##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261782_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21498551
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.798950357165932	32.0	32.0	32.0	32.0	32.0
2	31.234865177657788	32.0	32.0	32.0	32.0	32.0
3	34.91965025921979	37.0	32.0	37.0	32.0	37.0
4	35.502972130540336	37.0	37.0	37.0	32.0	37.0
5	35.843172081690525	37.0	37.0	37.0	32.0	37.0
6	39.01392293834129	41.0	41.0	41.0	37.0	41.0
7	39.21605977072594	41.0	41.0	41.0	37.0	41.0
8	39.3920006515788	41.0	41.0	41.0	37.0	41.0
9	39.36525480252134	41.0	41.0	41.0	37.0	41.0
10-14	39.37931537804572	41.0	41.0	41.0	37.0	41.0
15-19	39.29893358859395	41.0	41.0	41.0	37.0	41.0
20-24	39.27214904855681	41.0	41.0	41.0	37.0	41.0
25-29	39.11705955438578	41.0	41.0	41.0	37.0	41.0
30-34	39.104327989360776	41.0	41.0	41.0	37.0	41.0
35-39	39.0463032508563	41.0	41.0	41.0	37.0	41.0
40-44	38.93881118778656	41.0	41.0	41.0	35.0	41.0
45-49	38.86256740744992	41.0	41.0	41.0	32.0	41.0
50-54	38.786156415844026	41.0	41.0	41.0	32.0	41.0
55-59	38.65575750663382	41.0	41.0	41.0	32.0	41.0
60-64	38.58867552515516	41.0	41.0	41.0	32.0	41.0
65-69	38.459940123406454	41.0	41.0	41.0	32.0	41.0
70-74	38.34486380965861	41.0	41.0	41.0	32.0	41.0
75-79	37.85626366167655	41.0	37.8	41.0	31.0	41.0
80-84	38.52677203221742	41.0	41.0	41.0	32.0	41.0
85-89	38.45297570054838	41.0	41.0	41.0	32.0	41.0
90-94	38.34979318373596	41.0	41.0	41.0	32.0	41.0
95-99	38.17673221790623	41.0	38.6	41.0	32.0	41.0
100-104	37.93588263692749	41.0	37.0	41.0	32.0	41.0
105-109	37.74615396172514	41.0	37.0	41.0	29.0	41.0
110-114	37.49439435243799	41.0	37.0	41.0	27.0	41.0
115-119	37.10800467436154	41.0	37.0	41.0	27.0	41.0
120-124	36.738203714287536	41.0	37.0	41.0	27.0	41.0
125-129	36.35894109328577	41.0	37.0	41.0	23.0	41.0
130-134	35.905317814209894	41.0	33.0	41.0	22.0	41.0
135-139	35.36662945330595	41.0	32.0	41.0	22.0	41.0
140-144	34.82776349903768	41.0	32.0	41.0	22.0	41.0
145-149	34.09639468259977	37.8	30.0	41.0	12.0	41.0
150	29.21239231425411	32.0	22.0	37.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	25.0
13	199.0
14	793.0
15	3364.0
16	9402.0
17	19237.0
18	29420.0
19	40975.0
20	54534.0
21	69232.0
22	85687.0
23	104120.0
24	123841.0
25	146496.0
26	171483.0
27	199870.0
28	233091.0
29	270711.0
30	314786.0
31	368434.0
32	433134.0
33	515030.0
34	620150.0
35	766560.0
36	977058.0
37	1319679.0
38	2006585.0
39	3891783.0
40	8722870.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.10409005883529	22.715215551223604	7.0791500138162515	33.101544376124856
2	26.629527273661154	26.185105140374425	30.16212586790003	17.023241718064394
3	21.360853575666564	26.16133524533816	27.857984475325804	24.61982670366947
4	23.67783298511607	34.62702672380106	21.025412363837916	20.669727927244956
5	26.64935534917385	35.62742168508814	18.884696857356296	18.838526108381718
6	22.262979491036397	36.41462161798719	19.292053683059848	22.03034520791657
7	21.12162721221639	20.22746557404897	34.444394208292664	24.20651300544197
8	20.11837448951792	23.512882333325628	26.93329890000494	29.435444277151518
9	21.514924424441443	21.943060255549316	29.360709007783825	27.181306312225416
10-14	24.610224242499907	27.01688295979492	23.674880572899546	24.69801222480563
15-19	24.487184006274067	26.496277636820793	24.252361332293003	24.76417702461214
20-24	24.268767330987664	26.78481810364725	24.457955873992514	24.48845869137257
25-29	24.17559679473782	26.51554360739224	24.67291527707341	24.635944320796526
30-34	24.111134197432406	26.624294036285956	24.72292708687067	24.54164467941097
35-39	24.007281545675692	26.759072874270128	24.62584248387344	24.60780309618074
40-44	24.02475666937604	26.570277895277123	24.8179202595549	24.587045175791943
45-49	23.939200504773023	26.535103350776634	24.798005482701015	24.727690661749328
50-54	24.011185649901556	26.639920732868937	24.760285640165083	24.58860797706442
55-59	24.116113651000422	26.507391533439467	24.69797987892644	24.678514936633672
60-64	23.999220186011275	26.5092418107228	24.94176489905077	24.549773104215156
65-69	24.191680643041824	26.519714852363208	24.971444879868667	24.317159624726305
70-74	24.230685822163903	26.573379967317983	24.863564671711753	24.332369538806358
75-79	24.132087117818678	26.640387505449347	24.845149561407062	24.382375815324913
80-84	24.00333641680241	26.779043741304342	24.816578709420508	24.40104113247274
85-89	24.111833177096486	26.68359138190325	24.643251807825376	24.561323633174894
90-94	24.029953470096558	26.85844545558985	24.71576757604477	24.395833498268825
95-99	23.990353325599887	26.97772598413277	24.616411650608054	24.415509039659288
100-104	24.270139578568756	27.034765819254098	24.41990443891879	24.275190163258358
105-109	24.410374958141155	27.290153820632142	24.228868785396283	24.07060243583042
110-114	24.52437738390418	27.270998188404228	24.160225544245527	24.04439888344606
115-119	24.810455713876504	27.35252534244445	24.024485051257766	23.81253389242128
120-124	25.082839903155733	27.434756636832454	23.853184807237998	23.629218652773815
125-129	25.60042796629178	27.648887535286388	23.547402500663814	23.203281997758022
130-134	26.251059622025714	27.548149268572526	23.347975524097585	22.852815585304175
135-139	26.714554070791085	27.587631254460167	23.317707438546677	22.380107236202075
140-144	27.108746847487335	27.643323091278866	23.090381126462756	22.157548934771047
145-149	27.766158266237685	27.332545024555465	23.14571223464886	21.755584474557992
150	27.706428701240483	27.24788664245651	23.346650921426352	21.699033734876654
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	123.0
1	99.0
2	74.5
3	90.5
4	120.5
5	131.5
6	142.5
7	162.5
8	186.5
9	230.5
10	275.0
11	318.5
12	372.0
13	426.0
14	535.5
15	669.5
16	809.5
17	1075.5
18	1532.0
19	2216.5
20	3378.0
21	5192.0
22	7974.0
23	11364.5
24	16326.0
25	22931.5
26	31835.0
27	45355.5
28	62045.0
29	85483.0
30	108872.5
31	132224.5
32	166483.0
33	211936.5
34	255030.0
35	288124.5
36	329843.0
37	379287.5
38	412000.0
39	432988.0
40	458135.0
41	510815.0
42	581681.5
43	655435.5
44	711582.0
45	769333.5
46	843569.0
47	896881.0
48	947228.5
49	1003445.0
50	1085123.0
51	1111146.0
52	1073193.5
53	1029536.5
54	953990.0
55	894404.0
56	831232.5
57	742339.5
58	659975.0
59	566649.0
60	472990.0
61	388285.5
62	309364.5
63	249604.0
64	192784.0
65	140606.5
66	103528.5
67	77926.5
68	61120.0
69	46601.0
70	33735.0
71	24366.0
72	18447.0
73	12667.5
74	7930.0
75	5217.0
76	3372.5
77	2063.0
78	1261.5
79	858.0
80	565.0
81	361.0
82	241.0
83	161.0
84	109.0
85	87.0
86	62.5
87	44.0
88	40.5
89	30.5
90	25.5
91	24.5
92	24.0
93	18.5
94	20.5
95	21.0
96	13.0
97	10.5
98	12.0
99	14.0
100	23.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.4407296566173227
2	0.021266549545594954
3	0.0
4	0.0
5	4.2328434134933097E-4
6	0.0
7	4.139813887922028E-4
8	0.0
9	0.0
10-14	7.637724049402212E-4
15-19	1.4326546937977355E-4
20-24	9.72158542219892E-4
25-29	2.502494237867473E-4
30-34	0.0022857354432863873
35-39	1.7954698435257335E-4
40-44	3.9816636944508497E-4
45-49	4.474720179978642E-4
50-54	2.6978562415671643E-5
55-59	0.0019247808840698148
60-64	4.223540460936182E-4
65-69	6.037616209576171E-4
70-74	5.749224680305198E-4
75-79	4.521234942764282E-4
80-84	1.767560985854349E-4
85-89	9.675070659413279E-4
90-94	0.0011498449360610396
95-99	3.479304256365929E-4
100-104	2.3257381392820383E-4
105-109	5.051503238520587E-4
110-114	2.1582849932537314E-4
115-119	6.139948687704581E-5
120-124	1.6187137449402987E-4
125-129	1.646622602611683E-4
130-134	3.870028263765311E-4
135-139	8.223810060501287E-4
140-144	3.0141566285095216E-4
145-149	1.7582580332972208E-4
150	0.0019257111793255276
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.1498551E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.94959118518874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.61373759483959	24.99156296958563
2	12.220070472355603	8.297327935813378
3	4.620576145368396	4.706000135258768
4	2.4236396220797967	3.291262973993377
5	1.4536070885492898	2.4674683200070415
6	0.9815915583504994	1.9994779270099072
7	0.7372675400589853	1.7520952105378618
8	0.5008402523901245	1.360265745418518
9	0.3972147165723688	1.2136749516335281
>10	2.5623151510619597	16.607023836006494
>50	0.25685166573708645	6.060347273207732
>100	0.1985847786811472	13.498267814385892
>500	0.020640265023391304	4.82195473808181
>1k	0.012552756395351798	7.701882999170347
>5k	4.8203739546357133E-4	1.093959906606943
>10k+	2.835514090962172E-5	0.13742726328279997
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.395442883569223E-5	0.0	0.0	0.0	0.0
2	1.8605905114256306E-5	0.0	0.0	0.0	0.0
3	2.3257381392820382E-5	0.0	0.0	0.0	4.6514762785640765E-6
4	6.977214417846114E-5	9.302952557128153E-6	0.0	0.0	4.6514762785640765E-6
5	9.302952557128153E-5	9.302952557128153E-6	0.0	4.6514762785640765E-6	4.6514762785640765E-6
6	1.348928120783582E-4	9.302952557128153E-6	0.0	4.6514762785640765E-6	4.6514762785640765E-6
7	1.5349871719261453E-4	9.302952557128153E-6	0.0	4.6514762785640765E-6	4.6514762785640765E-6
8	1.5349871719261453E-4	1.395442883569223E-5	0.0	9.302952557128153E-6	4.6514762785640765E-6
9	2.4187676648533196E-4	1.395442883569223E-5	0.0	9.302952557128153E-6	4.6514762785640765E-6
10-11	3.232776013602033E-4	1.395442883569223E-5	0.0	9.302952557128153E-6	9.302952557128153E-6
12-13	4.697991041349717E-4	1.395442883569223E-5	0.0	1.395442883569223E-5	9.302952557128153E-6
14-15	5.884117492383557E-4	1.395442883569223E-5	0.0	1.395442883569223E-5	1.395442883569223E-5
16-17	6.907442273667653E-4	1.395442883569223E-5	0.0	1.395442883569223E-5	1.395442883569223E-5
18-19	8.070311343308672E-4	1.395442883569223E-5	0.0	1.8605905114256306E-5	1.6280166974974266E-5
20-21	9.093636124592769E-4	1.395442883569223E-5	0.0	1.8605905114256306E-5	1.8605905114256306E-5
22-23	0.0010163475668662507	1.395442883569223E-5	0.0	2.0931643253538346E-5	1.8605905114256306E-5
24-25	0.0011163543068553783	1.395442883569223E-5	0.0	2.3257381392820382E-5	1.8605905114256306E-5
26-27	0.0012326412138194802	1.395442883569223E-5	0.0	2.3257381392820382E-5	1.8605905114256306E-5
28-29	0.0013582310733407103	1.395442883569223E-5	0.0	2.3257381392820382E-5	1.8605905114256306E-5
30-31	0.0015675475058760937	2.0931643253538346E-5	0.0	2.790885767138446E-5	2.0931643253538346E-5
32-33	0.00183733313003281	2.3257381392820382E-5	0.0	3.488607208923057E-5	2.3257381392820382E-5
34-35	0.002139679088139475	2.558311953210242E-5	0.0	4.1863286507076685E-5	2.3257381392820382E-5
36-37	0.002562963429488806	2.790885767138446E-5	0.0	4.88405009249228E-5	2.3257381392820382E-5
38-39	0.0030188081047880858	4.1863286507076685E-5	0.0	5.116623906420484E-5	2.3257381392820382E-5
40-41	0.003470001303808801	4.1863286507076685E-5	0.0	5.116623906420484E-5	2.3257381392820382E-5
42-43	0.004149116840479156	4.1863286507076685E-5	0.0	5.116623906420484E-5	2.3257381392820382E-5
44-45	0.004858466972960178	4.1863286507076685E-5	0.0	5.116623906420484E-5	2.3257381392820382E-5
46-47	0.005746898942165917	4.1863286507076685E-5	0.0	6.046919162133299E-5	2.3257381392820382E-5
48-49	0.006940002607617602	4.1863286507076685E-5	0.0	6.046919162133299E-5	2.3257381392820382E-5
50-51	0.008314513847933287	4.1863286507076685E-5	0.0	6.046919162133299E-5	2.3257381392820382E-5
52-53	0.009977416617519944	4.1863286507076685E-5	0.0	6.512066789989706E-5	2.3257381392820382E-5
54-55	0.011910105011263317	4.1863286507076685E-5	0.0	6.977214417846114E-5	2.558311953210242E-5
56-57	0.014193979864038279	4.1863286507076685E-5	0.0	6.977214417846114E-5	2.790885767138446E-5
58-59	0.01705696351349447	4.4189024646358725E-5	0.0	6.977214417846114E-5	2.790885767138446E-5
60-61	0.020450215458706962	4.88405009249228E-5	0.0	6.977214417846114E-5	2.790885767138446E-5
62-63	0.02472492215870735	5.116623906420484E-5	0.0	7.442362045702522E-5	2.790885767138446E-5
64-65	0.0301950582622987	5.116623906420484E-5	0.0	7.442362045702522E-5	2.790885767138446E-5
66-67	0.03675596555321333	5.116623906420484E-5	0.0	7.442362045702522E-5	2.790885767138446E-5
68-69	0.04499838151882887	5.116623906420484E-5	0.0	7.442362045702522E-5	2.790885767138446E-5
70-71	0.05498510108890595	5.116623906420484E-5	0.0	7.907509673558929E-5	2.790885767138446E-5
72-73	0.06824413422095285	5.116623906420484E-5	0.0	8.837804929271745E-5	2.790885767138446E-5
74-75	0.08448011217128076	5.116623906420484E-5	0.0	9.535526371056357E-5	2.790885767138446E-5
76-77	0.10432098423749582	5.349197720348688E-5	0.0	1.0698395440697376E-4	3.0234595810666496E-5
78-79	0.12880170389157855	5.581771534276892E-5	0.0	1.1163543068553784E-4	3.256033394994853E-5
80-81	0.15890838410458452	5.581771534276892E-5	0.0	1.1396116882481986E-4	3.256033394994853E-5
82-83	0.1980017164877763	5.581771534276892E-5	0.0	1.162869069641019E-4	3.256033394994853E-5
84-85	0.24665848409969585	5.581771534276892E-5	0.0	1.2093838324266598E-4	3.721181022851261E-5
86-87	0.30840450595949465	5.581771534276892E-5	0.0	1.2093838324266598E-4	3.721181022851261E-5
88-89	0.3834979390006331	5.581771534276892E-5	0.0	1.2558985952123005E-4	3.721181022851261E-5
90-91	0.47432266481587526	5.814345348205096E-5	0.0	1.395442883569223E-4	3.721181022851261E-5
92-93	0.5876837932007604	6.512066789989706E-5	0.0	1.4419576463548637E-4	4.1863286507076685E-5
94-95	0.7252488784011537	6.512066789989706E-5	0.0	1.5582445533189655E-4	4.1863286507076685E-5
96-97	0.8910205157547595	6.74464060391791E-5	0.0	1.6745314602830674E-4	4.1863286507076685E-5
98-99	1.089552500538292	6.977214417846114E-5	0.0	1.6745314602830674E-4	4.1863286507076685E-5
100-101	1.3277685551923941	6.977214417846114E-5	0.0	1.7210462230687082E-4	4.1863286507076685E-5
102-103	1.6079665089986763	6.977214417846114E-5	0.0	2.0466495625681935E-4	4.1863286507076685E-5
104-105	1.940591251940654	7.442362045702522E-5	0.0	2.0931643253538343E-4	4.1863286507076685E-5
106-107	2.3243892111612547	7.907509673558929E-5	0.0	2.139679088139475E-4	4.1863286507076685E-5
108-109	2.7699145863365393	8.140083487487133E-5	0.0	2.186193850925116E-4	4.1863286507076685E-5
110-111	3.2696738491817428	8.372657301415337E-5	0.0	2.186193850925116E-4	4.1863286507076685E-5
112-113	3.8319070899243393	8.372657301415337E-5	0.0	2.2327086137107567E-4	4.1863286507076685E-5
114-115	4.467784828847302	8.837804929271745E-5	0.0	2.2792233764963972E-4	4.1863286507076685E-5
116-117	5.178835075908139	9.070378743199949E-5	0.0	2.3489955206748586E-4	4.1863286507076685E-5
118-119	5.956843323998905	9.302952557128153E-5	0.0	2.3722529020676788E-4	4.1863286507076685E-5
120-121	6.793113638216827	9.302952557128153E-5	0.0	2.395510283460499E-4	4.1863286507076685E-5
122-123	7.701400433917616	9.302952557128153E-5	0.0	2.4187676648533196E-4	4.1863286507076685E-5
124-125	8.675640511772166	9.535526371056357E-5	0.0	2.4187676648533196E-4	4.1863286507076685E-5
126-127	9.71445936054016	9.76810018498456E-5	0.0	2.4187676648533196E-4	4.1863286507076685E-5
128-129	10.821036264257994	1.0233247812840968E-4	0.0	2.44202504624614E-4	4.1863286507076685E-5
130-131	11.975946657986391	1.0233247812840968E-4	0.0	2.511797190424601E-4	4.6514762785640765E-5
132-133	13.17166445310663	1.0233247812840968E-4	0.0	2.5815693346030623E-4	4.6514762785640765E-5
134-135	14.414948244651466	1.0233247812840968E-4	0.0	2.7211136229599844E-4	4.6514762785640765E-5
136-137	15.68660604149554	1.0233247812840968E-4	0.0	2.744371004352805E-4	4.6514762785640765E-5
138-139	16.982367788415136	1.0930969254625578E-4	0.0	2.7676283857456255E-4	4.88405009249228E-5
>>END_MODULE
