##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261780_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23625496
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.060270946269235	32.0	32.0	32.0	32.0	32.0
2	31.438832310652863	32.0	32.0	32.0	32.0	32.0
3	35.37778686212556	37.0	32.0	37.0	32.0	37.0
4	36.14223049539362	37.0	37.0	37.0	32.0	37.0
5	36.39731165855735	37.0	37.0	37.0	37.0	37.0
6	39.80193190441378	41.0	41.0	41.0	37.0	41.0
7	39.87586034172573	41.0	41.0	41.0	37.0	41.0
8	40.117900339531495	41.0	41.0	41.0	37.0	41.0
9	40.13776519231596	41.0	41.0	41.0	37.0	41.0
10-14	40.153747959407916	41.0	41.0	41.0	37.0	41.0
15-19	40.12940032243132	41.0	41.0	41.0	37.0	41.0
20-24	40.10808997195234	41.0	41.0	41.0	37.0	41.0
25-29	40.04846103548472	41.0	41.0	41.0	37.0	41.0
30-34	39.99913214097177	41.0	41.0	41.0	37.0	41.0
35-39	39.98634672474178	41.0	41.0	41.0	37.0	41.0
40-44	39.92045667104724	41.0	41.0	41.0	37.0	41.0
45-49	39.87690355368624	41.0	41.0	41.0	37.0	41.0
50-54	39.88314761307022	41.0	41.0	41.0	37.0	41.0
55-59	39.820394001463505	41.0	41.0	41.0	37.0	41.0
60-64	39.75880523312611	41.0	41.0	41.0	37.0	41.0
65-69	39.7359457172878	41.0	41.0	41.0	37.0	41.0
70-74	39.691846063253024	41.0	41.0	41.0	37.0	41.0
75-79	39.3775522427127	41.0	40.2	41.0	36.0	41.0
80-84	39.69915409183367	41.0	41.0	41.0	37.0	41.0
85-89	39.687321713795974	41.0	41.0	41.0	37.0	41.0
90-94	39.65350253810544	41.0	41.0	41.0	37.0	41.0
95-99	39.6291249504349	41.0	41.0	41.0	37.0	41.0
100-104	39.55764527441032	41.0	41.0	41.0	37.0	41.0
105-109	39.454670784477926	41.0	41.0	41.0	37.0	41.0
110-114	39.382803417121906	41.0	41.0	41.0	37.0	41.0
115-119	39.21396239046156	41.0	41.0	41.0	37.0	41.0
120-124	39.08325552191582	41.0	41.0	41.0	37.0	41.0
125-129	39.01129130156674	41.0	41.0	41.0	37.0	41.0
130-134	38.797472992736324	41.0	41.0	41.0	33.0	41.0
135-139	38.6160426261527	41.0	41.0	41.0	32.0	41.0
140-144	38.36022631651839	41.0	39.4	41.0	32.0	41.0
145-149	38.11360144142582	41.0	37.0	41.0	32.0	41.0
150	35.20866283611569	41.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	18.0
16	71.0
17	196.0
18	355.0
19	870.0
20	2052.0
21	4369.0
22	8020.0
23	13860.0
24	23447.0
25	36994.0
26	56512.0
27	80577.0
28	110239.0
29	145552.0
30	183538.0
31	228169.0
32	278041.0
33	337939.0
34	408128.0
35	501105.0
36	633367.0
37	842664.0
38	1245543.0
39	2485079.0
40	1.599879E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.87808255771297	11.90033347252199	4.716741773380954	30.50484219638409
2	26.899738795234324	12.254867880967591	38.235713430389005	22.60967989340908
3	23.42030406472736	16.66569032032174	26.23630843559856	33.677697179352336
4	25.71392363571965	25.80190909007794	24.439326056900562	24.04484121730185
5	27.561097553253482	29.19068873728619	24.474161304380658	18.774052405079665
6	23.1446468519807	30.057406958942362	25.733761359470204	21.06418482960673
7	18.484098704213448	22.25063126717001	42.43299696226484	16.832273066351707
8	19.646639376375422	21.103878623331337	33.6502099257514	25.59927207454184
9	19.86166554979417	19.53694009217838	35.72957367752195	24.8718206805055
10-14	24.859301064467648	25.3927869269014	26.5800355690539	23.16787643957705
15-19	24.918922298686542	24.601178131167917	26.985886020844745	23.494013549300796
20-24	24.705800188448993	24.707235079262478	27.07285533240346	23.514109399885065
25-29	24.478464111056283	24.864735571792913	27.146178635037593	23.510621682113207
30-34	24.575563396791086	24.90742555244971	26.7022104506713	23.81480060008791
35-39	24.686587521953143	24.802732702692573	26.74089667628249	23.76978309907179
40-44	24.61158604994663	24.752580446513324	26.951595597889305	23.68423790565074
45-49	24.54995647032596	24.72731836805619	26.940042794683805	23.782682366934047
50-54	24.343649679754037	24.97608754071391	26.822974761066725	23.857288018465326
55-59	24.642390054882274	24.825479398330412	26.7061412905436	23.82598925624371
60-64	24.335531802871834	24.7456100576464	26.90501984809161	24.013838291390158
65-69	24.537001415386257	24.983996641235372	26.69452092419529	23.78448101918308
70-74	24.561389711457707	24.842801410582695	26.683846538760374	23.911962339199224
75-79	24.475691174158644	24.827595501393994	26.595809477686444	24.100903846760918
80-84	24.408309659974933	24.799837186640183	26.75386440880144	24.03798874458344
85-89	24.711338757452804	24.70136218145432	26.63305332325376	23.954245737839116
90-94	24.585732153933247	24.853995013169463	26.586473210526485	23.973799622370805
95-99	24.752284506260878	24.917149513734575	26.390726918558215	23.939839061446328
100-104	24.610147494489272	24.92152715757587	26.483464124084776	23.984861223850082
105-109	24.78549007323236	24.97603669992063	26.183148894285065	24.055324332561945
110-114	24.888385001906535	25.094653341443816	26.146623142922508	23.870338513727134
115-119	24.82154583742767	25.459399525487992	25.820862205504575	23.89819243157977
120-124	24.974255709303282	25.479293580201873	25.568016495173367	23.978434215321478
125-129	24.82957011273556	25.711041732530067	25.406051575161815	24.053336579572555
130-134	24.84579764846017	25.857464257645347	25.158787325783464	24.137950768111015
135-139	24.70142188343685	26.159525184793054	24.81952987961892	24.31952305215118
140-144	24.639542036579186	26.375650875525647	24.589021604938402	24.39578548295676
145-149	24.35966239205478	26.66964829384736	24.456268839683734	24.514420474414127
150	24.20645418644406	26.463639973827767	24.42321150975503	24.906694329973142
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	176.0
1	133.0
2	85.0
3	77.5
4	72.5
5	77.0
6	98.0
7	119.5
8	127.0
9	126.0
10	134.0
11	164.5
12	227.0
13	270.0
14	317.0
15	451.5
16	636.5
17	953.5
18	1445.5
19	2110.5
20	3415.5
21	5746.5
22	8767.5
23	12599.0
24	18742.0
25	27349.5
26	37894.5
27	50913.0
28	69050.5
29	93940.5
30	120631.0
31	146593.5
32	179902.5
33	226349.0
34	280091.5
35	332456.5
36	377987.0
37	438435.0
38	488915.5
39	495717.0
40	513756.5
41	573250.5
42	635090.0
43	697106.0
44	776365.0
45	856097.5
46	919308.0
47	964121.0
48	1017546.5
49	1089423.0
50	1156024.5
51	1171911.0
52	1155758.0
53	1129458.5
54	1063447.0
55	1007867.5
56	935683.0
57	832775.5
58	750540.5
59	663273.0
60	551311.0
61	437027.0
62	343265.0
63	262496.5
64	200003.5
65	147771.0
66	102272.0
67	74986.5
68	54995.0
69	37377.5
70	25956.0
71	18206.5
72	13353.5
73	9182.5
74	5816.0
75	3703.0
76	2437.0
77	1450.0
78	786.0
79	433.0
80	261.5
81	168.0
82	92.0
83	49.5
84	33.5
85	18.5
86	11.5
87	7.5
88	4.0
89	2.0
90	0.5
91	0.5
92	1.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.5225542778022523
2	0.21377328966977033
3	0.0
4	0.0
5	0.0
6	0.007216779702741478
7	0.0
8	0.0
9	0.0
10-14	6.433727359628767E-5
15-19	4.7406412123580386E-5
20-24	2.1163576840884104E-5
25-29	6.010455822811086E-4
30-34	1.0243171190987905E-4
35-39	2.734334127842226E-4
40-44	0.0012774334981157645
45-49	0.0029307321209256307
50-54	6.569174251410425E-4
55-59	3.826374692831846E-4
60-64	6.41679649815606E-4
65-69	6.052782976492853E-4
70-74	0.0010674908158541942
75-79	4.122664768604223E-4
80-84	0.09115660471212964
85-89	6.60303597435584E-4
90-94	0.001311295221061179
95-99	4.5120745824764905E-4
100-104	5.299359640957379E-4
105-109	8.567015905189884E-4
110-114	0.0018844048819123204
115-119	0.08261498509914882
120-124	0.0010911940219159844
125-129	8.846375119489554E-4
130-134	0.0021146645979411394
135-139	0.0014331974236646715
140-144	8.592412197398945E-4
145-149	0.0015440945663109042
150	0.0019385836386249839
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3625496E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.4306961053632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41099784248753	27.85255447138988
2	12.422007026255235	9.299287400368923
3	4.395654260634179	4.935971964421287
4	2.267990023289689	3.395697813270078
5	1.342431747633067	2.5124077393922475
6	0.8736797788251772	1.9621465376763687
7	0.6431461728889419	1.6851386264113355
8	0.4691840750274339	1.4049509223862247
9	0.38905752797647464	1.3106424687472136
>10	2.35853981366612	16.94355529204614
>50	0.23718314846601685	6.185886316910951
>100	0.16515192048863236	12.098107666688367
>500	0.016308108068745098	4.193396460601734
>1k	0.00839759635569591	5.581773065390007
>5k	2.5917715715607697E-4	0.5768587042253405
>10k+	1.1780779870724444E-5	0.06162455007373101
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.925801515447549E-5	8.465430736353641E-6	0.0	8.465430736353641E-6	0.0
2	7.195616125900595E-5	8.465430736353641E-6	4.232715368176821E-6	8.465430736353641E-6	4.232715368176821E-6
3	1.0581788420442052E-4	8.465430736353641E-6	4.232715368176821E-6	8.465430736353641E-6	2.1163576840884104E-5
4	1.7777404546342646E-4	8.465430736353641E-6	4.232715368176821E-6	1.2698146104530461E-5	2.5396292209060922E-5
5	2.2433391451337148E-4	8.465430736353641E-6	4.232715368176821E-6	1.2698146104530461E-5	2.5396292209060922E-5
6	2.962900757723774E-4	8.465430736353641E-6	4.232715368176821E-6	1.2698146104530461E-5	2.9629007577237744E-5
7	3.2591908334961517E-4	8.465430736353641E-6	4.232715368176821E-6	1.2698146104530461E-5	2.9629007577237744E-5
8	3.470826601904993E-4	8.465430736353641E-6	4.232715368176821E-6	1.2698146104530461E-5	3.809443831359139E-5
9	3.978752446086211E-4	8.465430736353641E-6	4.232715368176821E-6	2.1163576840884104E-5	3.809443831359139E-5
10-11	4.5713325976309664E-4	8.465430736353641E-6	4.232715368176821E-6	2.1163576840884104E-5	3.809443831359139E-5
12-13	5.714165747038708E-4	8.465430736353641E-6	4.232715368176821E-6	2.1163576840884104E-5	5.5025299786298666E-5
14-15	6.454890936469651E-4	8.465430736353641E-6	4.232715368176821E-6	2.1163576840884104E-5	5.925801515447549E-5
16-17	7.153288972218826E-4	1.058178842044205E-5	4.232715368176821E-6	2.1163576840884104E-5	6.13743728385639E-5
18-19	7.724705546922697E-4	1.2698146104530461E-5	4.232715368176821E-6	3.3861722945414565E-5	7.618887662718277E-5
20-21	8.46543073635364E-4	1.2698146104530461E-5	4.232715368176821E-6	4.23271536817682E-5	8.042159199535959E-5
22-23	9.121501618421048E-4	1.2698146104530461E-5	4.232715368176821E-6	5.5025299786298666E-5	8.677066504762482E-5
24-25	0.0010031535422579064	1.2698146104530461E-5	4.232715368176821E-6	5.5025299786298666E-5	8.888702273171323E-5
26-27	0.0010793424188850892	1.4814503788618872E-5	4.232715368176821E-6	5.5025299786298666E-5	8.888702273171323E-5
28-29	0.0011936257338258634	1.6930861472707283E-5	4.232715368176821E-6	5.925801515447549E-5	9.100338041580164E-5
30-31	0.0013142581218189028	1.6930861472707283E-5	4.232715368176821E-6	5.925801515447549E-5	9.311973809989005E-5
32-33	0.0014370068674960305	1.6930861472707283E-5	4.232715368176821E-6	5.925801515447549E-5	1.0158516883624369E-4
34-35	0.0015999664091708382	1.6930861472707283E-5	4.232715368176821E-6	6.34907305226523E-5	1.037015265203321E-4
36-37	0.0018137185352637675	1.6930861472707283E-5	4.232715368176821E-6	6.560708820674071E-5	1.1428331494077415E-4
38-39	0.0020761468880907304	1.6930861472707283E-5	4.232715368176821E-6	7.195616125900596E-5	1.4391232251801192E-4
40-41	0.002402065971440346	1.6930861472707283E-5	8.465430736353641E-6	8.888702273171323E-5	1.5449411093845395E-4
42-43	0.0028443847274148234	1.6930861472707283E-5	8.465430736353641E-6	1.0158516883624369E-4	1.65075899358896E-4
44-45	0.0033417287831756	2.5396292209060922E-5	8.465430736353641E-6	1.1005059957259733E-4	1.7354133009524965E-4
46-47	0.003972403373033946	2.5396292209060922E-5	8.465430736353641E-6	1.1428331494077415E-4	1.7989040314751487E-4
48-49	0.004793550154460249	2.5396292209060922E-5	8.465430736353641E-6	1.1639967262486257E-4	1.9893762230431056E-4
50-51	0.005851728996504454	2.5396292209060922E-5	8.465430736353641E-6	1.1851603030895098E-4	2.0317033767248738E-4
52-53	0.007153288972218827	2.5396292209060922E-5	8.465430736353641E-6	1.3756324946574667E-4	2.074030530406642E-4
54-55	0.008698230081603366	2.5396292209060922E-5	8.465430736353641E-6	1.4179596483392348E-4	2.074030530406642E-4
56-57	0.010353221790560502	2.9629007577237744E-5	8.465430736353641E-6	1.439123225180119E-4	2.0951941072475263E-4
58-59	0.012696029746846374	3.809443831359139E-5	8.465430736353641E-6	1.5661046862254236E-4	2.1163576840884103E-4
60-61	0.015432480232372687	3.809443831359139E-5	1.2698146104530461E-5	1.5661046862254236E-4	2.1163576840884103E-4
62-63	0.01890119047659359	3.809443831359139E-5	1.2698146104530461E-5	1.5872682630663077E-4	2.1163576840884103E-4
64-65	0.02340479962833373	4.232715368176821E-5	1.2698146104530461E-5	1.6295954167480758E-4	2.1375212609292944E-4
66-67	0.02903854378337708	4.232715368176821E-5	1.2698146104530461E-5	1.65075899358896E-4	2.3279934524972514E-4
68-69	0.035783375722566846	4.232715368176821E-5	1.4814503788618872E-5	1.65075899358896E-4	2.497302067224324E-4
70-71	0.044443511365856614	4.232715368176821E-5	1.6930861472707283E-5	1.6719225704298443E-4	2.6031199514287446E-4
72-73	0.05533640436585967	4.4443511365856616E-5	1.6930861472707283E-5	1.8200676083160328E-4	2.624283528269629E-4
74-75	0.06948637184167478	4.655986904994502E-5	1.6930861472707283E-5	1.862394761997801E-4	2.6877742587922814E-4
76-77	0.08671140703247035	4.655986904994502E-5	1.6930861472707283E-5	1.9047219156795693E-4	2.7512649893149334E-4
78-79	0.10846756402489921	4.867622673403344E-5	1.6930861472707283E-5	1.9258854925204534E-4	2.7512649893149334E-4
80-81	0.13567545841153983	5.0792584418121845E-5	1.6930861472707283E-5	1.9893762230431056E-4	2.7512649893149334E-4
82-83	0.1706609672872053	5.0792584418121845E-5	1.6930861472707283E-5	1.9893762230431056E-4	2.7512649893149334E-4
84-85	0.21435105531752646	5.0792584418121845E-5	1.6930861472707283E-5	2.0317033767248738E-4	2.7512649893149334E-4
86-87	0.2692599554312003	5.0792584418121845E-5	1.6930861472707283E-5	2.074030530406642E-4	2.8147557198375853E-4
88-89	0.33685007078793183	6.34907305226523E-5	1.6930861472707283E-5	2.1163576840884103E-4	2.8359192966784697E-4
90-91	0.41957849265894775	6.560708820674071E-5	1.6930861472707283E-5	2.1586848377701785E-4	2.8359192966784697E-4
92-93	0.5228123041310964	6.772344589082913E-5	1.6930861472707283E-5	2.2221755682928307E-4	2.8359192966784697E-4
94-95	0.6501408478365914	6.772344589082913E-5	1.6930861472707283E-5	2.3279934524972514E-4	2.9840643345646585E-4
96-97	0.8020974459118234	6.772344589082913E-5	1.6930861472707283E-5	2.4126477598607876E-4	3.195700102973499E-4
98-99	0.9870522929973617	6.772344589082913E-5	1.6930861472707283E-5	2.454974913542556E-4	3.2168636798143835E-4
100-101	1.2058900266051558	6.772344589082913E-5	1.6930861472707283E-5	2.497302067224324E-4	3.238027256655268E-4
102-103	1.4655184382160695	6.772344589082913E-5	1.6930861472707283E-5	2.497302067224324E-4	3.3861722945414567E-4
104-105	1.774946862491268	7.195616125900595E-5	1.6930861472707283E-5	2.497302067224324E-4	3.3861722945414567E-4
106-107	2.1363022389032595	7.195616125900595E-5	1.6930861472707283E-5	2.539629220906092E-4	3.470826601904993E-4
108-109	2.5515083365868807	7.195616125900595E-5	1.6930861472707283E-5	2.6031199514287446E-4	3.470826601904993E-4
110-111	3.018069969832591	7.407251894309437E-5	1.6930861472707283E-5	2.645447105110513E-4	3.555480909268529E-4
112-113	3.543019795224617	8.042159199535959E-5	1.6930861472707283E-5	2.8359192966784697E-4	3.555480909268529E-4
114-115	4.142334196920141	8.2537949679448E-5	1.6930861472707283E-5	2.9417371808828903E-4	3.5978080629502974E-4
116-117	4.813903166308128	8.465430736353642E-5	2.5396292209060922E-5	2.962900757723774E-4	3.6401352166320655E-4
118-119	5.547352318021175	8.465430736353642E-5	2.5396292209060922E-5	2.962900757723774E-4	3.6824623703138337E-4
120-121	6.3401504882691135	8.677066504762482E-5	2.5396292209060922E-5	3.0263914882464266E-4	3.6824623703138337E-4
122-123	7.200642475400304	8.888702273171323E-5	2.5396292209060922E-5	3.047555065087311E-4	3.724789523995602E-4
124-125	8.134864131529767	8.888702273171323E-5	2.5396292209060922E-5	3.0687186419281953E-4	3.8729345618817906E-4
126-127	9.127016846545782	8.888702273171323E-5	2.9629007577237744E-5	3.132209372450847E-4	3.936425292404443E-4
128-129	10.18217352981711	8.888702273171323E-5	2.9629007577237744E-5	3.132209372450847E-4	3.936425292404443E-4
130-131	11.283413901659461	8.888702273171323E-5	2.9629007577237744E-5	3.132209372450847E-4	3.9999160229270956E-4
132-133	12.433019395656284	9.523609578397847E-5	3.809443831359139E-5	3.1745365261326154E-4	4.084570330290632E-4
134-135	13.64027024025231	1.2063238799303938E-4	3.809443831359139E-5	3.2168636798143835E-4	4.1057339071315157E-4
136-137	14.880862183803465	1.227487456771278E-4	4.655986904994502E-5	3.2168636798143835E-4	4.4020239829038933E-4
138-139	16.145559441376385	1.2909781872939304E-4	4.867622673403344E-5	3.238027256655268E-4	4.5290054439491977E-4
>>END_MODULE
