##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261784_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22674782
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.103890612928495	32.0	32.0	32.0	32.0	32.0
2	31.50097954635242	32.0	32.0	32.0	32.0	32.0
3	35.5620933422866	37.0	37.0	37.0	32.0	37.0
4	36.2160800046501	37.0	37.0	37.0	37.0	37.0
5	36.385117528362564	37.0	37.0	37.0	37.0	37.0
6	39.812039074951194	41.0	41.0	41.0	37.0	41.0
7	39.901401742252695	41.0	41.0	41.0	37.0	41.0
8	40.04422274048765	41.0	41.0	41.0	37.0	41.0
9	40.11225228097011	41.0	41.0	41.0	37.0	41.0
10-14	40.155527554796336	41.0	41.0	41.0	37.0	41.0
15-19	40.12074504619273	41.0	41.0	41.0	37.0	41.0
20-24	40.098770149146304	41.0	41.0	41.0	37.0	41.0
25-29	40.0396867939017	41.0	41.0	41.0	37.0	41.0
30-34	39.987945674626545	41.0	41.0	41.0	37.0	41.0
35-39	39.9764967089871	41.0	41.0	41.0	37.0	41.0
40-44	39.91187868531658	41.0	41.0	41.0	37.0	41.0
45-49	39.86631841488047	41.0	41.0	41.0	37.0	41.0
50-54	39.87016053340667	41.0	41.0	41.0	37.0	41.0
55-59	39.80854724865712	41.0	41.0	41.0	37.0	41.0
60-64	39.74836618936403	41.0	41.0	41.0	37.0	41.0
65-69	39.726047491878866	41.0	41.0	41.0	37.0	41.0
70-74	39.684034457310325	41.0	41.0	41.0	37.0	41.0
75-79	39.364981528819115	41.0	40.2	41.0	36.0	41.0
80-84	39.68976249473975	41.0	41.0	41.0	37.0	41.0
85-89	39.67483319575024	41.0	41.0	41.0	37.0	41.0
90-94	39.6437909568436	41.0	41.0	41.0	37.0	41.0
95-99	39.6174753785946	41.0	41.0	41.0	37.0	41.0
100-104	39.54595392361435	41.0	41.0	41.0	37.0	41.0
105-109	39.440217991952466	41.0	41.0	41.0	37.0	41.0
110-114	39.371111342988875	41.0	41.0	41.0	37.0	41.0
115-119	39.199009242955455	41.0	41.0	41.0	37.0	41.0
120-124	39.06562762984888	41.0	41.0	41.0	37.0	41.0
125-129	38.990561567471744	41.0	41.0	41.0	37.0	41.0
130-134	38.77409000889182	41.0	41.0	41.0	32.0	41.0
135-139	38.58691179478594	41.0	41.0	41.0	32.0	41.0
140-144	38.32160190117814	41.0	38.6	41.0	32.0	41.0
145-149	38.06633411514166	41.0	37.0	41.0	32.0	41.0
150	35.13132937727913	41.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	12.0
16	54.0
17	127.0
18	279.0
19	748.0
20	1915.0
21	3908.0
22	7515.0
23	13117.0
24	22480.0
25	36270.0
26	54801.0
27	78634.0
28	107553.0
29	141252.0
30	178772.0
31	221931.0
32	270050.0
33	326610.0
34	394390.0
35	483087.0
36	611939.0
37	814645.0
38	1203425.0
39	2403862.0
40	1.5297403E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.33725864166647	10.82438190664549	4.426507639632742	34.411851812055296
2	25.705192434817288	12.126771662974255	40.45839215360259	21.70964374860587
3	23.817366799821933	15.603307674578746	25.621542910533822	34.9577826150655
4	26.40550193602743	25.032796346178763	24.09203757725212	24.469664140541685
5	28.083088075554596	28.38829056879136	24.91120752561149	18.617413830042555
6	22.82723368866152	29.67875534046389	26.286073326287106	21.207937644587485
7	18.17209532598814	22.164005810507902	43.17583295839405	16.488065905109913
8	19.410034460309255	20.8455763764344	34.71087836698937	25.033510796266974
9	19.76155713426484	19.157524866170707	36.7508318271814	24.330086172383048
10-14	24.666715161107874	25.375924753844625	27.059680330606135	22.897679754441366
15-19	24.968879941810908	24.426827130749334	27.25997845851967	23.34431446892009
20-24	24.651318517464254	24.62706778325418	27.30390993827353	23.417703761008035
25-29	24.44426258683567	24.797282546324688	27.310889973787717	23.447564893051922
30-34	24.46660288825496	24.9106984925069	26.827735998866615	23.79496262037152
35-39	24.700704935021452	24.79939724928835	26.933057678257814	23.566840137432386
40-44	24.590328617302802	24.63103076601726	27.20321427810031	23.57542633857962
45-49	24.53274011182848	24.523252641706055	27.28839944817783	23.655607798287633
50-54	24.300305226496018	24.98061865334382	27.04492336525608	23.67415275490408
55-59	24.694529765932486	24.755678102335832	26.888604509246584	23.661187622485098
60-64	24.21712687553209	24.58875275241512	27.19775121377935	23.99636915827344
65-69	24.51433910657684	25.048352238176268	26.84888996999839	23.5884186852485
70-74	24.525417396919835	24.84186107385104	26.835940217114896	23.796781312114227
75-79	24.44823739626991	24.776458888638654	26.815514049714693	23.959789665376743
80-84	24.363478925580665	24.765628565165184	26.978003800688725	23.892888708565422
85-89	24.738317489181544	24.652572332216845	26.781008066926454	23.828102111675157
90-94	24.54957247884719	24.84115843289622	26.77668503486413	23.832584053392463
95-99	24.729787284074902	24.931430599920485	26.65667302835554	23.68210908764907
100-104	24.59330786460645	24.916604474621884	26.638950202811877	23.851137457959783
105-109	24.77367422272861	25.012213216968494	26.29705175685536	23.917060803447534
110-114	24.975280230378967	25.11900820070711	26.199827154561856	23.70588441435207
115-119	24.816888421806237	25.579065496792353	25.87663441839566	23.72741166300575
120-124	25.03013908832696	25.552360900691568	25.623857826944697	23.79364218403678
125-129	24.88149221439816	25.789807798124226	25.41779659908413	23.91090338839348
130-134	24.891408596247118	25.7793095397846	25.23962737280554	24.089654491162744
135-139	24.83311460875986	26.119962901734407	24.841575220554528	24.205347268951204
140-144	24.791729530688126	26.207892866212163	24.756778531327694	24.24359907177202
145-149	24.604046824723696	26.29966025899602	24.691573538934275	24.40471937734601
150	24.40442509606687	26.005192660768948	24.766482419972206	24.823899823191976
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	95.0
1	77.5
2	52.5
3	50.5
4	57.5
5	61.5
6	84.0
7	98.0
8	96.5
9	116.5
10	139.0
11	152.5
12	191.0
13	224.5
14	265.5
15	355.5
16	507.0
17	793.5
18	1189.5
19	1744.5
20	2889.0
21	4821.5
22	7426.5
23	10583.0
24	15530.0
25	22891.0
26	32518.5
27	44834.0
28	61877.5
29	85538.0
30	110493.0
31	135418.0
32	172010.0
33	218163.5
34	269529.5
35	322873.5
36	365427.0
37	420202.5
38	466811.5
39	473943.5
40	489018.5
41	550996.5
42	619195.0
43	684864.0
44	763613.5
45	853283.5
46	924874.0
47	970932.5
48	1018502.5
49	1082322.5
50	1173010.0
51	1195441.5
52	1159352.0
53	1106519.5
54	1006639.0
55	926590.5
56	845909.5
57	747640.5
58	673600.5
59	593331.0
60	491086.5
61	389638.5
62	306422.0
63	234509.0
64	177606.5
65	131000.0
66	91534.0
67	67119.5
68	48659.5
69	32515.0
70	22160.5
71	15098.5
72	11064.5
73	7678.5
74	4928.5
75	3138.5
76	2044.0
77	1249.5
78	670.0
79	361.0
80	224.5
81	141.5
82	86.5
83	49.0
84	31.0
85	17.0
86	7.5
87	4.5
88	3.0
89	1.5
90	1.0
91	1.5
92	2.0
93	0.5
94	0.5
95	0.5
96	0.5
97	1.0
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.4948633243750702
2	0.21456876630611046
3	0.0
4	0.0
5	0.0
6	0.00731208793980908
7	0.0
8	0.0
9	0.0
10-14	8.026538028017205E-5
15-19	7.585519455049225E-5
20-24	2.0286854356527E-5
25-29	6.324206336360809E-4
30-34	1.093726060960586E-4
35-39	2.9989262961822524E-4
40-44	0.001338050350384846
45-49	0.0029363016588207992
50-54	6.394769308035685E-4
55-59	3.748657870227815E-4
60-64	6.615278594519675E-4
65-69	5.89200813485219E-4
70-74	9.94937900615759E-4
75-79	4.1367542144396367E-4
80-84	0.09119117440688074
85-89	6.553535994304157E-4
90-94	0.0013301120160714225
95-99	3.986807899630523E-4
100-104	5.477450676262289E-4
105-109	7.338549054187158E-4
110-114	0.0018531600436114447
115-119	0.08285415930349407
120-124	0.0010619727237068915
125-129	8.635143658713014E-4
130-134	0.002087781924430409
135-139	0.0013177634960283191
140-144	7.938334313423608E-4
145-149	0.0014694738851293036
150	0.0020816076644088574
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.2674782E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.61495128808564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.94705148399049	26.692355877879674
2	12.055957919285088	8.587447080530973
3	4.327329625718874	4.623529014824026
4	2.1884021835775536	3.117593486674193
5	1.300649116047266	2.3161277455457507
6	0.8793187539067681	1.879013675225382
7	0.6296636601245572	1.5697808408249672
8	0.4851135905105161	1.3821837516176285
9	0.3669758210671402	1.1762843392090288
>10	2.372650335537485	16.302774431575234
>50	0.24077683057366103	5.951377906978294
>100	0.17544703167434567	12.291799604725517
>500	0.01868937990276175	4.574596645565345
>1k	0.011277899414789284	7.5680748143413705
>5k	5.932029402667503E-4	1.4949206356008156
>10k+	1.0316572874204165E-4	0.4721401488815756
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG	23390	0.10315424421721012	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.528148583743826E-5	0.0	0.0	0.0	0.0
2	5.733241448583717E-5	0.0	0.0	4.410185729679783E-6	0.0
3	1.1025464324199456E-4	0.0	0.0	1.764074291871913E-5	2.2050928648398913E-5
4	1.5435650053879238E-4	0.0	0.0	1.764074291871913E-5	2.6461114378078697E-5
5	1.8963798637623065E-4	0.0	4.410185729679783E-6	2.2050928648398913E-5	3.087130010775848E-5
6	2.3815002940270826E-4	0.0	4.410185729679783E-6	2.2050928648398913E-5	3.087130010775848E-5
7	2.6902132951046675E-4	0.0	4.410185729679783E-6	2.2050928648398913E-5	3.087130010775848E-5
8	2.9107225815886567E-4	0.0	4.410185729679783E-6	2.2050928648398913E-5	3.087130010775848E-5
9	4.101472728602198E-4	0.0	4.410185729679783E-6	3.528148583743826E-5	3.528148583743826E-5
10-11	5.07171358913175E-4	0.0	4.410185729679783E-6	4.851204302647761E-5	3.528148583743826E-5
12-13	6.240412807496892E-4	0.0	4.410185729679783E-6	6.174260021551696E-5	3.7486578702278155E-5
14-15	7.519366669104029E-4	0.0	4.410185729679783E-6	6.174260021551696E-5	3.969167156711804E-5
16-17	8.269098243149592E-4	0.0	4.410185729679783E-6	6.394769308035685E-5	3.969167156711804E-5
18-19	9.349593746921139E-4	0.0	6.615278594519674E-6	7.056297167487653E-5	3.969167156711804E-5
20-21	0.0010099325320966704	4.410185729679783E-6	8.820371459359566E-6	7.497315740455631E-5	5.733241448583717E-5
22-23	0.0010937260609605861	6.615278594519674E-6	8.820371459359566E-6	9.702408605295522E-5	6.174260021551696E-5
24-25	0.0011731094040948221	1.3230557189039349E-5	8.820371459359566E-6	1.1466482897167434E-4	6.394769308035685E-5
26-27	0.0012260316328509796	1.3230557189039349E-5	8.820371459359566E-6	1.256902932958738E-4	6.615278594519674E-5
28-29	0.001294389511661016	1.3230557189039349E-5	8.820371459359566E-6	1.278953861607137E-4	6.835787881003663E-5
30-31	0.0013671575762007327	1.3230557189039349E-5	8.820371459359566E-6	1.278953861607137E-4	7.056297167487653E-5
32-33	0.001519308983874685	1.3230557189039349E-5	8.820371459359566E-6	1.278953861607137E-4	7.497315740455631E-5
34-35	0.0017177673417102753	1.764074291871913E-5	8.820371459359566E-6	1.278953861607137E-4	7.497315740455631E-5
36-37	0.0019779682997613825	1.764074291871913E-5	8.820371459359566E-6	1.301004790255536E-4	7.93833431342361E-5
38-39	0.0023616544582435233	1.9845835783559024E-5	8.820371459359566E-6	1.3892085048491316E-4	1.2128010756619402E-4
40-41	0.0028511850742379795	2.2050928648398913E-5	8.820371459359566E-6	1.4994631480911262E-4	1.301004790255536E-4
42-43	0.0034796365407173484	2.4256021513238805E-5	8.820371459359566E-6	1.5435650053879238E-4	1.3451066475523337E-4
44-45	0.004138959307304476	3.087130010775848E-5	1.764074291871913E-5	1.5435650053879238E-4	1.3671575762007327E-4
46-47	0.004888690881350039	3.087130010775848E-5	1.764074291871913E-5	1.5656159340363227E-4	1.477412219442727E-4
48-49	0.005929494713554468	3.087130010775848E-5	1.764074291871913E-5	1.5876668626847216E-4	1.6097177913331205E-4
50-51	0.0072834217325661605	3.087130010775848E-5	1.764074291871913E-5	1.5876668626847216E-4	1.7199724345751152E-4
52-53	0.009073957138816154	3.087130010775848E-5	1.9845835783559024E-5	1.5876668626847216E-4	1.764074291871913E-4
54-55	0.011056335624307214	3.087130010775848E-5	2.2050928648398913E-5	1.6097177913331205E-4	1.764074291871913E-4
56-57	0.01361203825465665	4.189676443195793E-5	2.2050928648398913E-5	1.6758705772783173E-4	1.764074291871913E-4
58-59	0.016588913622190504	4.4101857296797825E-5	2.2050928648398913E-5	1.8081761491687108E-4	1.8081761491687108E-4
60-61	0.020394903906904155	4.4101857296797825E-5	2.2050928648398913E-5	1.8743289351139076E-4	1.8081761491687108E-4
62-63	0.02537841378144231	4.4101857296797825E-5	2.2050928648398913E-5	1.9184307924107055E-4	1.8522780064655087E-4
64-65	0.031512982131426885	4.630695016163772E-5	2.2050928648398913E-5	1.9404817210591044E-4	1.8963798637623065E-4
66-67	0.039043374264855116	4.851204302647761E-5	2.2050928648398913E-5	1.9625326497075033E-4	2.0286854356527E-4
68-69	0.048849422234798115	4.851204302647761E-5	2.2050928648398913E-5	2.0066345070043012E-4	2.0948382215978968E-4
70-71	0.06054523478990889	4.851204302647761E-5	2.2050928648398913E-5	2.072787292949498E-4	2.1168891502462958E-4
72-73	0.07537448430595717	4.851204302647761E-5	2.2050928648398913E-5	2.1830419361914925E-4	2.1609910075430933E-4
74-75	0.09439120517233639	4.851204302647761E-5	2.2050928648398913E-5	2.293296579433487E-4	2.2050928648398912E-4
76-77	0.11836938498460536	4.851204302647761E-5	2.2050928648398913E-5	2.3153475080818858E-4	2.293296579433487E-4
78-79	0.14774342703713755	4.851204302647761E-5	2.4256021513238805E-5	2.3373984367302847E-4	2.3373984367302847E-4
80-81	0.18316383372506073	5.512732162099728E-5	2.6461114378078697E-5	2.3373984367302847E-4	2.3373984367302847E-4
82-83	0.22884012732735423	6.174260021551696E-5	2.6461114378078697E-5	2.3373984367302847E-4	2.3373984367302847E-4
84-85	0.2860931584700572	7.056297167487653E-5	2.6461114378078697E-5	2.4035512226754815E-4	2.3815002940270826E-4
86-87	0.3585944067731279	7.056297167487653E-5	2.6461114378078697E-5	2.469704008620678E-4	2.3815002940270826E-4
88-89	0.44751036636206687	7.056297167487653E-5	2.6461114378078697E-5	2.469704008620678E-4	2.469704008620678E-4
90-91	0.5542986036205332	7.276806453971642E-5	2.6461114378078697E-5	2.469704008620678E-4	2.513805865917476E-4
92-93	0.6843836469960328	7.497315740455631E-5	2.6461114378078697E-5	2.469704008620678E-4	2.557907723214274E-4
94-95	0.8429871563925069	7.497315740455631E-5	2.6461114378078697E-5	2.469704008620678E-4	2.6902132951046675E-4
96-97	1.0347133657117409	7.497315740455631E-5	2.6461114378078697E-5	2.5358567945658753E-4	2.6902132951046675E-4
98-99	1.2637210800968228	7.938334313423608E-5	2.6461114378078697E-5	2.557907723214274E-4	2.6902132951046675E-4
100-101	1.5342330523839216	7.938334313423608E-5	2.6461114378078697E-5	2.602009580511072E-4	2.6902132951046675E-4
102-103	1.8489417009610059	7.938334313423608E-5	2.6461114378078697E-5	2.6461114378078696E-4	2.778417009698263E-4
104-105	2.222160283613752	7.938334313423608E-5	2.6461114378078697E-5	2.6681623664562683E-4	2.778417009698263E-4
106-107	2.6572140803823388	7.938334313423608E-5	2.8666207242918586E-5	2.7343151524014653E-4	2.8004679383466624E-4
108-109	3.1523169660462447	8.158843599907597E-5	3.087130010775848E-5	2.822518866995061E-4	2.866620724291859E-4
110-111	3.703321160926707	8.820371459359565E-5	3.087130010775848E-5	2.9327735102370554E-4	2.9107225815886567E-4
112-113	4.319327083276919	9.261390032327543E-5	3.087130010775848E-5	2.9989262961822524E-4	3.065079082127449E-4
114-115	5.0151838284487145	9.261390032327543E-5	3.307639297259837E-5	2.9989262961822524E-4	3.0871300107758475E-4
116-117	5.781872566624896	9.702408605295522E-5	3.528148583743826E-5	2.9989262961822524E-4	3.0871300107758475E-4
118-119	6.6170250280686265	9.702408605295522E-5	3.528148583743826E-5	2.9989262961822524E-4	3.1312318680726454E-4
120-121	7.51357168505523	9.702408605295522E-5	3.528148583743826E-5	3.065079082127449E-4	3.1312318680726454E-4
122-123	8.477644900841824	9.702408605295522E-5	3.528148583743826E-5	3.153282796721044E-4	3.175333725369443E-4
124-125	9.510953622398663	9.702408605295522E-5	3.528148583743826E-5	3.175333725369443E-4	3.285588368611438E-4
126-127	10.600487801823189	9.922917891779511E-5	3.528148583743826E-5	3.219435582666241E-4	3.3517411545566346E-4
128-129	11.756531992237015	1.01434271782635E-4	3.528148583743826E-5	3.219435582666241E-4	3.3517411545566346E-4
130-131	12.958069453545352	1.01434271782635E-4	3.528148583743826E-5	3.263537439963039E-4	3.3517411545566346E-4
132-133	14.189948992673887	1.0584445751231479E-4	3.528148583743826E-5	3.285588368611438E-4	3.417893940501831E-4
134-135	15.472620640851144	1.0584445751231479E-4	3.528148583743826E-5	3.307639297259837E-4	3.572250441040624E-4
136-137	16.78431351622256	1.0584445751231479E-4	3.528148583743826E-5	3.307639297259837E-4	3.792759727524613E-4
138-139	18.120954812266774	1.0804955037715467E-4	4.189676443195793E-5	3.3296902259082354E-4	3.814810656173012E-4
>>END_MODULE
