##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261782_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21498551
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.69192719081393	32.0	32.0	32.0	32.0	32.0
2	31.525598957808832	32.0	32.0	32.0	32.0	32.0
3	35.1661552446023	37.0	32.0	37.0	32.0	37.0
4	36.04982247408209	37.0	37.0	37.0	32.0	37.0
5	36.30658196452403	37.0	37.0	37.0	37.0	37.0
6	39.61008548901738	41.0	41.0	41.0	37.0	41.0
7	39.77939169016554	41.0	41.0	41.0	37.0	41.0
8	39.947878115134365	41.0	41.0	41.0	37.0	41.0
9	40.04199915612917	41.0	41.0	41.0	37.0	41.0
10-14	40.05776787468142	41.0	41.0	41.0	37.0	41.0
15-19	40.00062868423087	41.0	41.0	41.0	37.0	41.0
20-24	39.94945735645162	41.0	41.0	41.0	37.0	41.0
25-29	39.86174702657867	41.0	41.0	41.0	37.0	41.0
30-34	39.81655394356578	41.0	41.0	41.0	37.0	41.0
35-39	39.72486311286747	41.0	41.0	41.0	37.0	41.0
40-44	39.599722018474644	41.0	41.0	41.0	37.0	41.0
45-49	39.573953751580746	41.0	41.0	41.0	37.0	41.0
50-54	39.61201290263702	41.0	41.0	41.0	37.0	41.0
55-59	39.56031253455175	41.0	41.0	41.0	37.0	41.0
60-64	39.50586856760718	41.0	41.0	41.0	37.0	41.0
65-69	39.48323572132838	41.0	41.0	41.0	37.0	41.0
70-74	39.43435695735959	41.0	41.0	41.0	37.0	41.0
75-79	39.07533409112084	41.0	40.2	41.0	35.0	41.0
80-84	39.488429262046544	41.0	41.0	41.0	37.0	41.0
85-89	39.468280266888684	41.0	41.0	41.0	37.0	41.0
90-94	39.428165954068255	41.0	41.0	41.0	37.0	41.0
95-99	39.39363333835848	41.0	41.0	41.0	37.0	41.0
100-104	39.30374835029579	41.0	41.0	41.0	37.0	41.0
105-109	39.181874192358364	41.0	41.0	41.0	37.0	41.0
110-114	39.09327069531337	41.0	41.0	41.0	37.0	41.0
115-119	38.90186632578168	41.0	41.0	41.0	34.0	41.0
120-124	38.73454499328815	41.0	41.0	41.0	32.0	41.0
125-129	38.64365647712723	41.0	41.0	41.0	32.0	41.0
130-134	38.388529450194106	41.0	37.0	41.0	32.0	41.0
135-139	38.167724829454784	41.0	37.0	41.0	32.0	41.0
140-144	37.855059552618215	41.0	37.0	41.0	32.0	41.0
145-149	37.55143802947463	41.0	37.0	41.0	29.0	41.0
150	34.425199865795605	37.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	10.0
16	52.0
17	140.0
18	392.0
19	1000.0
20	2554.0
21	5083.0
22	9208.0
23	15856.0
24	26059.0
25	40443.0
26	60243.0
27	85865.0
28	117680.0
29	153650.0
30	196310.0
31	244253.0
32	299313.0
33	363677.0
34	443769.0
35	548176.0
36	695510.0
37	932017.0
38	1383511.0
39	2796261.0
40	1.3077516E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.55830730414328	10.991204074196448	4.61750252240825	34.832986099252025
2	25.634230003901404	12.414550142841762	40.326762472493236	21.624457380763605
3	23.747200450858294	15.808800323333418	25.59945086531646	34.84454836049183
4	26.242089525010314	25.144685332513806	23.991119215429915	24.62210592704597
5	27.8662082853863	28.56928822784382	24.845786118329556	18.71871736844032
6	22.938037770310927	29.72623372012666	26.10064168667247	21.235086822889937
7	18.16430325932199	22.31836461908526	42.96443978945372	16.552892332139034
8	19.486676102031247	20.96909694053334	34.40153710824511	25.142689849190305
9	19.74034436088274	19.31447845019881	36.50535331427685	24.439823874641597
10-14	24.63159283129997	25.459324519790805	26.851275077020826	23.057807571888407
15-19	24.909588520843986	24.474241793008645	27.164611189311245	23.451558496836125
20-24	24.57933131458594	24.706811564901287	27.221499790930043	23.49235732958273
25-29	24.412998242431023	24.870704834698508	27.190790775797968	23.525506147072498
30-34	24.440651688543095	24.974216606110424	26.738109513491608	23.847022191854872
35-39	24.655561098474365	24.89517966759803	26.82874333265136	23.620515901276242
40-44	24.53053623807006	24.742749460638333	27.068779826393026	23.657934474898585
45-49	24.50388527664086	24.58772737128175	27.170124729000772	23.73826262307662
50-54	24.274066743094625	25.04900198274144	26.947878643225426	23.729052630938508
55-59	24.626291392785607	24.849334194157198	26.801620613585303	23.722753799471892
60-64	24.186678942912526	24.651218933253215	27.10624204215108	24.05586008168318
65-69	24.464686779901292	25.12438017832883	26.71124031613529	23.699692725634588
70-74	24.524496016646623	24.89869650017354	26.71584459933537	23.860962883844472
75-79	24.412705290118055	24.84932715243945	26.713691852628703	24.024275704813796
80-84	24.3455858118979	24.85046019250022	26.837423201344922	23.96653079425696
85-89	24.720439522062133	24.750999874735115	26.641752614363746	23.886807988839006
90-94	24.48159674009156	24.89350052331527	26.692608412742324	23.932294323850844
95-99	24.659049295144403	24.969436901066803	26.59321816807615	23.77829563571264
100-104	24.55885822683099	24.981251217645198	26.54199908655795	23.917891468965866
105-109	24.70012491709623	25.055063585514738	26.236169075581856	24.008642421807178
110-114	24.90315547608295	25.159263791462504	26.156578465173148	23.781002267281398
115-119	24.76614161092123	25.55900442016335	25.868345465572425	23.806508503342993
120-124	24.97366983512434	25.54179353572788	25.62181271413333	23.86272391501445
125-129	24.848946566025436	25.806357982467688	25.358781122999474	23.9859143285074
130-134	24.89550134579817	25.81924594926947	25.1357589870573	24.14949371787506
135-139	24.88391638541338	26.14215745686421	24.701561474395497	24.272364683326913
140-144	24.878799161653248	26.24901507820528	24.51090679396093	24.361278966180535
145-149	24.68558953634743	26.37192237858107	24.34187929039504	24.600608794676464
150	24.46650253113219	26.173226482895895	24.3509759728854	25.009295013086515
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	139.0
1	108.0
2	69.0
3	66.0
4	75.0
5	89.5
6	106.0
7	118.5
8	129.5
9	140.5
10	165.5
11	201.5
12	269.5
13	305.0
14	342.5
15	474.5
16	636.5
17	931.5
18	1356.0
19	1965.5
20	3112.5
21	5186.0
22	7983.0
23	11145.5
24	16181.0
25	23808.5
26	33370.0
27	45573.5
28	62139.5
29	85108.0
30	109766.5
31	133399.5
32	167657.0
33	210357.0
34	256096.0
35	303014.5
36	341774.5
37	387195.5
38	428117.5
39	443153.0
40	462109.5
41	518478.5
42	583355.0
43	644696.0
44	712985.5
45	795200.0
46	864275.0
47	909352.0
48	955577.5
49	1021093.0
50	1112685.0
51	1133453.0
52	1096846.0
53	1048015.0
54	956372.5
55	882115.5
56	807796.0
57	717448.0
58	649523.5
59	575019.0
60	477444.5
61	378104.5
62	296654.0
63	227575.5
64	172524.5
65	126744.0
66	88311.0
67	64961.5
68	46777.0
69	30682.0
70	20816.0
71	13850.5
72	9576.5
73	6388.0
74	3899.0
75	2387.0
76	1612.5
77	955.5
78	459.5
79	266.5
80	173.5
81	102.5
82	55.0
83	30.5
84	18.5
85	8.5
86	6.5
87	3.0
88	2.0
89	1.0
90	0.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.398024452903826
2	0.20811170017923536
3	0.0
4	0.0
5	0.0
6	0.006767897985310732
7	0.0
8	0.0
9	0.0
10-14	6.69812584113227E-5
15-19	4.093299125136387E-5
20-24	2.4187676648533195E-5
25-29	4.930564855277921E-4
30-34	1.097748401741122E-4
35-39	2.3350410918391662E-4
40-44	0.0012447350521437469
45-49	0.0024159767790861812
50-54	6.288795928618631E-4
55-59	3.5537278768229543E-4
60-64	5.674801059848173E-4
65-69	5.088715048749099E-4
70-74	8.474989779543746E-4
75-79	3.9816636944508497E-4
80-84	0.09220156279369712
85-89	5.023594380849202E-4
90-94	0.001067048658302599
95-99	3.563030829380082E-4
100-104	4.102602077693515E-4
105-109	6.167857545375965E-4
110-114	0.0017740730526443387
115-119	0.08366796441304347
120-124	9.526223418499228E-4
125-129	7.684238812187854E-4
130-134	0.0017647701000872106
135-139	0.001182405270010988
140-144	6.679519936018014E-4
145-149	0.001248456233166598
150	0.0021582849932537313
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.1498551E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.67655161343935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.53401382885923	28.45861170593257
2	11.82454631633511	8.910162591858079
3	4.231934640699457	4.783341117450449
4	2.1295089865329415	3.2093022096956516
5	1.2977411348883134	2.444720542475145
6	0.8735862639952876	1.9748230778526066
7	0.6270866133979331	1.653852280904888
8	0.46404691202127923	1.3986949945461502
9	0.3607775344278501	1.2233568057156257
>10	2.253507378067076	16.178705460370026
>50	0.21509301577222317	5.630020131212284
>100	0.1617067010680889	11.873412665109734
>500	0.0167756347602908	4.321199393242464
>1k	0.009099441409993914	6.294535701678948
>5k	5.372245807019881E-4	1.4238899520641866
>10k+	3.837318433585597E-5	0.22137136989124756
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG	22328	0.1038581623477787	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6514762785640765E-5	0.0	0.0	0.0	4.6514762785640765E-6
2	6.046919162133299E-5	4.6514762785640765E-6	0.0	1.395442883569223E-5	4.6514762785640765E-6
3	8.837804929271745E-5	4.6514762785640765E-6	0.0	1.395442883569223E-5	1.8605905114256306E-5
4	1.4884724091405045E-4	4.6514762785640765E-6	0.0	1.8605905114256306E-5	1.8605905114256306E-5
5	1.8140757486399898E-4	4.6514762785640765E-6	0.0	1.8605905114256306E-5	1.8605905114256306E-5
6	2.2792233764963972E-4	4.6514762785640765E-6	0.0	1.8605905114256306E-5	1.8605905114256306E-5
7	2.697856241567164E-4	4.6514762785640765E-6	0.0	1.8605905114256306E-5	1.8605905114256306E-5
8	2.744371004352805E-4	4.6514762785640765E-6	0.0	2.3257381392820382E-5	2.3257381392820382E-5
9	3.721181022851261E-4	4.6514762785640765E-6	0.0	3.256033394994853E-5	3.256033394994853E-5
10-11	4.83753532970664E-4	4.6514762785640765E-6	0.0	4.651476278564076E-5	3.721181022851261E-5
12-13	6.372522501632784E-4	4.6514762785640765E-6	0.0	5.116623906420484E-5	4.6514762785640765E-5
14-15	7.488876808488163E-4	4.6514762785640765E-6	0.0	5.116623906420484E-5	5.581771534276892E-5
16-17	8.395914682808158E-4	4.6514762785640765E-6	4.6514762785640765E-6	5.814345348205096E-5	5.581771534276892E-5
18-19	9.395982082699434E-4	4.6514762785640765E-6	4.6514762785640765E-6	6.977214417846114E-5	6.279492976061502E-5
20-21	0.0010233247812840968	4.6514762785640765E-6	4.6514762785640765E-6	7.209788231774318E-5	6.977214417846114E-5
22-23	0.0010907711873232757	9.302952557128153E-6	4.6514762785640765E-6	8.837804929271745E-5	6.977214417846114E-5
24-25	0.0011558918552231731	1.395442883569223E-5	4.6514762785640765E-6	1.0000673998912765E-4	6.977214417846114E-5
26-27	0.001214035308705224	1.395442883569223E-5	4.6514762785640765E-6	1.0698395440697376E-4	7.442362045702522E-5
28-29	0.001276830238465839	1.395442883569223E-5	4.6514762785640765E-6	1.0698395440697376E-4	1.0233247812840968E-4
30-31	0.0013884656691513769	1.395442883569223E-5	4.6514762785640765E-6	1.0698395440697376E-4	1.0233247812840968E-4
32-33	0.0015094040523940429	1.395442883569223E-5	4.6514762785640765E-6	1.0698395440697376E-4	1.1396116882481986E-4
34-35	0.0016977888416758878	1.6280166974974266E-5	4.6514762785640765E-6	1.0698395440697376E-4	1.162869069641019E-4
36-37	0.0019699002039718864	2.3257381392820382E-5	4.6514762785640765E-6	1.162869069641019E-4	1.279155976605121E-4
38-39	0.002307132234167782	2.3257381392820382E-5	4.6514762785640765E-6	1.1861264510338394E-4	1.4652150277476842E-4
40-41	0.0026839018127314722	2.3257381392820382E-5	4.6514762785640765E-6	1.279155976605121E-4	1.534987171926145E-4
42-43	0.003221147322905623	2.3257381392820382E-5	4.6514762785640765E-6	1.3024133579979413E-4	1.6280166974974266E-4
44-45	0.0037583928330797737	2.3257381392820382E-5	4.6514762785640765E-6	1.348928120783582E-4	1.651274078890247E-4
46-47	0.004486348870675051	2.3257381392820382E-5	4.6514762785640765E-6	1.395442883569223E-4	1.697788841675888E-4
48-49	0.005516650866376994	2.3257381392820382E-5	4.6514762785640765E-6	1.4419576463548637E-4	1.7443036044615285E-4
50-51	0.006723708960664372	2.3257381392820382E-5	4.6514762785640765E-6	1.4419576463548637E-4	1.8605905114256306E-4
52-53	0.008256370394451235	2.3257381392820382E-5	4.6514762785640765E-6	1.5349871719261453E-4	1.953620036996912E-4
54-55	0.01006579466681266	2.3257381392820382E-5	4.6514762785640765E-6	1.5349871719261453E-4	2.0233921811753733E-4
56-57	0.012170587682862905	2.790885767138446E-5	4.6514762785640765E-6	1.581501934711786E-4	2.0931643253538343E-4
58-59	0.014905655734658583	3.721181022851261E-5	4.6514762785640765E-6	1.651274078890247E-4	2.0931643253538343E-4
60-61	0.018136106010121333	4.1863286507076685E-5	4.6514762785640765E-6	1.6745314602830674E-4	2.0931643253538343E-4
62-63	0.02222707939711844	4.6514762785640765E-5	4.6514762785640765E-6	1.6745314602830674E-4	2.1164217067466549E-4
64-65	0.02756929990304928	4.6514762785640765E-5	4.6514762785640765E-6	1.6745314602830674E-4	2.2094512323179365E-4
66-67	0.03399764012002483	5.116623906420484E-5	4.6514762785640765E-6	1.7210462230687082E-4	2.2792233764963972E-4
68-69	0.04201911096240858	5.116623906420484E-5	6.977214417846115E-6	1.83733313003281E-4	2.325738139282038E-4
70-71	0.051919778221332216	5.116623906420484E-5	9.302952557128153E-6	1.9303626556040917E-4	2.3722529020676788E-4
72-73	0.06510903920920066	5.116623906420484E-5	9.302952557128153E-6	1.953620036996912E-4	2.3722529020676788E-4
74-75	0.0812612905865144	5.116623906420484E-5	9.302952557128153E-6	1.953620036996912E-4	2.511797190424601E-4
76-77	0.1010602993662224	5.116623906420484E-5	9.302952557128153E-6	1.953620036996912E-4	2.558311953210242E-4
78-79	0.12548287556682308	5.581771534276892E-5	1.1628690696410191E-5	2.0466495625681935E-4	2.5815693346030623E-4
80-81	0.1553383760607866	5.581771534276892E-5	1.395442883569223E-5	2.0466495625681935E-4	2.6048267159958826E-4
82-83	0.1944689202542069	5.581771534276892E-5	1.395442883569223E-5	2.0466495625681935E-4	2.6048267159958826E-4
84-85	0.24352106334980436	5.581771534276892E-5	1.395442883569223E-5	2.0466495625681935E-4	2.6048267159958826E-4
86-87	0.30540197802168156	5.581771534276892E-5	1.395442883569223E-5	2.1629364695322954E-4	2.6513414787815236E-4
88-89	0.38082101440231947	5.581771534276892E-5	1.395442883569223E-5	2.255965995103577E-4	2.6513414787815236E-4
90-91	0.47271325402349207	5.581771534276892E-5	1.395442883569223E-5	2.2792233764963972E-4	2.6513414787815236E-4
92-93	0.5873930759333501	5.814345348205096E-5	1.395442883569223E-5	2.2792233764963972E-4	2.697856241567164E-4
94-95	0.7261047500364095	6.046919162133299E-5	1.395442883569223E-5	2.325738139282038E-4	3.000202199673829E-4
96-97	0.8933043906075344	6.046919162133299E-5	1.395442883569223E-5	2.44202504624614E-4	3.0234595810666495E-4
98-99	1.0932783330374218	6.046919162133299E-5	1.395442883569223E-5	2.4652824276389604E-4	3.116489106637931E-4
100-101	1.332368865231894	6.046919162133299E-5	1.395442883569223E-5	2.4885398090317807E-4	3.2095186322092127E-4
102-103	1.613529674627839	6.046919162133299E-5	1.395442883569223E-5	2.511797190424601E-4	3.256033394994853E-4
104-105	1.948019659557521	6.046919162133299E-5	1.395442883569223E-5	2.6280840973887034E-4	3.279290776387674E-4
106-107	2.3351341213647374	6.046919162133299E-5	1.395442883569223E-5	2.7211136229599844E-4	3.3025481577804943E-4
108-109	2.782608465100741	6.046919162133299E-5	1.395442883569223E-5	2.7676283857456255E-4	3.3025481577804943E-4
110-111	3.2849376685898504	6.279492976061502E-5	1.395442883569223E-5	2.790885767138446E-4	3.3025481577804943E-4
112-113	3.8508153409967023	6.512066789989706E-5	1.395442883569223E-5	2.8374005299240863E-4	3.3025481577804943E-4
114-115	4.492367880979513	6.512066789989706E-5	1.395442883569223E-5	2.930430055495368E-4	3.349062920566135E-4
116-117	5.210113928143343	6.512066789989706E-5	1.395442883569223E-5	2.930430055495368E-4	3.349062920566135E-4
118-119	5.994769135836178	6.512066789989706E-5	1.395442883569223E-5	3.0234595810666495E-4	3.349062920566135E-4
120-121	6.841486665775754	6.74464060391791E-5	1.395442883569223E-5	3.0234595810666495E-4	3.349062920566135E-4
122-123	7.761116086381822	6.977214417846114E-5	1.395442883569223E-5	3.0234595810666495E-4	3.372320301958955E-4
124-125	8.74792910461733	6.977214417846114E-5	1.395442883569223E-5	3.0234595810666495E-4	3.4420924461374164E-4
126-127	9.802253649559916	6.977214417846114E-5	1.395442883569223E-5	3.0234595810666495E-4	3.535121971708698E-4
128-129	10.926059621413554	6.977214417846114E-5	1.395442883569223E-5	3.0234595810666495E-4	3.535121971708698E-4
130-131	12.100527147155173	6.977214417846114E-5	1.395442883569223E-5	3.0234595810666495E-4	3.581636734494339E-4
132-133	13.319293007235697	7.209788231774318E-5	1.395442883569223E-5	3.0234595810666495E-4	3.67466626006562E-4
134-135	14.591192680846259	8.372657301415337E-5	1.395442883569223E-5	3.0234595810666495E-4	3.7444384042440815E-4
136-137	15.893608364582338	8.837804929271745E-5	1.395442883569223E-5	3.0234595810666495E-4	4.0002695995651054E-4
138-139	17.22698892590482	9.535526371056357E-5	1.6280166974974266E-5	3.0467169624594703E-4	4.2328434134933097E-4
>>END_MODULE
