##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261781_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23160903
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.688763775747432	32.0	32.0	32.0	32.0	32.0
2	31.436942074322406	32.0	32.0	32.0	32.0	32.0
3	35.2378260467651	37.0	32.0	37.0	32.0	37.0
4	36.03494932818466	37.0	37.0	37.0	32.0	37.0
5	36.2825972286141	37.0	37.0	37.0	37.0	37.0
6	39.63046898473691	41.0	41.0	41.0	37.0	41.0
7	39.75438971442521	41.0	41.0	41.0	37.0	41.0
8	39.93326702331079	41.0	41.0	41.0	37.0	41.0
9	39.98834428001361	41.0	41.0	41.0	37.0	41.0
10-14	40.03023383846475	41.0	41.0	41.0	37.0	41.0
15-19	39.98980539748385	41.0	41.0	41.0	37.0	41.0
20-24	39.93765716302166	41.0	41.0	41.0	37.0	41.0
25-29	39.850753893317545	41.0	41.0	41.0	37.0	41.0
30-34	39.80651621398354	41.0	41.0	41.0	37.0	41.0
35-39	39.71846908559653	41.0	41.0	41.0	37.0	41.0
40-44	39.59270804769572	41.0	41.0	41.0	37.0	41.0
45-49	39.56772070588094	41.0	41.0	41.0	37.0	41.0
50-54	39.6058175970082	41.0	41.0	41.0	37.0	41.0
55-59	39.55814927423167	41.0	41.0	41.0	37.0	41.0
60-64	39.501575573284	41.0	41.0	41.0	37.0	41.0
65-69	39.4806076257044	41.0	41.0	41.0	37.0	41.0
70-74	39.42963804994996	41.0	41.0	41.0	37.0	41.0
75-79	39.07153050984239	41.0	40.2	41.0	35.0	41.0
80-84	39.486522395089686	41.0	41.0	41.0	37.0	41.0
85-89	39.46798474135486	41.0	41.0	41.0	37.0	41.0
90-94	39.427911122463584	41.0	41.0	41.0	37.0	41.0
95-99	39.3904173511715	41.0	41.0	41.0	37.0	41.0
100-104	39.30300698552211	41.0	41.0	41.0	37.0	41.0
105-109	39.17980886151114	41.0	41.0	41.0	37.0	41.0
110-114	39.09321762627304	41.0	41.0	41.0	37.0	41.0
115-119	38.90146106997641	41.0	41.0	41.0	34.0	41.0
120-124	38.73479358727939	41.0	41.0	41.0	32.0	41.0
125-129	38.642148123499325	41.0	41.0	41.0	32.0	41.0
130-134	38.390637636192345	41.0	37.0	41.0	32.0	41.0
135-139	38.16849289511726	41.0	37.0	41.0	32.0	41.0
140-144	37.85769579882097	41.0	37.0	41.0	32.0	41.0
145-149	37.5508616309131	41.0	37.0	41.0	29.0	41.0
150	34.413414062482794	37.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	7.0
16	44.0
17	134.0
18	361.0
19	982.0
20	2488.0
21	5191.0
22	9447.0
23	16477.0
24	27434.0
25	43210.0
26	64534.0
27	92649.0
28	126037.0
29	166420.0
30	212158.0
31	264439.0
32	323932.0
33	393427.0
34	481355.0
35	593645.0
36	754409.0
37	1008296.0
38	1502570.0
39	3031259.0
40	1.4039998E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.95524403978008	11.240618713641535	4.579672216258482	33.224465030319905
2	25.82451192210137	12.47271630355323	40.107760649727524	21.595011124617873
3	23.45788935776813	16.24007924043376	26.01696056496588	34.285070836832226
4	26.0609614400613	25.690367081110782	24.23325204548372	24.015419433344203
5	27.743028844773455	28.933776891168705	24.825780756475687	18.497413507582152
6	22.831306029328637	29.91887833416834	26.099053209945623	21.150762426557392
7	18.186108719508905	22.196526620745313	42.99975696111676	16.617607698629023
8	19.43059387624049	20.95905328043557	34.30673665875636	25.30361618456759
9	19.778581171899905	19.210373619715952	36.43121772929147	24.579827479092675
10-14	24.62587969409814	25.42857848480102	26.935683651562297	23.009858169538546
15-19	24.842294716820877	24.54616190556186	27.24362280385076	23.367920573766508
20-24	24.544292453489643	24.700700911577	27.30139133910356	23.453615295829795
25-29	24.358794412843892	24.89489731121981	27.305979571255847	23.440328704680454
30-34	24.38506890200378	24.982338105866567	26.863701902441576	23.768891089688072
35-39	24.61892551269105	24.88259125876068	26.949147620271486	23.549335608276788
40-44	24.473895256266733	24.76781242853976	27.173908303420692	23.58438401177282
45-49	24.452659019372877	24.660902457488284	27.277947412903707	23.60849111023513
50-54	24.20090426760883	25.081583698327204	27.03603730628049	23.681474727783478
55-59	24.582557900116893	24.86954087042388	26.882654673905975	23.665246555553257
60-64	24.168629051184677	24.706473900926362	27.186751714161606	23.938145333727356
65-69	24.42115871836608	25.130881823966284	26.85358640139004	23.594373056277597
70-74	24.437547182857173	24.929032552891105	26.856977754729527	23.77644250952219
75-79	24.34985858351258	24.886876999692824	26.80712440799559	23.956140008799
80-84	24.245431512451802	24.85090223062787	26.994319740484595	23.909346516435736
85-89	24.636015987378656	24.760988516357614	26.76003431664837	23.842961179615358
90-94	24.431783557044938	24.944472383130524	26.797049203835815	23.826694855988723
95-99	24.6373186029563	25.01621035692439	26.647354504031988	23.699116536087324
100-104	24.478394771273706	25.004509776772917	26.658399706856308	23.858695745097073
105-109	24.679061639257903	25.109861052116443	26.29682145346915	23.914255855156505
110-114	24.873034898705246	25.20836706353752	26.213060477256917	23.705537560500318
115-119	24.70801834227537	25.63159062196871	25.920742305820017	23.7396487299359
120-124	24.968150966469825	25.61315126641795	25.633155004306136	23.78554276280609
125-129	24.799179334454024	25.84420324865632	25.4039076688223	23.952709748067353
130-134	24.863653261940666	25.84618585559654	25.192674170468383	24.09748671199441
135-139	24.813271279180608	26.17029993699222	24.757252080049113	24.259176703778063
140-144	24.80473117911873	26.28294400636111	24.553472346174807	24.35885246834535
145-149	24.615394406544464	26.397864760865364	24.404896492879878	24.581844339710297
150	24.451051692795076	26.127264601341693	24.412261382272682	25.009422323590545
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	121.0
1	93.5
2	66.0
3	67.0
4	79.5
5	98.5
6	112.5
7	112.5
8	115.5
9	146.0
10	165.0
11	185.5
12	244.5
13	290.0
14	362.0
15	472.5
16	646.0
17	929.0
18	1322.5
19	2013.0
20	3198.0
21	5423.0
22	8255.5
23	11501.0
24	16979.0
25	25237.0
26	35537.5
27	48268.0
28	66184.0
29	90662.0
30	116852.5
31	143376.5
32	180799.5
33	229315.5
34	284248.0
35	339866.5
36	383523.0
37	442052.5
38	492589.0
39	495949.0
40	507887.5
41	571175.0
42	639969.0
43	707111.5
44	783879.5
45	868019.0
46	943072.5
47	992609.0
48	1037088.0
49	1101968.5
50	1187205.5
51	1201715.0
52	1166254.5
53	1109428.5
54	1007163.0
55	932463.0
56	852848.5
57	753511.0
58	677806.5
59	599074.0
60	499913.5
61	397328.5
62	313354.5
63	241108.5
64	184947.0
65	137890.5
66	95915.5
67	70001.0
68	50727.5
69	34028.0
70	23083.0
71	15399.5
72	10873.0
73	7230.5
74	4492.0
75	2785.0
76	1800.0
77	1075.0
78	547.0
79	306.5
80	180.0
81	107.0
82	73.0
83	38.0
84	17.0
85	10.0
86	5.5
87	3.0
88	2.0
89	2.5
90	1.5
91	1.0
92	2.0
93	1.0
94	1.0
95	1.5
96	0.5
97	0.5
98	1.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.402143085699206
2	0.20810501214050245
3	0.0
4	0.0
5	0.0
6	0.0064418904565163105
7	0.0
8	0.0
9	0.0
10-14	4.749383044348487E-5
15-19	4.835735463336641E-5
20-24	3.108687083573555E-5
25-29	5.414296670557275E-4
30-34	1.0189585440602207E-4
35-39	2.746006923823307E-4
40-44	0.0012797428494044467
45-49	0.0026138877227714304
50-54	6.415984730819865E-4
55-59	3.7217892583894503E-4
60-64	5.811517797902785E-4
65-69	5.353849977265566E-4
70-74	9.222438347934879E-4
75-79	4.1362808695325913E-4
80-84	0.09162337064319125
85-89	5.742435862712261E-4
90-94	0.0011467601241626892
95-99	4.00675224105036E-4
100-104	4.809829737640195E-4
105-109	6.355538037528157E-4
110-114	0.0017477729603202431
115-119	0.0835312854598113
120-124	9.99961011882827E-4
125-129	8.160303594380582E-4
130-134	0.0018548499598655547
135-139	0.0011743928982388986
140-144	7.124074566522729E-4
145-149	0.0012667899865562236
150	0.0020724580557157034
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3160903E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.31830344510734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.08832493894086	27.27080570317275
2	11.963749207460689	8.690061481154396
3	4.216837428922569	4.594451439668884
4	2.253849308778274	3.2742393246301917
5	1.3164628053633238	2.3905847819691237
6	0.9041033644955723	1.9701300202495609
7	0.6445959953038901	1.6387443069863368
8	0.47007857470080194	1.3657965055221828
9	0.3726373377833581	1.2180200317733667
>10	2.3407157400573286	16.24363906765746
>50	0.23491447080617062	5.902834574408045
>100	0.16498113956874216	11.774711960144781
>500	0.016971530390436897	4.252732343317577
>1k	0.011150968920866101	7.591005921904298
>5k	5.41103810192806E-4	1.415888878679623
>10k+	8.608469707612642E-5	0.4063536587610212
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTGTGATGTGTTTAGGCTAAGGCGTCTTTGCATCTAGTGACAAGGTTTG	23531	0.10159793856051294	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.181145139289258E-5	0.0	0.0	0.0	4.3176209494077155E-6
2	6.476431424111573E-5	0.0	0.0	1.2952862848223146E-5	4.3176209494077155E-6
3	8.63524189881543E-5	0.0	0.0	1.2952862848223146E-5	1.7270483797630862E-5
4	1.2521100753282375E-4	0.0	0.0	1.2952862848223146E-5	3.4540967595261724E-5
5	1.5111673322927005E-4	0.0	0.0	1.2952862848223146E-5	3.4540967595261724E-5
6	1.8997532177393947E-4	0.0	0.0	1.2952862848223146E-5	3.4540967595261724E-5
7	2.0724580557157033E-4	0.0	0.0	1.7270483797630862E-5	3.4540967595261724E-5
8	2.547396360150552E-4	0.0	0.0	3.4540967595261724E-5	3.8858588544669434E-5
9	3.5404491785143264E-4	0.0	0.0	3.4540967595261724E-5	5.61290723423003E-5
10-11	4.5982663111192166E-4	0.0	0.0	3.4540967595261724E-5	5.61290723423003E-5
12-13	6.044669329170801E-4	0.0	0.0	3.4540967595261724E-5	5.828788281700416E-5
14-15	7.232015090257924E-4	4.3176209494077155E-6	0.0	3.8858588544669434E-5	6.0446693291708016E-5
16-17	8.419360851345044E-4	6.476431424111573E-6	0.0	4.749383044348487E-5	6.0446693291708016E-5
18-19	9.23970883173251E-4	8.635241898815431E-6	0.0	5.61290723423003E-5	6.0446693291708016E-5
20-21	9.908940078890707E-4	8.635241898815431E-6	0.0	5.61290723423003E-5	7.555836661463501E-5
22-23	0.0010707699954531132	1.2952862848223146E-5	0.0	6.0446693291708016E-5	7.987598756404273E-5
24-25	0.0011290578782701174	2.1588104747038575E-5	0.0	6.908193519052345E-5	8.419360851345044E-5
26-27	0.0011592812249159716	2.1588104747038575E-5	0.0	7.339955613993116E-5	8.851122946285816E-5
28-29	0.0012391572124800143	2.1588104747038575E-5	0.0	8.203479803874658E-5	9.498766088696973E-5
30-31	0.001366527030487542	2.1588104747038575E-5	0.0	8.63524189881543E-5	1.0362290278578517E-4
32-33	0.0015068497113432926	2.3746915221742433E-5	0.0	8.63524189881543E-5	1.0794052373519288E-4
34-35	0.0016320607188761165	2.590572569644629E-5	0.0	8.851122946285816E-5	1.1657576563400832E-4
36-37	0.0018155596092259442	2.590572569644629E-5	0.0	1.0146409231108131E-4	1.2521100753282375E-4
38-39	0.0020681404347662954	2.590572569644629E-5	0.0	1.1657576563400832E-4	1.619107856027893E-4
40-41	0.002409232489769505	2.806453617115015E-5	0.0	1.4248149133045461E-4	1.878165112992356E-4
42-43	0.002879853173254946	3.0223346645854008E-5	0.0	1.4248149133045461E-4	1.986105636727549E-4
44-45	0.003378538392911537	3.4540967595261724E-5	0.0	1.532755437039739E-4	2.0292818462216262E-4
46-47	0.004097422280987922	3.4540967595261724E-5	0.0	1.5543435417867774E-4	2.0292818462216262E-4
48-49	0.004909135019476572	3.4540967595261724E-5	0.0	1.5543435417867774E-4	2.0292818462216262E-4
50-51	0.0060511457605949125	3.8858588544669434E-5	0.0	1.5543435417867774E-4	2.0292818462216262E-4
52-53	0.007622759786179321	3.8858588544669434E-5	0.0	1.5975197512808545E-4	2.094046160462742E-4
54-55	0.009330378871670073	3.8858588544669434E-5	0.0	1.5975197512808545E-4	2.1588104747038577E-4
56-57	0.011420107411183407	4.101739901937329E-5	0.0	1.5975197512808545E-4	2.2235747889449734E-4
58-59	0.014228719838773125	4.317620949407715E-5	0.0	1.7054602750160474E-4	2.245162893692012E-4
60-61	0.0175727172640894	4.749383044348487E-5	0.0	1.727048379763086E-4	2.2667509984390506E-4
62-63	0.021698204081248472	4.749383044348487E-5	0.0	1.727048379763086E-4	2.353103417427205E-4
64-65	0.027118977183229857	5.397026186759644E-5	0.0	1.727048379763086E-4	2.4394558364153592E-4
66-67	0.033914912557597605	5.61290723423003E-5	0.0	1.7918126940042018E-4	2.5905725696446293E-4
68-69	0.04233643221941735	5.61290723423003E-5	0.0	1.8565770082453175E-4	2.784865512367976E-4
70-71	0.05261452888948242	5.828788281700416E-5	0.0	1.878165112992356E-4	2.806453617115015E-4
72-73	0.06541195738352688	6.0446693291708016E-5	0.0	1.9213413224864332E-4	2.806453617115015E-4
74-75	0.08227874362238813	6.0446693291708016E-5	0.0	1.9645175319805104E-4	2.806453617115015E-4
76-77	0.10294071867577875	6.0446693291708016E-5	0.0	2.0076937414745876E-4	2.828041721862054E-4
78-79	0.12898460824260607	6.0446693291708016E-5	2.1588104747038578E-6	2.0292818462216262E-4	2.8712179313561306E-4
80-81	0.16068242244268283	6.0446693291708016E-5	4.3176209494077155E-6	2.1156342652097805E-4	2.8928060361031694E-4
82-83	0.2012982827137612	6.0446693291708016E-5	4.3176209494077155E-6	2.1156342652097805E-4	2.9143941408502083E-4
84-85	0.25317881604184433	6.0446693291708016E-5	4.3176209494077155E-6	2.1803985794508962E-4	2.957570350344285E-4
86-87	0.3174358098214046	6.0446693291708016E-5	1.2952862848223146E-5	2.2019866841979348E-4	2.979158455091324E-4
88-89	0.3958934589035669	6.0446693291708016E-5	1.2952862848223146E-5	2.2235747889449734E-4	2.979158455091324E-4
90-91	0.49362928552483465	6.260550376641187E-5	1.2952862848223146E-5	2.3315153126801663E-4	3.0007465598383626E-4
92-93	0.6126509834266824	6.476431424111573E-5	1.2952862848223146E-5	2.396279626921282E-4	3.0655108740794775E-4
94-95	0.759499748347463	6.908193519052345E-5	1.5111673322927004E-5	2.461043941162398E-4	3.238215712055786E-4
96-97	0.9371957561412869	7.339955613993116E-5	1.7270483797630862E-5	2.504220150656475E-4	3.2813919215498634E-4
98-99	1.1470990574072175	7.771717708933887E-5	1.7270483797630862E-5	2.568984464897591E-4	3.302980026296902E-4
100-101	1.3974951667471687	7.771717708933887E-5	1.7270483797630862E-5	2.6337487791387065E-4	3.3245681310439405E-4
102-103	1.6927124991629212	7.771717708933887E-5	1.7270483797630862E-5	2.698513093379822E-4	3.3893324452850565E-4
104-105	2.0417317062292435	7.771717708933887E-5	1.7270483797630862E-5	2.720101198126861E-4	3.410920550032095E-4
106-107	2.446016461447984	7.771717708933887E-5	1.7270483797630862E-5	2.828041721862054E-4	3.497272969020249E-4
108-109	2.910624857761375	7.771717708933887E-5	1.7270483797630862E-5	2.9143941408502083E-4	3.497272969020249E-4
110-111	3.4314249319208323	8.203479803874658E-5	1.7270483797630862E-5	3.0007465598383626E-4	3.5404491785143264E-4
112-113	4.019750438918551	8.851122946285816E-5	1.7270483797630862E-5	3.043922769332439E-4	3.6268015975024807E-4
114-115	4.684413643112274	9.930528183637745E-5	1.7270483797630862E-5	3.0655108740794775E-4	3.669977806996558E-4
116-117	5.4223360807650725	9.930528183637745E-5	1.7270483797630862E-5	3.0655108740794775E-4	3.669977806996558E-4
118-119	6.233364476333241	9.930528183637745E-5	1.7270483797630862E-5	3.0655108740794775E-4	3.669977806996558E-4
120-121	7.10613916909889	9.930528183637745E-5	1.7270483797630862E-5	3.087098978826516E-4	3.669977806996558E-4
122-123	8.0513721766375	9.930528183637745E-5	1.7270483797630862E-5	3.1302751883205936E-4	3.669977806996558E-4
124-125	9.063383668590124	9.930528183637745E-5	1.7270483797630862E-5	3.238215712055786E-4	3.734742121237674E-4
126-127	10.135939000305816	1.0146409231108131E-4	1.7270483797630862E-5	3.2813919215498634E-4	3.7779183307317505E-4
128-129	11.271933136631159	1.0362290278578517E-4	1.7270483797630862E-5	3.2813919215498634E-4	3.821094540225828E-4
130-131	12.458385150181751	1.0362290278578517E-4	1.7270483797630862E-5	3.3245681310439405E-4	3.8858588544669437E-4
132-133	13.684416794975569	1.0794052373519288E-4	1.7270483797630862E-5	3.410920550032095E-4	4.0369755876962135E-4
134-135	14.969707787300003	1.1873457610871217E-4	1.7270483797630862E-5	3.454096759526172E-4	4.1449161114314066E-4
136-137	16.283767088010343	1.2521100753282375E-4	1.7270483797630862E-5	3.475684864273211E-4	4.447149577889947E-4
138-139	17.624254546552006	1.273698180075276E-4	1.9429294272334717E-5	3.562037283261365E-4	4.598266311119217E-4
>>END_MODULE
