.5
81	456.5
82	306.0
83	232.5
84	163.0
85	111.0
86	84.5
87	70.5
88	59.0
89	38.5
90	31.5
91	35.0
92	31.5
93	23.0
94	18.0
95	17.5
96	16.0
97	15.5
98	17.0
99	18.0
100	42.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.5436091072452207
2	0.026880082022398274
3	0.0
4	0.0
5	3.0871300107758475E-4
6	0.0
7	3.263537439963039E-4
8	0.0
9	0.0
10-14	8.220586200123115E-4
15-19	1.4377205478756093E-4
20-24	9.825893805726555E-4
25-29	2.9989262961822524E-4
30-34	0.002442360857096664
35-39	1.8787391208435875E-4
40-44	4.1367542144396367E-4
45-49	4.5248505586514565E-4
50-54	2.9107225815886563E-5
55-59	0.0020560285871767145
60-64	4.6483357590824904E-4
65-69	6.059595192580021E-4
70-74	5.971391477986426E-4
75-79	5.142276560806627E-4
80-84	1.6494094629002388E-4
85-89	0.0010628547608528276
90-94	0.001179283664116374
95-99	3.6163522983374217E-4
100-104	2.355039179649004E-4
105-109	4.824743188269682E-4
110-114	2.355039179649004E-4
115-119	5.909648877770908E-5
120-124	1.9581224639778235E-4
125-129	1.5082835195504856E-4
130-134	4.1367542144396367E-4
135-139	8.09710099969208E-4
140-144	3.175333725369443E-4
145-149	1.940481721059104E-4
150	0.0018566881921951884
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.2674782E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.812450672946333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.90867348972986	23.194035790219818
2	12.42988658576194	7.9085030775973815
3	4.839704283902364	4.618885614098731
4	2.4086045518696335	3.0649445398794692
5	1.515020126671712	2.4098251524132372
6	0.9679273258810619	1.8475284185752883
7	0.7070617401122102	1.5745356711033098
8	0.5331526266868473	1.3568713310101679
9	0.4212692775509613	1.2061447300905925
>10	2.7257925392284816	16.761431445993914
>50	0.2885119408235559	6.421866935417757
>100	0.21440608197078936	13.674229073861039
>500	0.023751123730262724	5.1918509097072825
>1k	0.015573472741006662	9.209711163688548
>5k	6.07021744539651E-4	1.3053904239504477
>10k+	5.7811594718061364E-5	0.254245722392925
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3230557189039349E-5	0.0	0.0	0.0	0.0
2	1.3230557189039349E-5	0.0	0.0	8.820371459359566E-6	0.0
3	3.528148583743826E-5	0.0	0.0	8.820371459359566E-6	0.0
4	6.615278594519674E-5	0.0	0.0	8.820371459359566E-6	0.0
5	1.01434271782635E-4	0.0	0.0	8.820371459359566E-6	0.0
6	1.4553612907943284E-4	0.0	0.0	8.820371459359566E-6	0.0
7	1.6758705772783173E-4	0.0	0.0	8.820371459359566E-6	0.0
8	1.8522780064655087E-4	0.0	0.0	8.820371459359566E-6	0.0
9	2.9989262961822524E-4	0.0	0.0	1.3230557189039349E-5	0.0
10-11	3.947116228063405E-4	0.0	0.0	1.764074291871913E-5	2.2050928648398914E-6
12-13	5.159917303725345E-4	0.0	0.0	1.764074291871913E-5	4.410185729679783E-6
14-15	6.372718379387286E-4	0.0	0.0	1.764074291871913E-5	4.410185729679783E-6
16-17	7.232704596674843E-4	0.0	0.0	1.764074291871913E-5	4.410185729679783E-6
18-19	8.31320010044639E-4	2.2050928648398914E-6	0.0	1.764074291871913E-5	6.615278594519674E-6
20-21	9.195237246382346E-4	4.410185729679783E-6	0.0	1.764074291871913E-5	8.820371459359566E-6
22-23	0.001071675132312187	4.410185729679783E-6	0.0	2.6461114378078697E-5	8.820371459359566E-6
24-25	0.0012172112613916199	4.410185729679783E-6	0.0	2.6461114378078697E-5	8.820371459359566E-6
26-27	0.0013914135977139714	4.410185729679783E-6	0.0	2.6461114378078697E-5	1.764074291871913E-5
28-29	0.0016008974198737612	6.615278594519674E-6	0.0	2.6461114378078697E-5	1.764074291871913E-5
30-31	0.001803765963439031	8.820371459359566E-6	0.0	2.8666207242918586E-5	1.764074291871913E-5
32-33	0.002068377107219818	8.820371459359566E-6	0.0	3.307639297259837E-5	1.764074291871913E-5
34-35	0.0024344225227832398	8.820371459359566E-6	0.0	3.528148583743826E-5	1.764074291871913E-5
36-37	0.00284015960991378	8.820371459359566E-6	0.0	4.4101857296797825E-5	1.764074291871913E-5
38-39	0.003411278661907312	8.820371459359566E-6	0.0	5.2922228756157394E-5	1.764074291871913E-5
40-41	0.004108088007196718	8.820371459359566E-6	0.0	5.512732162099728E-5	1.764074291871913E-5
42-43	0.004928382552917157	8.820371459359566E-6	0.0	5.733241448583717E-5	1.764074291871913E-5
44-45	0.005832470627501513	8.820371459359566E-6	0.0	5.9537507350677064E-5	1.764074291871913E-5
46-47	0.006824762416679463	1.1025464324199458E-5	0.0	6.174260021551696E-5	1.764074291871913E-5
48-49	0.008125767206935	1.3230557189039349E-5	0.0	6.394769308035685E-5	1.764074291871913E-5
50-51	0.009724459533943922	1.3230557189039349E-5	0.0	6.615278594519674E-5	1.764074291871913E-5
52-53	0.01177299080538018	1.3230557189039349E-5	0.0	6.615278594519674E-5	1.764074291871913E-5
54-55	0.01401116006319267	1.543565005387924E-5	0.0	6.835787881003663E-5	1.764074291871913E-5
56-57	0.016805012722944813	1.764074291871913E-5	0.0	7.71782502693962E-5	1.764074291871913E-5
58-59	0.020033268677070414	1.764074291871913E-5	0.0	7.938334313423608E-5	1.764074291871913E-5
60-61	0.02409725482697033	1.9845835783559024E-5	0.0	7.938334313423608E-5	1.764074291871913E-5
62-63	0.029347580938154115	2.2050928648398913E-5	0.0	8.158843599907597E-5	1.764074291871913E-5
64-65	0.03571147894608204	2.2050928648398913E-5	0.0	8.379352886391587E-5	1.764074291871913E-5
66-67	0.04344694471594038	2.2050928648398913E-5	0.0	8.599862172875576E-5	1.764074291871913E-5
68-69	0.053447040857989285	2.2050928648398913E-5	0.0	8.820371459359565E-5	1.764074291871913E-5
70-71	0.06542510529979957	2.2050928648398913E-5	0.0	9.702408605295522E-5	1.764074291871913E-5
72-73	0.08043737752362956	2.6461114378078697E-5	0.0	9.702408605295522E-5	1.764074291871913E-5
74-75	0.09956214794038593	2.6461114378078697E-5	0.0	9.702408605295522E-5	1.764074291871913E-5
76-77	0.12360868563146495	2.6461114378078697E-5	0.0	1.01434271782635E-4	1.764074291871913E-5
78-79	0.15304006009848298	2.6461114378078697E-5	0.0	1.0584445751231479E-4	2.2050928648398913E-5
80-81	0.18883312748056408	2.6461114378078697E-5	0.0	1.0804955037715467E-4	2.2050928648398913E-5
82-83	0.2345645484044786	2.6461114378078697E-5	0.0	1.1025464324199456E-4	2.2050928648398913E-5
84-85	0.2917845031542089	2.6461114378078697E-5	0.0	1.1907501470135413E-4	2.2050928648398913E-5
86-87	0.3643761602647382	2.6461114378078697E-5	0.0	1.1907501470135413E-4	2.2050928648398913E-5
88-89	0.4529591508310863	2.6461114378078697E-5	0.0	1.256902932958738E-4	2.2050928648398913E-5
90-91	0.5591983199662074	2.6461114378078697E-5	0.0	1.3230557189039348E-4	2.2050928648398913E-5
92-93	0.6885600928820397	2.6461114378078697E-5	0.0	1.3230557189039348E-4	2.2050928648398913E-5
94-95	0.8462242327180918	2.6461114378078697E-5	0.0	1.3671575762007327E-4	2.6461114378078697E-5
96-97	1.0368567159763653	2.6461114378078697E-5	0.0	1.3671575762007327E-4	2.6461114378078697E-5
98-99	1.264989008494106	2.8666207242918586E-5	0.0	1.3671575762007327E-4	2.8666207242918586E-5
100-101	1.5345880723351608	3.087130010775848E-5	0.0	1.3671575762007327E-4	3.087130010775848E-5
102-103	1.8488226259463048	3.087130010775848E-5	0.0	1.4333103621459294E-4	3.087130010775848E-5
104-105	2.221763366898081	3.528148583743826E-5	0.0	1.4553612907943284E-4	3.087130010775848E-5
106-107	2.653871159599241	3.528148583743826E-5	0.0	1.4774122194427273E-4	3.087130010775848E-5
108-109	3.1479508821738618	3.528148583743826E-5	0.0	1.5876668626847216E-4	3.087130010775848E-5
110-111	3.6973762305630986	3.528148583743826E-5	0.0	1.5876668626847216E-4	3.087130010775848E-5
112-113	4.310248715952374	3.528148583743826E-5	0.0	1.5876668626847216E-4	3.087130010775848E-5
114-115	5.0015585596368695	3.969167156711804E-5	0.0	1.5876668626847216E-4	3.969167156711804E-5
116-117	5.763960597283802	4.4101857296797825E-5	0.0	1.5876668626847216E-4	3.969167156711804E-5
118-119	6.593812015480458	4.4101857296797825E-5	0.0	1.6097177913331205E-4	3.969167156711804E-5
120-121	7.482111625152559	4.4101857296797825E-5	0.0	1.6317687199815195E-4	4.4101857296797825E-5
122-123	8.436354536947698	4.851204302647761E-5	0.0	1.6317687199815195E-4	4.4101857296797825E-5
124-125	9.459310347504111	4.851204302647761E-5	0.0	1.6538196486299184E-4	4.4101857296797825E-5
126-127	10.536491596699804	5.07171358913175E-5	0.0	1.7199724345751152E-4	4.4101857296797825E-5
128-129	11.679719787383181	5.2922228756157394E-5	0.0	1.786125220520312E-4	4.4101857296797825E-5
130-131	12.86643681954693	5.2922228756157394E-5	0.0	1.8302270778171098E-4	4.4101857296797825E-5
132-133	14.079954550389944	5.512732162099728E-5	0.0	1.9184307924107055E-4	4.4101857296797825E-5
134-135	15.339997535588214	5.733241448583717E-5	4.410185729679783E-6	1.9625326497075033E-4	4.4101857296797825E-5
136-137	16.626958089387585	6.174260021551696E-5	4.410185729679783E-6	2.0066345070043012E-4	4.4101857296797825E-5
138-139	17.933451355783706	6.835787881003663E-5	6.615278594519674E-6	2.1168891502462955E-4	4.851204302647761E-5
>>END_MODULE
