##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261779_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23114415
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.658971641722275	32.0	32.0	32.0	32.0	32.0
2	31.345350725943096	32.0	32.0	32.0	32.0	32.0
3	34.90747635187825	37.0	32.0	37.0	32.0	37.0
4	35.6249690939615	37.0	37.0	37.0	32.0	37.0
5	35.93541662205165	37.0	37.0	37.0	37.0	37.0
6	39.16970258602694	41.0	41.0	41.0	37.0	41.0
7	39.37167334756255	41.0	41.0	41.0	37.0	41.0
8	39.47093525836583	41.0	41.0	41.0	37.0	41.0
9	39.44462721639289	41.0	41.0	41.0	37.0	41.0
10-14	39.473772223956345	41.0	41.0	41.0	37.0	41.0
15-19	39.40165254452687	41.0	41.0	41.0	37.0	41.0
20-24	39.36147452574509	41.0	41.0	41.0	37.0	41.0
25-29	39.2853286401581	41.0	41.0	41.0	37.0	41.0
30-34	39.27107043807944	41.0	41.0	41.0	37.0	41.0
35-39	39.174035812716866	41.0	41.0	41.0	37.0	41.0
40-44	39.05302030789012	41.0	41.0	41.0	37.0	41.0
45-49	38.986716055760006	41.0	41.0	41.0	37.0	41.0
50-54	38.93143618819685	41.0	41.0	41.0	35.0	41.0
55-59	38.82203960602074	41.0	41.0	41.0	32.0	41.0
60-64	38.780405102184076	41.0	41.0	41.0	32.0	41.0
65-69	38.668130506439375	41.0	41.0	41.0	32.0	41.0
70-74	38.57674555034164	41.0	41.0	41.0	32.0	41.0
75-79	38.018332698448134	41.0	39.4	41.0	31.0	41.0
80-84	38.683454934939945	41.0	41.0	41.0	32.0	41.0
85-89	38.63005666377454	41.0	41.0	41.0	32.0	41.0
90-94	38.548476377187136	41.0	41.0	41.0	32.0	41.0
95-99	38.404937256685926	41.0	41.0	41.0	32.0	41.0
100-104	38.19531244896313	41.0	37.0	41.0	32.0	41.0
105-109	38.036323350601776	41.0	37.0	41.0	32.0	41.0
110-114	37.8141789095679	41.0	37.0	41.0	28.0	41.0
115-119	37.46261727151649	41.0	37.0	41.0	27.0	41.0
120-124	37.131013776468066	41.0	37.0	41.0	27.0	41.0
125-129	36.794237526668965	41.0	37.0	41.0	27.0	41.0
130-134	36.389279711383566	41.0	37.0	41.0	23.0	41.0
135-139	35.90184001628421	41.0	32.0	41.0	22.0	41.0
140-144	35.41078726846429	41.0	32.0	41.0	22.0	41.0
145-149	34.73287297126057	41.0	32.0	41.0	22.0	41.0
150	29.813465060655872	32.0	22.0	41.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	12.0
13	117.0
14	752.0
15	3895.0
16	14189.0
17	30506.0
18	43975.0
19	55000.0
20	67004.0
21	80075.0
22	95077.0
23	110898.0
24	128241.0
25	147219.0
26	169771.0
27	194397.0
28	221727.0
29	255689.0
30	294187.0
31	342414.0
32	399584.0
33	473180.0
34	568921.0
35	699154.0
36	891304.0
37	1210814.0
38	1882369.0
39	3943842.0
40	1.0790099E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.87592840975939	23.783333404374403	6.519518197404739	30.82121998846147
2	26.81535972467858	25.878999285498196	30.106244008404044	17.199396981419188
3	20.674540108412867	26.41683988108719	28.62538377025765	24.28323624024229
4	23.158626337720424	35.30826975287932	21.32540667804052	20.207697231359738
5	25.985874133951942	36.63382485371091	18.709303122932155	18.670997889404994
6	21.876984557039407	37.030320689491816	19.157798283019492	21.93489647044928
7	20.642478163557502	20.422644953509817	34.89207696243311	24.042799920499576
8	19.759721368678377	23.7831803227553	27.001366895939178	29.455731412627138
9	20.985411051934474	22.12086700009496	29.549854495560457	27.34386745241011
10-14	24.22835573079102	27.313791520098686	23.85049437953964	24.607358369570655
15-19	24.122130333208734	26.762598050316917	24.52070901553233	24.594562600942023
20-24	24.00133967976351	26.993055509828455	24.701583408065954	24.304021402342077
25-29	23.747636100722183	26.816338178115483	24.945227103922274	24.49079861724006
30-34	23.741939763390913	26.99250426506414	24.83743417839018	24.428121793154762
35-39	23.5182950756942	27.151002773477806	24.83796315684586	24.492738993982133
40-44	23.562870728523375	26.893901102707783	25.05221070883013	24.491017459938707
45-49	23.510942636360653	26.89153918539904	25.070785800191057	24.52673237804925
50-54	23.629432391106626	26.99483016844454	24.92163156688654	24.454105873562295
55-59	23.69470997411719	26.875469137738023	24.92502859983146	24.504792288313332
60-64	23.602190674297415	26.742208563996016	25.279355326400903	24.37624543530566
65-69	23.871734881338348	26.671294052452815	25.329627905955327	24.127343160253506
70-74	23.901408863716526	26.79265277852611	25.17203245236452	24.133905905392844
75-79	23.83342540188044	26.84743666775491	25.101388535247775	24.21774939511687
80-84	23.65950682738813	27.05304287372079	25.020599303629844	24.266850995261237
85-89	23.741064127708604	26.923905584670226	24.89500434962141	24.440025937999764
90-94	23.665187840759376	27.156393404326913	24.95641089491365	24.222007860000062
95-99	23.588858904034986	27.158133771904435	24.983773274148593	24.269234049911983
100-104	23.974177015049015	27.340061349066335	24.667146432052043	24.01861520383261
105-109	23.977742549383716	27.61144834376925	24.441313396973047	23.96949570987399
110-114	24.054074270809263	27.589720968660302	24.39717098771123	23.959033772819204
115-119	24.360371423547473	27.65245099085164	24.276308670420622	23.710868915180264
120-124	24.693439184255322	27.795061302472206	24.065344183126673	23.4461553301458
125-129	25.206659653082124	27.916471326517712	23.7867825767276	23.090086443672565
130-134	25.851717225034943	27.877438024286988	23.459406373380492	22.81143837729758
135-139	26.18990175588813	27.929779841952147	23.492836247327737	22.387482154831982
140-144	26.598790630935603	28.046448200436114	23.174659034946178	22.180102133682105
145-149	27.273978678304932	27.67848544310898	23.19255616042597	21.854979718160116
150	27.037894094441846	27.682634022513913	23.389589785329402	21.889882097714843
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	164.0
1	142.0
2	131.5
3	163.0
4	199.0
5	234.5
6	267.0
7	302.0
8	335.5
9	371.0
10	439.5
11	504.5
12	586.5
13	678.5
14	770.0
15	916.5
16	1163.0
17	1486.5
18	1899.0
19	2567.5
20	3874.5
21	5848.5
22	8805.5
23	12765.0
24	18171.5
25	25080.0
26	35075.0
27	49035.0
28	65811.0
29	90974.5
30	117651.5
31	146498.5
32	186035.5
33	247131.0
34	321601.0
35	365362.0
36	407813.5
37	497797.5
38	544119.0
39	530862.0
40	533262.5
41	580210.0
42	646518.5
43	718367.0
44	775448.5
45	839863.5
46	925725.0
47	980326.5
48	1035049.0
49	1108444.0
50	1186664.5
51	1169474.5
52	1092600.5
53	1040496.0
54	965280.5
55	907037.0
56	848264.5
57	749156.0
58	651580.0
59	550928.0
60	457648.5
61	377835.5
62	302725.0
63	240881.0
64	183897.5
65	137273.5
66	103340.5
67	78310.5
68	61407.5
69	47485.0
70	35203.0
71	26101.5
72	20347.0
73	14552.5
74	9630.5
75	6530.5
76	4286.5
77	2826.0
78	1826.0
79	1206.5
80	809.0
81	554.5
82	380.0
83	258.5
84	199.5
85	131.0
86	79.0
87	60.0
88	54.5
89	53.5
90	41.0
91	34.5
92	26.0
93	17.0
94	14.5
95	13.5
96	13.5
97	15.0
98	15.0
99	18.0
100	39.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.5471990097954025
2	0.025983785442980065
3	0.0
4	0.0
5	3.461043682048626E-4
6	0.0
7	3.1582023598693715E-4
8	0.0
9	0.0
10-14	7.337412605943088E-4
15-19	1.4190279096399367E-4
20-24	0.0010140857988402475
25-29	2.725571899613293E-4
30-34	0.002498008277518596
35-39	1.8257005422806503E-4
40-44	4.1359472000481086E-4
45-49	4.4993567866632143E-4
50-54	2.6823088535876852E-5
55-59	0.0020229800321574223
60-64	4.6377985339451596E-4
65-69	6.065479052790218E-4
70-74	6.160657754046555E-4
75-79	4.983902902150022E-4
80-84	1.7910901054601642E-4
85-89	9.491912298018358E-4
90-94	0.0011577191116452655
95-99	3.4004754176127755E-4
100-104	2.6650036351774425E-4
105-109	4.568577660304187E-4
110-114	2.509256669485254E-4
115-119	7.008613456148468E-5
120-124	1.9641422895625953E-4
125-129	1.4622909556655445E-4
130-134	4.0840315448173795E-4
135-139	7.562380445276249E-4
140-144	3.5216119464844773E-4
145-149	1.8430057606908936E-4
150	0.0019208792435369876
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3114415E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.856587048368922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.17563492607259	23.311259838420963
2	12.382957925452803	7.889575541369541
3	4.661305790771805	4.454798810483644
4	2.411878702559448	3.0733689535276865
5	1.5260506616915024	2.4307382872198167
6	0.9810768193318602	1.8752255457709386
7	0.710571085098495	1.5845458738545914
8	0.5263389165274833	1.3413889209041563
9	0.4161779490711653	1.1932208155977504
>10	2.6974082368532226	16.520060574043473
>50	0.26720840816084074	5.898724354505709
>100	0.20210733976527692	12.981704363980231
>500	0.023664841680871778	5.223275325031099
>1k	0.01667220699792907	9.960930199506569
>5k	7.767231053529284E-4	1.5924175936978395
>10k+	1.6946685934972761E-4	0.668765002086023
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4610436820486266E-5	0.0	0.0	4.326304602560783E-6	0.0
2	3.893674142304705E-5	0.0	0.0	1.2978913807682349E-5	0.0
3	5.1915655230729395E-5	0.0	0.0	1.2978913807682349E-5	0.0
4	8.652609205121565E-5	0.0	0.0	1.2978913807682349E-5	0.0
5	1.1681022426914114E-4	0.0	0.0	1.2978913807682349E-5	0.0
6	1.4276805188450585E-4	0.0	0.0	1.2978913807682349E-5	0.0
7	1.4709435648706663E-4	0.0	0.0	2.1631523012803913E-5	0.0
8	1.5142066108962741E-4	0.0	0.0	2.1631523012803913E-5	0.0
9	2.2496783933316072E-4	0.0	0.0	2.1631523012803913E-5	0.0
10-11	3.2014654058949796E-4	0.0	0.0	2.1631523012803913E-5	0.0
12-13	4.391199171599195E-4	0.0	0.0	2.1631523012803913E-5	0.0
14-15	5.234828569098547E-4	0.0	0.0	3.028413221792548E-5	0.0
16-17	6.208247104674724E-4	0.0	0.0	3.028413221792548E-5	0.0
18-19	7.203297163263704E-4	0.0	0.0	3.028413221792548E-5	0.0
20-21	8.306504836916703E-4	0.0	0.0	3.028413221792548E-5	2.1631523012803916E-6
22-23	9.323186418518487E-4	0.0	0.0	3.028413221792548E-5	4.326304602560783E-6
24-25	0.0010599446276273918	0.0	0.0	3.028413221792548E-5	4.326304602560783E-6
26-27	0.0012092021364157389	0.0	0.0	3.028413221792548E-5	4.326304602560783E-6
28-29	0.0013952332343258526	0.0	0.0	3.028413221792548E-5	4.326304602560783E-6
30-31	0.0016375062920692563	0.0	0.0	3.028413221792548E-5	4.326304602560783E-6
32-33	0.0019187160912357074	0.0	0.0	3.028413221792548E-5	4.326304602560783E-6
34-35	0.0022388626318252053	0.0	0.0	3.028413221792548E-5	4.326304602560783E-6
36-37	0.002606598523042872	4.326304602560783E-6	0.0	3.028413221792548E-5	4.326304602560783E-6
38-39	0.0030673499632155954	1.2978913807682349E-5	0.0	3.028413221792548E-5	4.326304602560783E-6
40-41	0.0036730326075741044	1.2978913807682349E-5	0.0	3.893674142304704E-5	4.326304602560783E-6
42-43	0.004432299065323522	1.7305218410243133E-5	0.0	4.758935062816861E-5	4.326304602560783E-6
44-45	0.005293233681233118	1.7305218410243133E-5	0.0	5.407880753200979E-5	4.326304602560783E-6
46-47	0.006199594495469602	1.7305218410243133E-5	0.0	6.489456903841175E-5	4.326304602560783E-6
48-49	0.007337412605943088	1.7305218410243133E-5	0.0	6.922087364097253E-5	4.326304602560783E-6
50-51	0.008797540409307351	1.7305218410243133E-5	0.0	6.922087364097253E-5	6.489456903841174E-6
52-53	0.010579977905562393	1.7305218410243133E-5	0.0	7.354717824353332E-5	8.652609205121566E-6
54-55	0.012624156830272364	1.7305218410243133E-5	0.0	8.003663514737449E-5	8.652609205121566E-6
56-57	0.014949545554148786	2.3794675314084305E-5	0.0	8.868924435249605E-5	8.652609205121566E-6
58-59	0.017975795623640052	2.5957827615364697E-5	0.0	9.085239665377644E-5	8.652609205121566E-6
60-61	0.02182836987222043	2.5957827615364697E-5	0.0	9.085239665377644E-5	8.652609205121566E-6
62-63	0.02633854242039005	2.5957827615364697E-5	0.0	9.085239665377644E-5	8.652609205121566E-6
64-65	0.03207089601878309	2.5957827615364697E-5	0.0	9.085239665377644E-5	1.2978913807682349E-5
66-67	0.03882209435107919	2.5957827615364697E-5	0.0	9.085239665377644E-5	1.2978913807682349E-5
68-69	0.047264877782976555	3.4610436820486266E-5	0.0	9.085239665377644E-5	1.2978913807682349E-5
70-71	0.05780808209941718	3.893674142304705E-5	0.0	1.0599446276273918E-4	1.514206610896274E-5
72-73	0.07079132221170209	3.893674142304705E-5	0.0	1.2113652887170193E-4	1.7305218410243133E-5
74-75	0.087084185344946	4.326304602560783E-5	0.0	1.276259857755431E-4	1.7305218410243133E-5
76-77	0.10761250068409692	4.758935062816861E-5	0.0	1.3844174728194506E-4	1.7305218410243133E-5
78-79	0.13338862350615405	4.758935062816861E-5	0.0	1.3844174728194506E-4	1.7305218410243133E-5
80-81	0.16475216872241846	4.758935062816861E-5	0.0	1.4060489958322547E-4	1.7305218410243133E-5
82-83	0.2042296982207856	4.9752502929449E-5	0.0	1.4276805188450585E-4	1.7305218410243133E-5
84-85	0.2546895519527533	5.1915655230729395E-5	0.0	1.4709435648706663E-4	1.7305218410243133E-5
86-87	0.3175896945693845	5.1915655230729395E-5	0.0	1.4709435648706663E-4	1.7305218410243133E-5
88-89	0.3939208498246657	5.1915655230729395E-5	0.0	1.6872587949987055E-4	1.7305218410243133E-5
90-91	0.4869515408458315	5.1915655230729395E-5	0.0	1.9035740251267444E-4	2.3794675314084305E-5
92-93	0.6009756249509235	5.1915655230729395E-5	0.0	1.9468370711523523E-4	2.5957827615364697E-5
94-95	0.7410267575450211	5.1915655230729395E-5	0.0	1.9468370711523523E-4	2.5957827615364697E-5
96-97	0.9089349654750076	5.1915655230729395E-5	0.0	2.011731640190764E-4	2.5957827615364697E-5
98-99	1.1093575156455397	5.1915655230729395E-5	0.0	2.0982577322419796E-4	2.5957827615364697E-5
100-101	1.3498676042634001	5.1915655230729395E-5	0.0	2.1631523012803915E-4	2.812097991664509E-5
102-103	1.6331086034407534	5.1915655230729395E-5	0.0	2.2064153473059993E-4	3.028413221792548E-5
104-105	1.9693987496547067	5.1915655230729395E-5	4.326304602560783E-6	2.2064153473059993E-4	3.028413221792548E-5
106-107	2.3564234699428908	6.922087364097253E-5	4.326304602560783E-6	2.3362044853828228E-4	3.028413221792548E-5
108-109	2.80230107489201	6.922087364097253E-5	4.326304602560783E-6	2.465993623459646E-4	3.028413221792548E-5
110-111	3.3000164615890126	7.138402594225292E-5	4.326304602560783E-6	2.509256669485254E-4	3.028413221792548E-5
112-113	3.8609218533110186	7.354717824353332E-5	4.326304602560783E-6	2.530888192498058E-4	3.028413221792548E-5
114-115	4.496386778553556	7.354717824353332E-5	4.326304602560783E-6	2.5957827615364693E-4	3.028413221792548E-5
116-117	5.202562989372649	7.354717824353332E-5	4.326304602560783E-6	2.6823088535876856E-4	3.028413221792548E-5
118-119	5.980763519215174	7.571033054481371E-5	4.326304602560783E-6	2.790466468651705E-4	3.028413221792548E-5
120-121	6.819032625311953	7.78734828460941E-5	4.326304602560783E-6	2.812097991664509E-4	3.028413221792548E-5
122-123	7.721305081699017	7.78734828460941E-5	4.326304602560783E-6	2.855361037690117E-4	3.028413221792548E-5
124-125	8.69171034611951	7.78734828460941E-5	4.326304602560783E-6	2.9635186527541364E-4	3.028413221792548E-5
126-127	9.719858798070382	7.78734828460941E-5	4.326304602560783E-6	2.98515017576694E-4	3.028413221792548E-5
128-129	10.811603927678897	7.78734828460941E-5	4.326304602560783E-6	3.09330779083096E-4	3.028413221792548E-5
130-131	11.949458811741504	8.219978744865487E-5	4.326304602560783E-6	3.2230969289077834E-4	3.028413221792548E-5
132-133	13.120974508764336	8.219978744865487E-5	4.326304602560783E-6	3.3528860669846067E-4	3.028413221792548E-5
134-135	14.344879591371878	8.219978744865487E-5	4.326304602560783E-6	3.461043682048626E-4	3.028413221792548E-5
136-137	15.598566954863447	8.219978744865487E-5	4.326304602560783E-6	3.461043682048626E-4	3.2447284519205874E-5
138-139	16.872962175335175	8.652609205121565E-5	6.489456903841174E-6	3.4826752050614305E-4	4.1099893724327435E-5
>>END_MODULE
