##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	SRR8261779_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23114415
Sequences flagged as poor quality	0
Sequence length	150
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.117133788590365	32.0	32.0	32.0	32.0	32.0
2	31.50923244217948	32.0	32.0	32.0	32.0	32.0
3	35.585654666146645	37.0	37.0	37.0	32.0	37.0
4	36.22619867299259	37.0	37.0	37.0	37.0	37.0
5	36.39419846879101	37.0	37.0	37.0	37.0	37.0
6	39.83073220758561	41.0	41.0	41.0	37.0	41.0
7	39.91938532729468	41.0	41.0	41.0	37.0	41.0
8	40.05540417094701	41.0	41.0	41.0	37.0	41.0
9	40.12181303312241	41.0	41.0	41.0	37.0	41.0
10-14	40.16503410534076	41.0	41.0	41.0	37.0	41.0
15-19	40.132503850951885	41.0	41.0	41.0	37.0	41.0
20-24	40.111548814884564	41.0	41.0	41.0	37.0	41.0
25-29	40.0529061713221	41.0	41.0	41.0	37.0	41.0
30-34	40.000638519296295	41.0	41.0	41.0	37.0	41.0
35-39	39.99005389493958	41.0	41.0	41.0	37.0	41.0
40-44	39.92541819466337	41.0	41.0	41.0	37.0	41.0
45-49	39.88366473475535	41.0	41.0	41.0	37.0	41.0
50-54	39.89033761832173	41.0	41.0	41.0	37.0	41.0
55-59	39.82839330348615	41.0	41.0	41.0	37.0	41.0
60-64	39.76731099619004	41.0	41.0	41.0	37.0	41.0
65-69	39.74487211551752	41.0	41.0	41.0	37.0	41.0
70-74	39.70204782599949	41.0	41.0	41.0	37.0	41.0
75-79	39.39084535775619	41.0	40.2	41.0	36.0	41.0
80-84	39.70832178101847	41.0	41.0	41.0	37.0	41.0
85-89	39.69700691105528	41.0	41.0	41.0	37.0	41.0
90-94	39.6647993384215	41.0	41.0	41.0	37.0	41.0
95-99	39.63770303509736	41.0	41.0	41.0	37.0	41.0
100-104	39.567616121801045	41.0	41.0	41.0	37.0	41.0
105-109	39.46626792847667	41.0	41.0	41.0	37.0	41.0
110-114	39.393671706595214	41.0	41.0	41.0	37.0	41.0
115-119	39.225635414091165	41.0	41.0	41.0	37.0	41.0
120-124	39.096697216866616	41.0	41.0	41.0	37.0	41.0
125-129	39.024971352292496	41.0	41.0	41.0	37.0	41.0
130-134	38.8116187842089	41.0	41.0	41.0	33.0	41.0
135-139	38.63132338845694	41.0	41.0	41.0	32.0	41.0
140-144	38.37527402705195	41.0	39.4	41.0	32.0	41.0
145-149	38.13205183864701	41.0	37.0	41.0	32.0	41.0
150	35.237809046865344	41.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	7.0
16	36.0
17	139.0
18	303.0
19	745.0
20	1946.0
21	4029.0
22	7768.0
23	13531.0
24	22922.0
25	36619.0
26	55276.0
27	79743.0
28	107359.0
29	141120.0
30	178305.0
31	221579.0
32	269803.0
33	325215.0
34	393896.0
35	484151.0
36	611533.0
37	816539.0
38	1208013.0
39	2417689.0
40	1.5716147E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.451334010323514	11.470108189590047	4.295524661534191	31.78303313855225
2	26.10114206641275	12.265125337950753	40.16599782849611	21.467734767140392
3	23.157886539633385	16.590556152946114	26.558980618804327	33.69257668861617
4	25.76755241263947	26.004980009228007	24.78253505442383	23.44493252370869
5	27.629580934667825	29.204628367189912	24.989860223587748	18.175930474554516
6	22.72650829109212	30.25410232306511	26.303109498644833	20.716279887197935
7	18.15407398370238	22.243370641221073	43.315961057201754	16.286594317874798
8	19.316188620823844	21.23356788393736	34.478445593366736	24.971797901872055
9	19.64580976849295	19.340411600293585	36.844384770282964	24.169393860930505
10-14	24.581079907248455	25.62929331461403	27.250013132508304	22.53961364562921
15-19	24.720087920254457	24.82984633544989	27.450930936526806	22.999134807768844
20-24	24.51830477336906	24.92357234500663	27.476277134158057	23.081845747466257
25-29	24.26701756078546	25.05263909309762	27.60759677402957	23.072746572087347
30-34	24.319008646665413	25.17112369963259	27.103041402294565	23.406826251407434
35-39	24.5459489333496	25.02509112369825	27.16521884885931	23.263741094092833
40-44	24.413758128533043	24.988308652946646	27.396409023529923	23.20152419499039
45-49	24.334264914422587	24.961501921518494	27.443497163225476	23.260736000833443
50-54	24.09853340555658	25.28095431181597	27.181570937319876	23.438941345307565
55-59	24.49946362264369	25.077482689954504	27.024765249461762	23.39828843794004
60-64	24.07675530978841	25.002525279488346	27.340697868016896	23.580021542706348
65-69	24.34637019821708	25.27923058071597	27.145343311128794	23.22905590993815
70-74	24.281694378473155	25.134240326957052	27.108068565047134	23.47599672952266
75-79	24.242170379051025	25.126770124675364	26.950024422524386	23.681035073749225
80-84	24.12024886599007	25.035254721958957	27.206572327169848	23.637924084881124
85-89	24.517472885436923	24.92384165591999	26.98100838649665	23.577677072146432
90-94	24.409056564473904	25.186383292239107	26.921452041873046	23.48310810141394
95-99	24.672321567407735	25.215948141175463	26.699156651902058	23.412573639514747
100-104	24.347735181411217	25.16288536415875	26.8522781713659	23.63710128306413
105-109	24.614480983378456	25.279822279617527	26.44907626304032	23.656620473963706
110-114	24.784701311959143	25.385734920101612	26.423147686106184	23.406416081833065
115-119	24.60884036687536	25.842116774283475	26.063302259274067	23.485740599567094
120-124	24.831154937408765	25.82161970797232	25.794110186652098	23.553115167966816
125-129	24.66072202538474	25.986287106804024	25.68490031274171	23.66809055506953
130-134	24.6402373688447	26.071141292654332	25.48596615406218	23.802655184438784
135-139	24.506599952998396	26.45238508920787	25.044867843460427	23.99614711433331
140-144	24.460974887278525	26.6732294927663	24.819053883916663	24.04674173603851
145-149	24.16326713061292	26.91615217446588	24.733226087327687	24.187354607593512
150	24.024271199692617	26.573447176706356	24.732050678076675	24.670230945524352
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	133.0
1	96.5
2	65.0
3	65.5
4	66.5
5	84.5
6	107.5
7	116.0
8	123.0
9	140.5
10	155.5
11	182.5
12	215.0
13	236.0
14	314.0
15	427.5
16	566.0
17	806.0
18	1254.0
19	1983.5
20	3177.5
21	5217.0
22	8111.5
23	11751.0
24	17353.5
25	25557.0
26	35981.5
27	49123.0
28	68193.5
29	95236.5
30	123628.0
31	152638.0
32	192004.0
33	245308.5
34	308528.5
35	373883.5
36	428788.0
37	512917.5
38	576810.5
39	554275.5
40	540939.5
41	597497.5
42	662976.5
43	730393.0
44	810000.5
45	881126.0
46	944469.5
47	988422.5
48	1029659.0
49	1091607.0
50	1152147.0
51	1146186.0
52	1107146.5
53	1053667.5
54	961102.0
55	898968.5
56	828430.5
57	728354.5
58	647260.0
59	564761.5
60	468624.0
61	371938.0
62	290844.5
63	222026.5
64	170378.5
65	126822.5
66	89100.5
67	65135.0
68	46791.0
69	31541.0
70	21846.5
71	15174.0
72	11382.5
73	8112.0
74	5020.5
75	3185.5
76	2318.0
77	1497.5
78	750.5
79	387.0
80	268.0
81	216.0
82	158.5
83	91.0
84	55.5
85	35.0
86	20.5
87	10.0
88	2.5
89	1.0
90	1.5
91	2.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.5011195394735277
2	0.21326085907863124
3	0.0
4	0.0
5	0.0
6	0.007432591307199425
7	0.0
8	0.0
9	0.0
10-14	8.133452652814272E-5
15-19	6.143352535636311E-5
20-24	3.0284132217925478E-5
25-29	5.624195983329018E-4
30-34	1.0642709322299527E-4
35-39	2.8380558192798734E-4
40-44	0.0013498070359989644
45-49	0.00287266625610036
50-54	6.861519099661402E-4
55-59	3.9369371883303127E-4
60-64	6.13469992643119E-4
65-69	5.658806420149504E-4
70-74	0.0010192773643633204
75-79	4.187862855278838E-4
80-84	0.09108861288507626
85-89	6.004910788354366E-4
90-94	0.0012944303370861863
95-99	3.997505452766163E-4
100-104	5.286744224329276E-4
105-109	7.467201744019911E-4
110-114	0.001873289892908819
115-119	0.08279768274472878
120-124	0.0010210078862043447
125-129	8.531472676249865E-4
130-134	0.002077491470149688
135-139	0.0013454807313964035
140-144	8.038273951557934E-4
145-149	0.0014259499970040342
150	0.0018862688067165013
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
150	2.3114415E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.831363846653154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.30606153873914	25.881814654676234
2	12.243853987846622	8.529402662719617
3	4.500174118926001	4.7024160632881
4	2.31379927397096	3.2237113751921767
5	1.3777031575134433	2.3993639976016823
6	0.9209366761452564	1.9246488267925683
7	0.6340236432830707	1.5458735744601284
8	0.4778207266194616	1.3314518065883725
9	0.3631411664782219	1.1383831887570894
>10	2.4198968109823937	16.05137428255659
>50	0.2410464868964271	5.847478108502855
>100	0.16879340683368463	11.68587176003093
>500	0.01866833583884196	4.476259250685006
>1k	0.01314939000510037	8.836922295929634
>5k	8.536732610147942E-4	2.0354012197460287
>10k+	7.760666009225031E-5	0.38962693247294505
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.354717824353332E-5	4.326304602560783E-6	0.0	4.326304602560783E-6	0.0
2	8.219978744865487E-5	4.326304602560783E-6	0.0	1.7305218410243133E-5	4.326304602560783E-6
3	9.517870125633722E-5	4.326304602560783E-6	0.0	1.7305218410243133E-5	4.326304602560783E-6
4	1.4276805188450585E-4	4.326304602560783E-6	0.0	2.5957827615364697E-5	4.326304602560783E-6
5	1.730521841024313E-4	4.326304602560783E-6	0.0	3.028413221792548E-5	4.326304602560783E-6
6	2.0766262092291758E-4	4.326304602560783E-6	0.0	3.028413221792548E-5	1.7305218410243133E-5
7	2.1198892552547836E-4	4.326304602560783E-6	0.0	3.4610436820486266E-5	1.7305218410243133E-5
8	2.292941439357215E-4	4.326304602560783E-6	0.0	4.326304602560783E-5	3.028413221792548E-5
9	3.1582023598693715E-4	4.326304602560783E-6	0.0	4.326304602560783E-5	3.4610436820486266E-5
10-11	4.261410033522371E-4	4.326304602560783E-6	0.0	4.326304602560783E-5	3.677358912176666E-5
12-13	5.407880753200979E-4	4.326304602560783E-6	0.0	4.326304602560783E-5	5.407880753200979E-5
14-15	6.208247104674724E-4	4.326304602560783E-6	0.0	4.9752502929449E-5	6.489456903841175E-5
16-17	7.116771071212488E-4	6.489456903841174E-6	0.0	5.624195983329018E-5	6.489456903841175E-5
18-19	8.025295037750252E-4	1.0815761506401957E-5	0.0	6.489456903841175E-5	6.489456903841175E-5
20-21	8.652609205121566E-4	1.514206610896274E-5	2.1631523012803916E-6	6.922087364097253E-5	6.922087364097253E-5
22-23	9.041976619352036E-4	1.946837071152352E-5	6.489456903841174E-6	8.436293974993526E-5	6.922087364097253E-5
24-25	9.66929078672335E-4	2.5957827615364697E-5	8.652609205121566E-6	9.734185355761761E-5	6.922087364097253E-5
26-27	0.0010361499523133075	2.5957827615364697E-5	8.652609205121566E-6	1.0383131046145879E-4	7.138402594225292E-5
28-29	0.001116186587460682	2.5957827615364697E-5	8.652609205121566E-6	1.0383131046145879E-4	7.78734828460941E-5
30-31	0.0012286705071272623	2.5957827615364697E-5	8.652609205121566E-6	1.0599446276273918E-4	7.78734828460941E-5
32-33	0.001354133340601525	2.5957827615364697E-5	8.652609205121566E-6	1.0815761506401957E-4	8.219978744865487E-5
34-35	0.0015293486770052368	2.812097991664509E-5	8.652609205121566E-6	1.1248391966658036E-4	8.652609205121565E-5
36-37	0.001756479668639678	3.028413221792548E-5	8.652609205121566E-6	1.2113652887170193E-4	9.517870125633722E-5
38-39	0.0020355263155048486	3.028413221792548E-5	8.652609205121566E-6	1.2113652887170193E-4	1.3411544267938428E-4
40-41	0.002444362100446842	3.4610436820486266E-5	1.0815761506401957E-5	1.2113652887170193E-4	1.4060489958322547E-4
42-43	0.0029938027849720616	3.4610436820486266E-5	1.2978913807682349E-5	1.2329968117298233E-4	1.4276805188450585E-4
44-45	0.003627606409247216	3.4610436820486266E-5	1.2978913807682349E-5	1.297891380768235E-4	1.4709435648706663E-4
46-47	0.004334957211765904	3.4610436820486266E-5	1.2978913807682349E-5	1.3627859498066466E-4	1.5142066108962741E-4
48-49	0.005293233681233118	3.4610436820486266E-5	1.2978913807682349E-5	1.4493120418578622E-4	1.6872587949987052E-4
50-51	0.006567330386687269	3.4610436820486266E-5	1.2978913807682349E-5	1.4709435648706663E-4	1.730521841024313E-4
52-53	0.00812047373900659	3.677358912176666E-5	1.2978913807682349E-5	1.6007327029474898E-4	1.795416410062725E-4
54-55	0.009969968956601325	3.893674142304705E-5	1.7305218410243133E-5	1.6007327029474898E-4	1.8170479330755288E-4
56-57	0.012053084622734342	3.893674142304705E-5	1.7305218410243133E-5	1.6007327029474898E-4	1.8170479330755288E-4
58-59	0.014867345766700131	3.893674142304705E-5	1.7305218410243133E-5	1.6656272719859014E-4	1.8170479330755288E-4
60-61	0.018453852282223022	3.893674142304705E-5	1.7305218410243133E-5	1.730521841024313E-4	1.8170479330755288E-4
62-63	0.02270228340193771	3.893674142304705E-5	1.7305218410243133E-5	1.730521841024313E-4	1.8386794560883325E-4
64-65	0.028261584816228313	3.893674142304705E-5	1.7305218410243133E-5	1.773784887049921E-4	1.9684685941651563E-4
66-67	0.034800794222998935	4.326304602560783E-5	1.7305218410243133E-5	1.8170479330755288E-4	2.0982577322419796E-4
68-69	0.04312027797372332	4.326304602560783E-5	1.7305218410243133E-5	1.8170479330755288E-4	2.1631523012803915E-4
70-71	0.053488266953760244	4.758935062816861E-5	1.946837071152352E-5	1.8603109791011366E-4	2.1631523012803915E-4
72-73	0.06623356031290431	4.758935062816861E-5	2.1631523012803913E-5	2.011731640190764E-4	2.1631523012803915E-4
74-75	0.0825156076846418	4.758935062816861E-5	2.1631523012803913E-5	2.0766262092291758E-4	2.1847838242931953E-4
76-77	0.10295739693174151	4.758935062816861E-5	2.1631523012803913E-5	2.0766262092291758E-4	2.271309916344411E-4
78-79	0.1285756096358052	5.1915655230729395E-5	2.1631523012803913E-5	2.0982577322419796E-4	2.292941439357215E-4
80-81	0.1598504656077171	5.1915655230729395E-5	2.1631523012803913E-5	2.1198892552547836E-4	2.3578360083956266E-4
82-83	0.1993842370659175	5.1915655230729395E-5	2.1631523012803913E-5	2.1847838242931953E-4	2.4227305774340385E-4
84-85	0.25009069016023117	6.922087364097253E-5	2.1631523012803913E-5	2.2064153473059993E-4	2.4227305774340385E-4
86-87	0.31289349092330476	6.922087364097253E-5	2.1631523012803913E-5	2.3794675314084307E-4	2.4227305774340385E-4
88-89	0.38975678164470096	7.138402594225292E-5	2.1631523012803913E-5	2.3794675314084307E-4	2.4227305774340385E-4
90-91	0.48370897554621217	7.787348284609409E-5	2.3794675314084305E-5	2.4227305774340385E-4	2.509256669485254E-4
92-93	0.5991845348454633	8.219978744865487E-5	3.028413221792548E-5	2.530888192498058E-4	2.552519715510862E-4
94-95	0.7404989483835087	8.219978744865487E-5	3.028413221792548E-5	2.59578276153647E-4	2.768834945638901E-4
96-97	0.90963150051602	8.219978744865487E-5	3.028413221792548E-5	2.6174142845492737E-4	2.8769925607029207E-4
98-99	1.1113562683719227	8.219978744865487E-5	3.028413221792548E-5	2.6390458075620775E-4	2.920255606728529E-4
100-101	1.3528938543328914	8.219978744865487E-5	3.028413221792548E-5	2.7039403766004893E-4	2.9418871297413326E-4
102-103	1.6374695184801347	8.436293974993526E-5	3.028413221792548E-5	2.725571899613293E-4	2.9635186527541364E-4
104-105	1.9757194806790483	8.652609205121565E-5	3.028413221792548E-5	2.7472034226260975E-4	2.98515017576694E-4
106-107	2.3661641447555564	8.652609205121565E-5	3.028413221792548E-5	2.855361037690117E-4	2.98515017576694E-4
108-109	2.814150823198424	8.652609205121565E-5	3.028413221792548E-5	2.920255606728529E-4	3.0284132217925483E-4
110-111	3.31513256987036	8.868924435249605E-5	3.028413221792548E-5	3.050044744805352E-4	3.071676267818156E-4
112-113	3.8806130287095737	9.085239665377644E-5	3.028413221792548E-5	3.071676267818156E-4	3.114939313843764E-4
114-115	4.522160738223312	9.085239665377644E-5	3.028413221792548E-5	3.136570836856568E-4	3.114939313843764E-4
116-117	5.235693830019059	9.085239665377644E-5	3.028413221792548E-5	3.1798338828821753E-4	3.114939313843764E-4
118-119	6.022058096646616	9.085239665377644E-5	3.028413221792548E-5	3.244728451920587E-4	3.1582023598693715E-4
120-121	6.8707903704246895	9.085239665377644E-5	3.028413221792548E-5	3.244728451920587E-4	3.1798338828821753E-4
122-123	7.784728707172558	9.085239665377644E-5	3.028413221792548E-5	3.244728451920587E-4	3.2230969289077834E-4
124-125	8.768227532472702	9.085239665377644E-5	3.2447284519205874E-5	3.244728451920587E-4	3.287991497946195E-4
126-127	9.811632697604503	9.085239665377644E-5	3.4610436820486266E-5	3.244728451920587E-4	3.309623020958999E-4
128-129	10.920326125493549	9.085239665377644E-5	3.4610436820486266E-5	3.266359974933391E-4	3.331254543971803E-4
130-131	12.077510938520401	9.085239665377644E-5	3.4610436820486266E-5	3.287991497946195E-4	3.374517589997411E-4
132-133	13.271203272935958	9.517870125633722E-5	3.893674142304705E-5	3.331254543971803E-4	3.4177806360230186E-4
134-135	14.523123340997383	1.0383131046145879E-4	3.893674142304705E-5	3.331254543971803E-4	3.525938251087038E-4
136-137	15.805485451394725	1.0815761506401957E-4	3.893674142304705E-5	3.331254543971803E-4	3.6773589121766656E-4
138-139	17.115494811354733	1.1032076736529997E-4	4.1099893724327435E-5	3.3528860669846067E-4	3.742253481215077E-4
>>END_MODULE
