##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261783_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19686618
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.792315775111803	32.0	32.0	32.0	32.0	32.0
2	31.23568715560997	32.0	32.0	32.0	32.0	32.0
3	34.93232768574064	37.0	32.0	37.0	32.0	37.0
4	35.49302759874754	37.0	37.0	37.0	32.0	37.0
5	35.847190258885504	37.0	37.0	37.0	32.0	37.0
6	39.027247900071	41.0	41.0	41.0	37.0	41.0
7	39.22344945180528	41.0	41.0	41.0	37.0	41.0
8	39.40189660814265	41.0	41.0	41.0	37.0	41.0
9	39.373186496532824	41.0	41.0	41.0	37.0	41.0
10-14	39.38647622461106	41.0	41.0	41.0	37.0	41.0
15-19	39.30629605349177	41.0	41.0	41.0	37.0	41.0
20-24	39.28014885035103	41.0	41.0	41.0	37.0	41.0
25-29	39.12932536203018	41.0	41.0	41.0	37.0	41.0
30-34	39.128229429757816	41.0	41.0	41.0	37.0	41.0
35-39	39.07336048274011	41.0	41.0	41.0	37.0	41.0
40-44	38.96567567877835	41.0	41.0	41.0	37.0	41.0
45-49	38.88775834427224	41.0	41.0	41.0	32.0	41.0
50-54	38.81437877242297	41.0	41.0	41.0	32.0	41.0
55-59	38.68473632190151	41.0	41.0	41.0	32.0	41.0
60-64	38.61836376364899	41.0	41.0	41.0	32.0	41.0
65-69	38.4873256137748	41.0	41.0	41.0	32.0	41.0
70-74	38.37629078798603	41.0	41.0	41.0	32.0	41.0
75-79	37.8903901320176	41.0	38.6	41.0	31.0	41.0
80-84	38.55345018631438	41.0	41.0	41.0	32.0	41.0
85-89	38.478569198630254	41.0	41.0	41.0	32.0	41.0
90-94	38.37427648568179	41.0	41.0	41.0	32.0	41.0
95-99	38.20542408045912	41.0	38.6	41.0	32.0	41.0
100-104	37.966085449517024	41.0	37.0	41.0	32.0	41.0
105-109	37.777070881346916	41.0	37.0	41.0	29.0	41.0
110-114	37.5265153415381	41.0	37.0	41.0	27.0	41.0
115-119	37.145838071323375	41.0	37.0	41.0	27.0	41.0
120-124	36.775111144026866	41.0	37.0	41.0	27.0	41.0
125-129	36.39533851878469	41.0	37.0	41.0	24.0	41.0
130-134	35.941962992323006	41.0	33.0	41.0	22.0	41.0
135-139	35.40639031041289	41.0	32.0	41.0	22.0	41.0
140-144	34.85949426153339	41.0	32.0	41.0	22.0	41.0
145-149	34.11945458585116	37.8	30.0	41.0	12.0	41.0
150	29.24937487993113	32.0	22.0	37.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	16.0
13	155.0
14	1019.0
15	4228.0
16	11352.0
17	21021.0
18	29218.0
19	38236.0
20	49492.0
21	62469.0
22	76995.0
23	93226.0
24	110890.0
25	131163.0
26	153551.0
27	179392.0
28	208586.0
29	242717.0
30	283216.0
31	331326.0
32	391075.0
33	464511.0
34	561028.0
35	693328.0
36	886378.0
37	1202071.0
38	1830584.0
39	3561227.0
40	8068146.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.79850808314064	23.409823083187288	7.0180203272924695	30.7736485063796
2	27.356986346511437	25.84432290645501	29.417962847866537	17.380727899167017
3	21.326583367442797	26.404804522544197	27.867767841078646	24.40084426893436
4	23.662134349333137	34.950320060052974	20.937166556490304	20.450379034123586
5	26.394946679561432	36.22733419751088	18.5818270462038	18.795892076723888
6	22.24437940534022	36.64292160288781	19.028382630271995	22.08431636149998
7	21.076945263094053	20.347782570163503	34.38644642360702	24.18882574313543
8	20.150352894539832	23.7020548679311	26.639263280264796	29.50832895726427
9	21.376815459110347	22.184308142719082	29.0519123193227	27.386964078847875
10-14	24.596732712630942	27.1438199214937	23.51094647148451	24.748500894390844
15-19	24.486829369904502	26.614732635469473	24.173755349732883	24.724682644893146
20-24	24.326602776307787	26.888238875562738	24.333252025227118	24.45190632290236
25-29	24.132190800019682	26.665670454437052	24.56923599309706	24.63290275244621
30-34	24.104058015359588	26.81243620461091	24.504338825555173	24.579166954474328
35-39	23.916166771147314	26.966779867975443	24.488643035232265	24.628410325644975
40-44	23.978282582039608	26.71155832204373	24.683069739347584	24.627089356569083
45-49	23.927513577098207	26.69830609488305	24.705372204276735	24.668808123742007
50-54	24.000443103133655	26.807990518145598	24.597411199200074	24.594155179520673
55-59	24.079106354732183	26.678460490294633	24.59474579915133	24.64768735582186
60-64	23.96783348259273	26.58480928647832	24.898523522638786	24.548833708290164
65-69	24.213269835091733	26.554398036372973	24.92367818764652	24.308653940888778
70-74	24.236709854410364	26.63936716222222	24.814667009154977	24.309255974212434
75-79	24.13992105203192	26.70323948005113	24.745672115659882	24.411167352257067
80-84	23.99198239849187	26.912404486104442	24.698488856305392	24.397124259098295
85-89	24.111738934413644	26.79012896537853	24.509324463223674	24.58880763698415
90-94	23.999654096235897	26.976433944724604	24.632616940982263	24.39129501805724
95-99	24.002204713011928	27.020679272590996	24.603625382222827	24.37349063217425
100-104	24.35625389231018	27.159103269845836	24.299290195418163	24.185352642425826
105-109	24.38649576592743	27.393419667420527	24.10568041780119	24.11440414885085
110-114	24.47470879064116	27.39237318035518	24.064014709231753	24.06890331977191
115-119	24.773448766939854	27.414677956738366	23.9360804822503	23.87579279407148
120-124	25.082140973807853	27.531417363678646	23.759395346123522	23.62704631638998
125-129	25.600361253041065	27.593626861283628	23.532092299627777	23.27391958604753
130-134	26.21647054826771	27.491487687894338	23.272442264013115	23.019599499824835
135-139	26.533128882758835	27.397023451639324	23.38652957557637	22.68331809002548
140-144	26.863514461368275	27.366379349843157	23.221308888473988	22.548797300314586
145-149	27.445032418419984	26.85946515783824	23.370413201566045	22.325089222175734
150	27.214792597422804	26.708597394154577	23.65405366854752	22.422556339875097
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	78.0
1	63.0
2	53.0
3	65.5
4	79.5
5	96.5
6	112.5
7	128.5
8	142.5
9	175.0
10	229.0
11	268.0
12	292.0
13	340.0
14	440.0
15	538.0
16	662.5
17	887.0
18	1215.5
19	1732.5
20	2678.5
21	4205.5
22	6559.5
23	9579.0
24	13958.0
25	19898.5
26	27893.0
27	38466.5
28	51655.0
29	71425.5
30	91421.0
31	111629.0
32	140002.0
33	184438.5
34	238683.0
35	269688.5
36	299989.5
37	363745.0
38	400191.5
39	403976.5
40	419950.0
41	465702.5
42	527099.0
43	594798.5
44	651879.5
45	712427.0
46	785533.5
47	830507.5
48	879795.0
49	947106.5
50	1020420.0
51	1019437.0
52	965691.5
53	931279.5
54	874473.0
55	824150.0
56	769570.5
57	683627.5
58	597605.5
59	507722.0
60	423136.5
61	347288.0
62	276303.0
63	219689.0
64	168050.0
65	123840.0
66	91844.0
67	69432.0
68	54943.5
69	41802.0
70	30038.5
71	22230.5
72	17233.0
73	12131.5
74	7788.5
75	5088.5
76	3175.0
77	2022.5
78	1321.5
79	864.0
80	544.5
81	338.5
82	244.0
83	174.5
84	118.0
85	82.0
86	63.5
87	51.5
88	46.0
89	47.5
90	32.5
91	23.5
92	24.0
93	20.0
94	20.0
95	18.0
96	16.0
97	14.5
98	14.0
99	15.0
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.4517729759372586
2	0.0215831891490961
3	0.0
4	0.0
5	3.911286336738997E-4
6	0.0
7	3.3017352193251274E-4
8	0.0
9	0.0
10-14	7.436523632449209E-4
15-19	1.4019675700519002E-4
20-24	9.346450467012669E-4
25-29	2.5296371372675596E-4
30-34	0.0022258774970896475
35-39	1.9099268345634584E-4
40-44	4.002719004351077E-4
45-49	4.2871761924775497E-4
50-54	2.4382044696554788E-5
55-59	0.0019383725533761054
60-64	4.3278129336384746E-4
65-69	5.567233539046676E-4
70-74	5.42500494498344E-4
75-79	4.1754251542850074E-4
80-84	1.6356288317272169E-4
85-89	9.508997431656367E-4
90-94	0.0011053193595771504
95-99	3.291576034034896E-4
100-104	2.265498319721549E-4
105-109	4.429404786540786E-4
110-114	2.1639064668192373E-4
115-119	5.790735615431762E-5
120-124	1.8388125375318402E-4
125-129	1.493400237663981E-4
130-134	3.799535298546454E-4
135-139	8.371168679150476E-4
140-144	2.9258453635865744E-4
145-149	1.645788017017448E-4
150	0.001721981906694182
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	1.9686618E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.778982226191886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.45297702612956	25.149457876527038
2	11.871967238281904	8.020459406637137
3	4.462023063109472	4.521677932248994
4	2.2785086494177897	3.0786281268363207
5	1.4115205316837771	2.383986347582562
6	0.9043114597651987	1.832803243581017
7	0.6651642187208638	1.5727999225169615
8	0.496723623825182	1.342305476841636
9	0.3792267094975985	1.1528903051834953
>10	2.5628493071562555	16.590420346927868
>50	0.26835960532847397	6.294868729130017
>100	0.21147896130780153	14.209239263099892
>500	0.02169615793141409	5.053665892027509
>1k	0.012727342738178975	7.721055583051363
>5k	4.5056826958083545E-4	1.0084808041202054
>10k+	1.5536836882097772E-5	0.06726074368803044
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5238777935346741E-5	0.0	0.0	0.0	0.0
2	2.0318370580462324E-5	0.0	0.0	0.0	0.0
3	2.5397963225577903E-5	5.079592645115581E-6	0.0	0.0	5.079592645115581E-6
4	9.651226025719603E-5	5.079592645115581E-6	0.0	0.0	1.0159185290231162E-5
5	1.320694087730051E-4	5.079592645115581E-6	0.0	0.0	1.0159185290231162E-5
6	2.285816690302011E-4	5.079592645115581E-6	0.0	0.0	1.0159185290231162E-5
7	2.4382044696554786E-4	5.079592645115581E-6	0.0	0.0	1.0159185290231162E-5
8	2.793775954813569E-4	5.079592645115581E-6	0.0	0.0	1.0159185290231162E-5
9	3.2001433664228157E-4	5.079592645115581E-6	0.0	0.0	1.0159185290231162E-5
10-11	4.0636741160924645E-4	5.079592645115581E-6	0.0	0.0	1.2698981612788952E-5
12-13	5.43516413027367E-4	7.6193889676733714E-6	0.0	0.0	1.5238777935346741E-5
14-15	6.501878585747944E-4	1.0159185290231162E-5	0.0	0.0	1.5238777935346741E-5
16-17	7.543195077996637E-4	1.0159185290231162E-5	0.0	2.5397963225577905E-6	1.5238777935346741E-5
18-19	8.482919717343019E-4	1.0159185290231162E-5	0.0	5.079592645115581E-6	1.5238777935346741E-5
20-21	9.092470834756889E-4	1.0159185290231162E-5	0.0	5.079592645115581E-6	1.5238777935346741E-5
22-23	9.93060362120096E-4	1.0159185290231162E-5	0.0	5.079592645115581E-6	1.5238777935346741E-5
24-25	0.0010870328260547343	1.0159185290231162E-5	0.0	5.079592645115581E-6	1.5238777935346741E-5
26-27	0.001221642031150297	1.0159185290231162E-5	0.0	5.079592645115581E-6	2.0318370580462324E-5
28-29	0.0013816491994714378	1.0159185290231162E-5	0.0	5.079592645115581E-6	2.5397963225577903E-5
30-31	0.0015543553494053676	1.0159185290231162E-5	0.0	5.079592645115581E-6	2.5397963225577903E-5
32-33	0.0017981757963709156	1.0159185290231162E-5	0.0	1.0159185290231162E-5	2.793775954813569E-5
34-35	0.002059774817594368	1.0159185290231162E-5	0.0	1.0159185290231162E-5	3.0477555870693482E-5
36-37	0.00242550548804269	1.0159185290231162E-5	0.0	1.0159185290231162E-5	3.5557148515809065E-5
38-39	0.002819173918039147	1.5238777935346741E-5	0.0	1.0159185290231162E-5	3.5557148515809065E-5
40-41	0.003319513793583032	1.5238777935346741E-5	0.0	1.0159185290231162E-5	3.5557148515809065E-5
42-43	0.003982400633770615	1.5238777935346741E-5	0.0	1.5238777935346741E-5	3.5557148515809065E-5
44-45	0.004731640548925163	1.5238777935346741E-5	0.0	1.5238777935346741E-5	3.5557148515809065E-5
46-47	0.0056002508912399275	1.5238777935346741E-5	0.0	2.2858166903020115E-5	3.5557148515809065E-5
48-49	0.006801574551809762	1.5238777935346741E-5	0.0	2.5397963225577903E-5	3.5557148515809065E-5
50-51	0.008259417640957934	1.5238777935346741E-5	0.0	2.5397963225577903E-5	3.5557148515809065E-5
52-53	0.010098230178489775	1.5238777935346741E-5	0.0	3.5557148515809065E-5	3.5557148515809065E-5
54-55	0.012206261126212741	1.7778574257904533E-5	0.0	3.8096944838366856E-5	3.8096944838366856E-5
56-57	0.014593669669417064	2.2858166903020115E-5	0.0	4.063674116092465E-5	4.063674116092465E-5
58-59	0.01767698240500222	2.5397963225577903E-5	0.0	4.063674116092465E-5	4.063674116092465E-5
60-61	0.02146635851825844	3.0477555870693482E-5	0.0	4.317653748348244E-5	4.063674116092465E-5
62-63	0.02648753584795519	3.0477555870693482E-5	0.0	4.5716333806040224E-5	4.063674116092465E-5
64-65	0.0327811511352534	3.0477555870693482E-5	0.0	5.33357227737136E-5	4.063674116092465E-5
66-67	0.03975797163331965	3.0477555870693482E-5	0.0	6.0955111741386965E-5	4.063674116092465E-5
68-69	0.048438995463822174	3.0477555870693482E-5	0.0	6.349490806394476E-5	4.063674116092465E-5
70-71	0.05976648706242992	3.0477555870693482E-5	0.0	6.603470438650255E-5	4.063674116092465E-5
72-73	0.07349408618585478	3.0477555870693482E-5	0.0	6.603470438650255E-5	4.063674116092465E-5
74-75	0.09142250842679023	3.0477555870693482E-5	0.0	6.603470438650255E-5	5.0795926451155806E-5
76-77	0.113760017083686	3.0477555870693482E-5	0.0	6.603470438650255E-5	5.0795926451155806E-5
78-79	0.14070725606602413	3.0477555870693482E-5	0.0	7.873368599929149E-5	5.0795926451155806E-5
80-81	0.17479386251107226	3.0477555870693482E-5	0.0	8.635307496696486E-5	5.0795926451155806E-5
82-83	0.2170967100595948	3.0477555870693482E-5	0.0	8.635307496696486E-5	5.0795926451155806E-5
84-85	0.27053656448253327	3.0477555870693482E-5	0.0	8.635307496696486E-5	5.0795926451155806E-5
86-87	0.33806466910669974	3.0477555870693482E-5	0.0	9.651226025719603E-5	5.0795926451155806E-5
88-89	0.42007977195473595	3.0477555870693482E-5	0.0	1.0159185290231161E-4	5.0795926451155806E-5
90-91	0.519365489796165	3.0477555870693482E-5	0.0	1.0159185290231161E-4	5.0795926451155806E-5
92-93	0.6412909520568744	3.0477555870693482E-5	0.0	1.041316492248694E-4	5.0795926451155806E-5
94-95	0.7890918592518024	3.0477555870693482E-5	0.0	1.1683063083765835E-4	5.0795926451155806E-5
96-97	0.9665194905493671	3.0477555870693482E-5	0.0	1.2191022348277393E-4	5.0795926451155806E-5
98-99	1.1800503265720907	3.0477555870693482E-5	0.0	1.2191022348277393E-4	5.33357227737136E-5
100-101	1.43393090677129	3.0477555870693482E-5	0.0	1.2191022348277393E-4	5.8415315418829173E-5
102-103	1.72992384979482	3.0477555870693482E-5	0.0	1.2191022348277393E-4	6.0955111741386965E-5
104-105	2.0810608505737247	3.0477555870693482E-5	0.0	1.2191022348277393E-4	6.349490806394476E-5
106-107	2.485414203699183	3.0477555870693482E-5	0.0	1.244500198053317E-4	6.857450070906034E-5
108-109	2.9476723731826358	3.0477555870693482E-5	0.0	1.269898161278895E-4	7.111429703161813E-5
110-111	3.4663114812305498	3.3017352193251274E-5	0.0	1.269898161278895E-4	8.12734823218493E-5
112-113	4.046766691973197	4.063674116092465E-5	0.0	1.269898161278895E-4	8.12734823218493E-5
114-115	4.702183991176138	4.063674116092465E-5	0.0	1.269898161278895E-4	8.12734823218493E-5
116-117	5.431585557255188	4.063674116092465E-5	0.0	1.269898161278895E-4	8.12734823218493E-5
118-119	6.228731110645821	4.063674116092465E-5	0.0	1.3460920509556287E-4	8.12734823218493E-5
120-121	7.086892730889582	4.063674116092465E-5	0.0	1.3714900141812068E-4	8.12734823218493E-5
122-123	8.01069538708985	4.063674116092465E-5	0.0	1.4222859406323626E-4	8.12734823218493E-5
124-125	9.001650258058545	4.063674116092465E-5	0.0	1.4222859406323626E-4	8.12734823218493E-5
126-127	10.050055321843498	4.5716333806040224E-5	0.0	1.4730818670835184E-4	8.12734823218493E-5
128-129	11.161129859887563	4.5716333806040224E-5	0.0	1.4984798303090965E-4	8.12734823218493E-5
130-131	12.316064140625882	4.5716333806040224E-5	0.0	1.5238777935346743E-4	8.12734823218493E-5
132-133	13.504917401251959	5.0795926451155806E-5	0.0	1.5238777935346743E-4	8.12734823218493E-5
134-135	14.744094186213193	5.0795926451155806E-5	0.0	1.57467371998583E-4	8.12734823218493E-5
136-137	16.015597498767946	5.0795926451155806E-5	0.0	1.625469646436986E-4	8.12734823218493E-5
138-139	17.30506987030479	6.0955111741386965E-5	0.0	1.7016635361137192E-4	8.635307496696488E-5
>>END_MODULE
