##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261780_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23625496
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.62632932658853	32.0	32.0	32.0	32.0	32.0
2	31.31988306192598	32.0	32.0	32.0	32.0	32.0
3	34.84501222746815	37.0	32.0	37.0	32.0	37.0
4	35.57844719958472	37.0	37.0	37.0	32.0	37.0
5	35.90223684615976	37.0	37.0	37.0	37.0	37.0
6	39.117200290736754	41.0	41.0	41.0	37.0	41.0
7	39.318157045253145	41.0	41.0	41.0	37.0	41.0
8	39.42027794887354	41.0	41.0	41.0	37.0	41.0
9	39.393429539003115	41.0	41.0	41.0	37.0	41.0
10-14	39.41805151519359	41.0	41.0	41.0	37.0	41.0
15-19	39.3440876246577	41.0	41.0	41.0	37.0	41.0
20-24	39.30195679278014	41.0	41.0	41.0	37.0	41.0
25-29	39.22771871540814	41.0	41.0	41.0	37.0	41.0
30-34	39.21037382664897	41.0	41.0	41.0	37.0	41.0
35-39	39.10443100961775	41.0	41.0	41.0	37.0	41.0
40-44	38.97431910000959	41.0	41.0	41.0	37.0	41.0
45-49	38.90522058034253	41.0	41.0	41.0	35.0	41.0
50-54	38.847746383821956	41.0	41.0	41.0	32.0	41.0
55-59	38.733084782643296	41.0	41.0	41.0	32.0	41.0
60-64	38.68654845595622	41.0	41.0	41.0	32.0	41.0
65-69	38.57455717331818	41.0	41.0	41.0	32.0	41.0
70-74	38.480099101411454	41.0	41.0	41.0	32.0	41.0
75-79	37.91552731845291	41.0	38.6	41.0	31.0	41.0
80-84	38.59210114361197	41.0	41.0	41.0	32.0	41.0
85-89	38.53521162053063	41.0	41.0	41.0	32.0	41.0
90-94	38.449976008969294	41.0	41.0	41.0	32.0	41.0
95-99	38.29821176241124	41.0	40.2	41.0	32.0	41.0
100-104	38.0815303094589	41.0	37.0	41.0	32.0	41.0
105-109	37.91584223247631	41.0	37.0	41.0	31.0	41.0
110-114	37.686161382601234	41.0	37.0	41.0	27.0	41.0
115-119	37.32569849962092	41.0	37.0	41.0	27.0	41.0
120-124	36.98075237023595	41.0	37.0	41.0	27.0	41.0
125-129	36.633311376827805	41.0	37.0	41.0	26.0	41.0
130-134	36.219709893074835	41.0	36.0	41.0	22.0	41.0
135-139	35.71574332238358	41.0	32.0	41.0	22.0	41.0
140-144	35.20367085626477	41.0	32.0	41.0	22.0	41.0
145-149	34.50660295978548	41.0	32.0	41.0	18.0	41.0
150	29.553792352126703	32.0	22.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	20.0
13	180.0
14	1117.0
15	5497.0
16	16875.0
17	33885.0
18	47250.0
19	58354.0
20	71403.0
21	86469.0
22	102073.0
23	119279.0
24	137529.0
25	158690.0
26	182512.0
27	207735.0
28	238268.0
29	271283.0
30	313862.0
31	363471.0
32	425066.0
33	501595.0
34	600395.0
35	735701.0
36	935611.0
37	1272714.0
38	1973633.0
39	4127603.0
40	1.0637425E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.134525657620316	24.241004889418303	6.7192433227403106	28.905226130221077
2	27.953030626668294	25.366895305200792	29.092889793912008	17.5871842742189
3	21.45807647805574	26.081954004267256	28.513217246317286	23.94675227135972
4	23.579741987215847	34.86046599825883	21.14901630001757	20.41077571450775
5	26.257433304488707	36.08024794302139	18.676626726085132	18.98569202640477
6	22.58554910339237	36.40498806882192	19.004731159929932	22.00473166785578
7	21.1678562396232	20.46315780562678	34.43861009907758	23.930375855672438
8	20.311747105753884	23.66941629500604	26.559010655268363	29.45982594397172
9	21.393950840227863	22.0769121630293	29.02128277010565	27.50785422663719
10-14	24.682578824708287	26.933836079945127	23.634971170034216	24.748613925312366
15-19	24.543419652758992	26.407852471552467	24.286760082504895	24.761967793183643
20-24	24.361020734673037	26.674294943149402	24.437560004635717	24.527124317541844
25-29	24.17043528202629	26.4417181752129	24.697838120396376	24.690008422364436
30-34	24.18700147119268	26.610161232504993	24.57174023273465	24.631097063567672
35-39	24.04648132790888	26.710087464822475	24.598447054499577	24.64498415276907
40-44	24.079555853694135	26.50740172309941	24.775851242367317	24.637191180839142
45-49	24.00785507482772	26.453942185214878	24.804127247986248	24.734075491971154
50-54	24.068465850010842	26.571403228340344	24.69947923871985	24.66065168292896
55-59	24.154328423111	26.452417211125002	24.677422559475687	24.715831806288314
60-64	24.036886179878746	26.414294512568137	24.934773564630703	24.61404574292241
65-69	24.235784715122037	26.39853624400466	24.964720111928546	24.400958928944757
70-74	24.24787460130336	26.440705933755343	24.889476795854026	24.421942669087272
75-79	24.13963498771114	26.502444273353525	24.84817707989644	24.50974365903889
80-84	24.020418347194948	26.686843714833753	24.81692354761481	24.47581439035649
85-89	24.133621620241513	26.577086730632722	24.676095213104233	24.613196436021536
90-94	24.071686748798353	26.71630850932209	24.75963515446685	24.45236958741271
95-99	24.062539353800396	26.78998142102745	24.70758528450019	24.43989394067197
100-104	24.307506929965527	26.911469195696153	24.4833394060239	24.29768446831442
105-109	24.35031963159223	27.127984207225765	24.30278517175431	24.218910989427695
110-114	24.453176182922185	27.14676818212101	24.22108986442323	24.17896577053357
115-119	24.71611223492127	27.206017943084422	24.070521992758582	24.00734782923573
120-124	25.022882104220358	27.275687485752652	23.917661232571845	23.783769177455145
125-129	25.492913568787003	27.427770254161903	23.652950272241043	23.42636590481005
130-134	26.097827173204568	27.370485467779826	23.389167734267684	23.142519624747926
135-139	26.45773439759428	27.360945456602416	23.403201598814245	22.778118546989052
140-144	26.88059081037675	27.40104967948677	23.162239325494017	22.556120184642463
145-149	27.504582769464054	27.094163531770736	23.177859200323446	22.223394498441763
150	27.263240425323065	26.989014585491088	23.468057404992912	22.279687584192935
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	249.0
1	198.5
2	162.5
3	185.0
4	223.0
5	272.0
6	283.5
7	319.5
8	378.5
9	455.0
10	518.0
11	563.5
12	648.5
13	752.0
14	884.0
15	1062.5
16	1317.0
17	1669.5
18	2148.5
19	2912.0
20	4353.0
21	6599.0
22	9799.0
23	13663.0
24	19161.0
25	26762.0
26	37238.0
27	51324.0
28	68553.0
29	92854.5
30	118498.5
31	145655.5
32	180781.0
33	230779.0
34	286409.0
35	323492.5
36	365342.0
37	430161.0
38	470264.5
39	484279.0
40	506619.5
41	558778.5
42	626284.5
43	697306.0
44	760767.0
45	830609.5
46	907058.5
47	957212.5
48	1014570.0
49	1082949.0
50	1154404.0
51	1165034.0
52	1131913.0
53	1109832.5
54	1060033.0
55	1008009.0
56	944264.0
57	849425.5
58	751008.0
59	645415.5
60	542208.5
61	446715.0
62	356567.5
63	281278.0
64	214268.0
65	158314.5
66	117942.5
67	89760.0
68	71535.5
69	56239.5
70	41231.0
71	30612.5
72	24541.5
73	17893.0
74	11803.5
75	7752.5
76	4964.5
77	3122.5
78	1919.0
79	1340.5
80	911.5
81	552.5
82	359.0
83	259.5
84	194.0
85	140.5
86	103.0
87	81.0
88	57.5
89	48.5
90	43.0
91	34.0
92	28.0
93	22.5
94	21.0
95	14.5
96	11.5
97	12.5
98	13.0
99	13.0
100	16.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.5711670138057632
2	0.028363425682152874
3	0.0
4	0.0
5	2.878246450360238E-4
6	0.0
7	3.005227911405543E-4
8	0.0
9	0.0
10-14	7.695076539345458E-4
15-19	1.5915009784344845E-4
20-24	0.0010073862576260833
25-29	2.7004724048968116E-4
30-34	0.0024651334304261804
35-39	1.845463900525094E-4
40-44	3.8856327079863213E-4
45-49	4.5205400132128446E-4
50-54	2.8782464503602377E-5
55-59	0.0019292716648149947
60-64	4.4697474287947223E-4
65-69	5.824216346611306E-4
70-74	5.942732376920255E-4
75-79	4.6813831972035636E-4
80-84	1.6168972706435454E-4
85-89	9.963811976688235E-4
90-94	0.0011216695725668575
95-99	3.2845871257052126E-4
100-104	2.2772008680791296E-4
105-109	4.622125182049088E-4
110-114	2.624283528269629E-4
115-119	6.34907305226523E-5
120-124	1.9639799308340445E-4
125-129	1.506846671070948E-4
130-134	4.105733907131516E-4
135-139	7.932108599963362E-4
140-144	3.166071095396262E-4
145-149	1.8623947619978012E-4
150	0.0018750929081023315
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3625496E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.43336904211973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.85975058147575	24.359469295072756
2	12.45856424446429	8.330635522402641
3	4.747055413940434	4.7613016655298885
4	2.438579273915173	3.261196832130813
5	1.4860283105596193	2.4841466456988726
6	1.0123858820473146	2.030848248451185
7	0.7096590258596169	1.6608404473945029
8	0.5511483655084861	1.4741397360805046
9	0.4461609485842042	1.342499728157811
>10	2.7565965874522496	17.7598506472366
>50	0.29009929435810844	6.750828229027217
>100	0.21153802498428187	14.049284841038503
>500	0.021249896819850178	4.898828965263413
>1k	0.010920633203999167	6.292576089801114
>5k	2.5034098530693684E-4	0.49615393312575146
>10k+	1.3175841331940547E-5	0.04739917358837125
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5396292209060922E-5	0.0	0.0	0.0	0.0
2	2.5396292209060922E-5	0.0	0.0	0.0	0.0
3	2.9629007577237744E-5	0.0	0.0	0.0	0.0
4	8.465430736353642E-5	0.0	0.0	4.232715368176821E-6	0.0
5	1.1005059957259733E-4	0.0	0.0	1.2698146104530461E-5	0.0
6	1.439123225180119E-4	0.0	0.0	1.2698146104530461E-5	0.0
7	1.5237775325436555E-4	0.0	0.0	1.2698146104530461E-5	0.0
8	1.6930861472707283E-4	0.0	0.0	1.2698146104530461E-5	0.0
9	2.1163576840884103E-4	0.0	0.0	1.6930861472707283E-5	0.0
10-11	2.645447105110513E-4	0.0	0.0	2.3279934524972515E-5	0.0
12-13	3.618971639791182E-4	0.0	0.0	2.9629007577237744E-5	0.0
14-15	4.3808604060630095E-4	0.0	0.0	3.174536526132615E-5	0.0
16-17	5.142749172334837E-4	0.0	0.0	3.3861722945414565E-5	6.3490730522652306E-6
18-19	6.010455822811085E-4	0.0	0.0	3.809443831359139E-5	8.465430736353641E-6
20-21	7.005143934332638E-4	0.0	0.0	4.0210795997679794E-5	8.465430736353641E-6
22-23	7.809359854286234E-4	0.0	0.0	4.232715368176821E-5	8.465430736353641E-6
24-25	9.54477315523873E-4	0.0	0.0	4.232715368176821E-5	8.465430736353641E-6
26-27	0.0011428331494077416	0.0	0.0	4.232715368176821E-5	8.465430736353641E-6
28-29	0.0013608179908688478	4.232715368176821E-6	0.0	4.232715368176821E-5	8.465430736353641E-6
30-31	0.001625362701379899	4.232715368176821E-6	0.0	5.0792584418121845E-5	8.465430736353641E-6
32-33	0.0018814419811545966	4.232715368176821E-6	0.0	5.290894210221025E-5	8.465430736353641E-6
34-35	0.0022010119914519467	4.232715368176821E-6	0.0	5.925801515447549E-5	8.465430736353641E-6
36-37	0.002573490943851507	4.232715368176821E-6	0.0	6.13743728385639E-5	8.465430736353641E-6
38-39	0.003017926057510073	1.058178842044205E-5	0.0	6.34907305226523E-5	8.465430736353641E-6
40-41	0.003549131836216264	1.6930861472707283E-5	0.0	6.772344589082913E-5	8.465430736353641E-6
42-43	0.004207319075967759	1.9047219156795693E-5	0.0	6.983980357491754E-5	8.465430736353641E-6
44-45	0.004918415257821465	2.1163576840884104E-5	0.0	7.195616125900595E-5	8.465430736353641E-6
46-47	0.005712049389354619	2.1163576840884104E-5	0.0	8.888702273171323E-5	8.465430736353641E-6
48-49	0.006679224850983023	2.1163576840884104E-5	0.0	8.888702273171323E-5	8.465430736353641E-6
50-51	0.007989250257433748	2.1163576840884104E-5	0.0	8.888702273171323E-5	8.465430736353641E-6
52-53	0.009491864213136521	2.1163576840884104E-5	0.0	8.888702273171323E-5	8.465430736353641E-6
54-55	0.011286535529243493	2.1163576840884104E-5	0.0	9.311973809989006E-5	8.465430736353641E-6
56-57	0.013212421021763945	2.5396292209060922E-5	0.0	9.735245346806687E-5	8.465430736353641E-6
58-59	0.015809191900140424	2.5396292209060922E-5	0.0	9.735245346806687E-5	8.465430736353641E-6
60-61	0.018814419811545968	2.9629007577237744E-5	0.0	1.0158516883624369E-4	8.465430736353641E-6
62-63	0.02253920933554157	3.3861722945414565E-5	0.0	1.037015265203321E-4	1.058178842044205E-5
64-65	0.02728619962095187	3.3861722945414565E-5	0.0	1.1428331494077415E-4	1.2698146104530461E-5
66-67	0.03306597245619732	3.3861722945414565E-5	0.0	1.1428331494077415E-4	1.2698146104530461E-5
68-69	0.04006900003284587	3.597808062950298E-5	0.0	1.1851603030895098E-4	1.6930861472707283E-5
70-71	0.048970400452121726	3.809443831359139E-5	0.0	1.227487456771278E-4	1.9047219156795693E-5
72-73	0.060064347432113176	3.809443831359139E-5	0.0	1.248651033612162E-4	2.1163576840884104E-5
74-75	0.07425029298855779	3.809443831359139E-5	0.0	1.3121417641348145E-4	2.5396292209060922E-5
76-77	0.0915409352675601	3.809443831359139E-5	0.0	1.439123225180119E-4	3.174536526132615E-5
78-79	0.11338597928272066	4.0210795997679794E-5	0.0	1.460286802021003E-4	3.3861722945414565E-5
80-81	0.1406742952613566	4.232715368176821E-5	0.0	1.502613955702771E-4	3.3861722945414565E-5
82-83	0.1753190705498839	4.867622673403344E-5	0.0	1.5449411093845395E-4	3.3861722945414565E-5
84-85	0.21864514505854182	5.0792584418121845E-5	0.0	1.5872682630663077E-4	3.3861722945414565E-5
86-87	0.27317310078907975	5.5025299786298666E-5	0.0	1.65075899358896E-4	3.3861722945414565E-5
88-89	0.34006270175237807	5.5025299786298666E-5	0.0	1.8412311851569168E-4	3.597808062950298E-5
90-91	0.4217456429274543	5.925801515447549E-5	0.0	1.9470490693613375E-4	3.809443831359139E-5
92-93	0.523569960182	5.925801515447549E-5	0.0	1.9470490693613375E-4	3.809443831359139E-5
94-95	0.649004363760236	5.925801515447549E-5	0.0	1.9470490693613375E-4	3.809443831359139E-5
96-97	0.7987620662017001	5.925801515447549E-5	0.0	1.9470490693613375E-4	3.809443831359139E-5
98-99	0.9817296534218796	5.925801515447549E-5	0.0	1.9893762230431056E-4	3.809443831359139E-5
100-101	1.1987282722022006	5.925801515447549E-5	0.0	1.9893762230431056E-4	3.809443831359139E-5
102-103	1.4564688927589078	6.34907305226523E-5	0.0	2.074030530406642E-4	3.809443831359139E-5
104-105	1.7633280588056226	6.34907305226523E-5	0.0	2.074030530406642E-4	3.809443831359139E-5
106-107	2.1202919930231303	6.34907305226523E-5	0.0	2.2433391451337148E-4	3.809443831359139E-5
108-109	2.5315489672682427	6.34907305226523E-5	0.0	2.3279934524972514E-4	3.809443831359139E-5
110-111	2.993850372495883	6.34907305226523E-5	0.0	2.3703206061790195E-4	4.232715368176821E-5
112-113	3.513223595390336	6.34907305226523E-5	0.0	2.3703206061790195E-4	4.232715368176821E-5
114-115	4.105372009967537	6.34907305226523E-5	0.0	2.4338113367016717E-4	4.232715368176821E-5
116-117	4.767639587333955	6.34907305226523E-5	0.0	2.454974913542556E-4	4.232715368176821E-5
118-119	5.491427566219139	6.34907305226523E-5	0.0	2.454974913542556E-4	4.232715368176821E-5
120-121	6.2706154402007055	6.34907305226523E-5	0.0	2.454974913542556E-4	4.655986904994503E-5
122-123	7.115365958877646	6.772344589082913E-5	0.0	2.454974913542556E-4	5.0792584418121845E-5
124-125	8.033207006532265	7.195616125900595E-5	0.0	2.581956374587861E-4	5.0792584418121845E-5
126-127	9.006276524310856	7.195616125900595E-5	0.0	2.624283528269629E-4	5.0792584418121845E-5
128-129	10.04122834077219	7.195616125900595E-5	0.0	2.666610681951397E-4	5.0792584418121845E-5
130-131	11.119700936649119	7.195616125900595E-5	0.0	2.7512649893149334E-4	5.0792584418121845E-5
132-133	12.243017035494196	7.195616125900595E-5	0.0	2.7935921429967015E-4	5.0792584418121845E-5
134-135	13.419936241761866	7.195616125900595E-5	0.0	2.8359192966784697E-4	5.0792584418121845E-5
136-137	14.626981799662534	7.195616125900595E-5	0.0	2.878246450360238E-4	5.0792584418121845E-5
138-139	15.853125369304415	7.407251894309437E-5	0.0	2.8994100272011216E-4	5.290894210221025E-5
>>END_MODULE
